scholarly journals Antioxidant/Prooxidant and Antibacterial/Probacterial Effects of a Grape Seed Extract in Complex with Lipoxygenase

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Veronica Sanda Chedea ◽  
Cornelia Braicu ◽  
Flore Chirilă ◽  
Henry Joseph Oduor Ogola ◽  
Rodica Ştefania Pelmuş ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Control Level ◽  
Grape Seed Extract ◽  
Grape Seed ◽  
Seed Extract ◽  
Grape Seeds ◽  
E Coli ◽  
Prooxidant Action ◽  
High Level ◽  
Short Time

In an attempt to determine the antioxidant/prooxidant, antibacterial/probacterial action of flavan-3-ols and procyanidins from grape seeds, pure catechin (CS), and an aqueous grape seed extract (PE), were applied in the absence and presence of pure lipoxygenase (LS) or in extract (LE) to leucocyte culture,Escherichia coli B41andBrevibacterium linens, and observed whether there was any effect on lipid peroxidation, cytotoxicity, or growth rate. Short time periods of coincubation of cells with the polyphenols, followed by the exposure to LS and LE, revealed a high level of lipid peroxidation and a prooxidative effect. Longer coincubation and addition of LS and LE resulted in the reversal of the prooxidant action either to antioxidant activity for CS + LS and PE + LS or to the control level for CS + LE and PE + LE. Lipid peroxidation was significantly reduced when cells were exposed to polyphenols over a longer period. Longer exposure ofE. colito CS or PE followed by addition of LS for 3 h resulted in bactericidal activity. Significant stimulatory effect on microbial growth was observed for PE + LS and PE + LE treatments inB. linens, illustrating the potential probacterial activity inB. linenscultures. Lipoxygenase-polyphenols complex formation was found to be responsible for the observed effects.

Grape Seeds Extract as Brain Food: A Review

2017 ◽  
Vol 9 (1) ◽  
Author(s):  
Souad El Gengaihi ◽  
Doha H. Abou Baker
Keyword(s):  
Antioxidant Activities ◽  
Complex Mixture ◽  
Grape Seed Extract ◽  
Grape Seed ◽  
Seed Extract ◽  
Amyloid Peptide ◽  
Grape Seeds ◽  
Potential Benefits ◽  
Reduced Risk

Interest in the biological role of bioactive compounds present in medicinal herbs has increased over the last years. Of particular interest are plants that have an anti-Alzheimer activities. Several plants can be useful for Alzheimer (AD) management. Such as these which have anti-inflammatory activity, acetylcholinesterase (AChE) inhibitory action, antiapoptotic, slow the aggregation of amyloid peptide and antioxidant activities. Grape seed extract (GSE) is a complex mixture of several compounds, mostly represented by polyphenols and flavonoids. Their consumption is safe and is recognized to exert several health benefits. GS flavonoids have been associated with the reduced risk of chronic diseases, we present some findings on the potential benefits of GSE for the treatment of AD.

scholarly journals Effects of Grape Seed Extract, Vitamin C, and Vitamin E on Ethanol- and Aspirin-Induced Ulcers

2011 ◽  
Vol 2011 ◽  
pp. 1-6 ◽  
Author(s):  
Vivian Molina Cuevas ◽  
Yazmín Ravelo Calzado ◽  
Yohani Pérez Guerra ◽  
Ambar Oyarzábal Yera ◽  
Sonia Jiménez Despaigne ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Gastric Ulcer ◽  
Vitamin E ◽  
Grape Seed Extract ◽  
Grape Seed ◽  
Positive Control ◽  
Negative Control ◽  
Seed Extract ◽  
Gastric Ulcers ◽  
Control Group

Effects of GSE and vitamins C and E on aspirin- and ethanol-induced gastric ulcer and associated increases of lipid peroxidation in rats were compared. Two experiments were conducted. Rats were randomized into eight groups: a negative control and seven groups that received aspirin or ethanol for ulcer induction: one positive control (vehicle) and six with VC, VE, or GSE (25 and 250 mg/kg). Ulcer indexes and gastric levels of malondialdehyde (MDA) were quantified. VC, VE, and GSE (25 and 250 mg/kg) decreased aspirin, and ethanol-induced ulcers and MDA values compared with positive control group. The magnitude of aspirin ulcer reduction was comparable for all treatments, and MDA decrease with GSE was higher than with VC and tended to be greater, albeit none significantly, than with VE. GSE was more effective than VC and VE for lowering the ethanol ulcers, while the decrease of MDA levels with GSE was greater than with VC, but comparable to that achieved with VE. GSE protected against ethanol-induced gastric ulcers more effectively than VC or VE, while its protection against aspirin ulcers was comparable for all treatments. GSE produced the greatest reductions of gastric MDA in both models.

scholarly journals A study on antibacterial effect of grape seed extracts in common clinical and drug resistant isolates

