scholarly journals Hematology of Wild Caught Dubois’s Tree FrogPolypedates teraiensis, Dubois, 1986 (Anura: Rhacophoridae)

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Madhusmita Das ◽  
Pravati Kumari Mahapatra
Keyword(s):  
Blood Cell ◽  
Blood Serum ◽  
Blood Cells ◽  
Hematological Parameters ◽  
Hemoglobin Concentration ◽  
Mean Corpuscular Hemoglobin ◽  
Reference Ranges ◽  
Corpuscular Hemoglobin ◽  
Differential Leukocyte Count ◽  
Rbc Count

Blood was analyzed from eighty (forty males and forty females) adult individuals ofPolypedates teraiensisto establish reference ranges for its hematological and serum biochemical parameters. The peripheral blood cells were differentiated as erythrocytes, lymphocytes, eosinophils, neutrophils, monocytes, basophils, and thrombocytes, with similar morphology to other anurans. Morphology of blood cells did not vary according to sex. The hematological investigations included morphology and morphometry of erythrocytes, morphometry of leucocytes, packed cell volume (PCV), hemoglobin content (Hb), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), erythrocyte or red blood cell (RBC) count, leukocyte or white blood cell (WBC) count, differential leukocyte count, and neutrophil to lymphocyte (N/L) ratio. Besides, protein, cholesterol, glucose, urea, uric acid, and creatinine content of blood serum were assayed. Hematological parameters that differed significantly between sexes were RBC count, length and breadth of RBC, neutrophil %, N/L ratio, area occupied by basophils, and diameter of large lymphocyte and eosinophils. The level of glucose, urea, and creatinine in blood serum also significantly differed between sexes.

scholarly journals Hematological parameters in Nile Tilápia, Oreochromis niloticus exposed to sub-letal concentrations of mercury

2007 ◽  
Vol 50 (4) ◽  
pp. 619-626 ◽  
Author(s):  
Nilton Massuo Ishikawa ◽  
Maria José Tavares Ranzani-Paiva ◽  
Julio Vicente Lombardi ◽  
Cláudia Maris Ferreira
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
Oreochromis Niloticus ◽  
Hematological Parameters ◽  
Hemoglobin Concentration ◽  
Control Group ◽  
Mean Corpuscular Hemoglobin ◽  
Blood Cell Count ◽  
Corpuscular Hemoglobin ◽  
Set Up

Mercury toxicity in tilapia, Oreochromis niloticus, (Linnaeus, 1758) was investigated by the hematological parameters after long-term (14 days) exposure to various Hg concentrations (0.02, 0.002, 0.0002mg/L Hg). Test groups were set up with three replicates for each concentration, plus the control group. Blood samples were collected from six individuals for each concentration at 0, 3, 7, 10 and 14 days of exposure. The hematological parameters analyzed were: total red blood cell count (RBC), hemoglobin concentration (Hb), hematocrit (Ht), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), total white blood cell count (WBC) and differential leukocyte counts and total thrombocyte count (Tr). There were no significant differences among the mean hematological values at the different Hg concentrations indicating that Hg at the concentrations studied was not toxic to tilapia.

scholarly journals Hematological parameters of Iranian cichlid Iranocichla hormuzensis: Coad, 1982 (Perciformes) in Mehran River

2012 ◽  
Vol 84 (4) ◽  
pp. 943-949 ◽  
Author(s):  
Ehsan Daneshvar ◽  
Mahsa Y. Ardestani ◽  
Salar Dorafshan ◽  
Mauricio L. Martins
Keyword(s):  
Blood Cells ◽  
Mean Corpuscular Volume ◽  
High Efficiency ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Hemoglobin Concentration ◽  
Mean Corpuscular Hemoglobin ◽  
Mean Corpuscular Hemoglobin Concentration ◽  
Corpuscular Hemoglobin ◽  
Food Fish

