scholarly journals Application of an Optimized HPLC Method for the Detection of Various Phenolic Compounds in Apples from Lithuanian Cultivars

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Mindaugas Liaudanskas ◽  
Pranas Viškelis ◽  
Valdas Jakštas ◽  
Raimondas Raudonis ◽  
Darius Kviklys ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Chlorogenic Acid ◽  
Mobile Phase ◽  
Analytical Procedure ◽  
Total Content ◽  
Hplc Method ◽  
Quercetin Derivatives ◽  
Phenol Extraction ◽  
Hplc Mobile Phase ◽  
Ultrasound Bath

A specific analytical procedure including sample preparation and HPLC analysis was developed and validated for the detection of phenolic compounds in the samples of different apples from popular Lithuanian cultivars “Aldas,” “Auksis,” “Ligol,” and “Šampion.” The conditions for phenol extraction were optimized: the solvent of the extraction was 70% (v/v) ethanol, and the extraction was performed in an ultrasound bath for 20 min at the temperature of 40°C. The HPLC mobile phase consisted of 2% (v/v) acetic acid in water and 100% (v/v) acetonitrile. Using the HPLC technique, 11 analytes were identified, and their specificity was confirmed: procyanidin B1, (+)-catechin, chlorogenic acid, procyanidin B2, (−)-epicatechin, rutin, hyperoside, isoquercitrin, avicularin, quercitrin, and phloridzin. Chlorogenic acid was the major component in “Aldas,” “Auksis,” and “Ligol” and procyanidin B2 in “Šampion.” Hyperoside and avicularin were the dominant compounds of all the identified quercetin derivatives in “Aldas” and “Auksis;” hyperoside in “Šampion;” and quercitrin in “Ligol.” The total content of phenolic compounds varied from 1641.0 ± 47.9 μg/g (cv. “Ligol”) to 4291.3 ± 154.2 μg/g (cv. “Aldas”).

scholarly journals Determination of the Phenolic Composition and Antioxidant Activity of Pear Extracts

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Mindaugas Liaudanskas ◽  
Kristina Zymonė ◽  
Jonas Viškelis ◽  
Almantas Klevinskas ◽  
Valdimaras Janulis
Keyword(s):  
Phenolic Compounds ◽  
Chlorogenic Acid ◽  
Mobile Phase ◽  
Analytical Procedure ◽  
Total Content ◽  
Hplc Method ◽  
Phenolic Composition ◽  
Intermediate Precision ◽  
Hplc Mobile Phase

The specific HPLC analytical procedure was developed and validated for the determination of phenolic compounds in pear samples of different popular cultivars “Conference,” “Concordia,” “Grabova,” and “Patten.” HPLC mobile phase consisted of 0.05% (v/v) trifluoroacetic acid in water and 100% (v/v) acetonitrile. The HPLC method was used to identify and confirm the specificity of 8 analytes: chlorogenic acid, rutin, hyperoside, isoquercitrin, isorhamnetin rutinoside, quercitrin, quercitrin malonyl glucoside, and isorhamnetin glucoside. Repeatability % RSD did not exceed 3.87%, and intermediate precision did not exceed 4.63%. The total content of phenolic compounds varied from0.51±0.001 mg/g (cv. “Concordia”) to1.11±0.013 mg/g (cv. “Patten”). Chlorogenic acid was the major component in all the tested pear cultivars. The highest amount of chlorogenic acid (0.69±0.033 mg/g) was found in pear samples of the cultivar “Grabova,” and the highest amount of flavonol compounds (1.11±0.013 mg/g) was found in pear samples of the cultivar “Concordia.”

scholarly journals Variation in the Contents of Neochlorogenic Acid, Chlorogenic Acid and Three Quercetin Glycosides in Leaves and Fruits of Rowan (Sorbus) Species and Varieties from Collections in Lithuania

2013 ◽  
Vol 8 (8) ◽  
pp. 1934578X1300800 ◽  
Author(s):  
Kristina Gaivelyte ◽  
Valdas Jakstas ◽  
Almantas Razukas ◽  
Valdimaras Janulis
Keyword(s):  
Phenolic Compounds ◽  
Chlorogenic Acid ◽  
Quantitative Estimation ◽  
Total Content ◽  
Hplc Method ◽  
Raw Material ◽  
Quantitative Composition ◽  
Fruit Samples ◽  
Neochlorogenic Acid ◽  
Quercetin Glycosides

