scholarly journals A Peripheral Ameloblastic Fibro-Odontoma in a 3-Year-Old Girl: Case Report, Immunohistochemical Analysis, and Literature Review

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Yi-Chun Lin ◽  
Hsiu-Ming Hsu ◽  
Chiang-Shin Liu ◽  
Kuo Yuan
Keyword(s):  
Histopathological Examination ◽  
Correct Diagnosis ◽  
Immunohistochemical Analysis ◽  
Clinical Appearance ◽  
Jaw Bones ◽  
Tissue Sections ◽  
Odontogenic Epithelium ◽  
Histological Characteristics ◽  
Children And Young Adults

Ameloblastic fibro-odontoma (AFO) predominantly occurs in the jaw bones of children and young adults. Extraosseous AFO is extremely rare. We describe a peripheral ameloblastic fibro-odontoma in the maxillary gingiva of a 3-year-old girl. The clinical appearance resembled fiery red reactive gingival lesions. The histopathological examination of the excised lesion showed small islands and cords of odontogenic epithelium with cellular myxoid stroma in the subepithelial tissue. The mass contained calcified material and an enamel-like deposit. Many small blood vessels appeared in the connective tissue surrounding the odontogenic epithelium. The immunohistochemical assays showed strong reactivity for amelogenin,β-catenin, CD44, and CD31 in the tissue sections. There was no recurrence after the 1-year follow-up. Because this lesion clinically resembles other nonneoplastic lesions and is very rare in gingiva, establishing a correct diagnosis is achieved only based on specific histological characteristics. Conservative excision of the tumor is the treatment of choice.

scholarly journals Diagnostic Utility of AMCAR and p63 Cocktail Antibody in the Benign and Malignant Lesions of Prostate

2020 ◽  
Vol 7 (11) ◽  
pp. A531-537
Author(s):  
Sujitha Chougani ◽  
Sunandalakshmi G V ◽  
Durga Kharidehal ◽  
Ravi Sankar V ◽  
Santhi Vissa
Keyword(s):  
Histopathological Examination ◽  
Immunohistochemical Analysis ◽  
Cost Effective ◽  
Basal Cells ◽  
Variable Intensity ◽  
Atypical Small Acinar Proliferation ◽  
Positive Immunostaining ◽  
Malignant Lesions ◽  
Made In

Background: Histopathological examination of prostatic specimen is gold standard for the diagnosis of prostate cancer. Current study evaluates the expression of AMACR and p63 in the prostate lesions using AMACR and p63 cocktail. Materials and Method: Total of 180 cases were collected and Haematoxylin and Eosin staining performed followed by immunohistochemical analysis using AMACR and p63 antibody. Result: Out of 180 cases, Benign Prostatic Hyperplasia is the most common lesion noted in about 120 cases. In this study, the predominant population was in the 6th to 7th decade of age. Most of the patients presented with difficulty in micturition. Immunohistochemistry revealed that p63 expression is positive in all normal basal cells, 118 cases (98.33%) were negative for AMACR and only 2 cases were showing focal and weak AMACR immunoreactivity. AMACR was positive in all the 6 HGPIN cases with variable intensity. Out of 5 LGPIN cases AMACR was positive in 3 cases with low intensity, remaining 2 cases shows AMACR negative. p63 is positive in all 11 PIN cases (LGPIN & HGPIN) showing discontinuous staining pattern. All 22 cases of Prostatic adenocarcinomas were negative for p63 and all cases expressed positive immunostaining with AMACR. A diagnosis of adenocarcinoma was made in 48% of atypical cases. Cases which were negative for both AMACR and p63 were diagnosed as Atypical Small Acinar Proliferation, for which further follow-up is required.   Conclusion: AMACR/p63 Cocktail antibody is very much useful as it saves time, tissue and is cost-effective.

scholarly journals Perianal rhabdomyomatous mesenchymal hamartoma

2017 ◽  
Vol 99 (6) ◽  
pp. e193-e195 ◽  
Author(s):  
K Sampat ◽  
E Cheesman ◽  
S Siminas
Keyword(s):  
Histopathological Examination ◽  
Neck Region ◽  
Male Infant ◽  
Mesenchymal Hamartoma ◽  
Soft Tissue Tumour ◽  
Anal Lesion ◽  
Tissue Tumour ◽  
The Face ◽  
Histological Characteristics

