scholarly journals Echocardiographic Assessment of Embryonic and Fetal Mouse Heart Development: A Focus on Haemodynamics and Morphology

2014 ◽  
Vol 2014 ◽  
pp. 1-11 ◽  
Author(s):  
Nathan D. Hahurij ◽  
Emmeline E. Calkoen ◽  
Monique R. M. Jongbloed ◽  
Arno A. W. Roest ◽  
Adriana C. Gittenberger-de Groot ◽  
...  
Keyword(s):  
Heart Development ◽  
High Frequency ◽  
Developmental Stages ◽  
Imaging Modality ◽  
Abnormal Development ◽  
Mouse Heart ◽  
High Frequency Ultrasound ◽  
Flow Measurements ◽  
Fetal Mouse ◽  
Systolic And Diastolic Function

Background. Heart development is a complex process, and abnormal development may result in congenital heart disease (CHD). Currently, studies on animal models mainly focus on cardiac morphology and the availability of hemodynamic data, especially of the right heart half, is limited. Here we aimed to assess the morphological and hemodynamic parameters of normal developing mouse embryos/fetuses by using a high-frequency ultrasound system.Methods. A timed breeding program was initiated with a WT mouse line (Swiss/129Sv background). All recordings were performed transabdominally, in isoflurane sedated pregnant mice, in hearts of sequential developmental stages: 12.5, 14.5, and 17.5 days after conception (n=105).Results. Along development the heart rate increased significantly from 125 ± 9.5 to 219 ± 8.3 beats per minute. Reliable flow measurements could be performed across the developing mitral and tricuspid valves and outflow tract. M-mode measurements could be obtained of all cardiac compartments. An overall increase of cardiac systolic and diastolic function with embryonic/fetal development was observed.Conclusion. High-frequency echocardiography is a promising and useful imaging modality for structural and hemodynamic analysis of embryonic/fetal mouse hearts.

Diagnostic Value of Dynamic High-Frequency Ultrasound for the Slipping Rib and twelfth Rib Syndrome: A Case Series With Review.

2020 ◽  
Vol 16 ◽  
Author(s):  
Nalin G. Patel ◽  
Dhruvkumar M. Patel ◽  
Mukundkumar V. Patel ◽  
Maitri M. Patel ◽  
Tanmay R. Patel ◽  
...  
Keyword(s):  
Nerve Block ◽  
High Frequency ◽  
Imaging Modality ◽  
Case Series ◽  
Diagnostic Value ◽  
High Frequency Ultrasound ◽  
Imaging Tool ◽  
Loin Pain ◽  
Upper Abdominal ◽  
Frequency Ultrasound

Background: High-frequency ultrasound (HFUS) is a mobile, radiation-free imaging tool for the diagnosis of musculoskeletal disorders. We aim to demonstrate the diagnostic value of dynamic HFUS for undiagnosed lower chest, upper abdomen, and loin pain with this case series. Case series: A cricketer presented with long-standing left-sided dull ache lower chest and upper abdominal pain, aggravated on exertion and leaning forward. His previous laboratory and previous imaging tests were unrevealing. Dynamic HFUS of his left ribs during hooking maneuver demonstrated slipping of the eighth rib over the seventh rib associated with clicking. He also reported tenderness over this region. He was diagnosed as slipping rib syndrome (SRS), and was treated with the eighth nerve block under the HFUS guidance. The second and third cases presented with chronic undiagnosed waxing and waning loin pain despite extensive laboratory and radiological workup. Both patients demonstrated twelfth rib HFUS probe tenderness in sitting position with a specific movement that reproduced the pain during dynamic HFUS study. The diagnosis of twelfth rib syndrome (TRS) was confirmed, and treated successfully with a local intercostal nerve block. Review of the literature: HFUS is the most underutilized imaging tool for the diagnosis of unexplained upper abdominal and lower chest pain syndromes. We identified only a few such reported cases managed with the help of HFUS. Conclusion: The dynamic HFUS is a valuable imaging modality for the undiagnosed lower chest, upper abdominal, or loin pain.