This study describes the hematological parameters in Iranocichla hormuzensis, an Iranian freshwater cichlid important as ornamental and food fish. Forty fish were captured with seine net at Mehran river Hormozgan province, Iran. Blood was used to determine the total counts of red blood cells (RBC) and white blood cells (WBC), hematocrit, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC) and morphometric data of erythrocytes. The Iranian fish showed lower RBC and WBC values than the other cichlids (Oreochromis niloticus, O. aureus, O. mossambicus, O. hybrid, Cichlasoma dimerus and Cichla monoculus). Hematocrit did not vary among the species, but MCV, MCH and MCHC in I. hormuzensis were higher than those for O. niloticus, O. aureus, O. hybrid, C. dimerus and C. monoculus. These differences may be related to different life habit of fish. This study suggests that I. hormuzensis is well acclimated to the environment being the first report for its hematology. It is also suggested high efficiency in oxygen transportation, and an efficient inflow of oxygen by the gills, indicating the welfare of fish on this environment.

scholarly journals Effect of ketorolac administration post bone cavitation surgery on some hematological parameters

2017 ◽  
Vol 41 (1) ◽  
pp. 169-177
Author(s):  
Raffal A. Omar
Keyword(s):  
Blood Cells ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Hemoglobin Concentration ◽  
Treated Group ◽  
Control Group ◽  
Mean Corpuscular Hemoglobin ◽  
Mean Corpuscular Hemoglobin Concentration ◽  
Corpuscular Hemoglobin ◽  
Post Operation

     This study was designed to determine the effect of ketorolac on some hematological parameters post cavitation surgery in domestic rabbits. Ninety-six adult domestic local breed male rabbits were used in this study, weighing (1.23 ± 0.33) kg. They were divided randomly into two groups in the first one (n= 48/group) which represented control group, while the second one (n=48/group) represented the treated group (Ketorolac group). All rabbits in both groups undergoes surgical operation (bone cavitation in femur bone) under general anesthesia. In the Ketorolac group, animals were treated by using 30 mg/kg of Ketorolac directly post-operation and continued daily for 5 days, while in Control group no treatment was given postoperatively. The hematological parameters were recorded which include white blood cells count; red blood cells count; hemoglobin; packed cell volume; mean corpuscular hemoglobin and mean corpuscular hemoglobin concentration; mean corpuscular volume and platelets at the period of (3, 5, 7, 14, 21, 28, 35, and 42) days post operation were estimated. Results concerning hematological values showed no significant differences in packed cell volume values between control and treated group and within each group, while haemoglobin values showed a significant decrease at period 7 and 14 days between control and treated group. Within control group the significant decrease were noticed clearly at period of 7 days. Data concerning mean corpuscular hemoglobin values within control group showed significant decrease were noticed at 5 day, but within treated group, there were no significant differences. mean corpuscular hemoglobin concentration values showed significant decrease ate period 3; 5 and 21 days between control group and treated group respectively. Within control group the significant decrease were noticed obviously at 5 days while within treated group the significant decrease were noticed at 35 days. mean corpuscular volume values showed significant increase at 3 and 5 days period between control and treated groups. Platelets counts within control group showed significant increase at period of 14 days, within treated group the significant increase were recorded at 42 days. Red blood cells showed significant decrease between control and treated groups, while within control group, the significant decrease were at 7 days. White blood cells count within treated group showed significant increase recorded at 21 days. Differential White blood cells count concerning Lymphocytes cells within control group the significant increase were recorded at 42 days  while the significant decrease were noticed at 21 days, but within treated group the significant increase were recorded at 35 days  and significant decrease were noticed at 14 days. Neutrophils counts showed significant decrease at periods 3, 14, 28 and 42 days between control and treated groups respectively; while within control group there were significant decrease at 3 days, but within treated group the significant decrease were noticed at 42 days. Monocytes cells showed significant increase at 35 days between control and treated groups, within control group there were significant increase at 35 days, while within treated group the significant increase recorded at 7 days.

