scholarly journals Systemic Treatment with Strontium Ranelate Accelerates the Filling of a Bone Defect and Improves the Material Level Properties of the Healing Bone

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Giovanna Zacchetti ◽  
Romain Dayer ◽  
René Rizzoli ◽  
Patrick Ammann
Keyword(s):  
Trabecular Bone ◽  
Cortical Bone ◽  
Bone Defect ◽  
Strontium Ranelate ◽  
Bone Microarchitecture ◽  
Female Rats ◽  
Micro Computed Tomography ◽  
Ct Analysis ◽  
Defect Filling

Rapid bone defect filling with normal bone is a challenge in orthopaedics and dentistry. Strontium ranelate (SrRan) has been shown to in vitro decrease bone resorption and increase bone formation, and represents a potential agent with the capacity to accelerate bone defect filling. In this study, bone tibial defects of 2.5 mm in diameter were created in 6-month-old female rats orally fed SrRan (625 mg/kg/d; 5/7 days) or vehicle for 4, 8, or 12 weeks (10 rats per group per time point) from the time of surgery. Tibias were removed. Micro-architecture was determined by micro-computed tomography (µCT) and material level properties by nanoindentation analysis.µCT analysis showed that SrRan administration significantly improved microarchitecture of trabecular bone growing into the defect after 8 and 12 weeks of treatment compared to vehicle. SrRan treatment also accelerated the growth of cortical bone over the defect, but with different kinetics compared to trabecular bone, as the effects were already significant after 4 weeks. Nanoindentation analysis demonstrated that SrRan treatment significantly increased material level properties of both trabecular bone and cortical bone filling the defect compared to vehicle. SrRan accelerates the filling of bone defect by improving cortical and trabecular bone microarchitecture both quantitatively and qualitatively.

scholarly journals Strontium Ranelate Effect on the Repair of Bone Defects and Molecular Components of the Cortical Bone of Rats

2016 ◽  
Vol 27 (5) ◽  
pp. 502-507 ◽  
Author(s):  
Jucely Aparecida da Rosa ◽  
◽  
Kumiko Koibuchi Sakane ◽  
Karina Cecília Panelli Santos ◽  
Vivian Bradaschia Corrêa ◽  
...  
Keyword(s):  
Cortical Bone ◽  
Bone Defect ◽  
Strontium Ranelate ◽  
Bone Defects ◽  
Female Rats ◽  
Trabecular Thickness ◽  
Computed Microtomography ◽  
Group A ◽  
Molecular Components ◽  
Made In

Abstract This study was conducted to evaluate the effects of treatment with strontium ranelate (SR) on the repair of bone defects and molecular components of bones in femurs. Adult female rats (n=27) were subjected to ovariectomy (OVX) or Sham surgery. Thirty days after surgery, a defect was made in the femur and the animals were then divided into three groups: OVX, SHAM and OVX+SR. Euthanasia was performed four weeks after the bone defect surgery. Repair in bone defect was assessed by computed microtomography (μCT) and chemical composition of cortical bone was analyzed by Fourier transform infrared (FTIR) spectroscopy and energy dispersive X-ray spectroscopy (EDS). The trabecular thickness (Tb.Th) of the newly formed bone in the OVX+SR group was significantly higher than that for the OVX group. The collagen maturity in the OVX+SR group was smaller than in the other two groups. In this group, a significant increase in the amount of strontium (Sr) and a decrease in the amount of calcium (Ca) embedded to bone tissue were also observed. Systemic treatment with SR improved microarchitecture of the newly formed bone inside the defect, but decreased cross-linking of mature collagen in cortical bone.

scholarly journals A Comparison of Micro-CT and Dental CT in Assessing Cortical Bone Morphology and Trabecular Bone Microarchitecture

