scholarly journals Effect of Nonviral Plasmid Delivered Basic Fibroblast Growth Factor and Low Intensity Pulsed Ultrasound on Mandibular Condylar Growth: A Preliminary Study

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Harmanpreet Kaur ◽  
Hasan Uludağ ◽  
Tarek El-Bialy
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Volume Fraction ◽  
Bone Volume ◽  
Fibroblast Growth ◽  
Pulsed Ultrasound ◽  
Bone Volume Fraction ◽  
Mandibular Growth ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity

Objective. Basic fibroblast growth factor (bFGF) is an important regulator of tissue growth. Previous studies have shown that low intensity pulsed ultrasound (LIPUS) stimulates bone growth. The objective of this study was to evaluate the possible synergetic effect of LIPUS and local injection of nonviral bFGF plasmid DNA (pDNA) on mandibular growth in rats.Design. Groups were control, blank pDNA, bFGF pDNA, LIPUS, and bFGF pDNA + LIPUS. Treatments were performed for 28 days. Significant increase was observed in mandibular height and condylar length in LIPUS groups. MicroCT analysis showed significant increase in bone volume fraction in bFGF pDNA + LIPUS group. Histomorphometric analysis showed increased cell count and condylar proliferative and hypertrophic layers widths in bFGF pDNA group.Results. Current study showed increased mandibular condylar growth in either bFGF pDNA or LIPUS groups compared to the combined group that showed only increased bone volume fraction.Conclusion. It appears that there is an additive effect of bFGF + LIPUS on the mandibular growth.

Transforming growth factor-β1 mediates the effects of low-intensity pulsed ultrasound in chondrocytes

2005 ◽  
Vol 31 (12) ◽  
pp. 1713-1721 ◽  
Author(s):  
Shogo Mukai ◽  
Hiromu Ito ◽  
Yasuaki Nakagawa ◽  
Haruhiko Akiyama ◽  
Masatomo Miyamoto ◽  
...  
Keyword(s):  
Growth Factor ◽  
Transforming Growth Factor ◽  
Transforming Growth Factor Β1 ◽  
Pulsed Ultrasound ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity

Abstract P278: Fibroblast Growth Factor 21 Mediated Protection of Ischemic Myocardium

2011 ◽  
Vol 109 (suppl_1) ◽  
Author(s):  
Shu Q Liu ◽  
Brandon Tefft ◽  
Alexei Kharitonenkov ◽  
Yupeng Ren ◽  
Li-Qun Zhang ◽  
...  
Keyword(s):  
Growth Factor ◽  
Myocardial Ischemia ◽  
Fibroblast Growth Factor ◽  
Protein Level ◽  
Volume Fraction ◽  
Secretory Protein ◽  
Fibroblast Growth Factor 21 ◽  
Coronary Artery Ligation ◽  
Fibroblast Growth ◽  
Artery Ligation

Myocardial ischemia is a prevalent disorder causing heart failure. As cardiomyocyte death is the principal cause of cardiac functional deficits, a recognized treatment is to minimize cardiomyocyte death post myocardial ischemia. While extensive investigations have been conducted, few effective cardioprotective agents have been developed for clinical applications. The goal of this investigation is to test and establish a cardioprotective agent based on fibroblast growth factor 21 (FGF21), a secretory protein participating in innate cardioprotective responses. In myocardial ischemia induced by LAD coronary artery ligation in the mouse, hepatocytes upregulated FGF21 mRNA by 11 folds at 12 hrs, followed by an increase in the FGF21 protein level in hepatocytes and the serum. Administration of recombinant FGF21 to mice immediately post MI (50 ng/gm, IV, twice/day for 3 days) resulted in a significant reduction in the fraction of myocardial infarction (MI) with reference to the LV wall volume below the LAD ligation (38+/−5 and 21+/−4%, n=7, vs. albumin administration 51+/−8 and 31+/−5%, n=6, at day 1 and 10, respectively, p<0.05 for both times) in association with improved LV dp/dt. In FGF21 overexpressing mice, the volume fraction of MI (36+/−6%, n=6) was significantly lower than that in wildtype mice (48+/−6%, n=5, p<0.05) at day 1 post MI. Furthermore, FGF21 administration to normal mice induced phosphorylation of FGFR1, PI3K, Akt, and BAD in cardiomyocytes within 10 – 30 min. These molecules were also phosphorylated in cardiomyocytes within 1 day post MI. Injection of siRNA specific to FGFR1, PI3K, or Akt to the LV anterior wall at 6 locations about 2 mm apart (diffusion range of FITC-siRNA tested by fluorescence microscopy) 3 days prior to MI resulted in a reduction in the protein level of FGFR1, PI3K, or Akt by 65+/−9, 71+/−11, or 68+/−12%, respectively (n=3), within the region of siRNA injection at 1 day post MI in association with a reduction in BAD phosphorylation and an increase in the fraction of MI by 9+/−3, 8+/−2, or 11+/−3%, respectively (n=3). These observations suggested that FGF21 contributed to myocardial protection possibly via the FGFR1-PI3K-Akt-BAD signaling mechanisms and recombinant FGF21 may be potentially used as a cardioprotective agent.

