scholarly journals Transforming Growth Factor-Beta (TGF-β) Signaling in Paravertebral Muscles in Juvenile and Adolescent Idiopathic Scoliosis

2014 ◽  
Vol 2014 ◽  
pp. 1-14 ◽  
Author(s):  
Roman Nowak ◽  
Magdalena Kwiecien ◽  
Magdalena Tkacz ◽  
Urszula Mazurek
Keyword(s):  
Adolescent Idiopathic Scoliosis ◽  
Idiopathic Scoliosis ◽  
Transforming Growth Factor Beta ◽  
Transforming Growth Factor ◽  
Pcr Analysis ◽  
Extracellular Region ◽  
Paravertebral Muscles ◽  
Growth Factor Beta ◽  
Genetic And Environmental Factors ◽  
Adolescent Period

Most researchers agree that idiopathic scoliosis (IS) is a multifactorial disease influenced by complex genetic and environmental factors. The onset of the spinal deformity that determines the natural course of the disease, usually occurs in the juvenile or adolescent period. Transforming growth factorsβ(TGF-βs) and their receptors, TGFBRs, may be considered as candidate genes related to IS susceptibility and natural history. This study explores the transcriptional profile of TGF-βs, TGFBRs, and TGF-βresponsive genes in the paravertebral muscles of patients with juvenile and adolescent idiopathic scoliosis (JIS and AIS, resp.). Muscle specimens were harvested intraoperatively and grouped according to the side of the curve and the age of scoliosis onset. The results of microarray and qRT-PCR analysis confirmed significantly higher transcript abundances of TGF-β2, TGF-β3, and TGFBR2 in samples from the curve concavity of AIS patients, suggesting a difference in TGF-βsignaling in the pathogenesis of juvenile and adolescent curves. Analysis of TGF-βresponsive genes in the transcriptomes of patients with AIS suggested overrepresentation of the genes localized in the extracellular region of curve concavity: LTBP3, LTBP4, ITGB4, and ITGB5. This finding suggests the extracellular region of paravertebral muscles as an interesting target for future molecular research into AIS pathogenesis.

Transforming Growth Factor Beta 1 Is a Novel Susceptibility Gene for Adolescent Idiopathic Scoliosis

Spine ◽  
2013 ◽  
Vol 38 (12) ◽  
pp. E699-E704 ◽  
Author(s):  
Igor I. Ryzhkov ◽  
Eugeny E. Borzilov ◽  
Mikhail I. Churnosov ◽  
Alexander V. Ataman ◽  
Andrey A. Dedkov ◽  
...  
Keyword(s):  
Growth Factor ◽  
Adolescent Idiopathic Scoliosis ◽  
Idiopathic Scoliosis ◽  
Transforming Growth Factor Beta ◽  
Transforming Growth Factor ◽  
Susceptibility Gene ◽  
Growth Factor Beta

Transforming growth factor-beta expression in macrophages during hypercholesterolemic states

1994 ◽  
Vol 267 (6) ◽  
pp. F937-F943 ◽  
Author(s):  
G. Ding ◽  
H. van Goor ◽  
J. Frye ◽  
J. R. Diamond
Keyword(s):  
Growth Factor ◽  
Transforming Growth Factor Beta ◽  
Transforming Growth Factor ◽  
Northern Analysis ◽  
Pcr Analysis ◽  
Tgf Beta ◽  
Glomerular Injury ◽  
Growth Factor Beta ◽  
Beta 2 ◽  
Mrna Isoform

Macrophage infiltration into the glomerular mesangium is a prominent feature of various glomerulopathies. Recent evidence suggests that infiltrating macrophages may play a role in propagating initial glomerular injury to the development of glomerulosclerosis via transforming growth factor-beta (TGF-beta)-stimulating matrix accumulation. Rats with the acute puromycin aminonucleoside (PA) nephrosis exhibit an elevated gene expression of glomerular TGF-beta 1; however, the cellular origin of this upregulation is unknown. Using polymerase chain reaction (PCR), we detected that the TGF-beta 1 isoform is expressed in glomerular macrophages isolated from experimental rats made hypercholesterolemic by either diet or by induction of PA nephrosis. Peritoneal macrophages from nephrotic or dietary-hypercholesterolemic animals also exhibited a significant increment in the expression of TGF-beta 1 mRNA on Northern analysis, in contrast to similar cells obtained from normal control rats. PCR analysis of glomerular RNA also detected the expression of the TGF-beta 2 mRNA isoform. TGF-beta 2 mRNA expression was not observed in isolated glomerular macrophages from either glomeruli of PA-nephrotic rats or from glomeruli of animals with dietary hypercholesterolemia. Expression of the TGF-beta 3 mRNA isoform was only observed by PCR in J774 A.1 cells. Thus the as a cellular source for the enhanced expression of TGF-beta 1 during the acute nephrotic phase of our toxic, progressive glomerulopathy model and within several days of inducing only hypercholesterolemia by dietary means.

Otosclerosis. 1. rész: patogenezis

2018 ◽  
Vol 159 (30) ◽  
pp. 1215-1220 ◽  
Author(s):  
Balázs Liktor ◽  
Andor Hirschberg ◽  
Tamás Karosi
Keyword(s):  
Transforming Growth Factor Beta ◽  
Measles Virus ◽  
Transforming Growth Factor ◽  
Clinical Symptoms ◽  
Stapes Fixation ◽  
The Past ◽  
Therapeutic Consequences ◽  
Growth Factor Beta ◽  
Genetic And Environmental Factors ◽  
Current Experience

Abstract: Otosclerosis can be found exclusively in the human otic capsule of the temporal bone. Its etiology is still unknown. In the past decades, several potential etiopathogenetic factors have been revealed, however, most studies were based on otosclerotic patients diagnosed by clinical symptoms only. The current experience indicates that one third of this group suffer from non-otosclerotic stapes fixation. In our experimental series, we have diagnosed and classified otosclerotic patients based on histologic examination, and analyzed also the pathogenetic factors. Recent data demonstrate that measles virus and rs1800472 SNP of transforming growth factor beta 1 (TGFβ1) gene are marked obvious etiologic factors, which have no therapeutic consequences so far. Furthermore, we summarize the genetic and environmental factors to be found in the literature, which may play a fundamental role in the pathogenesis of otosclerosis. Orv Hetil. 2018; 159(30): 1215–1220.

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