Effect of gender on hematological parameters in growing Ouled Djellal sheep of semi arid zones of Northeastern Algeria

2021 ◽  
Vol 11 (4) ◽  
pp. 407-417
Author(s):  
Zerari Aicha ◽  
Mahdi Djahida ◽  
Allaoua Sofia Amel ◽  
Rouar Salim
Keyword(s):  
Blood Cells ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Hemoglobin Concentration ◽  
Mean Corpuscular Hemoglobin ◽  
Arid Zones ◽  
Mean Corpuscular Hemoglobin Concentration ◽  
Corpuscular Hemoglobin ◽  
Mean Values ◽  
Ewe Lambs

Ouled Djellal sheep is the most dominant breed in Algeria due to its adapta-tion to harsh steppe environment. Hematological parameters are good indi-cators of animal health, but their values are breed-specific, and they are affected by age and gender. Therefore, this study aimed to determine the hematological profile of healthy Ouled Djellal lambs and ewe lambs reared traditionally under semi-arid zones of Algeria during the growing peri-od. Blood samples were taken from twenty-four Ouled Djellal lambs (12 males, 12 females) at 42, 63, 84 and 105 days of age to evaluate the count of White Blood Cells, Lymphocyte, Monocyte, Granulocyte, Red Blood Cell and total Platelet, the percentage of Lymphocyte, Monocyte and Granulocyte, Hemoglobin concentration, Hematocrit, Mean Corpuscular Volume, Mean Corpuscular Hemoglobin and Mean Corpuscular Hemoglobin Concentration by usual known methods. Both male and female lambs did not show signifi-cant variations in mean values of White Blood Cells, Lymphocyte count, Red Blood Cells, Hemoglobin, Mean Corpuscular Hemoglobin concentration, per-centage of Monocyte, Monocyte count, Gran percentage and Granulocyte count from 42ͭ ͪ day to 105ͭ ͪ days. Mean values of percentage of Lymphocyte, Hematocrite in lambs, Mean Corpuscular Hemoglobin in ewe lambs, and Mean Corpuscular Volume, Platelets levels in both lambs and ewe lambs indicated also variations during this period of growth. Significant Pearson's correlations were found between some hematological parameters in Ouled Djellal lambs and/or ewe lambs. Therefore, these findings provide usual val-ues, which can be considered for assessing the physiological, nutritional and health status of these animals.

scholarly journals Changes in hematological parameters and gill morphology in common carp exposed to sub-lethal concentrations of Malathion

2016 ◽  
Vol 2 (3) ◽  
pp. 370-378 ◽  
Author(s):  
Sadia Sharmin ◽  
Md Abdus Salam ◽  
Farhana Haque ◽  
Md Sadiqul Islam ◽  
Md Shahjahan
Keyword(s):  
Common Carp ◽  
Blood Cells ◽  
Morphological Changes ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Hemoglobin Concentration ◽  
Blood Parameters ◽  
Mean Corpuscular Hemoglobin ◽  
Agricultural Field ◽  
Corpuscular Hemoglobin

Malathion is an organophosphorous insecticide widely used in the agricultural field in Bangladesh and reach in the aquatic environment through rain wash. In the present study, we examined the effects of Malathion on hematological parameters and gills morphology in common carp exposed to two sub-lethal concentrations (1.5 and 3.0 mg/L) for a period of 192h (8 days). For hematological parameters fish were sacrificed at 24, 48, 72, and 96h after start of exposure. Gills were collected at 192h after start of exposure. The blood glucose level was significantly elevated with increasing the concentration of Malathion. Red blood cells (RBCs), hemoglobin (Hb) and hematocrit (Hct) values were significantly decreased after the exposure of Malathion. Significant increments were observed in case of the mean corpuscular volume (MCV) and mean corpuscular hemoglobin concentration (MCHC), while no distinct changes were noted for mean corpuscular hemoglobin (MCH). Interestingly, white blood cells (WBCs) count was significantly increased with toxicity of Malathion. Several morphological changes, such as telangiectasia, blood lamellar congestion, hypertrophy of filaments, lamellar fusion were observed in the gills of fish exposed to Malathion. The present study revealed that the insecticide had adverse effects on various blood parameters and gills morphology in common carp. Thus, the use of insecticide in the agriculture field may be a threat to fauna and flora of the aquatic environment.Asian J. Med. Biol. Res. September 2016, 2(3): 370-378

scholarly journals Hematological profile of wild-captured Indian Flying Fox (Pteropus giganteus) in Bangladesh