Our research aim was to investigate the quantitative composition of neochlorogenic acid, chlorogenic acid, rutin, hyperoside and isoquercitrin in leaves and fruit of introduced rowan plants to Lithuania using a HPLC method. In leaf and fruit samples, quantitative estimation of neochlorogenic acid (in leaf samples 1.11–11.49 mg/g, in fruit samples 0.93–5.43 mg/g), chlorogenic acid (in leaf samples 2.74–21.91 mg/g, in fruit samples 0.55–7.50 mg/g), rutin (in leaf samples 0.03–4.88 mg/g, in fruit samples 0.02-0.39 mg/g), hyperoside (in leaf samples 0.15–7.44 mg/g, in fruit samples 0.02–1.19 mg/g) and isoquercitrin (in leaf samples 0.75–5.24 mg/g, in fruit samples 0.02-0.65 mg/g) was performed. In leaf samples, the total content of identified phenolic compounds ranged from 7.18 to 35.74 mg/g. In fruit, raw material total content of neochlorogenic acid, chlorogenic acid and identified flavonoids varied from 2.24 to 11.19 mg/g. Comparison of raw material samples of Sorbus species according to their quantitative composition of identified phenolic compounds using cluster analysis singularized S. commixta leaf and fruit samples. These contained the highest contents of chlorogenic acid, hyperoside and isoquercitrin, but also the lowest contents of neochlorogenic acid and rutin. The highest contents of neochlorogenic acid were recorded for S. lancifolia leaves, and of rutin for S. anglica leaves.

scholarly journals HPLC study of phenolic compounds in Mirabilis jalapa raw material

Pharmacia ◽  
2020 ◽  
Vol 67 (3) ◽  
pp. 145-152
Author(s):  
Dhurgham Khalid Abed Sarray ◽  
Liliia M. Horiacha ◽  
Iryna O. Zhuravel ◽  
Andrii I. Fedosov
Keyword(s):  
Phenolic Compounds ◽  
Folk Medicine ◽  
Total Content ◽  
Hplc Method ◽  
Raw Material ◽  
Control Methods ◽  
Quantitative Content ◽  
Different Types ◽  
Mirabilis Jalapa ◽  
Hplc Study

Mirabilis jalapa is a popular decorative plant valued for its beautiful multicolored flowers. Folk medicine in various countries applies Mirabilis jalapa as anti-microbial, anti-inflammatory, diuretic, spasmolytic drug. Chemical composition of different types of Mirabilis jalapa has not yet been adequately studied which is an obstacle for its application in medicine. The qualitative composition and quantitative content of phenolic compounds were studied by the HPLC method. The performed experiment revealed presence of hydroxycinnamic acids, flavonoids, isoflavonoids and coumarins in tested herb. The content of phenolic compounds was the highest in Mirabilis jalapa flowers, counting as much as 2977.41 ± 59.55 µg/mg. Total content of phenolic compounds in Mirabilis jalapa herb was 304.25 ± 6.08 µg/mg, in fruits – 67.92 ± 1.36 µg/mg, and in roots – 12.44 ± 0.25 µg/mg. Quantitatively neochlorogenic acid dominated in flowers, chlorogenic acid in fruits, whereas Mirabilis jalapa herb mostly contained rutin and hyperoside. The obtained results will be useful in the development of quality control methods for Mirabilis jalapa herb and manufacture of drug preparations on its basis.

scholarly journals Validation of an HPLC method for the simultaneous determination of eletriptan and UK 120.413

2006 ◽  
Vol 71 (11) ◽  
pp. 1195-1205 ◽  
Author(s):  
Mira Zecevic ◽  
Biljana Jocic ◽  
Snezana Agatonovic-Kustrin ◽  
Ljiljana Zivanovic
Keyword(s):  
Mobile Phase ◽  
Analytical Procedure ◽  
Internal Standard ◽  
Correlation Coefficients ◽  
Hplc Method ◽  
Control Analysis ◽  
Internal Standard Method ◽  
Rp Hplc ◽  
Good Repeatability

