scholarly journals Novel Spectrophotometric Methods for the Determination of Selegiline Hydrochloride in Bulk and Its Pharmaceutical Preparation

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Kumble Divya ◽  
Badiadka Narayana
Keyword(s):  
Spectrophotometric Method ◽  
Pharmaceutical Preparation ◽  
Coupling Reaction ◽  
Pharmaceutical Formulations ◽  
Molar Absorptivity ◽  
Spectrophotometric Methods ◽  
Colored Product ◽  
Optimum Reaction ◽  
Optimum Reaction Condition

A simple and highly selective spectrophotometric method has been developed for the determination of selegiline hydrochloride in bulk and formulations. Method A is based on the oxidation of 3-methyl-2-benzothiazolinone hydrazone in the presence of ceric ammonium sulphate, followed by its coupling reaction with drug to form a colored product having λmax of 629 nm. Method B is based on the coupling reaction of drug with 4-aminoantipyrine to give a new ligand that reacts with copper(II) to give intense bluish red colored chelate which is measured at 539 nm. Beer’s law is obeyed in the range of 10.00–85.00 μg mL−1 with molar absorptivity of 0.98×104 for method A and 20.00–120.00 μg mL−1 with molar absorptivity of 0.94×104 for method B. The optimum reaction condition and the analytical parameters are evaluated. The results obtained indicate that the methods are free from interference of the ingredients; thus they are successfully applied to pharmaceutical formulations.

scholarly journals Spectrophotometric method for the determination of Captopril in pharmaceutical formulations

2013 ◽  
Vol 10 (3) ◽  
pp. 965-970
Author(s):  
Baghdad Science Journal
Keyword(s):  
Aqueous Solution ◽  
Spectrophotometric Method ◽  
Pharmaceutical Formulations ◽  
Molar Absorptivity ◽  
Maximum Absorption ◽  
Neutral Medium ◽  
Pharmaceutical Tablets ◽  
Colored Product ◽  
Sensitive Spectrophotometric Method

A simple, rapid and sensitive spectrophotometric method has been developed for the determination of captopril in aqueous solution. The method is based on reaction of captopril with 2,3-dichloro 1,4- naphthoquinon(Dichlone) in neutral medium to form a stable yellow colored product which shows maximum absorption at 347 nm with molar absorptivity of 5.6 ×103 L.mole-1. cm-1. The proposed method is applied successfully for determination of captopril in commercial pharmaceutical tablets.

scholarly journals Spectrophotometric Methods for the Assay of Pyrilamine Maleate Using Chromogenic Reagents

2010 ◽  
Vol 7 (4) ◽  
pp. 1507-1513 ◽  
Author(s):  
V. Annapurna ◽  
G. Jyothi ◽  
V. Nagalakshmi ◽  
B. B. V. Sailaja
Keyword(s):  
Charge Transfer ◽  
Oxidative Coupling ◽  
Coupling Reaction ◽  
Pharmaceutical Formulations ◽  
Molar Absorptivity ◽  
Method Of Least Squares ◽  
Spectrophotometric Methods ◽  
Oxidative Coupling Reaction ◽  
Charge Transfer Complexation

Simple, accurate and reproducible UV spectrophotometric methods were established for the assay of pyrilamine maleate (PYRA) based on the formation of oxidative coupling and precipitation, charge transfer complexation products. Method A includes the oxidative coupling reaction of PYRA with 3-methyl-2-benzathiazolinone hydrazone (MBTH) in presence of Ce(IV). The formation of oxidative coupling product with 4-amino phenazone (4-AP) in presence of K3Fe(CN)6is incorporated in method B. Precipitation/charge transfer complex formation of the PYRA with tannic acid (TA)/Metol-Cr(VI) in method C were proposed. The optical characteristics such as Beers law limits, molar absorptivity and Sandell’s sensitivity for the methods (A-C) are given. Regression analysis using the method of least squares was made to evaluate the slope (b), intercept (a) and correlation coefficient (r) and standard error of estimation (Se) for each system. Determination of pyrilamine in bulk form and in pharmaceutical formulations were also incorporated.

scholarly journals DEVELOPMENT AND VALIDATION OF SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF DPP-4 INHIBITOR, SITAGLIPTIN, IN ITS PHARMACEUTICAL PREPARATIONS

