scholarly journals Preparation, Characterization, and Bioactivity of Chitosan Microspheres Containing Basic Fibroblast Growth Factor

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Bo Lv ◽  
Yue Wang ◽  
Wei Chen
Keyword(s):  
Cell Viability ◽  
Three Dimensional ◽  
Spherical Shape ◽  
S Phase ◽  
Drying Process ◽  
Surface Charges ◽  
Chitosan Microspheres ◽  
Porous Chitosan ◽  
Cell Growth And Proliferation

The aim of this study is to evaluate, prepare, and characterize bioactivity of chitosan microspheres loaded with bFGF for providing sustained release of bFGF. Porous chitosan microspheres were prepared by freeze-drying process based on the interaction between chitosan and tripolyphosphate (TPP). The bFGF-loaded chitosan microspheres were well interconnected and have a narrow size distribution, spherical shape, and positive surface charges. The bFGF-loading capacity and encapsulation efficiency were 7.57 mg/g and 95.1%, respectively. Results ofin vitrorelease showed that the extent of release was 82.1% at Day 25. Schwann cells were used as anin vitromodel for cell response to bFGF and bFGF-loaded chitosan microspheres. Results indicated that the number, cell viability, and percentage of cells G2/M+S phase in the bFGF groups are higher than those in the bFGF-loaded chitosan microspheres groups before culturing for 2 days. However, the number, cell viability, and percent of cells G2/M+S phase in the bFGF-loaded chitosan microspheres groups are significantly higher than those in the bFGF groups after culture for 4 and 8 days. These findings indicated that bFGF-loaded chitosan microspheres may help to decrease the release of bFGF and provide a suitable three-dimensional environment for cell growth and proliferation.

scholarly journals The Potential of a Tailored Biomimetic Hydrogel for In Vitro Cell Culture Applications: Characterization and Biocompatibility

2020 ◽  
Vol 10 (24) ◽  
pp. 9035
Author(s):  
Yung-Chieh Cho ◽  
Hsiao-Ting Huang ◽  
Wen-Chien Lan ◽  
Mao-Suan Huang ◽  
Takashi Saito ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Viability ◽  
Three Dimensional ◽  
Spherical Shape ◽  
Hair Follicles ◽  
Pluronic F127 ◽  
Cell Counting ◽  
Differentiation Potential ◽  
Swiss 3T3

In this study, the Pluronic F127 with modified tripeptide Gly-Arg-Gly-Asp copolymer (hereafter defined as 3BE) hydrogel was evaluated in terms of its biocompatibility potentials. The fibroblasts (Swiss 3T3 cell line) and human hair follicles-derived mesenchymal stem cells (HFMSCs) were cultured in different concentrations of the 3BE hydrogel (0%, 0.05%, 0.1%, 0.25%, and 0.5%, respectively). The cell morphology and differentiation potential of HFMSCs were observed through optical microscopy, and the cell viability was investigated via Live/Dead Kit and Cell Counting Kit-8 assay. Analytical results showed that HFMSC can differentiate into adipogenic, chondrogenic, and osteogenic lineages. The HFMSC and Swiss 3T3 cells would properly assemble into a spherical shape as cultured with the 3BE hydrogel. Most importantly, cell viability could be maintained above 70%. The formation of spheroid structures of cells within this hydrogel is predicted to promote cell differentiation potentials of HFMSC that benefit in generating functional adipocytes, chondrocytes, and osteoblasts. Therefore, these findings demonstrate that the 3BE hydrogel has great potential as a three-dimensional cell culture scaffold for tissue engineering applications.

In vitro and in vivo evaluation of porous chitosan electret membrane for bone regeneration

2018 ◽  
Vol 33 (4) ◽  
pp. 426-438 ◽  
Author(s):  
Yanying Wang ◽  
Xiaodi Sun ◽  
Qingfu Wang ◽  
Jing Yang ◽  
Ping Gong ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Three Dimensional ◽  
Surface Charges ◽  
In Vivo Evaluation ◽  
Thermally Induced ◽  
Cell Proliferation And Differentiation ◽  
Porous Chitosan ◽  
Calvarial Defects

A porous chitosan electret membrane, possessing a three-dimensional porous structure and surface charges, was developed using thermally induced phase separation method and grid-controlled corona charging. Results showed that surface charge release of porous electret membrane could be altered by varying charging voltage. Rat osteoblasts adhered well, and cell proliferation and differentiation were enhanced by porous electret membrane compared to porous uncharged membrane. Furthermore, rabbit calvarial defects model demonstrated that porous electret membrane promoted bone regeneration more significantly than porous uncharged membrane. Therefore, the porous chitosan electret membrane might be a promising material for bone regeneration and bone tissue engineering applications.