Arapid and sensitive RPHPLCmethod was developed for the routine control analysis of eletriptan hydrobromide and its organic impurity UK 120.413 in Relpax? tablets. The chromatography was performed at 20?C using a C18 XTerra ? (5 ?m, 150 x 4,6 mm) column at a flow rate 1.0 ml/min. The drug and its impurity were detected at 225 nm. The mobile phase consisted of TEA (1 %) - methanol (67.2:32.8 v/v), the pH of which was adjusted to 6.8 with 85 % orthophosphoric acid. Quantification was accomplished by the internal standard method. The developed RP HPLC method was validated by testing: accuracy, precision, repeatability, specificity, detection limit, quantification limit, linearity, robustness and sensitivity. High linearity of the analytical procedure was confirmed over the concentration range of 0.05 - 1.00 mg/ml for eletriptan hydrobromide and from 0.10 - 1.50 ?g/ml for UK 120.413, with correlation coefficients greater than r = 0.995. The low value of the RSD expressed the good repeatability and precision of the method. Experimental design and a response surface method were used to test robustness of the analytical procedure and to evaluate the effect of variation of the method parameters, namely the mobile phase composition, pH and temperature. They showed small deviations from the method setting. The good recovery and low RSD confirm the suitability of the proposed RP HPLC method for the routine determination of eletriptan hydrobromide and its impurity UK 120.413 in Relpax? tables.

scholarly journals Determination of Individual Homologues and Total Content of Benzalkonium Chloride by Reversed-Phase High-Performance Liquid Chromatography Using a Short Butyl Column

2009 ◽  
Vol 92 (6) ◽  
pp. 1644-1651 ◽  
Author(s):  
Fangzhu Liu ◽  
Kang Ping Xiao ◽  
Abu M Rustum
Keyword(s):  
Benzalkonium Chloride ◽  
Mobile Phase ◽  
High Performance ◽  
Total Concentration ◽  
Total Content ◽  
Reversed Phase ◽  
Hplc Method ◽  
Raw Material ◽  
Short Column

Abstract Benzalkonium chloride (a mixture of alkylbenzyldimethylammonium chlorides that usually contains C-10, C-12, C-14, and C-16 homologues), commonly known as BKC, is used as a bacteriostatic agent in many household, food, and drug products. In this paper, we report a simple, rapid, robust, and stability-indicating reversed-phase HPLC method using a short butyl (C4) column for the simultaneous determination of each individual homologue content, as well as the total concentration of individual homologues in commercial bulk raw material batches of BKC samples. The chromatographic separation was performed on a 5 cm ACE C4 column with mobile phase consisting of water, acetonitrile, and potassium chloride. Even though using a short column can potentially cause some challenges to resolving certain critical pairs of peaks, we have successfully separated all of the analyte peaks (including those from stressed, degraded products) on a short column using an optimal mobile phase.

scholarly journals Content of Phenolic Compounds and Antioxidant Properties in Seeds of Sweet and Bitter Cultivars of Lupine (Lupinus angustifolius)

2018 ◽  
Vol 13 (10) ◽  
pp. 1934578X1801301 ◽  
Author(s):  
Angelika Król ◽  
Ryszard Amarowicz ◽  
Stanisław Weidner
Keyword(s):  
Phenolic Compounds ◽  
Phenolic Acids ◽  
Colorimetric Method ◽  
Antioxidant Properties ◽  
Reducing Power ◽  
Total Content ◽  
Hplc Method ◽  
Lupinus Angustifolius ◽  
Cation Radicals ◽  
Narrow Leaf

The main purpose was to demonstrate differences in the total content of phenolic compounds, phenolic acids and to analysis differences in the antioxidant properties of extracts obtained from seeds of several narrow-leaf lupine cultivars ( Lupinus angustifolius L.). The biological material consisted of seeds of four cultivars of narrow-leaf lupine characterized by different concentrations of alkaloids. The total content of phenolics was determined with the colorimetric method using the Folin-Ciocalteau reagent. Other determinations included the ability of seed extracts to scavenge DPPH• radicals and ABTS•+ cation radicals. The reducing power of the extracts was also determined. Chromatographic and qualitative separation of the phenolic compounds present in seeds was performed with the RP-HPLC method. Out of the four cultivars, the bitter narrow-leaf lupine produced seeds with the highest total content of phenolic compounds, which was also correlated with its antiradical characteristics. In the analyzed seeds were also found to contain three phenolic acids: ferulic, sinapinic and p-coumaric one.

