scholarly journals Anti-Inflammatory Effects of the Nicotinergic Peptides SLURP-1 and SLURP-2 on Human Intestinal Epithelial Cells and Immunocytes

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Alex I. Chernyavsky ◽  
Valentin Galitovskiy ◽  
Igor B. Shchepotin ◽  
Sergei A. Grando
Keyword(s):  
T Cells ◽  
Epithelial Cells ◽  
Intestinal Epithelial Cells ◽  
Intestinal Epithelial ◽  
Therapeutic Modalities ◽  
Human Intestinal Epithelial Cells ◽  
Inflammatory Bowel ◽  
Anti Inflammatory ◽  
Contemporary Medicine ◽  
Inflammatory Action

A search for novel and more efficient therapeutic modalities of inflammatory bowel disease (IBD) is one of the most important tasks of contemporary medicine. The anti-inflammatory action of nicotine in IBD might be therapeutic, but its toxicity due to off-target and nonreceptor effects limited its use and prompted a search for nontoxic nicotinergic drugs. We tested the hypothesis that SLURP-1 and -2—the physiological nicotinergic substances produced by the human intestinal epithelial cells (IEC) and immunocytes—can mimic the anti-inflammatory effects of nicotine. We used human CCL-241 enterocytes, CCL-248 colonocytes, CCRF-CEM T-cells, and U937 macrophages. SLURP-1 diminished the TLR9-dependent secretion of IL-8 by CCL-241, and IFNγ-induced upregulation of ICAM-1 in both IEC types. rSLURP-2 inhibited IL-1β-induced secretion of IL-6 and TLR4- and TLR9-dependent induction of CXCL10 and IL-8, respectively, in CCL-241. rSLURP-1 decreased production of TNFαby T-cells, downregulated IL-1βand IL-6 secretion by macrophages, and moderately upregulated IL-10 production by both types of immunocytes. SLURP-2 downregulated TNFαand IFNγR in T-cells and reduced IL-6 production by macrophages. Combining both SLURPs amplified their anti-inflammatory effects. Learning the pharmacology of SLURP-1 and -2 actions on enterocytes, colonocytes, T cells, and macrophages may help develop novel effective treatments of IBD.

Liver X Receptor Exerts Anti-Inflammatory Effects in Colonic Epithelial Cells via ABCA1 and Its Expression Is Decreased in Human and Experimental Inflammatory Bowel Disease

2021 ◽  
Author(s):  
José Miranda-Bautista ◽  
Juan A Rodríguez-Feo ◽  
Marta Puerto ◽  
Beatriz López-Cauce ◽  
José M Lara ◽  
...  
Keyword(s):  
Inflammatory Bowel Disease ◽  
Epithelial Cells ◽  
Bowel Disease ◽  
Target Gene ◽  
Nuclear Factor Kappa B ◽  
Intestinal Epithelial Cells ◽  
Nuclear Factor ◽  
Intestinal Epithelial ◽  
Inflammatory Bowel ◽  
Anti Inflammatory

Abstract Background Liver X receptor (LXR) exerts anti-inflammatory effects in macrophages. The aim of this study was to explore the expression and function of LXR in the colonic epithelium under inflammatory conditions. Methods The expression of LXR was explored by Western blot and immunohistochemistry in colonic biopsies from patients diagnosed with inflammatory bowel disease (IBD) and control patients. In addition, LXR and its target gene expression were analyzed in the colon from interleukin (IL)-10-deficient (IL-10-/-) and wild-type mice. Caco-2 cells were pretreated with the synthetic LXR agonist GW3965 and further challenged with IL-1β, the expression of IL-8 and chemokine (C-C motif) ligand (CCL)-28 chemokines, the activation of mitogen-activated protein (MAP) kinases, and the nuclear translocation of the p65 subunit of nuclear factor kappa B was evaluated. Glibenclamide was used as an ABCA1 antagonist. Results We found that LXR expression was downregulated in colonic samples from patients with IBD and IL-10-/- mice. The nuclear positivity of LXR inversely correlated with ulcerative colitis histologic activity. Colonic IL-1β mRNA levels negatively correlated with both LXRα and LXRβ in the colon of IL-10-/- mice, where a decreased mRNA expression of the LXR target genes ABCA1 and FAS was shown. In addition, IL-1β decreased the expression of the LXR target gene ABCA1 in cultured intestinal epithelial cells. The synthetic LXR agonist GW3965 led to a decreased nuclear positivity of the p65 subunit of nuclear factor kappa B, a phosphorylation ratio of the p44-42 MAP kinase, and the expression of CCL-28 and IL-8 in IL-1β-stimulated Caco-2 cells. The pharmacological inhibition of ABCA1 increased the phosphorylation of p44-42 after GW3965 treatment and IL-1β stimulation. Conclusions The LXR-ABCA1 pathway exerts anti-inflammatory effects in intestinal epithelial cells and is impaired in the colonic mucosa of patients with IBD and IL-10-/- mice.

