scholarly journals Evaluation of Antibody Response to Various Developmental Stage Specific Somatic Antigens ofParamphistomum epiclitumin Goats

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Jyoti ◽  
A. Prasad ◽  
Nirbhay Kumar Singh
Keyword(s):  
Early Diagnosis ◽  
Antibody Response ◽  
Developmental Stage ◽  
Statistical Significance ◽  
Polyacrylamide Gel Electrophoresis ◽  
Electrophoretic Analysis ◽  
Igg Antibody ◽  
Native Polyacrylamide Gel Electrophoresis ◽  
Paramphistomum Epiclitum ◽  
Somatic Antigens

Electrophoretic analysis of various developmental stage specific somatic antigens ofParamphistomum epiclitum(Digenea: Paramphistomidae), namely, metacercariae (McAg), immature intestinal flukes (ImIAg), immature ruminal flukes (ImRAg), and adult flukes (AAg), was done by native polyacrylamide gel electrophoresis. Result revealed presence of 3 (range 15.2–40.3 kDa), 13 (9.3–121.2 kDa), 14 (9.3–169.3 kDa), and 15 (8.0–169.3 kDa) polypeptides in McAg, ImIAg, ImRAg, and AAg, respectively. With an aim to identify a suitable immunodiagnostic antigen for early diagnosis of amphistomosis, the IgG antibody response to various developmental stage antigens in goats experimentally infected with metacercariae ofP. epiclitumwas evaluated by ELISA. The highest OD values were recorded with ImIAg which ranged between 0.23 and 0.55 with a significant increase from the 2nd week till 8th week of infection with a peak at 6th week. The analysis of statistical significance using a one-way analysis of variance with multiple pair wise comparisons revealed that IgG response was significantly higher with all antigens (P<0.01) except McAg (P>0.05) with a maximum mean difference of 0.1838 in comparison to control with ImIAg, thus, indicating that ImIAg which could be further exploited for its potential is a candidate for immunodiagnostic antigen for early diagnosis of amphistomosis.

IgG antibody response to ES or somatic antigens of Dicrocoelium dendriticum (Trematoda) in experimentally infected sheep

2000 ◽  
Vol 86 (6) ◽  
pp. 472-479 ◽  
Author(s):  
C. González-Lanza ◽  
M. Y. Manga-González ◽  
R. Campo ◽  
P. Del-Pozo ◽  
H. Sandoval ◽  
...  
Keyword(s):  
Antibody Response ◽  
Infected Sheep ◽  
Igg Antibody ◽  
Dicrocoelium Dendriticum ◽  
Somatic Antigens

scholarly journals Antibody response among COVID-19 patient with and without re-detectable positive RT-PCR results in convalescent phases

2021 ◽  
Author(s):  
Jing Peng ◽  
Zhi-Yong Liu ◽  
Xiaojuan YU ◽  
Xiao-Yan Chen ◽  
Kai Zhang ◽  
...  
Keyword(s):  
Antibody Response ◽  
Statistical Significance ◽  
Rt Pcr ◽  
Igg Antibody ◽  
Blood Samples ◽  
Illness Onset ◽  
Antibody Titers ◽  
Iga Antibody ◽  
Antibody Kinetics ◽  
Kinetics Of

Abstract Objective: To analyze the dynamic of total, IgA, IgM and IgG antibody of the confirmed COVID-19 patients during convalescent phases to understand the kinetics of antibody response among recovered patients.Methods: From March 4 to April 29, 2020, a total of 143 recovered COVID-19 patients with clear date of illness onset available were enrolled in this study. Nasopharyngeal and anal swabs were collected for SARS-CoV-2 RNA testing. Blood samples were collected for antibodies testing. Results: A total of 275 blood samples up to 96 days after illness onset were collected from 143 recovered patients. High titers of total and IgG antibodies continued to persist for over 3 months, with 100% and 99.3% patients remaining positive for total and IgG antibody. IgM antibody declined rapidly with a median time to seronegative at 67 (95%CI: 59, 75) days after illness onset. Around 25% patients were seronegative for IgA antibody at month 3 after illness onset. No statistical significance difference was founded in the antibody kinetics between patients with and without re-detectable positive RT-PCR results during in convalescent phases. Conclusion: Similar high antibody titers of total and IgG antibody continued to persist for over 3 months among recovered COVID-19 patients with and without re-detectable positive RT-PCR results.

scholarly journals The Native Monomer of Bacillus Pumilus Ribonuclease Does Not Exist Extracellularly

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Olga Ilinskaya ◽  
Vera Ulyanova ◽  
Irina Lisevich ◽  
Elena Dudkina ◽  
Nataliya Zakharchenko ◽  
...  
Keyword(s):  
Conformational Changes ◽  
Protein Concentration ◽  
Bacillus Pumilus ◽  
Polyacrylamide Gel Electrophoresis ◽  
Culture Fluid ◽  
Size Exclusion ◽  
High Protein Concentration ◽  
Exclusion Chromatography ◽  
Native Polyacrylamide Gel Electrophoresis ◽  
Hypochromic Effect

Supported by crystallography studies, secreted ribonuclease of Bacillus pumilus (binase) has long been considered to be monomeric in form. Recent evidence obtained using native polyacrylamide gel electrophoresis and size-exclusion chromatography suggests that binase is in fact dimeric. To eliminate ambiguity and contradictions in the data we have measured conformational changes, hypochromic effect, and hydrodynamic radius of binase. The immutability of binase secondary structure upon transition from low to high protein concentration was registered, suggesting the binase dimerization immediately after translocation through the cell membrane and leading to detection of binase dimers only in the culture fluid regardless of ribonuclease concentration. Our results made it necessary to take a fresh look at the binase stability and cytotoxicity towards virus-infected or tumor cells.

