scholarly journals Stimuli-Sensitive Hydrogel Based on N-Isopropylacrylamide and Itaconic Acid for Entrapment and Controlled Release ofCandida rugosaLipase under Mild Conditions

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Nikola Milašinović ◽  
Zorica Knežević-Jugović ◽  
Nedeljko Milosavljević ◽  
Marija Lučić Škorić ◽  
Jovanka Filipović ◽  
...  
Keyword(s):  
Gastrointestinal Tract ◽  
Itaconic Acid ◽  
Candida Rugosa ◽  
Entrapment Efficiency ◽  
Temperature Sensitive ◽  
Stimuli Responsive ◽  
Mild Conditions ◽  
The Matrix ◽  
Model Protein ◽  
Stimuli Sensitive

Stimuli responsive pH- and temperature-sensitive hydrogel drug delivery systems, as those based on N-isopropylacrylamide (NiPAAm) and itaconic acid (IA), have been attracting much of the attention of the scientific community nowadays, especially in the field of drug release. By adjusting comonomer composition, the matrix is enabled to protect the incorporated protein in the highly acidic environment of upper gastrointestinal tract and deliver it in the neutral or slightly basic region of the lower intestine. The protein/poly(NiPAAm-co-IA) hydrogels were synthetized by free radical crosslinking copolymerization and were characterized concerning their swelling capability, mechanical properties, and morphology. The pore structure and sizes up to 1.90 nm allowed good entrapment of lipase molecules. Model protein, lipase fromCandida rugosa,was entrapped within hydrogels upon mild conditions that provided its protection from harmful environmental influences. The efficiency of the lipase entrapment reached 96.7%, and was dependent on the initial concentration of lipase solution. The swelling of the obtained hydrogels in simulated pH and temperature of gastrointestinal tract, the lipase entrapment efficiency, and its release profiles from hydrogels were investigated as well.

Efficient immobilization of lipase from Candida rugosa by entrapment into poly(N-isopropylacrylamide-co-itaconic acid) hydrogels under mild conditions

2012 ◽  
Vol 69 (3) ◽  
pp. 347-361 ◽  
Author(s):  
Nikola Milašinović ◽  
Nedeljko Milosavljević ◽  
Jovanka Filipović ◽  
Zorica Knežević-Jugović ◽  
Melina Kalagasidis Krušić
Keyword(s):  
Itaconic Acid ◽  
Candida Rugosa ◽  
Mild Conditions ◽  
Lipase From Candida Rugosa

scholarly journals Smart hydrogels based on itaconic acid for biomedical application

2009 ◽  
Vol 63 (6) ◽  
pp. 603-610
Author(s):  
Simonida Tomic ◽  
Maja Micic ◽  
Bojana Krezovic ◽  
Sava Dobic ◽  
Edin Suljovrujic ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Itaconic Acid ◽  
Biomedical Applications ◽  
Biomedical Application ◽  
In Vitro Study ◽  
Mechanical Analysis ◽  
Wound Dressings ◽  
Temperature Sensitive ◽  
Stimuli Responsive

pH and temperature sensitive hydrogels, based on 2-hydroxyethyl methacrylate (HEMA) and itaconic acid (IA) copolymers, were prepared by gamma irradiation and characterized in order to examine their potential use in biomedical applications. The influence of comonomer ratio in these smart copolymers on their morphology, mechanical properties, biocompatibility and microbe penetration capability was investigated. The mechanical properties of copolymers were investigated using the dynamic mechanical analysis (DMA), while their morphology was examined by scanning electron microscopy (SEM). The morphology and mechanical properties of these hydrogels were found to be suitable for most requirements of biomedical applications. The in vitro study of P(HEMA/IA) biocompatibility showed no evidence of cell toxicity nor any considerable hemolytic activity. Furthermore, the microbe penetration test showed that neither Staphylococcus aureus nor Escherichia coli passed through the hydogel dressing; thus the P(HEMA/IA) dressing could be considered a good barrier against microbes. All results indicate that stimuli-responsive P(HEMA/IA) hydrogels have great potential for biomedical applications, especially for skin treatment and wound dressings.

Smart Polymers and their Applications: A Review

2017 ◽  
Vol 8 (03) ◽  
Author(s):  
Gore S. A. ◽  
Gholve S. B. ◽  
Savalsure S. M. ◽  
Ghodake K. B. ◽  
Bhusnure O. G. ◽  
...  
Keyword(s):  
Oil Recovery ◽  
Water Soluble ◽  
Solution Temperature ◽  
Smart Polymers ◽  
Stimuli Responsive ◽  
Responsive Materials ◽  
Water Soluble Polymers ◽  
Commercial Applications ◽  
Stimuli Sensitive ◽  
External Stimuli

Smart polymers are materials that respond to small external stimuli. These are also referred as stimuli responsive materials or intelligent materials. Smart polymers that can exhibit stimuli-sensitive properties are becoming important in many commercial applications. These polymers can change shape, strength and pore size based on external factors such as temperature, pH and stress. The stimuli include salt, UV irradiation, temperature, pH, magnetic or electric field, ionic factors etc. Smart polymers are very promising applicants in drug delivery, tissue engineering, cell culture, gene carriers, textile engineering, oil recovery, radioactive wastage and protein purification. The study is focused on the entire features of smart polymers and their most recent and relevant applications. Water soluble polymers with tunable lower critical solution temperature (LCST) are of increasing interest for biological applications such as cell patterning, smart drug release, DNA sequencing etc.