A Complete Computational Model of Ultrasound Vibro-Acoustography

2006 ◽  
Author(s):  
Alison E. Malcolm ◽  
Fernando Reitich ◽  
Jiaqi Yang ◽  
Mostafa Fatemi ◽  
James F. Greenleaf
Keyword(s):  
High Resolution ◽  
Optimal Design ◽  
Computational Model ◽  
High Frequency ◽  
Imaging Modality ◽  
Low Frequency ◽  
High Frequency Ultrasound ◽  
Linear Interaction ◽  
Modeling Simulation ◽  
Frequency Ultrasound

Ultrasound vibro-acoustography is a novel medical imaging modality that combines the high resolution of high-frequency ultrasound with the speckle-free images obtained using low-frequency methods. This imaging modality relies on the non-linear interaction of two high frequency beams at slightly different frequencies. We describe the physics of ultrasound vibro-acoustography and outline a strategy for its modeling, simulation, and optimal design.

scholarly journals Dynamic Patterns of N6-Methyladenosine Profiles of Messenger RNA Correlated with the Cardiomyocyte Regenerability during the Early Heart Development in Mice

2021 ◽  
Vol 2021 ◽  
pp. 1-15
Author(s):  
Yuhui Yang ◽  
Siman Shen ◽  
Yin Cai ◽  
Kejun Zeng ◽  
Keyu Liu ◽  
...  
Keyword(s):  
Heart Development ◽  
Messenger Rna ◽  
Developmental Stages ◽  
Stop Codon ◽  
Wnt Signaling Pathway ◽  
Receptor Interaction ◽  
Mouse Heart ◽  
Newborn Mice ◽  
Myocardial Development ◽  
Old Mice

N6-Methyladenosine (m6A) plays important roles in regulating mRNA processing. Despite rapid progress in this field, little is known about the role and mechanism of m6A modification in myocardial development and cardiomyocyte regeneration. Existing studies have shown that the heart tissues of newborn mice have the capability of proliferation and regeneration, but its mechanism, particularly its relation to m6A methylation, remains unknown. Methods. To systematically profile the mRNA m6A modification pattern in the heart tissues of mice at different developmental stages, we jointly performed methylated RNA immunoprecipitation sequencing (MeRIP-seq) and RNA sequencing (RNA-seq) of heart tissues of mice, respectively, aged 1 day old, 7 days old, and 28 days old. Results. We identified the linkages and association between differentially expressed mRNA transcripts and hyper or hypomethylated m6A peaks in C57BL/6J mice at different heart developmental stages. Results showed that the amount of m6A peaks and the level of m6A modification were the lowest in the heart of mice at 1 day old. By contrast, heart tissues from 7-day-old mice tended to possess the most m6A peaks and the highest global m6A level. However, the m6A characteristics of myocardial tissue changed little after 7 days old as compared to that of 1 day old. Specifically, we found 1269 downmethylated genes of 1434 methylated genes in 7-day-old mouse heart tissues as compared to those in 1-day-old mice. Hypermethylation of some specific genes may correlate with the heart’s strong proliferative and regenerative capability at the first day after birth. In terms of m6A density, the tendency shifted from coding sequences (CDS) to 3 ′ -untranslated regions (3 ′ UTR) and stop codon with the progression of heart development. In addition, some genes demonstrated remarkable changes both in methylation and expression, like kiss1, plekha6, and megf6, which may play important roles in proliferation. Furthermore, signaling pathways highly related to proliferation such as “Wnt signaling pathway,” “ECM-receptor interaction,” and “cardiac chamber formation” were significantly enriched in 1-day-old methylated genes. Conclusions. Our results reveal a pattern that different m6A modifications are distributed in C57BL/6J heart tissue at different developmental stages, which provides new insights into a novel function of m6A methylation of mRNA in myocardial development and regeneration.

scholarly journals High-frequency ultrasound in clinical dermatology: a review

2021 ◽  
Vol 13 (1) ◽  
Author(s):  
Jack Levy ◽  
Devon L. Barrett ◽  
Nile Harris ◽  
Jiwoong Jason Jeong ◽  
Xiaofeng Yang ◽  
...  
Keyword(s):  
High Frequency ◽  
Imaging Modality ◽  
Normal Skin ◽  
Objective Assessment ◽  
Deep Fascia ◽  
High Frequency Ultrasound ◽  
Diagnostic Management ◽  
Cosmetic Dermatology ◽  
Frequency Ultrasound ◽  
Clinical Dermatology