A rhabdomyomatous mesenchymal hamartoma (RMH) is a rare benign soft tissue tumour found in the face and neck region of children. A three-month-old male infant was referred to our unit with a polypoid anal lesion that had been present from birth. Histopathological examination of the excised sample showed haphazard arrangement of mature striated skeletal muscle in among nerves and blood vessels, and immunohistochemistry confirmed the lesion to be a RMH. There was no evidence of recurrence during the follow-up period. This case emphasises two points: the unique histological characteristics associated with RMH and how this allows distinction from other differential diagnoses, and the consideration of RMH as a diagnosis in perianal lesions.

scholarly journals Direct Evidence of Active SARS-CoV-2 Replication in the Intestine

2020 ◽  
Author(s):  
Qun Qian ◽  
Lifang Fan ◽  
Weicheng Liu ◽  
Jin Li ◽  
Junqiu Yue ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Direct Evidence ◽  
Immune Cell ◽  
Histopathological Examination ◽  
Rectal Adenocarcinoma ◽  
Rectal Mucosa ◽  
Immunohistochemical Analysis ◽  
Mucosal Damage ◽  
Pathological Changes

Abstract Background Currently, there is no direct evidence to prove the active replication of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in the intestinal tract and relevant pathological changes in the colon and rectum. We investigated the presence of virions and pathological changes in surgical rectal tissues of a patient with clinically confirmed coronavirus disease 2019 (COVID-19) with rectal adenocarcinoma. Methods The clinical data were collected during hospitalization and follow-up of this patient. Quantitative reverse transcriptase–polymerasechain reaction (RT-PCR) was performed on the rectal tissue specimens obtained from surgical resection, succus entericus and intestinal mucosa of ileostomy, and rectal mucosa during follow-up after recovery. Ultrathin sections of surgical samples were observed for SARS-CoV-2 virions using electron microscopy. Histopathological examination was performed using hematoxylin-eosin stain. Immunohistochemical analysis and immunofluorescence were carried out on rectal tissues to evaluate the distribution of SARS-CoV-2 antigen and immune cell infiltrations. Results The patient had fever and cough on day 3 postoperatively, was diagnosed with COVID-19 on day 7, and was discharged from the hospital on day 41. RNA of SARS-CoV-2 was detected in surgically resected rectal specimens but not in samples collected 37 days after discharge. Notably, coincident with rectal tissues of surgical specimens testing nucleic acid positive for SARS-CoV-2, typical coronavirus virions in rectal tissue were observed under electron microscopy. Moreover, abundant lymphocytes and macrophages (some were SARS-CoV-2 positive) infiltrating the lamina propria were found with no significant mucosal damage. Conclusions We first report the direct evidence of active SARS-CoV-2 replication in a patient’s rectum during the incubation period, which might explain SARS-CoV-2 fecal–oral transmission.

Massive juvenile ossifying fibroma arising from the middle turbinate

2020 ◽  
Vol 13 (4) ◽  
pp. e234432
Author(s):  
Valentinos Sofokleous ◽  
Konstantina Chrysouli ◽  
Efthymios Kyrodimos ◽  
Evangelos Giotakis
Keyword(s):  
Histopathological Examination ◽  
Therapeutic Option ◽  
Correct Diagnosis ◽  
Middle Turbinate ◽  
Ossifying Fibroma ◽  
Endoscopic Endonasal ◽  
Juvenile Ossifying Fibroma ◽  
History Of

A 19-year-old man presented with a long-standing history of nasal obstruction, which gradually became worse over the past 2 years. Nasal endoscopy revealed a sizeable rounded mass covered by a normal-looking mucosa. Imaging studies showed a mass arising from the left middle turbinate that extended throughout the expanse of the anterior skull base. The tumour was resected via an endoscopic endonasal approach. Histopathological examination revealed a psammomatoid juvenile ossifying fibroma. The patient remains free of recurrence after almost 3 years of follow-up. Only four cases of ossifying fibroma with middle turbinate localisation have been reported in the literature so far, with our case representing the fifth and most extensive case. Clinical, radiological and histological findings should all be considered for establishing the correct diagnosis. An endoscopic approach represents an excellent therapeutic option. Long-term clinical and radiological surveillance is required due to the risk of recurrence.

scholarly journals Topical rapamycin reduces markers of senescence and aging in human skin: an exploratory, prospective, randomized trial

