scholarly journals Antiosteoporotic Effect of Combined Extract ofMorus albaandPolygonum odoratum

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Sudarat Sungkamanee ◽  
Jintanaporn Wattanathorn ◽  
Supaporn Muchimapura ◽  
Wipawee Thukham-mee
Keyword(s):  
Oxidative Stress ◽  
Alkaline Phosphatase ◽  
Alkaline Phosphatase Level ◽  
Underlying Mechanism ◽  
Ovariectomized Rats ◽  
Bone Thickness ◽  
Osteoporosis Therapy ◽  
Tibia Bone ◽  
Stress Markers ◽  
Growth Formation

Due to the limitation of osteoporosis therapy, the alternative therapies from natural sources have been considered. In this study, we aimed to determine the antiosteoporotic effect of the combined extract ofMorus albaandPolygonum odoratumleaves. Ovariectomized rats, weighing 200–220 g, were orally given the combined extract at doses of 5, 150, and 300 mg·kg−1BW for 3 months. At the end of study, blood was collected to determine serum osteocalcin, calcium, and alkaline phosphatase level. In addition, tibia bone was isolated to determine bone oxidative stress markers, cortical bone thickness, and density of osteoblast. The combined extract decreased oxidative stress and osteoclast density but increased osteoblast density and cortical thickness. The elevation of serum calcium, alkaline phosphatase, and osteocalcin was also observed. These results suggested the antiosteoporotic effect of the combined extract via the increased growth formation together with the suppression of bone resorption. However, further studies concerning chronic toxicity and the underlying mechanism are required.

scholarly journals Rosmarinic and Sinapic Acids May Increase the Content of Reduced Glutathione in the Lenses of Estrogen-Deficient Rats

Nutrients ◽  
2019 ◽  
Vol 11 (4) ◽  
pp. 803 ◽  
Author(s):  
Maria Zych ◽  
Weronika Wojnar ◽  
Sławomir Dudek ◽  
Ilona Kaczmarczyk-Sedlak
Keyword(s):  
Oxidative Stress ◽  
Phenolic Acids ◽  
Rosmarinic Acid ◽  
Reduced Glutathione ◽  
Reductase Activity ◽  
Sinapic Acid ◽  
Ovariectomized Rats ◽  
Stress Markers ◽  
Female Wistar Rats ◽  
Cataract Development

Oxidative stress is believed to be associated with both postmenopausal disorders and cataract development. Previously, we have demonstrated that rosmarinic and sinapic acids, which are diet-derived antioxidative phenolic acids, counteracted some disorders induced by estrogen deficiency. Other studies have shown that some phenolic acids may reduce cataract development in various animal models. However, there is no data on the effect of phenolic acids on oxidative stress markers in the lenses of estrogen-deficient rats. The study aimed to investigate whether administration of rosmarinic acid and sinapic acid affects the antioxidative abilities and oxidative damage parameters in the lenses of estrogen-deficient rats. The study was conducted on three-month-old female Wistar rats. The ovariectomized rats were orally treated with rosmarinic acid at doses of 10 and 50 mg/kg or sinapic acid at doses of 5 and 25 mg/kg, for 4 weeks. The content of reduced glutathione (GSH), oxidized glutathione and amyloid β1-42, as well as products of protein and lipid oxidation, were assessed. Moreover, the activities of superoxide dismutase, catalase, and some glutathione-related enzymes in the lenses were determined. Rosmarinic and sinapic acids in both doses resulted in an increase in the GSH content and glutathione reductase activity. They also improved parameters connected with protein oxidation. Since GSH plays an important role in maintaining the lens transparency, the increase in GSH content in lenses after the use of rosmarinic and sinapic acids seems to be beneficial. Therefore, both the investigated dietary compounds may be helpful in preventing cataract.

scholarly journals Zingiberensis Newsaponin Inhibits the Malignant Progression of Hepatocellular Carcinoma via Suppressing Autophagy Moderated by the AKR1C1-Mediated JAK2/STAT3 Pathway

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Keqing He ◽  
Xing Liu ◽  
Shiping Cheng ◽  
Pingsheng Zhou
Keyword(s):  
Oxidative Stress ◽  
Hepatocellular Carcinoma ◽  
Protein Expression ◽  
Cell Viability ◽  
Underlying Mechanism ◽  
Anticancer Effect ◽  
Stat3 Pathway ◽  
Stress Markers ◽  
And Migration ◽  
Hcc Cells

