scholarly journals Simultaneous Heterotrophic Nitrification and Aerobic Denitrification byChryseobacteriumsp. R31 Isolated from Abattoir Wastewater

2014 ◽  
Vol 2014 ◽  
pp. 1-12 ◽  
Author(s):  
Pradyut Kundu ◽  
Arnab Pramanik ◽  
Arpita Dasgupta ◽  
Somnath Mukherjee ◽  
Joydeep Mukherjee
Keyword(s):  
Nitrogen Removal ◽  
Large Scale ◽  
Aerobic Denitrification ◽  
Utilization Rate ◽  
Close Relative ◽  
Yield Coefficient ◽  
Kinetic Coefficients ◽  
Nitrification And Denitrification ◽  
Monod Kinetic ◽  
Nitrate And Nitrite

A heterotrophic carbon utilizing microbe (R31) capable of simultaneous nitrification and denitrification (SND) was isolated from wastewater of an Indian slaughterhouse. From an initial COD value of 583.0 mg/L, 95.54% was removed whilst, from a startingNH4+-N concentration of 55.7 mg/L, 95.87% was removed after 48 h contact. The concentrations of the intermediates hydroxylamine, nitrite, and nitrate were low, thus ensuring nitrogen removal. Aerobic denitrification occurring during ammonium removal by R31 was confirmed by utilization of both nitrate and nitrite as nitrogen substrates. Glucose and succinate were superior while acetate and citrate were poor substrates for nitrogen removal. Molecular phylogenetic identification, supported by chemotaxonomic and physiological properties, assigned R31 as a close relative ofChryseobacterium haifense. TheNH4+-N utilization rate and growth of strain R31 were found to be higher at C/N = 10 in comparison to those achieved with C/N ratios of 5 and 20. Monod kinetic coefficients, half saturation concentration(Ks), maximum rate of substrate utilization(k), yield coefficient,(Y)and endogenous decay coefficient(Kd)indicated potential application of R31 in large-scale SND process. This is the first report on concomitant carbon oxidation, nitrification, and denitrification in the genusChryseobacteriumand the associated kinetic coefficients.

Integration of nitrification and denitrification by combining anoxic and aerobic conditions in a membrane bioreactor

2010 ◽  
Vol 62 (11) ◽  
pp. 2590-2598 ◽  
Author(s):  
Jianfeng Li ◽  
Fenglin Yang ◽  
Dieudonné-Guy Ohandja ◽  
Fook-Sin Wong
Keyword(s):  
Activated Sludge ◽  
Nitrogen Removal ◽  
Membrane Bioreactor ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Biomass Concentration ◽  
Morphological Properties ◽  
Utilization Rate ◽  
Gradient Gel Electrophoresis ◽  
Microbial Characterization ◽  
Nitrification And Denitrification

A membrane bioreactor (MBR) was developed to achieve nitrogen removal by combining nitrification and denitrification conditions in one reactor. The activated sludge was alternated between aerobic and anoxic conditions using peristaltic pump. The biomass concentration and floc morphological properties were observed to be similar in anoxic and aerobic compartments. However, the homogeneous properties of the activated sludge did not lead to the failure of oxygen gradient formation in the reactor. Due to the position of the air diffuser, an anoxic compartment at the bottom and an aerobic compartment in the upper part of the reactor were formed after 40 days. The average total nitrogen (TN) removal efficiency was then observed to increase to 77%. The microbial characterization using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) analysis, as well as the specific nitrogen utilization rate measurements, indicated that the nitrogen removal in the reactor occurred via nitrification and denitrification processes.

scholarly journals Nitrogen Removal Characteristics of Pseudomonas putida Y-9 Capable of Heterotrophic Nitrification and Aerobic Denitrification at Low Temperature

2017 ◽  
Vol 2017 ◽  
pp. 1-7 ◽  
Author(s):  
Yi Xu ◽  
Tengxia He ◽  
Zhenlun Li ◽  
Qing Ye ◽  
Yanli Chen ◽  
...  
Keyword(s):  
Low Temperature ◽  
Pseudomonas Putida ◽  
Nitrogen Removal ◽  
Removal Rate ◽  
Heterotrophic Nitrification ◽  
Oxidation Products ◽  
Aerobic Denitrification ◽  
Nitrification Rate ◽  
High Concentration ◽  
Nitrate And Nitrite