Porous chitosan microspheres for application as quick in vitro and in vivo hemostat

2017 ◽  
Vol 77 ◽  
pp. 411-419 ◽  
Author(s):  
Jixiang Li ◽  
Xiaowei Wu ◽  
Yanqing Wu ◽  
Zonghao Tang ◽  
Xun Sun ◽  
...  
Keyword(s):  
Chitosan Microspheres ◽  
Porous Chitosan

scholarly journals Multiresponsive Hybrid Microparticles for Stimuli-Responsive Delivery of Bioactive Compounds

2020 ◽  
Vol 10 (12) ◽  
pp. 4324 ◽  
Author(s):  
Sergei S. Vlasov ◽  
Pavel S. Postnikov ◽  
Mikhail V. Belousov ◽  
Sergei V. Krivoshchekov ◽  
Mekhman S. Yusubov ◽  
...  
Keyword(s):  
Cell Viability ◽  
Spherical Shape ◽  
In Vitro Cytotoxicity ◽  
Poly Lactic Acid ◽  
Iron Core ◽  
Stimuli Responsive ◽  
Ultrasonic Fields ◽  
Burst Intensity ◽  
Free Doxorubicin

Hybrid microparticles based on an iron core and an amphiphilic polymeric shell have been prepared to respond simultaneously to magnetic and ultrasonic fields and variation in the surrounding pH to trigger and modulate the delivery of doxorubicin. The microparticles have been developed in four steps: (i) synthesis of the iron core; (ii) surface modification of the core; (iii) conjugation with the amphiphilic poly(lactic acid)-grafted chitosan; and (iv) doxorubicin loading. The particles demonstrate spherical shape, a size in the range of 1–3 µm and surface charge that is tuneable by changing the pH of the environment. The microparticles demonstrate good stability in simulated physiological solutions and are able to hold up to 400 µg of doxorubicin per mg of dried particles. The response to ultrasound and the changes in the shell structure during exposure to different pH levels allows the control of the burst intensity and release rate of the payload. Additionally, the magnetic response of the iron core is preserved despite the polymer coat. In vitro cytotoxicity tests performed on fibroblast NIH/3T3 demonstrate a reduction in the cell viability after administration of doxorubicin-loaded microparticles compared to the administration of free doxorubicin. The application of ultrasound causes a burst in the release of the doxorubicin from the carrier, causing a decrease in cell viability. The microparticles demonstrate in vitro cytocompatibility and hemocompatibility at concentrations of up to 50 and 60 µg/mL, respectively.

Tissue Engineered Human Septal Cartilage in a Murine Model

2008 ◽  
Vol 139 (2_suppl) ◽  
pp. P87-P88
Author(s):  
Angela Chang ◽  
Sage August ◽  
Barbara L Schumacher ◽  
Williams Gregory ◽  
Robert L Sah ◽  
...  
Keyword(s):  
Cell Viability ◽  
Murine Model ◽  
Three Dimensional ◽  
Alginate Beads ◽  
Septal Cartilage ◽  
Hematoxylin And Eosin ◽  
Matrix Production ◽  
Nasal Septal Cartilage

