scholarly journals Chlamydia trachomatisAntibody Testing in Vaginal Mucosal Material versus Blood Samples of Women Attending a Fertility Clinic and an STI Clinic

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Ingrid V. F. van den Broek ◽  
Jolande A. Land ◽  
Jan E. A. M. van Bergen ◽  
Servaas A. Morré ◽  
Marianne A. B. van der Sande
Keyword(s):  
Reproductive Tract ◽  
Epidemiological Studies ◽  
Vaginal Mucosa ◽  
Past Infection ◽  
Serum Igg ◽  
Serum Iga ◽  
Fertility Clinic ◽  
Iga Antibody ◽  
Tubal Pathology ◽  
Antibody Tests

Background.Chlamydia infections often follow an asymptomatic course but may damage the reproductive tract. Chlamydia antibodies in serum are used as markers for past infections and can relate to tubal pathology and infertility. This“proof of principle”study aimed to assess whether Chlamydia antibodies are detectable in easier to obtain, noninvasive, vaginal mucosa samples and relate to current or past infection.Methods.We compared outcomes of Chlamydia IgG and IgA antibody tests in serum and vaginal mucosal swabs in (a) 77 women attending a fertility clinic, of whom 25 tested positive for serum-IgG and (b) 107 women visiting an STI centre, including 30 Chlamydia PCR-positive subjects.Results.In the STI clinic, active Chlamydia infections were linked to serum-IgG and serum-IgA (P<0.001) and mucosa-IgA (P<0.001), but not mucosa-IgG. In the fertility clinic, mucosa-IgG had stronger correlations with serum-IgG (P=0.02) than mucosa-IgA (P=0.06). Women with tubal pathology or Chlamydia history more commonly had serum-IgG and mucosa-IgA (bothP<0.001), whereas this link was weaker for mucosa-IgG (P=0.03).Conclusion.Chlamydia IgG and IgA are detectable in vaginal mucosal material. Serum-IgG had stronger associations with current or past infections. Mucosa-IgA also showed associations with (past) infection and complications. IgA presence in vaginal mucosa warrants further epidemiological studies.

scholarly journals Role of serum IgA. Hepatobiliary transport of circulating antigen.

1981 ◽  
Vol 153 (4) ◽  
pp. 968-976 ◽  
Author(s):  
M W Russell ◽  
T A Brown ◽  
J Mestecky
Keyword(s):  
Molecular Weight ◽  
Human Serum Albumin ◽  
Gel Filtration ◽  
Low Molecular Weight ◽  
Serum Iga ◽  
Circulating Antigen ◽  
Circulating Antigens ◽  
Iga Antibody ◽  
Immune Spleen Cell

The IgA mediated hepatobiliary excretion of antigen from the circulation was studied using a radiolabeled haptenated protein (dinitrophenyl-human serum albumin) injected intravenously in mice together with monoclonal anti-dinitrophenyl antibodies of different immunoglobulin classes. Antibodies were obtained from ascitic fluids of mice bearing the MOPC315 myeloma (IgA), or immune spleen cell hybridomas (IgG and IgM). IgA antibody brought about the transport of large amounts of antigen from the circulation to the bile during 1-3h. Analysis of bile by gel filtration showed that a large part of the transported antigen remained intact and complexed with IgA. Neither IgA of different specificity nor anti-dinitrophenyl IgM medicated biliary transport of antigen. With anti-dinitrophenyl IgG, only small amounts of low molecular weight fragments of labeled antigen were found in he bile. Preformed immune complex of radiolabeled antigen and IgA antibody were rapidly transported from the circulation to the bile, resulting in threefold-higher levels of radioactivity in bile than in serum. It is proposed that an important function of serum IgA is to mediate the hepatobiliary excretion of corresponding circulating antigens.

A specific serum IgA antibody discriminates pneumonia from colonization state in patients with Pseudomonas aeruginosa in sputum culture

2010 ◽  
Vol 82 (3) ◽  
pp. 198-204 ◽  
Author(s):  
Masanori Yamashita ◽  
Yukio Hida ◽  
Hidehiro Sugimoto ◽  
Naoki Takahashi ◽  
Hideki Kimura ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Sputum Culture ◽  
Specific Serum ◽  
Serum Iga ◽  
Iga Antibody

scholarly journals P3.024 Comparison ofChlamydia TrachomatisAntibodies in Vaginal Mucosa and Serum in Women a Fertility Clinic and an STI-Clinic