scholarly journals Anti-inflammatory effect of Lycium barbarum on polarized human intestinal epithelial cells

2019 ◽  
Vol 13 (2) ◽  
pp. 95 ◽  
Author(s):  
So-Rok Lee ◽  
Hye-Jeong Hwang ◽  
Ju-Gyeong Yoon ◽  
Eun Young Bae ◽  
Kyo-Suk Goo ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Intestinal Epithelial Cells ◽  
Intestinal Epithelial ◽  
Lycium Barbarum ◽  
Human Intestinal Epithelial Cells ◽  
Anti Inflammatory ◽  
Inflammatory Effect

scholarly journals Anti-inflammatory effect of Lactobacillus casei on Shigella-infected human intestinal epithelial cells.

2006 ◽  
Vol 176 (6) ◽  
pp. 3841.3-3841 ◽  
Author(s):  
M.-T. Tien ◽  
S. E. Girardin ◽  
B. Regnault ◽  
L. Le Bourhis ◽  
M.-A. Dillies ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Lactobacillus Casei ◽  
Intestinal Epithelial Cells ◽  
Intestinal Epithelial ◽  
Human Intestinal Epithelial Cells ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Carrageenan induces interleukin-8 production through distinct Bcl10 pathway in normal human colonic epithelial cells

2007 ◽  
Vol 292 (3) ◽  
pp. G829-G838 ◽  
Author(s):  
Alip Borthakur ◽  
Sumit Bhattacharyya ◽  
Pradeep K. Dudeja ◽  
Joanne K. Tobacman
Keyword(s):  
Molecular Weight ◽  
Epithelial Cells ◽  
High Molecular Weight ◽  
Intestinal Epithelial Cells ◽  
Epithelial Cell Line ◽  
Intestinal Epithelial ◽  
Human Intestinal Epithelial Cells ◽  
Normal Rat ◽  
Normal Human ◽  
Inflammatory Bowel

Carrageenan is a high molecular weight sulfated polygalactan used to improve the texture of commercial food products. Its use increased markedly during the last half century, although carrageenan is known to induce inflammation in rheumatological models and in intestinal models of colitis. We performed studies to determine its direct effects on human intestinal cells, including normal human intestinal epithelial cells from colonic surgeries, the normal intestinal epithelial cell line NCM460, and normal rat ileal epithelial cells. Cells were treated with high molecular weight λ-carrageenan at a concentration of 1 μg/ml for 1–96 h. IL-8, IL-8 promoter activity, total and nuclear NF-κB, IκBα, phospho-IκBα, and Bcl10 were assessed by immunohistochemistry, Western blot, ELISA, and cDNA microarray. Increased Bcl10, nuclear and cytoplasmic NF-κB, IL-8 promoter activation, and IL-8 secretion were detected following carrageenan exposure. Knockdown of Bcl10 by siRNA markedly reduced the increase in IL-8 that followed carrageenan exposure in the NCM460 cells. These results show, for the first time, that exposure of human intestinal epithelial cells to carrageenan triggers a distinct inflammatory pathway via activation of Bcl10 with NF-κB activation and upregulation of IL-8 secretion. Since Bcl10 contains a caspase-recruitment domain, similar to that found in NOD2/CARD15 and associated with genetic predisposition to Crohn's disease, the study findings may represent a link between genetic and environmental etiologies of inflammatory bowel disease. Because of the high use of carrageenan as a food additive in the diet, the findings may have clinical significance.

scholarly journals Anti-Inflammatory Effect ofLactobacillus caseionShigella-Infected Human Intestinal Epithelial Cells

2006 ◽  
Vol 176 (2) ◽  
pp. 1228-1237 ◽  
Author(s):  
Meng-Tsung Tien ◽  
Stephen E. Girardin ◽  
Béatrice Regnault ◽  
Lionel Le Bourhis ◽  
Marie-Agnès Dillies ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Intestinal Epithelial Cells ◽  
Intestinal Epithelial ◽  
Human Intestinal Epithelial Cells ◽  
Anti Inflammatory ◽  
Inflammatory Effect
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