scholarly journals Norfloxacin—Toxicity for Zebrafish (Danio rerio) Focused on Oxidative Stress Parameters

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Marta Bartoskova ◽  
Radka Dobsikova ◽  
Vlasta Stancova ◽  
Ondrej Pana ◽  
Dana Zivna ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Danio Rerio ◽  
Negative Impact ◽  
Toxicity Tests ◽  
Oxygen Species ◽  
Glutathione S Transferase ◽  
Subchronic Toxicity ◽  
Biochemical Processes ◽  
Oxidative Stress Parameters ◽  
Stress Parameters

The aim of the study was to investigate the effects of subchronic exposure of zebrafish (Danio rerio) to a fluoroquinolone norfloxacin, using selected oxidative stress parameters as a target. Toxicity tests were performed on zebrafish according to the OECD Guidelines number 203 and number 215. In the Subchronic Toxicity Test, a significant (P<0.01) increase in the activity of glutathione peroxidase, glutathione S-transferase, and catalase was found. In the test, norfloxacin did not affect lipid peroxidation and catalytic activity of glutathione reductase. From the results, we can conclude that norfloxacin has a negative impact on specific biochemical processes connected with the production of reactive oxygen species in fish tested.

scholarly journals Effects of Desiccation on Metamorphic Climax in Bombina variegata: Changes in Levels and Patterns of Oxidative Stress Parameters

Animals ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 953
Author(s):  
Tamara G. Petrović ◽  
Ana Kijanović ◽  
Nataša Kolarov Kolarov Tomašević ◽  
Jelena P. Gavrić ◽  
Svetlana G. Despotović ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Oxidative Damage ◽  
Antioxidant System ◽  
Water Availability ◽  
System Control ◽  
Glutathione S Transferase ◽  
Oxidative Stress Parameters ◽  
Metamorphic Climax ◽  
Bombina Variegata ◽  
Stress Parameters

In this paper, we examined how the oxidative status (antioxidant system and oxidative damage) of Bombina variegata larvae changed during the metamorphic climax (Gosner stages: 42—beginning, 44—middle and 46—end) and compared the patterns and levels of oxidative stress parameters between individuals developing under constant water availability (control) and those developing under decreasing water availability (desiccation group). Our results revealed that larvae developing under decreasing water availability exhibited increased oxidative damage in the middle and end stages. This was followed by lower levels of glutathione in stages 44 and 46, as well as lower values of catalase, glutathione peroxidase, glutathione S-transferase and sulfhydryl groups in stage 46 (all in relation to control animals). Comparison between stages 42, 44 and 46 within treatments showed that individuals in the last stage demonstrated the highest intensities of lipid oxidative damage in both the control and desiccation groups. As for the parameters of the antioxidant system, control individuals displayed greater variety in response to changes induced by metamorphic climax than individuals exposed to desiccation treatment. The overall decrease in water availability during development led to increased oxidative stress and modifications in the pattern of AOS response to changes induced by metamorphic climax in larvae of B. variegata.

scholarly journals Oxidative stress parameters in juvenile Brazilian flounder Paralichthys orbignyanus (Valenciennes, 1839) (Pleuronectiformes: Paralichthyidae) exposed to cold and heat shocks

2015 ◽  
Vol 13 (3) ◽  
pp. 607-612 ◽  
Author(s):  
Luciano de O. Garcia ◽  
Marcelo H. Okamoto ◽  
Ana Paula K. Riffel ◽  
Etiane M. Saccol ◽  
Maria A. Pavanato ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cold Shock ◽  
Oxygen Species ◽  
Oxidative Stress Parameters ◽  
Seawater Salinity ◽  
Heat Shocks ◽  
Fish Tank ◽  
Gst Activity ◽  
Stress Parameters ◽  
Paralichthys Orbignyanus

The aim of this study was to determine oxidative stress parameters in the liver and gill of Brazilian flounder juveniles (307.0 ± 16.0 g and 30.0 ± 4.0 cm) submitted to different water temperature (17.1, 23.0 and 28.8ºC) for 72 h and maintained at salinity 25‰. After the acclimation of 7 days, in 23ºC, fish were transferred to 200 L tanks containing seawater (salinity 25‰) at 28.8ºC (heat shock), 17.1ºC (cold shock) or 23.0ºC (control), five replicates (five fish tank-1). The sampled collection occurred in 0 (pre-challenge), 3, 24, 48 and 72 h after temperature shock. Flounder exposed to 17.1ºC and 28.8ºC showed significantly higher TBARS levels and GST activity in the liver post-exposition (PE) in relation to the control (23ºC). CAT activity in liver present a significantly increase at 17.1ºC, in first 48 h, and subsequently decrease in 72 h PE in relation to 28.8ºC. The gills of flounder showed significantly higher TBARS levels, GST and CAT activity when submitted at 17.1 and 28.8ºC in relation to 23.0ºC. There were observed changes in lipid peroxidation levels (LPO), CAT and GST activities in the liver and gill of Brazilian flounder in response to reactive oxygen species (ROS) produced by thermal shocks.