Problem Tissue engineering of human nasal septal cartilage represents an alternative technique for creating large quantities of autologous material for use in reconstructive surgery of the head and neck. Septal neocartilage constructs developed in vitro by the alginate method have demonstrated cartilaginous extracellular matrix production, but their biocompatibility and development in vivo remains largely unknown. Methods A murine model was used to examine the behavior of neocartilage constructs in vivo. Chondrocytes collected from donors undergoing septoplasty were expanded in monolayer and suspended in alginate beads for three-dimensional culture in media containing human serum and growth factors. After in vitro incubation for 5 weeks, the neocartilage constructs were implanted subcutaneously in the dorsum of athymic mice for 30 days (n=3). The mice were sacrificed and the constructs were explanted for assessment of cell viability, gross morphology, and histology. Results The mice survived and tolerated the implant well. Infection and extrusion were not observed. Neocartilage constructs maintained their general shape and size, and demonstrated cell viability after implantation. Explanted constructs were firm and opaque, sharing closer semblance to native septal tissue relative to the gelatinous, translucent pre-implant constructs. On hematoxylin and eosin staining, the explanted constructs exhibited distinct morphologies characteristic of native tissue, which were not observed in pre-implant constructs. Conclusion Neocartilage constructs are viable in an in vivo murine model. The morphologic and histologic features of explanted constructs more closely resemble native septal tissue when compared to pre-implant constructs. Significance Septal neocartilage constructs are biocompatible and demonstrate potential for in vivo maturation with eventual clinical application.

scholarly journals Rational Design of a Triple-Layered Coaxial Extruder System: in silico and in vitro Evaluations Directed Towards Optimizing Cell Viability

2020 ◽  
Vol 6 (4) ◽  
Author(s):  
Christian Augusto Silva ◽  
Carlos J Cortés-Rodriguez ◽  
Jonas Hazur ◽  
Supachai Reakasame ◽  
Aldo R. Boccaccini
Keyword(s):  
Cell Viability ◽  
In Silico ◽  
Rational Design ◽  
Three Dimensional ◽  
Complex Structures ◽  
Vascular Networks ◽  
Coaxial Nozzle ◽  
Wide Availability ◽  
Cell Functionality

Biofabrication is a rapidly evolving field whose main goal is the manufacturing of three-dimensional (3D) cell-laden constructs that closely mimic tissues and organs. Despite recent advances on materials and techniques directed toward the achievement of this goal, several aspects such as tissue vascularization and prolonged cell functionality are limiting bench-to-bedside translation. Extrusion-based 3D bioprinting has been devised as a promising biofabrication technology to overcome these limitations, due to its versatility and wide availability. Here, we report the development of a triple-layered coaxial nozzle for use in the biomanufacturing of vascular networks and vessels. The design of the coaxial nozzle was first optimized toward guaranteeing high cell viability upon extrusion. This was done with the aid of in silico evaluations and their subsequent experimental validation by investigating the bioprinting of an alginate-based bioink. Results confirmed that the values for pressure distribution predicted by in silico experiments resulted in cell viabilities above 70% and further demonstrated the effect of layer thickness and extrusion pressure on cell viability. Our work paves the way for the rational design of multi-layered coaxial extrusion systems to be used in biofabrication approaches to replicate the very complex structures found in native organs and tissues.

scholarly journals Three-Dimensional Reconstructed Bone Marrow Matrix Culture Improves the Viability of Primary Myeloma Cells In-Vitro via a STAT3-Dependent Mechanism

2021 ◽  
Vol 43 (1) ◽  
pp. 313-323
Author(s):  
Yung-Hsing Huang ◽  
Meaad Almowaled ◽  
Jing Li ◽  
Christopher Venner ◽  
Irwindeep Sandhu ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Cell Viability ◽  
Mononuclear Cells ◽  
Three Dimensional ◽  
3D Culture ◽  
Trypan Blue Exclusion ◽  
Conventional Culture ◽  
Significant Difference ◽  
Dependent Mechanism