Objective. Saponins are a group of compounds from various plants, which exhibit an anticancer activity. This study aimed to explore the anticancer effect of zingiberensis newsaponin (ZnS) against hepatocellular carcinoma (HCC) and the underlying mechanism involving autophagy. Methods. HCC cells (Huh7 and SMMC7721) were treated with ZnS and/or 3-MA. The cell viability, migration, and apoptosis were determined using CCK-8 assay, transwell assay, and flow cytometry, respectively. The levels of oxidative stress markers (ROS, SOD, and MDA) were measured by ELISA assay. Autophagy was monitored using MDC assay, immunofluorescence staining, and transmission electron microscopy. The relative protein expression of LC3II/LC3I, P62, AKR1C1, p-JAK2, p-STAT3, JAK2, and STAT3 was determined using Western blot. Results. ZnS or 3-MA inhibited the cell viability and migration, and it promoted cell apoptosis and oxidative stress in HCC. MDC-positive cells and autophagosomes were reduced by ZnS or 3-MA treatment. The expression of autophagy-related proteins LC3 (LC3II/LC3I) and P62 was, respectively, downregulated and upregulated after ZnS or 3-MA treatment. In addition, ZnS or 3-MA suppressed the protein expression of AKR1C1, p-JAK2, and p-STAT3 in HCC cells. Furthermore, the above phenomena were evidently enhanced by ZnS combined 3-MA treatment. AKR1C1 overexpression weakened the effect of ZnS on inhibiting the expression of AKR1C1, p-JAK2, and p-STAT3. Conclusion. ZnS exerts an anticancer effect on HCC via inhibiting autophagy moderated by the AKR1C1-mediated JAK2/STAT3 pathway. ZnS and 3-MA exert a synergistic effect on inhibiting HCC.

scholarly journals Gastroprotective and Antioxidative Effects of the Traditional Thai Polyherbal Formula Phy-Blica-D against Ethanol-Induced Gastric Ulcers in Rats

Nutrients ◽  
2021 ◽  
Vol 14 (1) ◽  
pp. 172
Author(s):  
Sineenart Sanpinit ◽  
Piriya Chonsut ◽  
Chuchard Punsawad ◽  
Palika Wetchakul
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Enzymes ◽  
Underlying Mechanism ◽  
Gastric Ulcers ◽  
Vehicle Group ◽  
Control Group ◽  
Antioxidant Enzyme Activities ◽  
Ulcer Area ◽  
Stress Markers ◽  
Antioxidative Effects

Phy-Blica-D is a traditional Thai polyherbal formula that has reduced oxidative stress in non-communicable diseases. However, evidence supporting the gastroprotective effects of Phy-Blica-D has not been previously reported. Therefore, this study aimed to evaluate the gastroprotective effects of Phy-Blica-D against gastric ulcers in rats and investigate the potential underlying mechanism. To estimate the possible mechanisms of action, we examined the levels of oxidative stress markers, such as reactive oxygen species (ROS) and malondialdehyde (MDA), as well as antioxidant enzymes, including catalase (CAT), superoxide dismutase (SOD), and glutathione (GSH). According to our results, rats treated with only 80% ethanol (vehicle group) exhibited significant increases in their ulcer area and ulcer index (UI). Moreover, the levels of ROS and MDA markedly increased in the vehicle group compared with the normal control group. Daily oral administration of Phy-Blica-D (500 and 1000 mg/kg) for 7 days not only significantly decreased the ulcer area and UI, but also remarkably decreased the ROS and MDA levels in gastric tissue. Gastric ulcers induced by ethanol had significantly decreased antioxidant enzyme activities (CAT and SOD) and non-enzymatic antioxidant (GSH), whereas pretreatment with Phy-Blica-D significantly improved the activities of CAT, SOD, and GSH. Moreover, after exposure to ethanol, the rats exhibited a significantly increased level of inducible nitric oxide synthase (iNOS), which was reduced after treatment with Phy-Blica-D. These findings suggest that Phy-Blica-D potentially exerts its gastroprotective effects by suppressing oxidative stress and stimulating antioxidant enzymes, which is one of the causes of destruction of cell membranes, and it is involved in the pathogenesis of acute gastric ulcers induced by ethanol.