2016 ◽  
pp. 12-17
Author(s):  
MB Hossain ◽  
MN Islam ◽  
MG Yasin ◽  
MM Hassan ◽  
SKMA Islam ◽  
...  
Keyword(s):  
Blood Cell ◽  
Body Condition Score ◽  
Hematological Parameters ◽  
Blood Collection ◽  
Mean Corpuscular Hemoglobin ◽  
Health State ◽  
Corpuscular Hemoglobin ◽  
Distribution Width ◽  
Hematological Profile ◽  
Rbc Count

The current study was conducted to examine hematological parameters of large frugivorous bats under the class mammalian. Ninety (90) clinically healthy large frugivorous bats were captured and anesthetized for blood collection from brachial vein. Captured bats were divided into three groups’ based on different criteria such as sex, age and body condition score. Red blood cell (RBC) count, hemoglobin (Hb), hematocrits (Hct), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration(MCHC), red cell distribution width (RDWs), white blood cell (WBC) count, platelets (PLT), mean platelet volume (MPV), platelet distribution width (PDW) were determined by automated hematology analyzer and erythrocyte sedimentation rate (ESR) was determined manually. RBC and MCV values were found significantly (p < 0.05) higher in male bats. On the other hand, the RBC count, HCT, Hb, RDWs and PLT were significantly (p < 0.05) higher in adults than in juveniles bats. Among the health state RBC, HCT and PLT values were significantly (p < 0.05) higher in good-health bats. However, some values were varied in different groups, but they were not statistically significant (p >0.05). The values from this study can be used to create reference of hematological profile of bat found in Indian subcontinent, and can make up the first comprehensive hematological study for this highly endangered species.International Journal of Natural Sciences (2013), 3(1-4) 12-17

scholarly journals Reference Ranges for Hematological and Biochemical Profile of Martina Franca Donkeys

2020 ◽  
Vol 7 ◽  
Author(s):  
Francesca Trimboli ◽  
Ippolito De Amicis ◽  
Antonio Di Loria ◽  
Carlotta Ceniti ◽  
Augusto Carluccio
Keyword(s):  
Blood Cell ◽  
Hematological Parameters ◽  
Hemoglobin Concentration ◽  
Conservation Strategies ◽  
Total Serum ◽  
Mean Corpuscular Hemoglobin ◽  
Reference Ranges ◽  
Platelet Volume ◽  
Distribution Width ◽  
Glutamyl Transferase