2018 ◽  
Vol 35 (3) ◽  
pp. 45
Author(s):  
C. Bala Sekaran ◽  
A. Prameela Rani
Keyword(s):  
Spectrophotometric Method ◽  
Pharmaceutical Preparations ◽  
Pharmaceutical Formulations ◽  
Molar Absorptivity ◽  
Amino Group ◽  
Reaction Conditions ◽  
Colored Product ◽  
Primary Amino ◽  
Development And Validation

A simple, sensitive and reproducible spectrophotometric method was developed for the determination of sitagliptin phosphate in bulk and in pharmaceutical formulations. The proposed method is based on condensation of the primary amino group of sitagliptin phosphate with acetyl acetone and formaldehyde producing a yellow colored product, which is measured spectrophotometrically at 430nm. The color was stable for about 1 hour. Beer’s law is obeyed over a concentration range of 5-25 μg/ml. The apparent molar absorptivity and Sandell sensitivity values are 1.067 x 104 Lmol-1cm-1 and 0.0471 μgcm-2 respectively. All the variables were studied to optimize the reaction conditions. No interference was observed in the presence of common pharmaceutical excipients. The validity of the method was tested by analyzing sitagliptin phosphate in its pharmaceutical preparations. Good recoveries were obtained. The developed method was successfully employed for the determination of sitagliptin phosphate in various pharmaceutical preparations.

scholarly journals SPECTROPHOTOMETRIC DETERMINATION OF NEVIRAPINE USING TETRATHIOCYANATOCOBALT(II) ION AS A REAGENT

2018 ◽  
Vol 35 (3) ◽  
pp. 93
Author(s):  
T. V. Badiadka ◽  
Badiadka Narayana
Keyword(s):  
Detection Limit ◽  
Spectrophotometric Determination ◽  
Spectrophotometric Method ◽  
Statistical Evaluation ◽  
Pharmaceutical Formulations ◽  
Molar Absorptivity ◽  
Optical Parameters ◽  
Quantitation Limit ◽  
Optimum Reaction

A simple and rapid spectrophotometric method for the determination of nevirapine is described. The method is based on the reaction of nevirapine with tetrathiocyanatocobalt(II) ion in buffer of pH 4 to form the corresponding complex. Beer’s law is obeyed in the range of 0.2 – 2.0 μg mL-1 for nevirapine. The optical parameters such as molar absorptivity, Sandell’s sensitivity, detection limit and quantitation limit were found to be 1.16× 104 Lmol-1cm-1, 2.09 X 10-3 μg cm-2, 0.073 μg mL-1 and 0.222 μg mL-1 respectively. The optimum reaction conditionsand other analytical parameters were evaluated. The statistical evaluation of the method was examined by determining intra-day and inter-day precision. The proposed method has been successfully applied for the determination of nevirapine in pharmaceutical formulations.

scholarly journals Spectrophotometric method for the determination of benzocaine by cerium ammonium sulphate with promethazine hydrochloride in pure and phar-maceuticals preparation

2019 ◽  
Vol 10 (2) ◽  
pp. 1420-1423 ◽  
Author(s):  
Amal H. Mhemeed
Keyword(s):  
Ammonium Sulphate ◽  
Spectrophotometric Method ◽  
Oxidative Coupling ◽  
Pharmaceutical Preparation ◽  
Coupling Reaction ◽  
Molar Absorptivity ◽  
Oxidative Coupling Reaction ◽  
Promethazine Hydrochloride ◽  
Cerium Ammonium

Easy, the accurate, sensitive spectrophotometric method used to determine Benzocaine in a pure and pharmaceutical preparation. The proposed method depends on using Promethazine hydrochloride as a reagent. Where it depends on oxidative coupling reaction of benzocaine by cerium ammonium sulphate with promethazine hydrochloride in the presence of hydrochloric acid to form green dye product, lmax 615 nm. Beers law is obeyed in the concentration of (5 –300 µg.ml-1). The molar absorptivity is (1.77 * 10 3) L.mol -1.cm-1, a sandal sensitivity of (0.0098) µq.cm-2 and RSD (± 0.56 %). The method gave a successful determination for benzocaine in a pharmaceutical preparation.