The cold-adapted bacterium Pseudomonas putida Y-9 was investigated and exhibited excellent capability for nitrogen removal at 15°C. The strain capable of heterotrophic nitrification and aerobic denitrification could efficiently remove ammonium, nitrate, and nitrite at an average removal rate of 2.85 mg, 1.60 mg, and 1.83 mg NL−1 h−1, respectively. Strain Y-9 performed nitrification in preference to denitrification when ammonium and nitrate or ammonium and nitrite coexisted in the solution. Meantime, the presence of nitrate had no effect on the ammonium removal rate of strain Y-9, and yet the presence of high concentration of nitrite would inhibit the cell growth and decrease the nitrification rate. The experimental results indicate that P. putida Y-9 has potential application for the treatment of wastewater containing high concentrations of ammonium along with its oxidation products at low temperature.

scholarly journals Effect of Cobalt, Cadmium and Manganese on Nitrogen Removal Capacity of Arthrobacter arilaitensis Y-10

Water ◽  
2020 ◽  
Vol 12 (6) ◽  
pp. 1701
Author(s):  
Tengxia He ◽  
Deti Xie ◽  
Jiupai Ni ◽  
Zhu Li ◽  
Zhenlun Li
Keyword(s):  
Nitrogen Removal ◽  
Inorganic Nitrogen ◽  
Heterotrophic Nitrification ◽  
Aerobic Denitrification ◽  
Inhibitory Influence ◽  
Inhibitory Effects ◽  
Removal Capacity ◽  
Nitrite Removal ◽  
Denitrification Activity ◽  
Nitrate And Nitrite

The aim of this study was to investigate the possibility of a simultaneous nitrification–denitrification hypothermic bacterium for applying in Cd(II), Co(II), and Mn(II)-contaminated wastewater. The influence of Cd(II), Co(II), and Mn(II) on the inorganic nitrogen removal capacity of the hypothermia bacterium Arthrobacter arilaitensis Y-10 was determined. The experimental results demonstrated that low concentration of Cd(II) (2.5 mg/L) exhibited no significant impact on bioremediation of ammonium. The nitrate and nitrite removal activities of strain Y-10 were enhanced by 0.1 and 0.25 mg/L of Cd(II), but hindered by more than 0.25 and 0.5 mg/L of Cd(II), respectively. However, the cell growth and denitrification activity ceased immediately once Co(II) was supplemented. In terms of Mn(II), no conspicuous inhibitory impact on ammonium bioremediation was observed even if Mn(II) concentration reached as high as 30 mg/L. The bioremediation of nitrates and nitrites was significantly improved by 0.5 mg/L of Mn(II), and then dropped sharply along with the increase of Mn(II). The order of the degree of inhibitory influence of the three heavy metal ions on the nitrogen bioremediation ability of strain Y-10 was Co(II) > Cd(II) > Mn(II). All the results highlighted that the heterotrophic nitrification was less sensitive to the inhibitory effects of Cd(II), Co(II), and Mn(II) relative to aerobic denitrification.

Energetics of enhanced biological phosphorus and nitrogen removal processes

1998 ◽  
Vol 38 (1) ◽  
pp. 177-184 ◽  
Author(s):  
Zlatko Tonkovic
Keyword(s):  
Redox Potential ◽  
Nitrogen Removal ◽  
Enzyme Induction ◽  
Phosphorus Release ◽  
Aerobic Denitrification ◽  
Strictly Aerobic ◽  
Removal Processes ◽  
Nitrification And Denitrification ◽  
Biological Phosphorus

Recent advances by researchers with in vivo NMR spectroscopy using labelled isotopes of carbon and phosphorus have elucidated some of the biochemical pathways involved in enhanced biological phosphorus and nitrogen removal processes. This has permitted a greater understanding of carbon and phosphorus cycling within cellular processes. All microbiological processes associated with energy transformations are dependent on enzyme induction. The enhanced phosphorus removal apparatus in certain organisms is dependent on the environmental conditions in order to induce the necessary enzymes. Enzyme expression has been linked to redox potential, which itself is a function of the culture medium and the bioenergetic condition of the cells. Redox balance is coupled to several pathways in the metabolic network of microorganisms. Changes to the redox potential result in shifts in the metabolic pathway utilisation. In the past, nitrification and denitrification have been thought to be strictly aerobic and anoxic processes respectively. Mounting evidence has demonstrated that this classical view of nitrification and denitrification is no longer valid and that other processes can occur such as simultaneous nitrification and denitrification and aerobic denitrification. Enzyme induction plays a very important role in these processes. Attempts have been made in this paper to elucidate the energetics of anaerobic phosphorus release based on cellular composition and various other plant operating parameters. Energetics of the denitrification process including anoxic dephosphatation, aerobic denitrification, with potential reduction in aerobic treatment requirements, are also explored.