GeroScience ◽  
2019 ◽  
Vol 41 (6) ◽  
pp. 861-869 ◽  
Author(s):  
Christina Lee Chung ◽  
Ibiyonu Lawrence ◽  
Melissa Hoffman ◽  
Dareen Elgindi ◽  
Kumar Nadhan ◽  
...  
Keyword(s):  
Human Skin ◽  
Immunohistochemical Analysis ◽  
Intrinsic Rate ◽  
Skin Aging ◽  
Clinical Appearance ◽  
Protein Levels ◽  
Age Related ◽  
Lost To Follow Up ◽  
Collagen Vii

AbstractAging is a major risk factor for the majority of human diseases, and the development of interventions to reduce the intrinsic rate of aging is expected to reduce the risk for age-related diseases including cardiovascular disease, cancer, and dementia. In the skin, aging manifests itself in photodamage and dermal atrophy, with underlying tissue reduction and impaired barrier function. To determine whether rapamycin, an FDA-approved drug targeting the mechanistic target of rapamycin (mTOR) complex, can reduce senescence and markers of aging in human skin, an exploratory, placebo-controlled, interventional trial was conducted in a clinical dermatology setting. Participants were greater than 40 years of age with evidence of age-related photoaging and dermal volume loss and no major morbidities. Thirty-six participants were enrolled in the study, and nineteen discontinued or were lost to follow-up. A significant (P = 0.008) reduction in p16INK4A protein levels and an increase in collagen VII protein levels (P = 0.0077) were observed among participants at the end of the study. Clinical improvement in skin appearance was noted in multiple participants, and immunohistochemical analysis revealed improvement in histological appearance of skin tissue. Topical rapamycin reduced the expression of the p16INK4A protein consistent with a reduction in cellular senescence. This change was accompanied by relative improvement in clinical appearance of the skin and histological markers of aging and by an increase in collagen VII, which is critical to the integrity of the basement membrane. These results indicate that rapamycin treatment is a potential anti-aging therapy with efficacy in humans.Trial registration ClinicalTrials.gov Identifier: NCT03103893.

scholarly journals Adenomatoid Odontogenic Tumour Associated with Dentigerous Cyst: Case Report with Literature Review

2021 ◽  
Author(s):  
Pradipta Ramgonda Patil ◽  
Hirkani Attarde ◽  
Priyanka Prakash Kamble ◽  
Sangeeta Patankar ◽  
Gokul Sridharan
Keyword(s):  
Histopathological Examination ◽  
Dentigerous Cyst ◽  
Odontogenic Tumour ◽  
Odontogenic Epithelium ◽  
Microscopic Evaluation ◽  
Patient Reported ◽  
Hybrid Variety ◽  
Brownish Black ◽  
Adenomatoid Odontogenic Tumour

Adenomatoid Odontogenic Tumour (AOT) is a relatively uncommon benign odontogenic tumour composed of odontogenic epithelium in a variety of histoarchitectural patterns. Conventional AOT is predominantly seen in females in second decade of life exhibiting predilection for anterior region of maxilla. Very few cases of AOT associated with dentigerous cyst have been reported till date. The present case is unique associated with an impacted lower first premolar in the front region of the mandible of an 18-year-old female patient. The patient reported to the institute with gradually increasing swelling. The orthopantomogram revealed a unilocular radiolucency with displaced premolar. Histopathological examination confirmed 4×2.5×2 cm lesion in dimension, brownish black in colour, firm in consistency with a smooth surface grossly while cut surface revealed a cystic lumen with an impacted premolar embedded into the lining, cystic wall being nodular. Microscopic evaluation revealed a well encapsulated lesion with a thin 2-3 cell layered non keratinised stratified epithelial lining in patterns such as nests, rosette like structures and duct like structures. Thus the diagnosis of AOT arising from dentigerous cyst was confirmed. Patient's six month follow-up was uneventful. Literature search of similar cases with the review of hypothesised aetiopathogenesis is discussed in brief. The available data can help researchers resolve the uncertainty whether the AOT derived from dentigerous cyst could represent a distinct hybrid variety.

scholarly journals CERATOCISTO ODONTOGÊNICO EM MAXILA: RELATO DE CASO CLÍNICO

2020 ◽  
Author(s):  
Leticia Gonçalves Ferreira
Keyword(s):  
Cystic Lesion ◽  
Histopathological Examination ◽  
Hospital Environment ◽  
Odontogenic Keratocyst ◽  
Radiographic Examination ◽  
Medical Procedure ◽  
Surgical Specimen ◽  
Odontogenic Epithelium ◽  
Odontogenic Origin