2013 ◽  
Vol 89 (Suppl 1) ◽  
pp. A155.2-A155
Author(s):  
I V F van den Broek ◽  
J A Land ◽  
J E A M van Bergen ◽  
S A Morré ◽  
M A B van der Sande
Keyword(s):  
Vaginal Mucosa ◽  
Fertility Clinic

Serum IgA Antibody Response To Cryptosporidium parvum Is Mainly Represented By IgA1

1995 ◽  
Vol 171 (1) ◽  
pp. 256-256 ◽  
Author(s):  
L. Favennec ◽  
E. Comby ◽  
J. J. Ballet ◽  
P. Brasseur
Keyword(s):  
Antibody Response ◽  
Cryptosporidium Parvum ◽  
Serum Iga ◽  
Iga Antibody

scholarly journals REGULATION OF THE IMMUNE RESPONSE: SUPPRESSIVE AND ENHANCING EFFECTS OF PASSIVELY ADMINISTERED ANTIBODY

1971 ◽  
Vol 133 (5) ◽  
pp. 987-1003 ◽  
Author(s):  
Carolyn S. Pincus ◽  
Michael E. Lamm ◽  
Victor Nussenzweig
Keyword(s):  
Immune Response ◽  
Antibody Formation ◽  
Secretory Component ◽  
Secretory Iga ◽  
Antigenic Determinants ◽  
Antibody Synthesis ◽  
Serum Iga ◽  
Iga Antibody ◽  
Antigen Antibody

The ability of passively administered antibody to suppress the immune response against homologous antigenic determinants while concomitantly enhancing the response against other unrelated determinants of the same antigen molecule has been established in two distinct antigen-antibody systems: (a) guinea pig γ2-immunoglobulin + passive anti-F(ab')2 antibody, where suppression of anti-F(ab')2 antibody synthesis is accompanied by enhancement of the anti-Fc response; and (b) human secretory IgA + passive anti-serum IgA antibody, where suppression of antibody production against the α and L chains accompanies augmentation of the response to the secretory component. The mechanisms of the suppressive and enhancing effects are probably unrelated for the following reasons: (a) Enhancement of the response to certain determinants may be obtained without discernible suppression of the response to the homologous determinants; and (b) the F(ab')2 fragments of passive antibody can mediate immune suppression but were not observed to enhance the response against the unrelated determinants of the same antigen molecule. Also, the timing for achieving maximum suppression or enhancement of antibody formation is not the same; enhancement was obtained only at a later time. Both the enhancement and suppressive effects were obtained with the purified γG fraction of antisera. This finding rules out an exclusive role of γM antibody in the enhancement phenomenon.

Serum IgG, IgA, IgM, and IgE Levels after Electroacupuncture and Diet Therapy in Obese Women

2007 ◽  
Vol 35 (06) ◽  
pp. 955-965 ◽  
Author(s):  
Mehmet T. Cabioglu ◽  
Neyhan Ergene ◽  
H. Selcuk Surucu ◽  
H. Hamdi Çelik ◽  
Duygu Findik
Keyword(s):  
Immunoglobulin E ◽  
Immunoglobulin A ◽  
Diet Therapy ◽  
Immunoglobulin M ◽  
Blood Levels ◽  
Obese Women ◽  
Restricted Diet ◽  
Diet Restriction ◽  
Serum Igg ◽  
Serum Iga

We investigated the effect of acupuncture therapy on obese women's body weight and peripheral blood levels of serum immunoglobulin G ( IgG ), immunoglobulin A ( IgA ), immunoglobulin M ( IgM ), and immunoglobulin E ( IgE ). Sixty-three healthy women were assigned into 3 groups: placebo electroacupuncture ( n = 13; mean age, 40.5 ± 4.2 years; body mass index [BMI], 33.5 ± 4.2), diet restriction ( n = 23; mean age, 42.9 ± 4.0 years; BMI, 34.6 ± 2.9), and electroacupuncture ( n = 24; mean age, 40.1 ± 5.9 years; BMI, 33.9 ± 2.7). Electroacupuncture was applied to the Hunger and Shen Men ear points and to the LI 4, LI 11, St 36, St 44, and Ren 6 body points for 30 min once daily for 20 days. A restricted diet of 1400 kilocalories was given to participants in all 3 groups for 20 days. Weight was lost significantly in subjects in the electroacupuncture group compared to those in the diet restriction and placebo electroacupuncture groups ( p < 0.000, Tukey test). Modulations in serum IgG ( p < 0.001) were apparent in women treated with electroacupuncture compared with women treated with placebo electroacupuncture and restricted diet only. No significant changes were seen in serum IgA , IgM , and IgE levels among the 3 groups. Our results suggest that electroacupuncture can be effectively used to treat obesity. We also observed a modulating effect of serum IgG , which is associated with weight loss, in patients in the electroacupuncture group.