scholarly journals Enhanced Nitrogen Removal of Wastewater at Low Temperature by Iterative Screening of Cold-Tolerant Denitrifying Bacteria

2021 ◽  
Author(s):  
Jin Qu ◽  
Zhanwang zheng ◽  
Ruojin Zhao ◽  
Yinyan Chen ◽  
Yiyi Li ◽  
...  
Keyword(s):  
Low Temperature ◽  
Nitrogen Removal ◽  
Domestic Wastewater ◽  
Specific Pcr ◽  
Moraxella Osloensis ◽  
Cold Tolerant ◽  
Nitrite Removal ◽  
Balance Analysis ◽  
Nitrification And Denitrification ◽  
Nitrate And Nitrite

Abstract The biological denitrification for wastewater treatment in winter is often seriously compromised due to the effects of low-temperature (<13 °C) on metabolic activity of microorganism. In this study, an excellent cold-tolerant denitrifying bacterium, Moraxella osloensis LT-01 was isolated by iterative domestication. The strain LT-01 retained about 60% maximal growth activity at 10 °C. Under initial concentrations of 100 mg/L, average ammonium, nitrate and nitrite removal efficiencies for domestic wastewater (C/N 4:1) at 10 °C were 70.35%, 65.39% and 61.74% in 24 h, respectively. Nitrogen balance analysis showed that about 46% of TN was directed toward in the dissimilation form of gas, and 16% of TN was assimilated for cell growth. Key genes hydroxylamine reductase gene (HAO) and nitrite reductase (NirS) involved in nitrification and denitrification processes were identified by gene-specific PCR, indicating that strain LT-01 perform nitrogen removal efficiently via unique simultaneous nitrification and denitrification. These results suggest the bacterium LT-01 has great potential as an effective performer for treating domestic wastewater in winter.

Enhancing nitrogen removal efficiency in a dyestuff wastewater treatment plant with the IFFAS process: the pilot-scale and full-scale studies

2017 ◽  
Vol 77 (1) ◽  
pp. 70-78 ◽  
Author(s):  
Yanjun Mao ◽  
Xie Quan ◽  
Huimin Zhao ◽  
Yaobin Zhang ◽  
Shuo Chen ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Removal Efficiency ◽  
Nitrogen Removal ◽  
Treatment Plant ◽  
Oxygen Demand ◽  
Pilot Scale ◽  
Full Scale ◽  
Nitrification And Denitrification ◽  
Dyestuff Wastewater

Abstract The activated sludge (AS) process is widely applied in dyestuff wastewater treatment plants (WWTPs); however, the nitrogen removal efficiency is relatively low and the effluent does not meet the indirect discharge standards before being discharged into the industrial park's WWTP. Hence it is necessary to upgrade the WWTP with more advanced technologies. Moving bed biofilm processes with suspended carriers in an aerobic tank are promising methods due to enhanced nitrification and denitrification. Herein, a pilot-scale integrated free-floating biofilm and activated sludge (IFFAS) process was employed to investigate the feasibility of enhancing nitrogen removal efficiency at different hydraulic retention times (HRTs). The results showed that the effluent chemical oxygen demand (COD), ammonium nitrate (NH4+-N) and total nitrogen (TN) concentrations of the IFFAS process were significantly lower than those of the AS process, and could meet the indirect discharge standards. PCR-DGGE and FISH results indicated that more nitrifiers and denitrifiers co-existed in the IFFAS system, promoting simultaneous nitrification and denitrification. Based on the pilot results, the IFFAS process was used to upgrade the full-scale AS process, and the effluent COD, NH4+-N and TN of the IFFAS process were 91–291 mg/L, 10.6–28.7 mg/L and 18.9–48.6 mg/L, stably meeting the indirect discharge standards and demonstrating the advantages of IFFAS in dyestuff wastewater treatment.

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