scholarly journals Estimation of Furosemide in Pharmaceutical Preparation Samples

2020 ◽  
Vol 1 (1) ◽  
Keyword(s):  
Standard Deviation ◽  
Spectrophotometric Method ◽  
Pharmaceutical Preparation ◽  
Pharmaceutical Preparations ◽  
Pharmaceutical Formulations ◽  
Molar Absorptivity ◽  
Oral Solution ◽  
Average Recovery ◽  
Relative Standard

A simple, accurate, precise, rapid, economical and sensitive ultraviolet spectrophotometric method has been developed for the determination of Furosemide in pharmaceutical preparations, which shows maximum absorbance at 228 nm in. Beer’s law was obeyed in the range of 1 -10 μg/ ml, with molar absorptivity of 3.5×104 L.mol-1.cm-1, relative standard deviation of the method was less than 1.4%, and accuracy (average recovery %) was 100 ± 1.0. The method was successfully applied to the determination of Furosemide in some pharmaceutical formulations (tablets, Oral solution and injection) samples. The proposed method was validated by sensitivity and precision which proves suitability for the routine analysis of Furosemide in true samples.

Extractive Spectrophotometric Method for the Determination of Glibenclamide by Ion-Pair Complex in Pure Form and Pharmaceutical Formulation

2019 ◽  
Vol 9 (o3) ◽  
Author(s):  
RUAA MUAYAD MAHMOOD ◽  
HAMSA MUNAM YASSEN ◽  
SAMAR , NAJWA ISSAC ABDULLA AHMED DARWEESH ◽  
NAJWA ISSAC ABDULLA
Keyword(s):  
Spectrophotometric Method ◽  
Molar Absorptivity ◽  
Ion Pair ◽  
Pharmaceutical Formulation ◽  
Ratio Method ◽  
Colored Complex ◽  
Colored Product ◽  
Method Of Continuous Variation ◽  
Job's Method

Simple, rapid and sensitive extractive spectrophotometric method is presented for the determination of glibenclamide (Glb) based on the formation of ion-pair complex between the Glb and anionic dye, methyl orange (MO) at pH 4. The yellow colored complex formed was quantitatively extracted into dichloromethane and measured at 426 nm. The colored product obeyed Beer’s law in the concentration range of (0.5-40) μg.ml-1. The value of molar absorptivity obtained from Beer’s data was found to be 31122 L.mol-1.cm-1, Sandell’s sensitivity value was calculated to be 0.0159 μg.cm-2, while the limits of detection (LOD) and quantification (LOQ) were found to be 0.1086 and 0.3292 μg.ml-1, respectively. The stoichiometry of the complex created between the Glb and MO was 1:1 as determined via Job’s method of continuous variation and mole ratio method. The method was successfully applied for the analysis of pharmaceutical formulation.

scholarly journals New Diazo Coupling Reactions for Visible Spectrophotometric Determination of Alfuzosin in Pharmaceutical Preparations

2007 ◽  
Vol 4 (4) ◽  
pp. 496-501 ◽  
Author(s):  
M. Vamsi Krishna ◽  
D. Gowri Sankar
Keyword(s):  
Pharmaceutical Preparations ◽  
Pharmaceutical Formulations ◽  
Molar Absorptivity ◽  
Basic Medium ◽  
Coupling Reactions ◽  
Acetyl Acetone ◽  
Diazo Coupling ◽  
Optimum Reaction ◽  
Diazonium Ion

Simple, rapid and sensitive spectrophotometric procedures were developed for the analysis of Alfuzosin hydrochloride (AFZ) in pure form as well as in pharmaceutical formulations. The methods are based on the reaction of AFZ with nitrite in acid medium to form diazonium ion, which is coupled with ethoxyethylenemaleic ester (Method A) or ethylcyanoacetate (Method B) or acetyl acetone (method C) in basic medium to form azo dyes, showing absorption maxima at 440, 465 and 490 nm respectively. Beer’s law is obeyed in the concentration of 4-20 μg/mL of AFZ for methods A, B and 3-15 μg/mL of AFZ for method C. The molar absorptivity and sandell’s sensitivity of AFZ- ethoxyethylenemaleic ester, AFZ- ethylcyanoacetate and AFZ-acetyl acetone are1.90 × 104, 0.022; 1.93 × 104, 0.021 and 2.67 × 104L mole-1cm-1, 0.015 μg cm-2respectively. The optimum reaction conditions and other analytical parameters were evaluated. The methods were successfully applied to the determination of AFZ in pharmaceutical formulations.