Primary myeloma (PM) cells are short-lived in conventional culture, which limited their usefulness as a study model. Here, we evaluated if three-dimensional (3D) culture can significantly prolong the longevity of PM cells in-vitro. We employed a previously established 3D model for culture of bone marrow mononuclear cells isolated from 15 patients. We assessed the proportion of PM cells, viability and proliferation using CD38 staining, trypan blue exclusion assays and carboxy fluorescein succinimidyl ester (CFSE) staining, respectively. We observed significantly more CD38+ viable cells in 3D than in conventional culture (65% vs. 25%, p = 0.006) on day 3. CFSE staining showed no significant difference in cell proliferation between the two culture systems. Moreover, we found that PM cells in 3D culture are more STAT3 active by measure of pSTAT3 staining (66% vs. 10%, p = 0.008). Treatment of IL6, a STAT3 activator significantly increased CD38+ cell viability (41% to 68%, p = 0.021). In comparison, inhibition of STAT3 with Stattic significantly decreased PM cell viability in 3D culture (38% to 17% p = 0.010). Neither IL6 nor Stattic affected the PM cell viability in conventional culture. This study suggests that 3D culture can significantly improve the longevity of PM cells in-vitro, and STAT3 activation can further improve their viability.

scholarly journals HMT Exerts an Anticancer Effect by Targeting PAK-1

2021 ◽  
Vol 11 (13) ◽  
pp. 6034
Author(s):  
Yinzhu Xu ◽  
Jin-Sol Cha ◽  
Seon-Ok Lee ◽  
Soo-In Ryu ◽  
You-Kyung Lee ◽  
...  
Keyword(s):  
Cell Viability ◽  
Three Dimensional ◽  
3D Cell Culture ◽  
Inhibitory Effect ◽  
Functional Health ◽  
G1 Arrest ◽  
Health Food ◽  
Cell Viability Assay ◽  
Migration Assay

Heamatang (HMT) is a classic medicinal formula used in traditional Chinese and Korean medicine; it contains seven distinct components, mainly of herbal origin. HMT is used as an antiaging remedy, treating urinary disorders and increasing energy and vitality. However, the therapeutic applications of this formula have not been evaluated with evidence-based science. Therefore, we assessed HMT through various in vitro methods, including cell viability assay, fluorescence-activated cell sorting assay (FACS), Western blotting, migration assay, three-dimensional (3D) cell culture, siRNA-mediated PAK-1 knockdown, and crystal violet assays. HMT decreased PAK-1 expression in PC-3 cells and inhibited cell viability, growth, and motility. The inhibition of cell motility by HMT was correlated with PAK-1-mediated inhibition of Lim domain kinase (LIMK) and cofilin. HMT induced G1 arrest and apoptosis through the transcriptional regulation of cell cycle regulatory proteins and apoptosis-related proteins (increase in c-cas3 and inhibition of PARP and BCL-2). Moreover, HMT suppressed PAK-1 expression, leading to the inhibition of AKT activities. Finally, we showed that decursin was the active ingredient involved in the inhibitory effect of HMT on PAK-1. Our findings demonstrated that HMT exerts its anticancer influence through the inhibition of PAK-1. The HMT formula could be applied in various fields, including functional health food and pharmaceutical development.

scholarly journals Cellular Nutrition in Complex Three-Dimensional Scaffolds: A Comparison between Experiments and Computer Simulations

2015 ◽  
Vol 2015 ◽  
pp. 1-12 ◽  
Author(s):  
Claudia Bergemann ◽  
Patrick Elter ◽  
Regina Lange ◽  
Volker Weißmann ◽  
Harald Hansmann ◽  
...  
Keyword(s):  
Shear Stress ◽  
Cell Viability ◽  
Computer Simulations ◽  
Flow Rate ◽  
Oxygen Supply ◽  
Three Dimensional ◽  
Flow Rates ◽  
The Core ◽  
Local Oxygen

Studies on bone cell ingrowth into synthetic, porous three-dimensional (3D) implants showed difficulties arising from impaired cellular proliferation and differentiation in the core region of these scaffolds with increasing scaffold volumein vitro. Therefore, we developed anin vitroperfusion cell culture module, which allows the analysis of cells in the interior of scaffolds under different medium flow rates. For each flow rate the cell viability was measured and compared with results from computer simulations that predict the local oxygen supply and shear stress inside the scaffold based on the finite element method. We found that the local cell viability correlates with the local oxygen concentration and the local shear stress. On the one hand the oxygen supply of the cells in the core becomes optimal with a higher perfusion flow. On the other hand shear stress caused by high flow rates impedes cell vitality, especially at the surface of the scaffold. Our results demonstrate that both parameters must be considered to derive an optimal nutrient flow rate.

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