scholarly journals Identification and Quantification of Aldose Reductase Inhibitory Flavonoids in Herbal Formulation and Extract of Gymnema sylvestre Using HPLC-PDA and LC-MS/MS

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Nanjappan Satheeshkumar ◽  
Saladi Shantikumar ◽  
Mopuri Komali
Keyword(s):  
Mass Spectrometry ◽  
Aldose Reductase ◽  
Chromatographic Separation ◽  
Gymnema Sylvestre ◽  
Herbal Supplements ◽  
Active Constituents ◽  
Polyherbal Formulation ◽  
Flight Mass Spectrometry ◽  
Herbal Formulation ◽  
Gradient Mode

Adulteration of herbal supplements is a major issue for many countries. A simple and reliable HPLC-PDA method was developed for quantification of aldose reductase inhibitory flavonoids rutin, quercetin, and kaempferol. The chromatographic separation was performed on a Fortis C18 column in gradient mode with detection at 267 nm. The presence of these markers was confirmed through the accurate m/z values and MS/MS data obtained using quadruple time of flight mass spectrometry (QTOF-MS). The proposed method was successfully applied to examine the amount of these active constituents in antiobese polyherbal formulation and plant extract of Gymnema sylvestre.

scholarly journals Liquid Chromatography/Quadrupole Time-of-Flight Mass Spectrometry for Identification of In Vitro and In Vivo Metabolites of Bornyl Gallate in Rats

2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
Wei Lan ◽  
Liujiao Bian ◽  
Xinfeng Zhao ◽  
Pu Jia ◽  
Xue Meng ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Chromatographic Separation ◽  
Gradient Elution ◽  
Drug Candidate ◽  
Rat Urine ◽  
Molecular Weights ◽  
Flight Mass Spectrometry ◽  
Vessel Growth

Bornyl gallate (BG) is a potential drug candidate synthesized by the reaction of two natural products, gallic acid and borneol. Previous studies have strongly suggested that BG is worthy of further investigation due to antioxidant, antiatherosclerosis activities, and obvious activity of stimulating intersegmental vessel growth in zebrafish. This work was designed to elucidate the metabolic profile of BG through analyzing its metabolites in vitro and in vivo by a chromatographic separation coupled with a mass spectrometry. The metabolites of BG were characterized from the rat liver microsome incubation solution, as well as rat urine and plasma after oral administration. Chromatographic separation was performed on an Agilent TC-C18column (250 mm × 4.6 mm, 5 μm) with gradient elution using methanol and water containing 0.2% (V : V) formic acid as the mobile phase. Metabolites identification involved analyzing the retention behaviors, changes of molecular weights and MS/MS fragment patterns of BG and the metabolites. Five compounds were identified as isomers of hydroxylated BG metabolites in vitro. The major metabolites of BG in rat urine and plasma proved to be BG-O-glucuronide and O-methyl BG-O-glucuronide. The proposed method confirmed to be a reliable and sensitive alternative for characterizing metabolic pathways of BG.

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