scholarly journals Trichinella britovihuman infection in Spain : antibody response to surface, excretory/secretory and somatic antigens

Parasite ◽  
2003 ◽  
Vol 10 (2) ◽  
pp. 159-164 ◽  
Author(s):  
M. Rodríguez-Osorio ◽  
V. Gómez-Garcia ◽  
R. Benito ◽  
J. Gil
Keyword(s):  
Antibody Response ◽  
Somatic Antigens

scholarly journals New Autoantibody Specificities in Systemic Sclerosis and Very Early Systemic Sclerosis

Antibodies ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 12
Author(s):  
Roberto Lande ◽  
Raffaella Palazzo ◽  
Anna Mennella ◽  
Immacolata Pietraforte ◽  
Marius Cadar ◽  
...  
Keyword(s):  
Systemic Sclerosis ◽  
Early Diagnosis ◽  
Antibody Response ◽  
Allelic Variant ◽  
The Other ◽  
Autoantibody Response ◽  
Autoimmune Condition ◽  
Early Systemic Sclerosis ◽  
Systemic Sclerosis Patient

Chemokine (C-X-C motif) ligand 4 (CXCL4) is a biomarker of unfavorable prognosis in Systemic Sclerosis (SSc), a potentially severe autoimmune condition, characterized by vasculitis, fibrosis and interferon (IFN)-I-signature. We recently reported that autoantibodies to CXCL4 circulate in SSc patients and correlate with IFN-α. Here, we used shorter versions of CXCL4 and CXCL4-L1, the CXCL4 non-allelic variant, to search for autoantibodies exclusively reacting to one or the other CXCL4 form. Moreover, to address whether anti-CXCL4/CXCL4-L1 antibodies were present before SSc onset and predicted SSc-progression, we longitudinally studied two VEDOSS (Very Early Diagnosis of Systemic Sclerosis) patient cohorts, separating SSc-progressors from SSc-non-progressors. We found that anti-CXCL4-specific autoantibodies were present in both SSc and VEDOSS patients (both SSc-progressors and SSc-non-progressors). Anti-CXCL4-L1-specific autoantibodies were especially detected in long-standing SSc (lsSSc). Anti-CXCL4/CXCL4-L1 antibodies correlated with IFN-α and with specific SSc-skin features but only in lsSSc and not in early SSc (eaSSc) or VEDOSS. Thus, a broader antibody response, with reactivity spreading to CXCL4-L1, is characteristic of lsSSc. The early anti-CXCL4 autoantibody response seems qualitatively different from, and likely less pathogenic than, that observed in advanced SSc. Lastly, we confirm that anti-CXCL4 autoantibodies are SSc-biomarkers and uncover that also CXCL4-L1 becomes an autoantigen in lsSSc.

scholarly journals Identity and Substrate Specificity of Reductive Dehalogenases Expressed in Dehalococcoides-Containing Enrichment Cultures Maintained on Different Chlorinated Ethenes

2015 ◽  
Vol 81 (14) ◽  
pp. 4626-4633 ◽  
Author(s):  
Xiaoming Liang ◽  
Olivia Molenda ◽  
Shuiquan Tang ◽  
Elizabeth A. Edwards
Keyword(s):  
Specific Activity ◽  
Enrichment Culture ◽  
Polyacrylamide Gel Electrophoresis ◽  
Peptide Sequencing ◽  
Enzyme Assays ◽  
Content Type ◽  
Substrate Preferences ◽  
Native Polyacrylamide Gel Electrophoresis ◽  
History Of ◽  
Different Strains

ABSTRACTMany reductive dehalogenases (RDases) have been identified in organohalide-respiring microorganisms, and yet their substrates, specific activities, and conditions for expression are not well understood. We tested whether RDase expression varied depending on the substrate-exposure history of reductive dechlorinating communities. For this purpose, we used the enrichment culture KB-1 maintained on trichloroethene (TCE), as well as subcultures maintained on the intermediatescis-dichloroethene (cDCE) and vinyl chloride (VC). KB-1 contains a TCE-to-cDCE dechlorinatingGeobacterand severalDehalococcoidesstrains that together harbor many of the known chloroethene reductases. Expressed RDases were identified using blue native polyacrylamide gel electrophoresis, enzyme assays in gel slices, and peptide sequencing. As anticipated but never previously quantified, the RDase fromGeobacterwas only detected transiently at the beginning of TCE dechlorination. TheDehalococcoidesRDase VcrA and smaller amounts of TceA were expressed in the parent KB-1 culture during complete dechlorination of TCE to ethene regardless of time point or amended substrate. TheDehalococcoidesRDase BvcA was only detected in enrichments maintained on cDCE as growth substrates, in roughly equal abundance to VcrA. Only VcrA was detected in subcultures enriched on VC. Enzyme assays revealed that 1,1-DCE, a substrate not used for culture enrichment, afforded the highest specific activity.trans-DCE was substantially dechlorinated only by extracts from cDCE enrichments expressing BvcA. RDase gene distribution indicated enrichment of different strains ofDehalococcoidesas a function of electron acceptor TCE, cDCE, or VC. Each chloroethene reductase has distinct substrate preferences leading to strain selection in mixed communities.

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