EVALUATION OF HEPATO - PROTECTIVE ACTIVITY OF K-LIV DS ON CARBON TETRACHLORIDE INDUCED HEPATOTOXICITY IN RATS

INDIAN DRUGS ◽  
2018 ◽  
Vol 55 (08) ◽  
pp. 52-58
Author(s):  
D. Jain ◽  
◽  
K. Chainani
Keyword(s):  
Oxidative Stress ◽  
Alkaline Phosphatase ◽  
Carbon Tetrachloride ◽  
Histopathological Examination ◽  
Hepatoprotective Activity ◽  
Histopathological Study ◽  
Metabolic Function ◽  
Sleeping Time ◽  
Stress Markers ◽  
Ccl4 Administration

Present study investigates K-Liv DS, a polyherbal formulation on carbon tetrachloride induced hepatotoxicity in Wistar rats. Rats were divided into normal control, CCl4 control, CCl4+K-Liv DS (0.5 ml) and CCl4+K-LivDS (1 mL). CCl4 was injected intraperitoneally from day 1 to day 10, while K Liv DS was administered orally for 14 days. Hepatoprotective activity was assessed by estimating transaminases, alkaline phosphatase, total protein and bilirubin in serum. Estimation of oxidative stress markers and histopathological study of liver were also carried out. Metabolic function of liver was evaluated by thiopentone induced sleeping time. CCl4 administration produced a significant hepatotoxicity (P<0.001), which was reversed with K-Liv DS treatment. It significantly decreased transaminases, alkaline phosphatase and bilirubin and increased total proteins (P<0.001). Moreover oxidative stress was decreased and metabolic function was improved by decrease in sleeping time. Histopathological examination revealed no abnormalities in the K-Liv DS treated rats. K-Liv DS is an effective hepatoprotective agent and has potential clinical applications for liver diseases.

scholarly journals Curcumin Supplementation Decreases Intestinal Adiposity Accumulation, Serum Cholesterol Alterations, and Oxidative Stress in Ovariectomized Rats

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
Maurilio da Silva Morrone ◽  
Carlos Eduardo Schnorr ◽  
Guilherme Antônio Behr ◽  
Juciano Gasparotto ◽  
Rafael Calixto Bortolin ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Wistar Rats ◽  
Serum Levels ◽  
Antioxidant Defenses ◽  
Ovariectomized Rats ◽  
Oral Supplementation ◽  
Stress Markers ◽  
Female Wistar Rats ◽  
And Oxidative Stress ◽  
Hormone Deprivation

The aim of this study was to investigate the potential of curcumin oral supplementation (50 and 100 mg/Kg/day, for 30 days) in circumventing menopause-associated oxidative stress and lipid profile dysfunctions in a rat ovariectomy (OVX) model. Female Wistar rats were operated and randomly divided into either sham-operated or OVX groups. Sham-operated group (n=8) and one OVX group (n=11) were treated with vehicle (refined olive oil), and the other two OVX groups received curcumin at 50 or 100 mg/Kg/day doses (n=8/group). OVX vehicle-treated animals presented a higher deposition of intestinal adipose tissue as well as increased serum levels of IL-6, LDL, and total cholesterol when compared to sham-operated rats. In addition, several oxidative stress markers in serum, blood, and liver (such as TBARS, carbonyl, reduced-sulphydryl, and nonenzymatic antioxidant defenses) were altered toward a prooxidant status by OVX. Interestingly, curcumin supplementation attenuated most of these parameters to sham comparable values. Thus, the herein presented results show that curcumin may be useful to ameliorate lipid metabolism alterations and oxidative damage associated with hormone deprivation in menopause.

scholarly journals Genistein Enhances the Promoting Effects of Exercise on Anti-Oxidant and Anti- Inflammation Balance and Cardiomyopathy in the Ovariectomized Diabetic Rat

2021 ◽  
Author(s):  
Farzaneh Nazari-Serenjeh ◽  
Seyyed Alireza Vafaie ◽  
Maryam Khajvand-Abedini ◽  
Amir Mohammad Salehi ◽  
Mohammad Mohammadi ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cardiac Tissue ◽  
Ovariectomized Rats ◽  
Diabetic Rat ◽  
Cardiac Complications ◽  
Expression Levels ◽  
Stress Markers ◽  
Tnf Α ◽  
Anti Oxidant ◽  
Combined Intervention

