scholarly journals Overexpression of a Novel Component Induces HAK5 and Enhances Growth in Arabidopsis

ISRN Botany ◽  
2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Eri Adams ◽  
Celine Diaz ◽  
Minami Matsui ◽  
Ryoung Shin
Keyword(s):  
Arabidopsis Thaliana ◽  
Salt Stress ◽  
Adaptive Response ◽  
Screening Method ◽  
Primary Root ◽  
Nutrient Deficiency ◽  
Potassium Deficiency ◽  
Low Potassium ◽  
Primary Root Growth ◽  
Gene Hunting

Plants have developed mechanisms to adapt to the potassium deficient conditions over the years. In Arabidopsis thaliana, expression of a potassium transporter HAK5 is induced in low potassium conditions as an adaptive response to nutrient deficiency. In order to understand the mechanism in which HAK5 is regulated, the full-length cDNA overexpressor gene hunting system was employed as a screening method. Of 40 genes recovered, At4g18280 was found to be dramatically induced in response to potassium-deficiency and salt stress. Plants overexpressing this gene showed higher HAK5 expression and enhanced growth. These plants were also less sensitive to potassium-deficiency in terms of primary root growth. Taken together, these data suggest that this novel component, At4g18280, contributes to regulation of HAK5 and, consequently, tolerance to potassium-deficiency in plants.

scholarly journals 6-SFT, a Protein from Leymus mollis, Positively Regulates Salinity Tolerance and Enhances Fructan Levels in Arabidopsis thaliana

2019 ◽  
Vol 20 (11) ◽  
pp. 2691
Author(s):  
Mao Li ◽  
Xiaolan He ◽  
Dongdong Hao ◽  
Jun Wu ◽  
Jixin Zhao ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Salt Stress ◽  
Transgenic Plants ◽  
Stress Tolerance ◽  
Abiotic Stress Tolerance ◽  
Primary Root ◽  
Salt Stress Tolerance ◽  
Primary Root Growth ◽  
Rapid Amplification ◽  
Leymus Mollis

Fructans play vital roles in abiotic stress tolerance in plants. In this study, we isolated the sucrose:6-fructosyltransferase gene, which is involved in the synthesis of fructans, from Leymus mollis by rapid amplification of cDNA ends. The Lm-6-SFT gene was introduced into Arabidopsis thaliana cv. Columbia by Agrobacterium-mediated transformation. The transgenic plants were evaluated under salt stress conditions. The results showed that the expression of Lm-6-SFT was significantly induced by light, abscisic acid (ABA), salicylic acid (SA), and salt treatment in L. mollis plants. Overexpression of Lm-6-SFT in Arabidopsis promoted seed germination and primary root growth during the early vegetative growth stage under salt stress. We also found that the transgenic plants expressing Lm-6-SFT had increased proline and fructan levels. β-Glucuronidase staining and promoter analysis indicated that the promoter of Lm-6-SFT was regulated by light, ABA, and salt stress. Quantitative PCR suggested that overexpression of Lm-6-SFT could improve salt tolerance by interacting with the expression of some salt stress tolerance genes. Thus, we demonstrated that the Lm-6-SFT gene is a candidate gene that potentially confers salt stress tolerance to plants. Our study will aid the elucidation of the regulatory mechanism of 6-SFT genes in herb plants.

Apical meristem exhaustion during determinate primary root growth in the moots koom 1 mutant of Arabidopsis thaliana

Planta ◽  
2011 ◽  
Vol 234 (6) ◽  
pp. 1163-1177 ◽  
Author(s):  
Alejandra Hernández-Barrera ◽  
Yamel Ugartechea-Chirino ◽  
Svetlana Shishkova ◽  
Selene Napsucialy-Mendivil ◽  
Aleš Soukup ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Root Growth ◽  
Apical Meristem ◽  
Primary Root ◽  
Primary Root Growth

scholarly journals Exogenous Nitro-Oleic Acid Treatment Inhibits Primary Root Growth by Reducing the Mitosis in the Meristem in Arabidopsis thaliana

2020 ◽  
Vol 11 ◽  
Author(s):  
Luciano M. Di Fino ◽  
Ignacio Cerrudo ◽  
Sonia R. Salvatore ◽  
Francisco J. Schopfer ◽  
Carlos García-Mata ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Oleic Acid ◽  
Root Growth ◽  
Acid Treatment ◽  
Primary Root ◽  
Primary Root Growth

scholarly journals Nitrate Induction of Primary Root Growth Requires Cytokinin Signaling in Arabidopsis thaliana