The Martina Franca donkey (MFd) is one of the largest Italian donkey breeds, considered as endangered breed. To support the conservation strategies, knowledge about the physiologic hematological parameters of MFds is needed. The aims of the study were to determine reference value for hematological and major serum parameters in a population of healthy MFds and to estimate the influence of age on these parameters. Eighty-one clinically healthy MFds (17 males and 64 females) in different ages were enrolled: group A (foals, n° 16, animals &lt; 1 year old) group B (young animals, n° 36, from 1 to 3 years old), and group C (adult animals, n° 29, over 3 years old). Red blood cell count (RBC); hematocrit value (HCT); hemoglobin concentration (HGB); mean corpuscular volume (MCV); mean corpuscular hemoglobin (MCH); hemoglobin concentration distribution width (HDW); RBC distribution width (RDW); total white blood cell (WBC); WBC differential count for neutrophils, lymphocytes, monocytes, eosinophils and basophils, and platelets (PLT); mean platelet volume (MPV); platelet volume distribution width; and plateletcrit (PCT) were analyzed. The biochemistry panel included aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), gamma glutamyl transferase (GGT), total serum protein (TP), albumin (ALB), cholesterol (CHOL), triglyceride (TGL), blood urea nitrogen (BUN), creatinine (CREA), glucose (GLU), Ca2+, phosphorus, Na+, Cl−, K+, and Mg2+. The effect of age on hematological parameters was investigated using one-way ANOVA test. Age of donkeys does not influence total WBC, HGB, HCT, platelet count and MPV, and PCT (P &gt; 0.05). Some leukocyte populations such as eosinophils, monocytes, and basophils showed age-linked variations (P &lt; 0.05). RBC count, RDW, and HDW decrease with age whereas MCV and MCH increase. Na+, K+, Cl−, Ca2+, phosphorus, ALP, GGT, CREA, GLUC, and CHOL decrease with age (P &lt; 0.05), while AST and TP showed an increase with aging (P &lt; 0.05). ALB reaches the lowest values in young donkeys and returns to values of foals in older animals (P &lt; 0.05). Finally, a difference among groups for BUN and TGL was not found (P &lt; 0.05). The results suggest how even for the MFd breed, age is a variable that affects different hematological and biochemical parameters. Compared to other donkey and horses, the MFd breed showed some differences that clinicians involved during conservation strategies need to be consider.

scholarly journals Factors Affecting the Hematological Parameters in Different Goat Breeds from Italy

2016 ◽  
Vol 16 (3) ◽  
pp. 743-757 ◽  
Author(s):  
Francesca Arfuso ◽  
Francesco Fazio ◽  
Maria Rizzo ◽  
Simona Marafioti ◽  
Elisa Zanghì ◽  
...  
Keyword(s):  
Blood Cell ◽  
Reference Values ◽  
Hematological Parameters ◽  
Animal Health ◽  
Laboratory Data ◽  
Hemoglobin Concentration ◽  
Mean Corpuscular Hemoglobin ◽  
Corpuscular Hemoglobin ◽  
Factors Affecting ◽  
Management And Conservation

AbstractHematological parameters in healthy goats show several variations in relation to breed, age, reproductive status, environmental factors and management conditions. Therefore, there is the need to investigate these factors and how they affect the animal’s hematology. The aims of this study were to establish hematological reference values of five Italian goat breeds at different age classes (1–2, 3–4 and over 5 years) considered in autumn and to evaluate the effects of some factors (breed, age and environmental conditions). Ninety-six dried female Aspromontana goats, 102 Girgentana goats, 99 Messinese goats, 96 Maltese goats and 108 Argentata dell’Etna goats, clinically healthy, were used. Blood samples were collected from each animal and analyzed for Red Blood Cell (RBC), White Blood Cell (WBC), Hemoglobin concentration (Hb), Hematocrit (Hct), Mean Corpuscular Volume (MCV), Mean Corpuscular Hemoglobin (MCH), Mean Corpuscular Hemoglobin Concentration (MCHC) and Platelets (PLT). Statistical analysis showed the effect of breed (P<0.05) on all studied parameters and the effect of age (P<0.05) on: RBC and Hb in Messinese goats; Hct in Girgentana, Maltese and Argentata dell’Etna goats; MCV and MCHC in Argentata dell’Etna goats; WBC in Aspromontana goats; and PLT in Girgentana and Messinese goats. The findings of the present study may serve as reference values for hematology of Italian goat breeds studied in autumn which could help veterinarians to interpret laboratory data appropriately and to monitor animal health status in order to improve the management and conservation of these breeds.