Resumo      O Ceratocisto Odontogênico (CO)é uma lesão intraóssea benigna de origem odontogênica, que surge a partir dos restos celulares da lâmina dental; apresenta predominância de acometimento em mandíbula, principalmente com envolvimento do corpo posterior e ramo de mandíbula. Os COs podem ser encontrados em pacientes desde a infância até a velhice, todavia, mais da metade dos casos são diagnosticados em pessoas entre 10 a 40 anos de idade, sendo sua prevalência em homens. Tal lesão cística exibe ao exame radiográfico uma área radio lúcida, com margens escleróticas frequentemente bem definidas. Histologicamente manifesta revestimento epitelial composto por uma camada uniforme de epitélio escamoso estratificado, geralmente com 6 ou 8 camadas de espessura, podem ser observados ainda pequenos cistos, cordões ou ilhas satélites de epitélio odontogênico na cápsula fibrosa, a qual é tipicamente delgada e friável. O objetivo do presente trabalho é relatar o caso clínico do paciente BSM,sexo masculino, feoderma, 22 anos de idade, ASA I, que compareceu ao ambulatório de Buco-Maxilo-Facial do Hospital Federal de Ipanema/RJapresentando uma lesão cística associada ao terceiro molar superior direito incluso. O tratamento consistiu na enucleação e curetagem da lesãoem ambiente hospitalar sob anestesia geral, sem presença de intercorrências. A peça cirúrgica foi encaminhada ao Laboratório de Biotecnologia Aplicada da Universidade Federal Fluminense (LABA-UFF) para exame anatomohistopatológico, sendo confirmado seu diagnóstico inicial de CO. O paciente recebeu alta no dia seguinte ao procedimento cirúrgico e segue em acompanhamento ambulatorial de 12 meses pela especialidade, sem presença clínica e imaginológica de recidiva da lesão. Palavras-chave: Ceratocisto Odontogênico; Enucleação; Maxila Abstract  The Odontogenic Keratocyst (CO) is a benign intraosseous lesion of odontogenic origin, which increases from the cellular remains of the dental membrane; it presents predominance of follow-up in the mandible, mainly with involvement of the posterior body and mandible branch. OCs can be found in patients from childhood to old age, however, more than half of the cases are diagnosed in people between 10 and 40 years of age, being its prevalence in men. Such a cystic lesion is detected in the radiographic examination of a radiolucent area, with sclerotic margins often quite reduced. Histologically, the epithelial lining composed of a uniform layer of stratified stratified epithelium, with 6 or 8 layers of thickness, can also be observed in small cysts, cords or satellite islands of odontogenic epithelium in the fibrous capsule, which is typically thin and friable. The objective of the present work is to relate the clinical case of BSM patient, male, feoderma, 22 years old, ASA I, who compared the cystic lesion associated with the included dental element to the buccomaxillofacial clinic of the Federal Hospital of Ipanema / RJ. Treatment consists of enucleation and curation of lesions in the hospital environment under general anesthesia, without the presence of complications. A surgical specimen was sent to the Laboratory of Applied Biotechnology by Universidade Federal Fluminense (LABA-UFF) for anatomo-histopathological examination, confirming its initial diagnosis of OC. The patient who was discharged the day after the medical procedure and is undergoing outpatient follow-up for 12 months, with no clinical and imaginative presence of injury recurrence. Keywords: Odontogenic Keratocyst; Enucleation; Maxilla

scholarly journals Adenocarcinoma of the esophagogastric junction: relationship between clinicopathological data and p53, cyclin D1 and Bcl-2 immunoexpressions

2009 ◽  
Vol 46 (4) ◽  
pp. 315-320 ◽  
Author(s):  
Dárcio Matenhauer Lehrbach ◽  
Ivan Cecconello ◽  
Ulysses Ribeiro Jr ◽  
Vera Luiza Capelozzi ◽  
Alexandre Muxfeldt Ab'Saber ◽  
...  
Keyword(s):  
Survival Rate ◽  
Cyclin D1 ◽  
Esophagogastric Junction ◽  
Immunohistochemical Analysis ◽  
Clinicopathological Characteristics ◽  
Tnm Staging ◽  
Tissue Sections ◽  
Esophagogastric Junction Adenocarcinoma ◽  
Patient’S Outcome

