scholarly journals Preclinical Evaluation of Antiurolithiatic Activity ofViburnum opulusL. on Sodium Oxalate-Induced Urolithiasis Rat Model

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Mert İlhan ◽  
Burçin Ergene ◽  
Ipek Süntar ◽  
Serkan Özbilgin ◽  
Gülçin Saltan Çitoğlu ◽  
...  
Keyword(s):  
Chlorogenic Acid ◽  
Acid Content ◽  
High Performance ◽  
Fruit Juice ◽  
Scientific Evidence ◽  
Folk Medicine ◽  
Sodium Oxalate ◽  
Viburnum Opulus ◽  
Urine And Serum

The aim of the present research is to evaluate the antiurolithiatic effect of the various extracts prepared from the fruits ofViburnum opulusL., in regard to its ethnobotanical record. To induce urolithiasis, 70 mg/kg sodium oxalate was injected to the rats which were housed individually in metabolic cages. The test materials were applied during 7 days. Biochemical (urine and serum parameters), histopathological and antioxidant (TBARs, TSH and GSH) assays were conducted. The urine samples were examined by light microscope for the determination of the calcium oxalate crystals. Lyophilized juice ofV. opulus(LJVO) and lyophilized commercial juice ofV. opulus(LCJVO) exerted potential antiurolithiatic activity which was attributed to its diuretic effect along with the inhibitory action on the oxalate levels and free radical production. We also determined the chlorogenic acid content of the LJVO by high-performance liquid chromatography (HPLC). Chlorogenic acid was determined by using Supelcosil LC-18 (250×4.6 mm, 5 µm) column and acetonitrile: water: 0.2%o-phosphoric acid as a mobile phase. The chlorogenic acid content ofV. opuluswas found to be 0.3227 mg/mL in fruit juice. The results obtained in this study have provided a scientific evidence for the traditional usage ofV. opuluson passing kidney stones in Turkish folk medicine.

Determination of Fungicides in Fruit Juice by Ultrasound-Assisted Dispersive Liquid–Liquid Microextraction Based on Solidification of Floating Organic Solvent Droplets Followed by High Performance Liquid Chromatography

2014 ◽  
Vol 97 (1) ◽  
pp. 183-187 ◽  
Author(s):  
Run-Zhen Fan ◽  
Congyun Liu ◽  
Wenqing Jiang ◽  
Xiaonan Wang ◽  
Fengmao Liu
Keyword(s):  
Organic Solvent ◽  
High Performance ◽  
Fruit Juice ◽  
Enrichment Factors ◽  
Wide Range ◽  
Separation And Preconcentration ◽  
Ultrasound Assisted ◽  
Dispersive Liquid Liquid Microextraction ◽  
Liquid Microextraction

Abstract Ultrasound-assisted dispersive liquid–liquid microextraction (UA-DLLME) based on solidification of the floating organic solvent droplets (SFO) combined with HPLC was used for determination of five fungicides in fruit juice samples. 1-Dodecanol, which has a low density and low toxicity, was used as the extraction solvent in UA-DLLME. The solidification of floating organic dropletsfacilitates the transfer of analytes from the aqueous phase to the organic phase. This method was easy, quick, inexpensive, precise, and linear over a wide range. Under the optimized conditions, the enrichment factors for a 5 mL fruit juice sample were 25 to 56, and the LODs for the five fungicides ranged from 5 to 50 μg/L. The average recoveries ranged from 71.8 to 118.2% with RSDsof 0.9 to 13.9%. Application of the DLLME-SFO technique allows successful separation and preconcentration of the fungicides at a low concentration level in fruit juice samples.

Continuous sample drop flow-microextraction followed by high performance liquid chromatography for determination of triazine herbicides from fruit juices

2017 ◽  
Vol 9 (6) ◽  
pp. 980-985 ◽  
Author(s):  
Toraj Ahmadi-Jouibari ◽  
Yahya Pasdar ◽  
Meghdad Pirsaheb ◽  
Nazir Fattahi
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Fruit Juice ◽  
Environmentally Friendly ◽  
Solvent Free ◽  
Triazine Herbicides ◽  
Drop Flow ◽  
Environmentally Friendly Method

A simple, inexpensive, reliable, dispersive-solvent-free and environmentally friendly method based on CSDF-ME and HPLC-UV was developed for the determination of triazine herbicides in fruit juice samples.

scholarly journals Validation method for determining sodium benzoate in fruit juice drinks in Malang

2019 ◽  
Vol 4 (1) ◽  
pp. 19
Author(s):  
Engrid Juni Astuti ◽  
Roha Fakhri Naufal Ilham ◽  
Januar Rahman
Keyword(s):  
Sodium Benzoate ◽  
Mobile Phase ◽  
High Performance ◽  
Glacial Acetic Acid ◽  
Fruit Juice ◽  
Hplc Method ◽  
Mobile Phase Composition ◽  
Validation Method ◽  
Accuracy And Precision

Sodium benzoate is a preservative that is permitted to be used in food and beverages. The maximum limit for using sodium benzoate in food is 600 mg/L of ingredients calculated as benzoic acid. In this study, High-Performance Liquid Chromatography (HPLC) method using a PDA detector was used for sodium benzoate levels analysis in fruit juice drink circulating in Malang. The mobile phase composition used in this study was methanol pro-HPLC:aqua pro injection (70:30) and added glacial acetic acid to reach pH 3. The analysis was conducted at a wavelength of 245 nm, and the flow rate was set at 1.00 mL/minute. This method was validated against the parameters of selectivity, linearity, accuracy, and precision, and the results obtained to meet the validation requirements. The application of the HPLC method after being validated in the determination of sodium benzoate levels in fruit juice drink circulating in Malang resulted in selectivity Rs ≥ 1.5, linearity obtained in the range 0.1-5 μg/mL with a correlation coefficient (r) = 0,9999, recovery at a concentration of 80%, 100%, and 120% was 105.31%, and precision with an RSD value of 1.40%. Based on the results, it can be concluded that the validation method of analysis shows that the method can be used to determine sodium benzoate level using HPLC with PDA detectors for routine use in fruit juice drink samples.

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