PLoS ONE ◽  
2014 ◽  
Vol 9 (9) ◽  
pp. e107545 ◽  
Author(s):  
Jui-Ting Hsu ◽  
Ying-Ju Chen ◽  
Jung-Ting Ho ◽  
Heng-Li Huang ◽  
Shun-Ping Wang ◽  
...  
Keyword(s):  
Trabecular Bone ◽  
Cortical Bone ◽  
Bone Microarchitecture ◽  
Micro Ct ◽  
Bone Morphology ◽  
Trabecular Bone Microarchitecture ◽  
Dental Ct

Characterization of Human Cortical and Trabecular Bone Structural Change by NMR and Micro-CT

2012 ◽  
Author(s):  
Qingwen Ni ◽  
Todd Bredbenner
Keyword(s):  
Trabecular Bone ◽  
Spin Relaxation ◽  
Structural Changes ◽  
Relaxation Times ◽  
Proton Nuclear Magnetic Resonance ◽  
Micro Ct ◽  
Micro Computed Tomography ◽  
T2 Relaxation ◽  
Ct Measurement

The techniques of low-field pulsed proton nuclear magnetic resonance (NMR) spin relaxation and Micro-CT are described for assessment of structural changes of human cortical and trabecular bone in vitro. The technique involves spin-spin relaxation measurement and inversion spin-spin spectral analysis methods for NMR. From NMR measurement, the CPMG T2 relaxation data can be inverted to T2 relaxation distribution and this distribution then can be transformed to a pore size distribution with the longer relaxation times corresponding to larger pores. In Micro-CT measurement, each trabecular bone specimen was individually scanned using a micro-computed tomography (micro-CT) system and 12 micron dimensionally-isotropic voxels were reconstructed. Due to the resolution limitation, Micro-CT measurement on cortical bone was not success, however, NMR measurement was success for both cortical and trabecular bones.

scholarly journals Bone microarchitecture and estimated bone strength in men with active acromegaly

2017 ◽  
Vol 177 (5) ◽  
pp. 409-420 ◽  
Author(s):  
Paula P B Silva ◽  
Fatemeh G Amlashi ◽  
Elaine W Yu ◽  
Karen J Pulaski-Liebert ◽  
Anu V Gerweck ◽  
...  
Keyword(s):  
Bone Density ◽  
Trabecular Bone ◽  
Cortical Bone ◽  
Bone Strength ◽  
Bone Microarchitecture ◽  
Healthy Controls ◽  
Trabecular Bone Density ◽  
Trabecular Bone Microarchitecture ◽  
Skeletal Site ◽  
Active Acromegaly

Context Both acromegaly and adult growth hormone deficiency (GHD) are associated with increased fracture risk. Sufficient data are lacking regarding cortical bone microarchitecture and bone strength, as assessed by microfinite element analysis (µFEA). Objective To elucidate both cortical and trabecular bone microarchitecture and estimated bone strength in men with active acromegaly or GHD compared to healthy controls. Design and subjects Cross-sectional study at a clinical research center, including 48 men (16 with acromegaly, 16 with GHD and 16 healthy controls). Outcome measures Areal bone mineral density (aBMD), cortical and trabecular bone microarchitecture and estimated bone strength (µFEA) at the radius and tibia. Results aBMD was not different between the 3 groups at any skeletal site. At the radius, patients with acromegaly had greater cortical area (P < 0.0001), cortical thickness (P = 0.0038), cortical pore volume (P < 0.0001) and cortical porosity (P = 0.0008), but lower trabecular bone density (P = 0.0010) compared to controls. At the tibia, patients with acromegaly had lower trabecular bone density (P = 0.0082), but no differences in cortical bone microstructure. Compressive strength and failure load did not significantly differ between groups. These findings persisted after excluding patients with hypogonadism. Bone microarchitecture was not deficient in patients with GHD. Conclusions Both cortical and trabecular microarchitecture are altered in men with acromegaly. Our data indicate that GH excess is associated with distinct effects in cortical vs trabecular bone compartments. Our observations also affirm the limitations of aBMD testing in the evaluation of patients with acromegaly.

scholarly journals Deciphering an extreme morphology: bone microarchitecture of the hero shrew backbone (Soricidae: Scutisorex )