scholarly journals Фенольні сполуки листя Sorbus domestіca і Sorbus graeca

2018 ◽  
Author(s):  
O. V. Krivoruchko
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Phenolic Compounds ◽  
Chlorogenic Acid ◽  
High Performance ◽  
Hplc Method ◽  
Chromatography Method ◽  
Pharmacological Studies ◽  
Rosaceae Family ◽  
Liquid Chromatography Method

Introduction. Sorbus domestіca and Sorbus graeca from the Rosaceae family are cultivated in Ukraine in gardens and parks. Their chemical composition is investigated insufficiently.The aim of the study – to research the composition and the content of phenolic compounds of leaves of Sorbus domestіca and Sorbus graeca.Research Methods. The content of phenolic compounds in leaves of Sorbus domestіca and Sorbus graeca was carried out by the high performance liquid chromatography method on Agilent Technologies chromatograph.Results and Discussion. In leaves of Sorbus domestіca chlorogenic acid, rutin, quercetin-3-O-glucoside and quercetin were identified; in leaves of Sorbus graeca chlorogenic and neochlorogenic acids, rutin and 4'-methoxyquercetin-3-O-sophoroside were identified. The content of hydroxycinnamic acids in leaves of Sorbus domestіca is 389 mg/100 g, in leaves of Sorbus graeca – 147 mg/100 g (in terms of chlorogenic acid). The content of flavonoids in leaves of Sorbus domestіca is 1888 mg/100 g, in leaves of Sorbus graeca – 727 mg/100 g (in terms of rutin).Conclusions. The composition and the content of phenolic compounds of Sorbus domestіca and Sorbus graeca were studied by the HPLC method. Leaves of Sorbus domestіca are more perspective for further pharmacological studies.

scholarly journals COMPONENT COMPOSITION OF PHENOLIC COMPOUNDS OF SEVEN SAUSSUREA SPECIES

2018 ◽  
pp. 197-204
Author(s):  
Елена (Elena) Юрьевна (Yur'yevna) Авдеева (Avdeeva) ◽  
Лариса (Larisa) Николаевна (Nikolayevna) Зибарева (Zibareva) ◽  
Евгения (Evgeniya) Александровна (Aleksandrovna) Кастерова (Kasterova) ◽  
Ярослав (Yaroslav) Евгеньевич (Yevgen'yevich) Решетов (Reshetov) ◽  
Маргарита (Margarita) Николаевна (Nikolayevna) Шурупова (Shurupova) ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Chlorogenic Acid ◽  
Carboxylic Acids ◽  
Component Composition ◽  
Hplc Method ◽  
First Time ◽  
Salicylic Acids

Using HPLC method was studied the component composition of phenolic compounds of the fractions from extracts of the seven Saussurea DC species, grown in Siberia: S. controversa DC, S. latifolia Ledeb., S. parviflora (Poir.) DC, S. frolovii Ledeb., S. amara (L.) DC, S. salicifolia DC, S. daurica Adams. In samples S. amara, S. frolowii, S. daurica, S. salicifolia, S. latifolia, S. controversa a significant amount of syringin (up to 25.0 mg/g of extract) was found. Phenol carboxylic acids are chlorogenic, caffeic, ferulic, syringic, oxybenzoic and salicylic acids with a predominance of chlorogenic acid (up to 53.3 mg/g). Isocvercitrine is identified in S. parviflora, S. amara, S. salicifolia and S. frolowii, cynaroside in S. daurica and S. frolowii, hyperoside in S. salicifolia and S. parviflora; rutin in S. amara, S. controversa and S. parviflora. In S. salicifolia ethylacetate fractions avicularin was found for the first time. The content of flavonoids in seven Saussurea species from 28.1 to 133.2 mg/g of extract was changed.