Hematological values in bradypus variegatus (Schinz, 1825) from creatories of the Belém metropolitan region

2019 ◽  
Vol 10 (5) ◽  
pp. 82-88 ◽  
Author(s):  
Gilmar Rodrigues Galvão ◽  
Elane Guerreiro Giese ◽  
Gilvando Rodrigues Galvão ◽  
Luiz Fernando Moraes Moreira ◽  
Maridelzira Betânia Moraes David ◽  
...  
Keyword(s):  
Blood Cells ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Hemoglobin Concentration ◽  
Cell Types ◽  
Metropolitan Region ◽  
Corpuscular Hemoglobin ◽  
Differential Leukocyte Count ◽  
Bradypus Variegatus ◽  
Hematological Values

The blood tissue is made up of three main cell types: erythrocytes (red blood cells), leukocytes (white blood cells) and platelets (thrombocytes), suspended in a liquid phase named plasma. In addition to the cells, blood is composed of proteins, inorganic and organic substances, hormones and various components. The captive condition can also predispose the animal to a number of conditions, making it necessary to carry out laboratory tests for the biomonitoring of the animals. Among these tests, the complete hemogram stands out. This is done to assess the general health, guiding and deepening the nature of pathophysiological situations besides its utility as an aid to diagnosis and prognosis of certain diseases. The objective of this study is to describe the hematological values of brown-throated sloth (Bradypus variegatus). Hence, eleven adult animals of both sexes (seven males and four females) at appropriate health conditions were used in the experiment. Blood was collected from the cephalic vein and analyzed through veterinary hematology counter. The evaluate hematological parameters included total count of erythrocytes and leukocytes, hematocrit, hemoglobin concentration, average corpuscular volume, average corpuscular hemoglobin, average corpuscular hemoglobin concentration differential leukocyte count and platelet count. In general, the values did not differ from few studies in the literature. However, the score of platelets was greater in males than in females. The results achieved in the study can serve as a basis in determination and interpretation as hematologic references to brown-throated sloths in Para Amazon.

Pesticides Residues in Blood Serum and Their Adverse Effects on Multi- Biomarkers in Occupationally Exposed Greenhouse Workers: A Cross-Sectional Study in Hamadan City, Iran

2021 ◽  
Author(s):  
Mostafa Leili ◽  
Abolfazl Ghafouri Khosrowshahi ◽  
Jalal Poorolajal ◽  
Fateme Samiee ◽  
Mohammad Taghi Samadi ◽  
...  
Keyword(s):  
Blood Serum ◽  
Crop Production ◽  
Hematological Parameters ◽  
Severe Form ◽  
Mean Corpuscular Hemoglobin ◽  
Adult Males ◽  
Corpuscular Hemoglobin ◽  
Cross Sectional ◽  
Pesticides Residues ◽  
Greenhouse Workers

Abstract The cultivation of greenhouse crops is the most severe form of crop production in Iran, and vast quantities of unreasonable pesticides are being utilized to control pests. The residual level of blood pesticides and its correlation with multi- biomarkers were determined to evaluate the adverse health consequences on greenhouse workers in the Hamdan city of Iran. Participants were 180 adult males, including 90 greenhouse workers exposed to pesticides and 90 control individuals. The residue analyses revealed that 64 out of the 90 workers had residues of dichlorvos, diazinon, and chlorpyrifos in their blood serum; and 56.25% out of these workers were exposed for > 15 years period. The residues of all three pesticides were higher than the no observable adverse effect level (NOAEL) in all the exposure duration categories. Comparisons of hematological parameters showed that mean monocyte (P < 0.001), lymphocyte (P < 0.001) and platelet counts (P = 0.001), mean platelet volume (P = 0.002), and plateletcrit (PCT) (P < 0.001) were significantly higher in workers than in controls, whereas hemoglobin (HB) (P = 0.028), mean corpuscular hemoglobin (MCH), and mean corpuscular hemoglobin concentrations (MCHC) (P < 0.001) were lower in workers than controls. These results show that exposure to different pesticides was associated with alterations in some biochemical and hematological parameters. Prevention and intervention programs can be effective in eliminating pesticide-related alterations among agricultural workers.

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