2020 ◽  
Vol 287 (1926) ◽  
pp. 20200457 ◽  
Author(s):  
Stephanie M. Smith ◽  
Kenneth D. Angielczyk
Keyword(s):  
Trabecular Bone ◽  
Sagittal Plane ◽  
Bone Microarchitecture ◽  
Compressive Load ◽  
Bone Architecture ◽  
Micro Computed Tomography ◽  
Vertebral Centra ◽  
Behavioural Patterns ◽  
Compressive Loads

Biological structures with extreme morphologies are puzzling because they often lack obvious functions and stymie comparisons to homologous or analogous features with more typical shapes. An example of such an extreme morphotype is the uniquely modified vertebral column of the hero shrew Scutisorex , which features numerous accessory intervertebral articulations and massively expanded transverse processes. The function of these vertebral structures is unknown, and it is difficult to meaningfully compare them to vertebrae from animals with known behavioural patterns and spinal adaptations. Here, we use trabecular bone architecture of vertebral centra and quantitative external vertebral morphology to elucidate the forces that may act on the spine of Scutisorex and that of another large shrew with unmodified vertebrae ( Crocidura goliath ). X-ray micro-computed tomography (µCT) scans of thoracolumbar columns show that Scutisorex thori is structurally intermediate between C. goliath and S. somereni internally and externally, and both Scutisorex species exhibit trabecular bone characteristics indicative of higher in vivo axial compressive loads than C. goliath. Under compressive load, Scutisorex vertebral morphology is adapted to largely restrict bending to the sagittal plane (flexion). Although these findings do not solve the mystery of how Scutisorex uses its byzantine spine in vivo , our work suggests potentially fruitful new avenues of investigation for learning more about the function of this perplexing structure.

scholarly journals Formononetin, a methoxy isoflavone, enhances bone regeneration in a mouse model of cortical bone defect

2017 ◽  
Vol 117 (11) ◽  
pp. 1511-1522 ◽  
Author(s):  
Krishna Bhan Singh ◽  
Manisha Dixit ◽  
Kapil Dev ◽  
Rakesh Maurya ◽  
Divya Singh
Keyword(s):  
Bone Regeneration ◽  
Cortical Bone ◽  
Bone Defect ◽  
Bone Healing ◽  
Drill Hole ◽  
Repair Process ◽  
Fracture Repair ◽  
Injury Site ◽  
Healing Effect ◽  
Ct Analysis

AbstractThe bone regeneration and healing effect of formononetin was evaluated in a cortical bone defect model that predominantly heals by intramembranous ossification. For this study, female Balb/c mice were ovariectomised (OVx) and a drill-hole injury was generated in the midfemoral bones of all animals. Treatment with formononetin commenced the day after and continued for 21 d. Parathyroid hormone (PTH1–34) was used as a reference standard. Animals were killed at days 10 and 21. Femur bones were collected at the injury site for histomorphometry studies using microcomputed tomography (μCT) and confocal microscopy. RNA and protein were harvested from the region surrounding the drill-hole injury. For immunohistochemistry, 5 µm sections of decalcified femur bone adjoining the drill-hole site were cut.μCT analysis showed that formononetin promoted bone healing at days 10 and 21 and the healing effect observed was significantly better than in Ovx mice and equal to PTH treatment in many aspects. Formononetin also significantly enhanced bone regeneration as assessed by calcein-labelling studies. In addition, formononetin enhanced the expression of osteogenic markers at the injury site in a manner similar to PTH. Formononetin treatment also led to predominant runt-related transcription factor 2 and osteocalcin localisation at the injury site. These results support the potential of formononetin to be a bone-healing agent and are suggestive of its promising role in the fracture-repair process.