2019 ◽  
Vol 61 (2) ◽  
pp. 342-352 ◽  
Author(s):  
Pamela A Naulin ◽  
Grace I Armijo ◽  
Andrea S Vega ◽  
Karem P Tamayo ◽  
Diana E Gras ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Cell Division ◽  
Root Growth ◽  
Primary Root ◽  
Growth Arrest ◽  
Root Tip ◽  
Wild Type ◽  
Cytokinin Signaling ◽  
Control Growth ◽  
Primary Root Growth

Abstract Nitrate can act as a potent signal to control growth and development in plants. In this study, we show that nitrate is able to stimulate primary root growth via increased meristem activity and cytokinin signaling. Cytokinin perception and biosynthesis mutants displayed shorter roots as compared with wild-type plants when grown with nitrate as the only nitrogen source. Histological analysis of the root tip revealed decreased cell division and elongation in the cytokinin receptor double mutant ahk2/ahk4 as compared with wild-type plants under a sufficient nitrate regime. Interestingly, a nitrate-dependent root growth arrest was observed between days 5 and 6 after sowing. Wild-type plants were able to recover from this growth arrest, while cytokinin signaling or biosynthesis mutants were not. Transcriptome analysis revealed significant changes in gene expression after, but not before, this transition in contrasting genotypes and nitrate regimes. We identified genes involved in both cell division and elongation as potentially important for primary root growth in response to nitrate. Our results provide evidence linking nitrate and cytokinin signaling for the control of primary root growth in Arabidopsis thaliana.

Ethylene mediates dichromate-induced inhibition of primary root growth by altering AUX1 expression and auxin accumulation in Arabidopsis thaliana

2018 ◽  
Vol 41 (6) ◽  
pp. 1453-1467 ◽  
Author(s):  
Abdul Wakeel ◽  
Imran Ali ◽  
Sakila Upreti ◽  
Azizullah Azizullah ◽  
Bohan Liu ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Root Growth ◽  
Primary Root ◽  
Primary Root Growth ◽  
Auxin Accumulation

scholarly journals Arabidopsis thaliana GH3.9 influences primary root growth

Planta ◽  
2007 ◽  
Vol 226 (1) ◽  
pp. 21-34 ◽  
Author(s):  
Sadaf Khan ◽  
Julie M. Stone
Keyword(s):  
Arabidopsis Thaliana ◽  
Root Growth ◽  
Primary Root ◽  
Primary Root Growth

Natural variation in primary root growth and K+ retention in roots of habanero pepper (Capsicum chinense) under salt stress

2016 ◽  
Vol 43 (12) ◽  
pp. 1114 ◽  
Author(s):  
Emanuel Bojórquez-Quintal ◽  
Nancy Ruiz-Lau ◽  
Ana Velarde-Buendía ◽  
Ileana Echevarría-Machado ◽  
Igor Pottosin ◽  
...  
Keyword(s):  
Salt Stress ◽  
Root Growth ◽  
Natural Variation ◽  
Primary Root ◽  
Capsicum Chinense ◽  
Pharmacological Agents ◽  
Habanero Pepper ◽  
Nacl Concentration ◽  
Primary Root Growth ◽  
Chichen Itza

In this work, we analysed the natural variation in mechanisms for protection against salt stress in pepper varieties (Capsicum chinense Jacq. cv. Rex, Chichen-Itza and Naranja and Capsicum annuum L. cv. Padron), considering primary root growth and viability of the post-stressed seedlings. NaCl-induced K+ and H+ efflux in roots was also studied by ion-selective microelectrodes under application of pharmacological agents. In these pepper varieties, the magnitude of the K+ leakage in the roots positively correlated with growth inhibition of the primary root in the presence of NaCl, with Rex variety showing a higher level of tolerance than Chichen-Itza. The K+ leakage and the activity of the H+ pump in the roots were dependent on the NaCl concentration. Pharmacological analysis indicated that the NaCl-induced K+ leakage was mediated by TEA+-sensitive KOR channels but not by NSCC channels. In addition, we present evidence for the possible participation of proline, and a Na+-insensitive HAK K+ transporter expressed in habanero pepper roots for maintaining K+ homeostasis under salt stress conditions.

TPST is involved in fructose regulation of primary root growth in Arabidopsis thaliana

2020 ◽  
Vol 103 (4-5) ◽  
pp. 511-525
Author(s):  
Yingli Zhong ◽  
Jiyong Xie ◽  
Suzhen Wen ◽  
Wenwu Wu ◽  
Li Tan ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Root Growth ◽  
Primary Root ◽  
Primary Root Growth
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