Analisis Kadar Zat Pewarna Tartrazin pada Minuman Ringan Berkarbonasi Khas Pekalongan dengan Metode High Performance Liquid Chromatography (HPLC)

2021 ◽  
Vol 1 ◽  
pp. 685-691
Author(s):  
AT Meta Lansamigi ◽  
Achmad Vandian Nur ◽  
W Wirasti ◽  
Khusna Santika Rahmasari
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Phenolic Compounds ◽  
Mobile Phase ◽  
High Performance ◽  
Hplc Method ◽  
Lemon Yellow ◽  
Colour Reaction ◽  
Auc Value ◽  
Carbonated Drinks

AbstractTartrazine is a coloring agent which is a mixture of phenolic compounds, polycyclic hydrocarbons, and heterocyclics. Because it dissolves easily in water, tartrazine is commonly used as a coloring agent in beverages. The addition of tartrazine in the drink aims to give it a more attractive color, namely lemon yellow. The purpose of this study was to analyze the tartrazine content in carbonated drinks and to determine whether the tartrazine content in the sample was in accordance with the BPOM Regulation No. 11 of 2019. Qualitative testing using colour reaction with FeSO4. Quantitative testing using the high performance liquid chromatography (HPLC) method with a mobile phase of acetonitrile, methanol and aquabides with a ratio (65,5 : 23,5 : 11). The data obtained is the AUC value. Data analisys using linear regression. The resul of qualitatif analysis with acolor test using FeSO4 reagent which produces a color until it is cloudy and is present on the surface. While the quanitative analysis using HPLC obtained the average levels in the oranfe sample 1,109 mg/kg and pineapple sample 0,933 mg/kg.Key words: Analysis, levels, tartrazine, dye, HPLC AbstrakTartrazin merupakan zat pewarna campuran dari senyawa fenol, hidrokarbon polisiklik, dan heterosiklik. Pada umumnya tartrazin digunakan sebagai bahan pewarna minuman karena mudah larut dalam air. Penambahan tartrazin dalam minuman bertujuan untuk memberikan warna yang lebih menarik yaitu kuning lemon. Tujuan dari penelitian ini adalah untuk menganalisis kandungan tartrazin dalam minuman berkarbonasi dan untuk mengetahui apakah kadar tartrazin dalam sampel sudah sesuai dengan peraturan BPOM No.11 Tahun 2019. Pengujian secara kualitatif dengan metode uji warna menggunakan FeSO4. Pengujian secara kuantitatif menggunakan metode High Performance Liquid Chromatography (HPLC) dengan fase gerak asetonitril, metanol, dan aquabides dengan perbandingan (65,5 : 23,5 : 11). Data yang didapat adalah nilai AUC. Analisis data menggunakan regresi linier. Hasil dari analisis kualitataif dengan uji warna menggunakan reagen FeSO4 yang menghasilkan warna sampel keruh dan terdapat endapan. Sedangkan pada analisis kuantitif menggunakan HPLC didapatkan rata-rata kadar pada sampel rasa jeruk 1,109 mg/kg dan pada sampel nanas 0,933 mg/kg.Kata kunci : Analisis, kadar, tartrazin, pewarna, HPLC

scholarly journals Identification and Quantification of Phenolic Compounds from Red Currant (Ribes rubrum L.) and Raspberries (Rubus idaeus L.)

2017 ◽  
Vol 6 ◽  
pp. 30-37 ◽  
Author(s):  
Adina Frum ◽  
Cecilia Georgescu ◽  
Felicia Gabriela Gligor ◽  
Carmen Dobrea ◽  
Ovidiu Tița
Keyword(s):  
Phenolic Compounds ◽  
Ferulic Acid ◽  
Chlorogenic Acid ◽  
Gallic Acid ◽  
Cinnamic Acid ◽  
Syringic Acid ◽  
Hplc Method ◽  
The One ◽  
Rutin And Quercetin ◽  
Red Currant

The extracts obtained from two types of berries: red currant and raspberries, were evaluated for their phenolic content. They were identified and quantified by using an optimized HPLC method. During the analyze several phenolic compounds were found, like: gallic acid, (+)- catechin, syringic acid, cinnamic acid, chlorogenic acid, ferulic acid, rutin and quercetin. The total amount of phenolic compounds analyzed found in red currant was greater than the one found in raspberries, due to the low variety of phenolic compounds extracted. The greatest amount of gallic acid, (+)- catechin, syringic acid, cinnamic acid, chlorogenic acid, ferulic acid and rutin was determined from the extraction of red currant berries and the raspberries extract contained the greatest source of quercetin. This study shows that red currant can provide the highest and most varied content of phenolic compounds from the analyzed berries.

Sign in / Sign up

Export Citation Format

Share Document