Multi-Stimuli-Responsive Mesoporous Membranes and Effect of Molecular Architecture on Thermo- and pH-Responsive Behavior of the grafted Block Copolymer brushes

2019 ◽  
Author(s):  
Yanchun Tang ◽  
Kohzo Ito ◽  
Hideaki Yokoyama
Keyword(s):  
Polymer Brushes ◽  
Diblock Copolymers ◽  
Neutron Reflectivity ◽  
Molecular Architecture ◽  
Stimuli Responsive ◽  
Responsive Polymer ◽  
The Matrix ◽  
Stable Part ◽  
Copolymer Brushes ◽  
Mesoporous Membranes

In this study, we prepared ultrafiltration membranes with a decoupled responses of filtration property to temperature and pH. The membrane preparation method was developed based on our previous work. We utilized methanol-supercritical carbon dioxide (methanol-scCO<sub>2</sub>) selective swelling method to introduce nanopores to block copolymers containing poly(diethylene glycol) methyl ether methacrylate (PMEO<sub>2</sub>MA), poly(N,N-dimethylaminoethyl methacrylate) (PDMAEMA) and polystyrene (PS) blocks. Formation of the mesoporous barrier layer with PS being the mechanically stable part of the matrix was driven by selective swelling of the PMEO<sub>2</sub>MA-b-PDMAEMA domains. Due to the selective swelling of PMEO<sub>2</sub>MA or PDMAEMA domains to introduce pores, the interior of the pores are covered with PMEO<sub>2</sub>MA or PDMAEMA blocks after pore formation. The PMEO<sub>2</sub>MA-b-PDMAEMA polymer brushes are naturally attached on the pore walls and worked as functional gates. PMEO<sub>2</sub>MA is a non-toxic, neutral thermo-responsive polymer with LCST at 26 ᴼC. PDMAEMA is a typical weak polyelectrolyte with pK<sub>a</sub> value at 7.0-7.5 and also a thermo-responsive polymer revealed a LCST of 20-80 °C in aqueous solution. Therefore, these membranes were expected to have multi dimensions as function of the combination of temperature and pH. Moreover, to understand the detail of the temperature and pH depended conformation transitions of PMEO<sub>2</sub>MA-b-PDMAEMA brushes, those diblock copolymers were end-tethered on flat substrates and analyzed via neutron reflectivity (NR).

scholarly journals Characterization of Mmp37p, aSaccharomyces cerevisiaeMitochondrial Matrix Protein with a Role in Mitochondrial Protein Import

2006 ◽  
Vol 17 (9) ◽  
pp. 4051-4062 ◽  
Author(s):  
Michelle R. Gallas ◽  
Mary K. Dienhart ◽  
Rosemary A. Stuart ◽  
Roy M. Long
Keyword(s):  
Matrix Protein ◽  
Protein Import ◽  
Mitochondrial Protein ◽  
Temperature Sensitive ◽  
Inner Membrane ◽  
Mitochondrial Inner Membrane ◽  
Close Proximity ◽  
The Matrix ◽  
Targeting Signal

Many mitochondrial proteins are encoded by nuclear genes and after translation in the cytoplasm are imported via translocases in the outer and inner membranes, the TOM and TIM complexes, respectively. Here, we report the characterization of the mitochondrial protein, Mmp37p (YGR046w) and demonstrate its involvement in the process of protein import into mitochondria. Haploid cells deleted of MMP37 are viable but display a temperature-sensitive growth phenotype and are inviable in the absence of mitochondrial DNA. Mmp37p is located in the mitochondrial matrix where it is peripherally associated with the inner membrane. We show that Mmp37p has a role in the translocation of proteins across the mitochondrial inner membrane via the TIM23-PAM complex and further demonstrate that substrates containing a tightly folded domain in close proximity to their mitochondrial targeting sequences display a particular dependency on Mmp37p for mitochondrial import. Prior unfolding of the preprotein, or extension of the region between the targeting signal and the tightly folded domain, relieves their dependency for Mmp37p. Furthermore, evidence is presented to show that Mmp37 may affect the assembly state of the TIM23 complex. On the basis of these findings, we hypothesize that the presence of Mmp37p enhances the early stages of the TIM23 matrix import pathway to ensure engagement of incoming preproteins with the mtHsp70p/PAM complex, a step that is necessary to drive the unfolding and complete translocation of the preprotein into the matrix.

