scholarly journals Generation of the Bovine Viral Diarrhea Virus E0 Protein in TransgenicAstragalusand Its Immunogenicity in Sika Deer

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Yugang Gao ◽  
Xueliang Zhao ◽  
Pu Zang ◽  
Qun Liu ◽  
Gongqing Wei ◽  
...  
Keyword(s):  
Transgenic Plant ◽  
Bovine Viral Diarrhea Virus ◽  
Rna Virus ◽  
Recombinant Protein Expression ◽  
Effective Vaccine ◽  
Bovine Viral Diarrhea ◽  
Diarrhea Virus ◽  
Viral Diarrhea ◽  
Viral Diarrhea Virus ◽  
Plant Vaccine

The bovine viral diarrhea virus (BVDV), a single-stranded RNA virus, can cause fatal diarrhea syndrome, respiratory problems, and reproductive disorders in herds. Over the past few years, it has become clear that the BVDV infection rates are increasing and it is likely that an effective vaccine for BVDV will be needed. In this study, transgenicAstragaluswas used as an alternative productive platform for the expression of glycoprotein E0. The immunogenicity of glycoprotein E0 expressed in transgenicAstragaluswas detected in deer. The presence of pBI121-E0 was confirmed by polymerase chain reaction (PCR), transcription was verified by reverse transcription- (RT-) PCR, and recombinant protein expression was confirmed by ELISA and Western blot analyses. Deer that were immunized subcutaneously with the transgenic plant vaccine developed specific humoral and cell-mediated immune responses against BVDV. This study provides a new method for a protein with weak immunogenicity to be used as part of a transgenic plant vaccine.

scholarly journals Transdominant Inhibition of Bovine Viral Diarrhea Virus Entry

2007 ◽  
Vol 82 (5) ◽  
pp. 2427-2436 ◽  
Author(s):  
Donna M. Tscherne ◽  
Matthew J. Evans ◽  
Margaret R. MacDonald ◽  
Charles M. Rice
Keyword(s):  
Bovine Viral Diarrhea Virus ◽  
Envelope Glycoprotein ◽  
Cell Clone ◽  
Rna Virus ◽  
Bovine Viral Diarrhea ◽  
Diarrhea Virus ◽  
Viral Diarrhea ◽  
Mdbk Cells ◽  
Positive Strand Rna ◽  
Viral Diarrhea Virus

ABSTRACT Bovine viral diarrhea virus (BVDV) is a positive-strand RNA virus and a member of the genus Pestivirus in the family Flaviviridae. To identify and characterize essential factors required for BVDV replication, a library expressing random fragments of the BVDV genome was screened for sequences that act as transdominant inhibitors of viral replication by conferring resistance to cytopathic BVDV-induced cell death. We isolated a BVDV-nonpermissive MDBK cell clone that harbored a 1.2-kb insertion spanning the carboxy terminus of the envelope glycoprotein 1 (E1), the envelope glycoprotein E2, and the amino terminus of p7. Confirming the resistance phenotype conferred by this library clone, naïve MDBK cells expressing this fragment were found to be 100- to 1,000-fold less permissive to both cytopathic and noncytopathic BVDV infection compared to parental MDBK cells, although these cells remained fully permissive to vesicular stomatitis virus. This restriction could be overcome by electroporation of BVDV RNA, indicating a block at one or more steps in viral entry prior to translation of the viral RNA. We determined that the E2 ectodomain was responsible for the inhibition to BVDV entry and that this block occurred downstream from BVDV interaction with the cellular receptor CD46 and virus binding, suggesting interference with a yet-unidentified BVDV entry factor.

Immunohistochemical Evidence for the Localization of Bovine Viral Diarrhea Virus, a Single-stranded RNA Virus, in Ovarian Oocytes in the Cow

1998 ◽  
Vol 35 (4) ◽  
pp. 253-259 ◽  
Author(s):  
M. D. Fray ◽  
H. Prentice ◽  
M. C. Clarke ◽  
B. Charleston
Keyword(s):  
Bovine Viral Diarrhea Virus ◽  
Rna Virus ◽  
Nonstructural Protein ◽  
Ovarian Tissue ◽  
Bovine Viral Diarrhea ◽  
Developmental Potential ◽  
Persistently Infected ◽  
Diarrhea Virus ◽  
Viral Diarrhea ◽  
Viral Diarrhea Virus

Bovine viral diarrhea virus (BVDV) is a single-stranded RNA virus responsible for enteric disease and reproductive failure in cattle. The virus can pass vertically from cow to fetus, causing abortion, birth of malformed calves, and calves born with persistent and life-long infections. In this study, we investigated the tropism of BVDV in ovarian tissue from persistently infected animals. Three heifers persistently infected with BVDV were euthanatized and their ovaries were recovered. A specimen of each ovary was taken ( n = 6) for virus isolation, and the remaining ovarian tissue was stored at −70 C. Cryosections (6 μm) cut from each ovary were analyzed for the presence of BVDV antigens by indirect immunofluorescence. The immunofluorescent analysis employed two monoclonal antibodies, WB103 and WB162, previously raised against the nonstructural protein NS3 and the envelop glycoprotein E2, respectively. High titers (6.97 ± 0.17 log10 tissue culture infective dose50/ml) of BVDV were recovered from 6/6 ovarian samples; NS3 and E2 were widely distributed within the ovarian stroma, the cumulus cell population, and the oocytes maturing in primordial, primary, and secondary follicles. Overall, 362/1,939 (18.7%) of the oocytes contained BVDV antigens, and there was no significant ( P > 0.05) difference in the proportion of BVDV-infected oocytes recorded within the primordial (227/1,247, 18.2%), primary (122/630, 19.4%), and secondary (13/62, 21.0%) follicle populations. Although the developmental potential of the infected oocytes could not be established in the present study, we conclude that bovine oocyte and the cumulus cells are susceptible to BVDV infection.

scholarly journals Traces of history conserved over 600 years in the geographic distribution of genetic variants of an RNA virus: Bovine viral diarrhea virus in Switzerland

PLoS ONE ◽  
2018 ◽  
Vol 13 (12) ◽  
pp. e0207604 ◽  
Author(s):  
Hanspeter Stalder ◽  
Claudia Bachofen ◽  
Matthias Schweizer ◽  
Reto Zanoni ◽  
Dominik Sauerländer ◽  
...  
Keyword(s):  
Geographic Distribution ◽  
Bovine Viral Diarrhea Virus ◽  
Genetic Variants ◽  
Rna Virus ◽  
Bovine Viral Diarrhea ◽  
Diarrhea Virus ◽  
Viral Diarrhea ◽  
Viral Diarrhea Virus

Prevention and elimination of bovine viral diarrhea virus infections in fetal fibroblast cells

2004 ◽  
Vol 64 (2) ◽  
pp. 113-118 ◽  
Author(s):  
M GIVENS ◽  
D STRINGFELLOW ◽  
C DYKSTRA ◽  
K RIDDELL ◽  
P GALIK ◽  
...  
Keyword(s):  
Bovine Viral Diarrhea Virus ◽  
Bovine Viral Diarrhea ◽  
Fibroblast Cells ◽  
Virus Infections ◽  
Fetal Fibroblast ◽  
Diarrhea Virus ◽  
Viral Diarrhea ◽  
Viral Diarrhea Virus
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