scholarly journals Diagnostic Properties of Three SARS-CoV-2 Antibody Tests

Diagnostics ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1441
Author(s):  
Suelen Basgalupp ◽  
Giovana dos Santos ◽  
Marina Bessel ◽  
Lara Garcia ◽  
Ana Carolina de Moura ◽  
...  
Keyword(s):  
Rapid Test ◽  
Epidemiological Studies ◽  
Antibody Detection ◽  
Sample Collection ◽  
Rt Pcr ◽  
Igg Antibody ◽  
Serological Tests ◽  
High Agreement ◽  
High Level ◽  
Antibody Tests

Serological assays emerged as complementary tools to RT-PCR in the diagnosis of SARS-CoV-2 as well as being needed for epidemiological studies. This study aimed to assess the performance of a rapid test (RT) compared to that of serological tests using finger prick blood samples. A total of 183 samples were evaluated, 88 of which were collected from individuals with negative RT-PCR and 95 from positive RT-PCR individuals. The diagnostic performance of RT (WONDFO®) and LUMIT (PROMEGA®) were compared to that of ELISA (EUROIMMUN®) for detecting antibodies against SARS-CoV-2 according to time from symptoms onset. The IgG antibody tests were detected in 77.4% (LUMIT), 77.9% (RT), and 80.0% (ELISA) of individuals. The detection of antibodies against SARS-CoV-2 increases in accordance with increasing time from symptoms onset. Considering only time from symptoms onset >21 days, the positivity rate ranged from 81.8 to 97.0% between the three tests. The RT and LUMIT showed high agreement with ELISA (agreement = 91.5%, k = 0.83, and agreement = 96.3%, k = 0.9, respectively) in individuals who had symptoms 15 to 21 days before sample collection. Compared to that of the ELISA assay, our results show sensitivity ranged from 95% to 100% for IgG antibody detection in individuals with symptoms onset between 15 and 21 days before sample collection. The specificity was 100% in individuals with symptoms onset >15 days before serological tests. This study shows good performance and high level of agreement of three immunoassays for the detection of SARS-CoV-2 antibodies.

scholarly journals Role ofToxoplasmaIgA as Part of a Reference Panel for the Diagnosis of Acute Toxoplasmosis during Pregnancy

2018 ◽  
Vol 57 (2) ◽  
Author(s):  
Tudor Rares Olariu ◽  
Brian G. Blackburn ◽  
Cindy Press ◽  
Jeanne Talucod ◽  
Jack S. Remington ◽  
...  
Keyword(s):  
Pregnant Women ◽  
The United States ◽  
Reference Laboratory ◽  
Content Type ◽  
Igm Antibody ◽  
Iga Antibodies ◽  
Iga Antibody ◽  
Acute Toxoplasmosis ◽  
Antibody Tests ◽  
Toxoplasmosis During Pregnancy

ABSTRACTThis study evaluated the usefulness of adding theToxoplasma gondiiIgA antibody enzyme-linked immunosorbent assay (ELISA) to the serologic panel of tests done for the diagnosis of acute toxoplasmosis in pregnant women in a reference laboratory in the United States. We conducted a retrospective study of 690 consecutive pregnant women with positiveT. gondiiIgG antibody test results who also hadT. gondiiIgA and IgM antibody tests performed. Patients were defined as acutely or chronically infected withT. gondiibased on a panel of serologic tests performed at the Palo Alto Medical Foundation Toxoplasma Serology Laboratory (PAMF-TSL). Among the 81 women who were positive byT. gondiiIgA antibody ELISA testing, 61 (75.3%) were acutely infected withT. gondii, while of the 547 who were negative by IgA testing, only 24 (4.4%) were acutely infected (P < 0.001). Among the 71 women who were positive by both IgA and IgM antibody tests, 61 (85.9%) were acutely infected, whereas 24 (19.2%) of the 125 women who were positive by only the IgM ELISA were acutely infected (P < 0.001). These results demonstrate that pregnant women withT. gondiiIgA antibodies are more likely than pregnant women withoutT. gondiiIgA antibodies to have had a recent infection withT. gondii.ToxoplasmaIgA antibody testing can therefore improve the accuracy of a serologic panel for the diagnosis of acute toxoplasmosis during pregnancy. Physicians who ordered testing only forT. gondiiIgG and IgM should also request additional testing for IgA and IgG avidity, if both IgG and IgM are positive. This further testing should, ideally, be performed in a reference laboratory.

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