CONTEXT: Esophagogastric junction adenocarcinoma has an aggressive behavior, and TNM (UICC) staging is not always accurate enough to categorize patient's outcome. OBJECTIVES: To evaluated p53, cyclin D1 and Bcl-2 immunoexpressions in esophagogastric junction adenocarcinoma patients, without Barrett's esophagus, and to compared to clinicopathological characteristics and survival rate. METHODS: Tissue sections from 75 esophagogastric junction adenocarcinomas resected from 1991 to 2003 were analyzed by immunohistochemistry for p53, cyclin D1 and Bcl-2 using streptavidin-biotin-peroxidase method. The mean follow-up time was 60 months SD = 61.5 (varying from 4 to 273 months). RESULTS: Fifty (66.7%) of the tumors were intestinal type and 25 (33.3%) were diffuse. Vascular, lymph node and perineural infiltration were verified in 16%, 80% and 68% of the patients, respectively. The patients were distributed according to the TNM staging in IA in 4 (5.3%), IB in 10 (13.3%), II in 15 (20%), IIA in 15 (20%), IIIB in 15 (20%) and IV in 16 (21.3%). Immunohistochemical analysis was positive for p53, cyclin D1 and bcl-2 in 68%, 18.7% and 100%, respectively. There was no association between immunoexpression and vascular and/or perineural invasions, clinicopathological characteristics and patients' survival rate. CONCLUSION: In this selected population, there was no association between the immunomarkers, p53, cyclin D1 and bcl-2 and clinicopathological data and/or overall survival.

scholarly journals Giant gastric carcinosarcoma infiltrating the left hepatic lobe: a case report

2021 ◽  
pp. 214
Author(s):  
◽  
◽  
Keyword(s):  
Gastric Resection ◽  
Histopathological Examination ◽  
Correct Diagnosis ◽  
Immunohistochemical Analysis ◽  
Needle Aspiration ◽  
Left Hepatic Lobe ◽  
En Bloc ◽  
Hepatic Lobe ◽  
Biopsy Specimens ◽  
Resected Tissue

Gastric carcinosarcoma is a rare biphasic tumor that consists of both carcinomatous and sarcomatous components. Clinical presentation and neoplastic markers are not specific for the disease. Moreover, CT scan cannot distinguish among gastric carcinoma, sarcoma or carcinosarcoma, so it is essential to perform histopathological and immunohistochemical analysis on biopsy specimens or resected tissue to ensure a correct diagnosis. A 72-year-old woman was transferred to our Department from another hospital with a diagnosis of a bleeding tumor localized on the lesser gastric curve, infiltrating the left hepatic lobe. The mass was treated as a gastric malignant mesenchymal neoplasia (gastro-intestinal stromal tumor versus sarcoma) infiltrating the liver. Owing to the bleeding, no further invasive examinations, such as fine needle aspiration biopsy, were planned. Furthermore, no neoadjuvant chemotherapy could be administered before surgery due to persistent anaemia. The scheduled surgical procedure was an atypical gastric resection with “en bloc” hepatic resection. This operation included upper polar and lesser curve resection, with tubulization of the stomach along the greater curve, as well as left lateral hepatic sectionectomy. Histopathological examination and immunohistochemical staining confirmed the diagnosis of gastric carcinosarcoma. This is first report of gastric carcinosarcoma with local liver infiltration, treated with surgical resection.

Characteristics of Different Types of Childhood Violence and the Risk of Revictimization

2019 ◽  
Vol 25 (14) ◽  
pp. 1696-1716 ◽  
Author(s):  
Ida Frugaard Stroem ◽  
Helene Flood Aakvaag ◽  
Tore Wentzel-Larsen
Keyword(s):  
Childhood Abuse ◽  
Telephone Survey ◽  
Regression Analyses ◽  
Multiple Forms ◽  
Different Types ◽  
Children And Young Adults ◽  
Childhood Violence ◽  
Forms Of Violence ◽  
The Relationship

This study investigates the relationship between the characteristics of different types of childhood violence and adult victimization using two waves of data from a community telephone survey (T1) and a follow-up survey, including 505 cases and 506 controls, aged 17-35 years (T2). The logistic regression analyses showed that exposure to childhood abuse, regardless of type, was associated with adult victimization. Exposure to multiple types of abuse, victimization both in childhood and in young adulthood, and recency of abuse increased these odds. Our findings emphasize the importance of assessing multiple forms of violence when studying revictimization. Practitioners working with children and young adults should be attentive to the number of victimization types experienced and recent victimization to prevent further abuse.

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