Abstract Purpose: These experiments aimed to analyze the effects of genistein and/or exercise on the expression of miRNA-133, IGF-1, and Bcl-2, Bax plus oxidative stress and inflammation markers in the heart of diabetic ovariectomized rats. Methods: Animals were divided into seven groups (n=8): control, sham, ovariectomy (OVX), diabetic ovariectomized (OD), and diabetic ovariectomized with eight weeks of genistein administration (ODG) and with eight weeks of swimming training (ODS) and with eight weeks of both of them (ODGS). High-fat nutrition and low dose streptozotocin injection were used for induction of diabetes. The effect of those treatments was evaluated by measuring lipid profiles, miRNA-133 gene expression, and Bcl-2, Bax, and IGF-1 protein expression levels. Inflammation and oxidative stress markers levels were also measured.Results: ovariectomy down-regulated cardiac miRNA-133, IGF-1, and Bcl-2 expression levels and increased MDA, OSI, TOS, TNF- α, and NF-κB levels plus a reduction in TAC. Diabetes induced an additive effect on the above-measured factors. Genistein improved oxidative and inflammation conditions and up-regulated miRNA-133, Bcl-2, and IGF-1 expression in OD rats. Genistein also enhanced the positive effect of exercise on miRNA-133, Bcl-2, and IGF-1expression in the heart, along with a reduction in Bax. The combined intervention was associated with improvement in oxidative and inflammation conditions. Histological observation showed some abnormality in cardiac tissue, which was improved by genistein or/and exercise treatment.Conclusion: genistein or/and exercise as a natural replacement therapy could improve diabetic-induced cardiac complications within the heart of ovariectomized rats.

Acute Administration of Tibolone Prevents Oxidative Stress in Ovariectomized Rats Fed High-Fat-and-Fructose Diet

2018 ◽  
Vol 127 (06) ◽  
pp. 396-404 ◽  
Author(s):  
Norma Angélica Estrada Cruz ◽  
Julio César Almanza Pérez ◽  
Ángeles Fortis Barrera ◽  
Juan Manuel Gallardo ◽  
Leticia Manuel Apolinar ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Enzymatic Activity ◽  
Metabolic Parameters ◽  
Ovariectomized Rats ◽  
Acute Administration ◽  
Oxidative Stress Markers ◽  
High Fat ◽  
Glucose Levels ◽  
Stress Markers ◽  
And Oxidative Stress

AbstractIn addition to oxidative stress due to the increase of free radicals, estrogen deficiency is associated with changes in enzymatic activity, glutathione redox ratio (GSH/GSSG), and the content of oxidative markers such as malondialdehyde. Tibolone, a synthetic steroid, has been used as an elective treatment for the relief of menopausal symptoms. However, the acute effects of hormonal therapy with tibolone on metabolic parameters and oxidative stress markers associated with the first stages of estrogen deficiency are still unknown. The study aimed to evaluate if the acute administration of tibolone reduces oxidative stress in ovariectomized rats fed high-fat-and-fructose diet. Rats were fed a standard diet or a diet consisting of 10% lard-supplemented chow and 20% high-fructose syrup in the drinking water plus tibolone or vehicle for seven days. Weight, cholesterol, triglycerides, and glucose levels, as well as antioxidant enzymes and oxidative stress markers were quantified in the serum of each experimental group. It was observed that seven days of diet and tibolone treatment in the ovariectomized group reduced weight, triglycerides, cholesterol, glucose levels and advanced glycation end products but did not change GSH/GSSG ratio nor the enzymatic activity of superoxide dismutase. Also, both glutathione peroxidase and glutathione reductase activity decreased, as well as malondialdehyde levels. These results suggest that the acute treatment with tibolone prevented the changes in the metabolic parameters analyzed as well as the increase in the levels of malondialdehyde and AGEs induced by ovariectomy and high-fat diet.

SUBACUTE THYROIDITIS WITH ELEVATED SERUM ALKALINE PHOSPHATASE LEVEL

1960 ◽  
Vol XXXIV (II) ◽  
pp. 256-260
Author(s):  
Jörgen Herman Vogt
Keyword(s):  
Alkaline Phosphatase ◽  
Alkaline Phosphatase Level ◽  
Serum Alkaline Phosphatase ◽  
Thyroid Tissue ◽  
Elevated Serum ◽  
Subacute Thyroiditis ◽  
Serum Alkaline Phosphatase Level ◽  
Transient Rise

ABSTRACT A case of subacute thyroiditis is recorded, in which a transient rise in serum alkaline phosphatase values leads to the hypothesis of a transient parathyroid hyper-activity induced by the inflammation of the thyroid tissue in which the parathyroid may be embedded.

Oxidative stress markers in women with polycystic ovary syndrome without insulin resistance

2018 ◽  
Author(s):  
Reveka Gyftaki ◽  
Sofia Gougoura ◽  
Nikolaos Kalogeris ◽  
Vasiliki Loi ◽  
George Koukoulis ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Insulin Resistance ◽  
Polycystic Ovary Syndrome ◽  
Polycystic Ovary ◽  
Oxidative Stress Markers ◽  
Ovary Syndrome ◽  
Stress Markers
Sign in / Sign up

Export Citation Format

Share Document