Contribution of Three-Dimensional Trabecular Bone Microstructure of the Proximal Femur to its Mechanical Properties as Assessed by Micro-Finite Element Analysis

2006 ◽  
Vol 321-323 ◽  
pp. 278-281
Author(s):  
Wen Quan Cui ◽  
Ye Yeon Won ◽  
Myong Hyun Baek ◽  
Kwang Kyun Kim
Keyword(s):  
Finite Element Analysis ◽  
Finite Element ◽  
Trabecular Bone ◽  
Young’S Modulus ◽  
Young's Modulus ◽  
Bone Microarchitecture ◽  
Element Model ◽  
Micro Ct ◽  
Micro Computed Tomography ◽  
Element Analysis

The purpose of this study was to investigate the contribution of the microstructural properties of trabecular bone in predicting its elastic modulus in the intertrochanteric region. A total of 15 trabecular bone core specimens were obtained from the proximal femurs of patients undergoing total hip arthroplasty. The micro-computed tomography (micro-CT) was used to scan each specimen to obtain micro-morphology. Microstructural parameters were directly calculated using software. Micro-CT images were converted to micro-finite element model using meshing technique, and then micro-finite element analysis (FEA) was performed to assess the mechanical property (Young’s modulus) of trabecular bone. The results showed that the ability to explain this variance of Young’s modulus is improved by combining the structural indices with each other. It suggested that assessment of bone microarchitecture should be added as regards detection of osteoporosis and evaluation of the efficacy of drug treatments for osteoporosis.

Three-Dimensional Local Measurements of Bone Strain and Displacement: Comparison of Three Digital Volume Correlation Approaches

2015 ◽  
Vol 137 (7) ◽  
Author(s):  
Marco Palanca ◽  
Gianluca Tozzi ◽  
Luca Cristofolini ◽  
Marco Viceconti ◽  
Enrico Dall'Ara
Keyword(s):  
Trabecular Bone ◽  
Cortical Bone ◽  
Computational Cost ◽  
Three Dimensional ◽  
Digital Volume Correlation ◽  
Micro Computed Tomography ◽  
Local Approach ◽  
Accuracy And Precision ◽  
Local Measurements ◽  
The Difference

Different digital volume correlation (DVC) approaches are currently available or under development for bone tissue micromechanics. The aim of this study was to compare accuracy and precision errors of three DVC approaches for a particular three-dimensional (3D) zero-strain condition. Trabecular and cortical bone specimens were repeatedly scanned with a micro-computed tomography (CT). The errors affecting computed displacements and strains were extracted for a known virtual translation, as well as for repeated scans. Three DVC strategies were tested: two local approaches, based on fast-Fourier-transform (DaVis-FFT) or direct-correlation (DaVis-DC), and a global approach based on elastic registration and a finite element (FE) solver (ShIRT-FE). Different computation subvolume sizes were tested. Much larger errors were found for the repeated scans than for the virtual translation test. For each algorithm, errors decreased asymptotically for larger subvolume sizes in the range explored. Considering this particular set of images, ShIRT-FE showed an overall better accuracy and precision (a few hundreds microstrain for a subvolume of 50 voxels). When the largest subvolume (50–52 voxels) was applied to cortical bone, the accuracy error obtained for repeated scans with ShIRT-FE was approximately half of that for the best local approach (DaVis-DC). The difference was lower (250 microstrain) in the case of trabecular bone. In terms of precision, the errors shown by DaVis-DC were closer to the ones computed by ShIRT-FE (differences of 131 microstrain and 157 microstrain for cortical and trabecular bone, respectively). The multipass computation available for DaVis software improved the accuracy and precision only for the DaVis-FFT in the virtual translation, particularly for trabecular bone. The better accuracy and precision of ShIRT-FE, followed by DaVis-DC, were obtained with a higher computational cost when compared to DaVis-FFT. The results underline the importance of performing a quantitative comparison of DVC methods on the same set of samples by using also repeated scans, other than virtual translation tests only. ShIRT-FE provides the most accurate and precise results for this set of images. However, both DaVis approaches show reasonable results for large nodal spacing, particularly for trabecular bone. Finally, this study highlights the importance of using sufficiently large subvolumes, in order to achieve better accuracy and precision.

Sign in / Sign up

Export Citation Format

Share Document