Self-assembly of Stimuli Responsive Coiled-coil Fibrous Hydrogels

Soft Matter ◽  
2021 ◽  
Author(s):  
Michael Meleties ◽  
Priya Katyal ◽  
Bonnie Lin ◽  
Dustin Britton ◽  
Jin Kim Montclare
Keyword(s):  
Drug Delivery ◽  
Tissue Engineering ◽  
Self Assembly ◽  
Coiled Coil ◽  
Stimuli Responsive ◽  
Tunable Properties ◽  
Stimuli Sensitive ◽  
Biomedical Fields

Owing to their tunable properties, hydrogels comprised of stimuli sensitive polymers are one of the most appealing scaffolds with applications in tissue engineering, drug delivery and other biomedical fields. We...

scholarly journals Conformation Study of Dual Stimuli-Responsive Core-Shell Diblock Polymer Brushes

Polymers ◽  
2018 ◽  
Vol 10 (10) ◽  
pp. 1084
Author(s):  
Kaimin Chen ◽  
Lan Cao ◽  
Ying Zhang ◽  
Kai Li ◽  
Xue Qin ◽  
...  
Keyword(s):  
Polymer Brushes ◽  
Core Shell ◽  
Stimuli Responsive ◽  
Research Topics ◽  
Design And Synthesis ◽  
Mild Conditions ◽  
The Core ◽  
Responsive Polymer ◽  
Stimuli Responsive Polymer ◽  
Shed Light

Stimuli-responsive nanoparticles are among the most popular research topics. In this study, two types of core-shell (polystyrene with a photoiniferter (PSV) as the core and diblock as the shell) polymer brushes (PSV@PNIPA-b-PAA and PSV@PAA-b-PNIPA) were designed and prepared using surface-initiated photoiniferter-mediated polymerization (SI-PIMP). Moreover, their pH- and temperature-stimuli responses were explored by dynamic light scattering (DLS) and turbidimeter under various conditions. The results showed that the conformational change was determined on the basis of the competition among electrostatic repulsion, hydrophobic interaction, hydrogen bonding, and steric hindrance, which was also confirmed by protein adsorption experiments. These results are not only helpful for the design and synthesis of stimuli-responsive polymer brushes but also shed light on controlled protein immobilization under mild conditions.

Stimuli-responsive cancer therapy based on nanoparticles

2014 ◽  
Vol 50 (79) ◽  
pp. 11614-11630 ◽  
Author(s):  
Jing Yu ◽  
Xin Chu ◽  
Yanglong Hou
Keyword(s):  
Magnetic Field ◽  
Cancer Therapy ◽  
Stimuli Responsive ◽  
Ph Reduction ◽  
Stimuli Sensitive

Nanoparticle (NP)-based stimuli-sensitive cancer therapy, including pH-, reduction-sensitive NPs and light-, magnetic field-responsive NPs are reviewed.

Programmable Skins based on Core-Shell Microsphere/Nanotube/Polymer Composites

2015 ◽  
Vol 1800 ◽  
Author(s):  
Balaji Panchapakesan ◽  
Cagdas Onal ◽  
James Loomis
Keyword(s):  
Polymer System ◽  
Liquid Hydrocarbon ◽  
Core Shell ◽  
Stimuli Responsive ◽  
Electrically Conductive ◽  
Dimensional Changes ◽  
Stimuli Responsive Polymers ◽  
Thermally Responsive ◽  
New Findings ◽  
The Matrix

ABSTRACTIn this paper, we describe unique thermally responsive polymer system based on nanotube-elastomers dispersed with core-shell expanding microspheres (phase-change material). Upon thermal or infrared stimuli, liquid hydrocarbon cores encapsulated within the microspheres vaporize, expanding the surrounding shells and stretching the matrix. Microsphere transformation resulted in visible dimensional changes associated with macroscopic volume increase (>500%), reduction in density (>80%), and increase in elastic modulus (>675%). Additionally, electrically conductive nanotubes allowed for expansion dependent electrical responses. We present our new findings on expansion dependent superhydrophobicity in these materials and present some outlook and comparison of our stimuli responsive polymers with other material systems for future origami based applications.

Development of a probiotic non-fermented blend beverage with juçara fruit: Effect of the matrix on probiotic viability and survival to the gastrointestinal tract

LWT ◽  
2020 ◽  
Vol 118 ◽  
pp. 108756 ◽  
Author(s):  
Ana Paula de Oliveira Ribeiro ◽  
Flávia dos Santos Gomes ◽  
Karina Maria Olbrich dos Santos ◽  
Virgínia Martins da Matta ◽  
Daniela de Grandi Castro Freitas de Sá ◽  
...  
Keyword(s):  
Gastrointestinal Tract ◽  
The Matrix
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