Effect of Low-Intensity Pulsed Ultrasound After Autologous Adipose-Derived Stromal Cell Transplantation for Bone-Tendon Healing in a Rabbit Model

2019 ◽  
Vol 47 (4) ◽  
pp. 942-953 ◽  
Author(s):  
Can Chen ◽  
Tao Zhang ◽  
Fei Liu ◽  
Jin Qu ◽  
Yang Chen ◽  
...  
Keyword(s):  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Volume Fraction ◽  
Treatment Time ◽  
Rabbit Model ◽  
The Other ◽  
Pulsed Ultrasound ◽  
Trabecular Thickness ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity

Background: Low-intensity pulsed ultrasound (LIPUS), as a safe biophysiotherapy, can enhance bone-tendon (B-T) healing in vivo and induce osteogenic or chondrogenic differentiation of mesenchymal stromal cells in vitro. This study aimed to determine whether LIPUS can improve the efficacy of transplanted mesenchymal stromal cells on B-T healing. Hypothesis: LIPUS can induce lineage-specific differentiation of transplanted adipose-derived stromal cells (ASCs) at the B-T healing site, thus resulting in superior healing quality when compared with LIPUS or ASCs alone. Study Design: Controlled laboratory study. Methods: A total of 112 mature rabbits with partial patellectomy in the hindlimb were randomly assigned into mock sonication without ASCs (control), ultrasonication without ASCs (LIPUS), mock sonication with ASCs (ASCs), and ultrasonication with ASCs (LIPUS + ASCs). The treatment time of the mock sonication or ultrasonication was 20 minutes per day. Autologous ASCs were transplanted to the healing site by fibrin glue during the operation, and LIPUS was delivered daily starting at postoperative day 3 until euthanasia. The patella–patellar tendon junctions were postoperatively harvested at 8 and 16 weeks for radiological, histological, and mechanical evaluations. Additionally, 9 animals were used for ASC tracking with mCherry protein. Results: Radiologically, there was more new bone formation and remodeling in the LIPUS + ASCs group as compared with the other groups. Synchrotron radiation micro–computed tomography showed that the LIPUS + ASCs group significantly increased bone volume fraction, trabecular thickness, and trabecular number at the healing site as compared with the other groups at postoperative 8 weeks ( P < .05 for all). Histologically, immunohistochemical staining confirmed that the transplanted mCherry-ASCs can differentiate into osteoblasts and fibrochondrocytic-like cells. Meanwhile, as compared with the other groups, the LIPUS + ASCs group showed more formation and maturity of the fibrocartilage layer and new bone at postoperative weeks 8 and 16 ( P < .05 for all). Biomechanically, the LIPUS + ASCs group showed significantly higher failure load and stiffness versus the other groups at postoperative weeks 8 and 16 ( P < .05 for all). Conclusion: Autologous ASC transplantation stimulated with LIPUS can result in superior B-T healing quality when compared with LIPUS or ASCs alone. Clinical Relevance: This study demonstrates the effectiveness of using ASC transplantation stimulated with LIPUS for B-T healing and provides a foundation for future clinical studies.

scholarly journals Low-Intensity Pulsed Ultrasound Stimulation of Condylar Growth in Rats

2009 ◽  
Vol 79 (5) ◽  
pp. 964-970 ◽  
Author(s):  
Rodrigo Oyonarte ◽  
Mariana Zárate ◽  
Francisco Rodriguez
Keyword(s):  
Layer Thickness ◽  
Mandibular Condyle ◽  
Sprague Dawley ◽  
Pulsed Ultrasound ◽  
Condylar Cartilage ◽  
Mandibular Growth ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity ◽  
Bone Level ◽  
And Control

Abstract Objective: To test the hypothesis that low-intensity pulsed ultrasound (LIPUS) stimulation does not histologically affect the growth of mandibular condylar cartilage. Materials and Methods: Thirty-five 20-day-old Sprague-Dawley rats were assigned to experimental and control groups. Experimental rats were stimulated with LIPUS in the temporomandibular joint (TMJ) region unilaterally, for 10 or 20 minutes for 20 days. After euthanasia, histological specimens were analyzed qualitatively and histomorphometrically at the anterior and posterior aspects of the mandibular condyle, including the condylar cartilage and the area and perimeter of subchondral bony trabeculae. Results: LIPUS stimulation may alter the histological arrangement of the condylar bone and cartilage, showing qualitative differences on specimens treated for 10 or 20 minutes daily compared with controls. Cartilaginous layer thickness was not affected by LIPUS stimulation to a significant level, but was modified at the relative layer thickness within the cartilage at the anterior aspect of the condyle (P &lt; .05). At the subchondral bone level, 20-minute stimulation significantly increases trabecular perimeter (P = .01). Conclusions: LIPUS application may affect mandibular growth pattern in rats acting at the cartilage and bone level. The effect of LIPUS on the growing condyle is expressed through a variation in trabecular shape and perimeter. A greater response is achieved when stimulated for 20 minutes instead of 10 minutes daily.

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