scholarly journals Indirect spectrophotometric determination of gentamicin and vancomycin antibiotics based on their oxidation by potassium permanganate

2006 ◽  
Vol 4 (4) ◽  
pp. 708-722 ◽  
Author(s):  
Akram El-Didamony ◽  
Alaa Amin ◽  
Ahmed Ghoneim ◽  
Ayman Telebany
Keyword(s):  
Acid Medium ◽  
Potassium Permanganate ◽  
Pharmaceutical Formulations ◽  
Molar Absorptivity ◽  
Reaction Conditions ◽  
Subsequent Determination ◽  
Optimum Reaction ◽  
Acid Orange Ii ◽  
Sulphuric Acid Medium

AbstractFour simple, accurate, sensitive and economical procedures (A–D) for the estimation of gentamicin sulphate and vancomycin hydrochloride, both in pure form and in pharmaceutical formulations have been developed. The methods are based on the oxidation of the studied drugs by a known excess of potassium permanganate in sulphuric acid medium and subsequent determination of unreacted oxidant by reacting it with amaranth dye (method A), acid orange II (method B), indigocarmine (method C) and methylene blue (method D), in the same acid medium at a suitable λmax=521, 485, 610 and 664 nm, respectively. The reacted oxidant corresponds to the drug content. Regression analysis of Beer-Lambert plots showed good correlations in the concentration ranges 4–8, 3–8, 4–9 and 5–9 µg ml−1 with gentamicin and 4–8, 1.5–4, 1.5–4 and 3.5–5.5 µg ml−1 with vancomycin for methods A, B, C, and D, respectively. The molar absorptivity, sandell sensitivity, detection and quantification limits were calculated. The stoichiometric ratios for the cited drugs were studied. The optimum reaction conditions and other analytical parameters were evaluated. The influence of the substance commonly employed as excipients with these drugs were studied. The proposed methods were applied to the determination of these drugs in pharmaceutical formulations. The results have demonstrated that the methods are equally accurate and reproducible as the official methods.

scholarly journals Simple Spectrophotometric Method for Determination of Paroxetine in Tablets Using 1,2-Naphthoquinone-4-Sulphonate as a Chromogenic Reagent

2009 ◽  
Vol 2009 ◽  
pp. 1-8 ◽  
Author(s):  
Ibrahim A. Darwish ◽  
Heba H. Abdine ◽  
Sawsan M. Amer ◽  
Lama I. Al-Rayes
Keyword(s):  
Spectrophotometric Method ◽  
Correlation Coefficients ◽  
Molar Absorptivity ◽  
Official Method ◽  
Calibration Data ◽  
Pharmaceutical Tablets ◽  
Relative Standard ◽  
Label Claim ◽  
Colored Product

Simple and rapid spectrophotometric method has been developed and validated for the determination of paroxetine (PRX) in tablets. The proposed method was based on nucleophilic substitution reaction of PRX with 1,2-naphthoquinone-4-sulphonate (NQS) in an alkaline medium to form an orange-colored product of maximum absorption peak () at 488 nm. The stoichiometry and kinetics of the reaction were studied, and the reaction mechanism was postulated. Under the optimized reaction conditions, Beer's law correlating the absorbance (A) with PRX concentration (C) was obeyed in the range of 1–8 g . The regression equation for the calibration data was: A = 0.0031 + 0.1609 C, with good correlation coefficients (0.9992). The molar absorptivity () was L  1 . The limits of detection and quantitation were 0.3 and 0.8 g , respectively. The precision of the method was satisfactory; the values of relative standard deviations did not exceed 2%. The proposed method was successfully applied to the determination of PRX in its pharmaceutical tablets with good accuracy and precisions; the label claim percentage was %. The results obtained by the proposed method were comparable with those obtained by the official method.

Sign in / Sign up

Export Citation Format

Share Document