Abstract Background Ultrasound was first introduced in clinical dermatology in 1979. Since that time, ultrasound technology has continued to develop along with its popularity and utility. Main text summary Today, high-frequency ultrasound (HFUS), or ultrasound using a frequency of at least 10 megahertz (MHz), allows for high-resolution imaging of the skin from the stratum corneum to the deep fascia. This non-invasive and easy-to-interpret tool allows physicians to assess skin findings in real-time, enabling enhanced diagnostic, management, and surgical capabilities. In this review, we discuss how HFUS fits into the landscape of skin imaging. We provide a brief history of its introduction to dermatology, explain key principles of ultrasonography, and review its use in characterizing normal skin, common neoplasms of the skin, dermatologic diseases and cosmetic dermatology. Conclusion As frequency advancements in ultrasonography continue, the broad applications of this imaging modality will continue to grow. HFUS is a fast, safe and readily available tool that can aid in diagnosing, monitoring and treating dermatologic conditions by providing more objective assessment measures.

scholarly journals A reference map of murine cardiac transcription factor chromatin occupancy identifies dynamic and conserved enhancers

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Brynn N. Akerberg ◽  
Fei Gu ◽  
Nathan J. VanDusen ◽  
Xiaoran Zhang ◽  
Rui Dong ◽  
...  
Keyword(s):  
Heart Development ◽  
Molecular Mechanisms ◽  
Developmental Stages ◽  
Transcriptional Regulatory Network ◽  
Regulatory Elements ◽  
Chromatin Accessibility ◽  
Specific Gene ◽  
Mouse Heart ◽  
Transcriptional Enhancer ◽  
Enhancer Activity

Abstract Mapping the chromatin occupancy of transcription factors (TFs) is a key step in deciphering developmental transcriptional programs. Here we use biotinylated knockin alleles of seven key cardiac TFs (GATA4, NKX2-5, MEF2A, MEF2C, SRF, TBX5, TEAD1) to sensitively and reproducibly map their genome-wide occupancy in the fetal and adult mouse heart. These maps show that TF occupancy is dynamic between developmental stages and that multiple TFs often collaboratively occupy the same chromatin region through indirect cooperativity. Multi-TF regions exhibit features of functional regulatory elements, including evolutionary conservation, chromatin accessibility, and activity in transcriptional enhancer assays. H3K27ac, a feature of many enhancers, incompletely overlaps multi-TF regions, and multi-TF regions lacking H3K27ac retain conservation and enhancer activity. TEAD1 is a core component of the cardiac transcriptional network, co-occupying cardiac regulatory regions and controlling cardiomyocyte-specific gene functions. Our study provides a resource for deciphering the cardiac transcriptional regulatory network and gaining insights into the molecular mechanisms governing heart development.

SONOGRAPHIC EVALUATION OF ANTERIOR ABDOMINAL WALL MASS AND DIAGNOSTIC ACCURACY OF HIGH FREQUENCY ULTRASOUND.

2021 ◽  
pp. 51-52
Author(s):  
G. Gomathi
Keyword(s):  
Diagnostic Accuracy ◽  
Abdominal Wall ◽  
High Frequency ◽  
Anterior Abdominal Wall ◽  
Imaging Modality ◽  
Clinical Manifestations ◽  
High Frequency Ultrasound ◽  
Wall Mass ◽  
Frequency Ultrasound ◽  
Wall Lesions

Aim :Evaluation of anterior abdominal wall masses using high frequency ultrasound to nd out varoius causes of anterior abdominal wall mass and diagnostic accuracy of high frequency ultrasound. Materials and Methods: 50 patients with the clinical manifestations of various anterior abdominal wall lesions were included in this study. All patients were examined the anterior abdominal wall using 7.0-12.0 MHz high-frequency linear transducer with Color Doppler . Results: Incisional hernia was the predominant anterior abdominal wall lesions followed by ventral hernias, lipomas, and hematomas cases. Least common was scar endometriosis,anterior abdominal wall sarcoma. the high-resolution ultrasound had an overall 100% accuracy for abdominal wall lesions. Conclusion: High-frequency sonography is an accurate diagnostic imaging modality in anterior abdominal wall lesions and guide to management aspect.

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