scholarly journals The Protective Effect of Lipoic Acid on Selected Cardiovascular Diseases Caused by Age-Related Oxidative Stress

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
Beata Skibska ◽  
Anna Goraca
Keyword(s):  
Oxidative Stress ◽  
Cardiovascular Diseases ◽  
Lipoic Acid ◽  
Aging Process ◽  
Antioxidant Defense ◽  
Ros Production ◽  
Oxygen Species ◽  
Oxidative Stress Parameters ◽  
Age Related ◽  
Stress Parameters

Oxidative stress is considered to be the primary cause of many cardiovascular diseases, including endothelial dysfunction in atherosclerosis and ischemic heart disease, hypertension, and heart failure. Oxidative stress increases during the aging process, resulting in either increased reactive oxygen species (ROS) production or decreased antioxidant defense. The increase in the incidence of cardiovascular disease is directly related to age. Aging is also associated with oxidative stress, which in turn leads to accelerated cellular senescence and organ dysfunction. Antioxidants may help lower the incidence of some pathologies of cardiovascular diseases and have antiaging properties. Lipoic acid (LA) is a natural antioxidant which is believed to have a beneficial effect on oxidative stress parameters in relation to diseases of the cardiovascular system.

scholarly journals Human cultured IMR-32 neuronal-like and U87 glial-like cells have different patterns of toxicity under fluoride exposure

PLoS ONE ◽  
2021 ◽  
Vol 16 (6) ◽  
pp. e0251200
Author(s):  
Bruna Puty ◽  
Leonardo Oliveira Bittencourt ◽  
Iago Cesar Nogueira ◽  
Marília Afonso Rabelo Buzalaf ◽  
Edivaldo Herculano Oliveira ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Water Supply ◽  
Cell Viability ◽  
Human Exposure ◽  
Cell Metabolism ◽  
Oxygen Species ◽  
Oxidative Stress Parameters ◽  
Differential Behavior ◽  
Stress Parameters

Background Fluoride (F) is a naturally exists in nature but several studies have indicated it as an environmental toxicant to all leaving beings. Human F exposure has increased over the years since this ion has been used by industry on foods, beverages, toothpastes and on water supply. Although F is safe at optimal concentrations in water supply, human exposure to high levels could trigger neurofunctional deficits. Materials and methods In this study, human glial-like (U87) and neuronal-like (IMR-32) cells lineages were used to access F toxicity and CNS cell sensibility on both cell facing the same protocol. Cells were exposed to F over 3, 5 and 10 days on two different F concentrations. Fluoride exposed cells were evaluated by standard toxicity assays to cell viability, apoptosis, necrosis and general cell metabolism. Oxidative stress parameters were evaluated by ATP and ROS levels, lipid peroxidation, GSH/GSSG ratio and comet assay. Results No changes were observed in IMR-32 at any given time while after 10 days of exposure to 0.22μg/mL, U87 glial-like cells showed signs of toxicity such as decreased cell viability by necrosis while general cell metabolism was increased. Oxidative stress parameters were next evaluated only on U87 glial-like cells after 10 days of exposure. F induced a decrease on ATP levels while no changes were observed on reactive oxygen species and lipid peroxidation. GSH/GSSG ratio was decreased followed by DNA damage both on 0.22μg/mL F. Conclusions Our results suggest an important differential behavior of the distinct types of cells exposed to the different fluoride concentrations, pointing that the U87 glial-like cells as more susceptible to damage triggered by this ion.

Does human myocardium show a gender dependent modification of oxidative stress parameters?

2014 ◽  
Vol 62 (S 01) ◽  
Author(s):  
K. Buschmann ◽  
Y. Gramlich ◽  
M. Oelze ◽  
A. Daiber ◽  
T. Münzel ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Human Myocardium ◽  
Oxidative Stress Parameters ◽  
Dependent Modification ◽  
Stress Parameters

Evaluation of oxidative stress parameters in subacute thyroiditis patients

2019 ◽  
Author(s):  
Murat Dağdeviren ◽  
Arzu Or Koca ◽  
Tolga Akkan ◽  
İhsan Ateş ◽  
Salim Neşelioğlu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Subacute Thyroiditis ◽  
Oxidative Stress Parameters ◽  
Stress Parameters
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