2016 ◽  
Vol 3 (3) ◽  
pp. 165
Author(s):  
Mohanakrishnan Kandasamy ◽  
Sowmya Nasimuddin ◽  
Jeevan Malayan ◽  
Nithyalakshmi J ◽  
Sumathi Gnanadesikan ◽  
...  
Keyword(s):  
Antibacterial Effect ◽  
Grape Seed Extract ◽  
Grape Seed ◽  
Seed Extract ◽  
Clinical Isolates ◽  
Drug Resistant ◽  
Grape Seeds ◽  
Mrsa Strains ◽  
Agar Well Diffusion Assay ◽  
Grape Seed Extracts

<p class="abstract"><strong>Background:</strong> Grape seeds are proposed to have antimicrobial activity, antioxidant effect and various other benefits to mankind. A study was done to assess the antibacterial effect of grape seed extract against common clinical isolates and drug resistant pathogenic strains.</p><p class="abstract"><strong>Methods:</strong> Grape seed extract prepared was investigated for its antibacterial effect against 65 bacterial isolates obtained from clinical specimens by agar well diffusion assay and the results were compared with routinely used antibiotics namely, Gentamicin for the common clinical isolates, Vancomycin for MRSA strains and Amikacin for ESBL organisms respectively.</p><p class="abstract"><strong>Results:</strong> Grape seed extract produced moderate zone of inhibition ranging between 11-15 mm among the 35 test common clinical isolates namely <em>Staphylococcus aureus, Escherichia coli, Klebsiella </em>sp<em> </em>and<em> Pseudomonas aeruginosa</em>.  <em>E.coli </em>showed the highest susceptibility with zone ranging from 12-14 mm with increasing concentration of the extract starting from2 mg/ml to the highest being 20 mg/ml. Among the 30 drug resistant pathogenic strains like MRSA and ESBL producing organisms, the grape seed extract was found to be effective against 3 out of the 10 of MRSA and 2 out of the 10 of ESBL-<em>E.coli</em> at the highest concentration of 20 mg/ml. However, ESBL producing <em>Klebsiella </em>species were found to be resistant even to the highest concentration of the extract.  </p><p><strong>Conclusions:</strong> The results provide evidence that the grape seed extract could be a potential antibacterial agent and this effect can further be made evident with improved methodologies.</p>

scholarly journals Effects of Polyphenols from Grape Seeds on Renal Lithiasis

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Felix Grases ◽  
Rafel M. Prieto ◽  
Rafel A. Fernandez-Cabot ◽  
Antonia Costa-Bauzá ◽  
Fernando Tur ◽  
...  
Keyword(s):  
Ethylene Glycol ◽  
Calcium Oxalate ◽  
Complex Disease ◽  
Urine Analysis ◽  
Calcium Oxalate Monohydrate ◽  
Grape Seed Extract ◽  
Grape Seed ◽  
Oxidative Capacity ◽  
Seed Extract ◽  
Grape Seeds

Nephrolithiasis is a complex disease that results from a combination of factors related to both urine composition and kidney morphoanatomy. Development of calcium oxalate monohydrate papillary calculi is linked to initial subepithelial calcification of renal papilla. Progressive tissue calcification depends on preexisting injury and involves reactive oxygen species. Many plant extracts that protect against oxidative stress manifest antilithiasic activity. Our study focused on determining the effects of polyphenols on a lithiasis rat model. Rats were pretreated with polyphenols and grape seed extracts, followed by posterior induction of hyperoxalosis via treatment with ethylene glycol plus NH4Cl. The concentrations of calcium and other elements in kidney were determined, along with histological examination of kidney and 24 h urine analysis. Significant differences were observed in the renal calcium content between the control plus ethylene glycol-treated group and the epicatechin plus ethylene glycol-treated, red grape seed extract plus ethylene glycol-treated, and white grape seed extract plus ethylene glycol-treated groups, with reductions of about 50%. The antioxidant activity of polyphenols extracted from red and white grape seeds may be critical in the prevention of calcium oxalate monohydrate papillary calculus formation, particularly if calculi are induced by lesions caused by cytotoxic compounds with oxidative capacity.

scholarly journals Dual Anticoagulant/Antiplatelet Activity of Polyphenolic Grape Seeds Extract

Nutrients ◽  
2019 ◽  
Vol 11 (1) ◽  
pp. 93 ◽  
Author(s):  
Michal Bijak ◽  
Agnieszka Sut ◽  
Anna Kosiorek ◽  
Joanna Saluk-Bijak ◽  
Jacek Golanski
Keyword(s):  
Blood Platelets ◽  
Adenosine Diphosphate ◽  
Grape Seed Extract ◽  
Grape Seed ◽  
Potential Effect ◽  
In Vitro Models ◽  
Seed Extract ◽  
Grape Seeds ◽  
Induced Aggregation

Because of the side-effects of commonly used anti-platelet and anticoagulant drugs, investigations into plant substances with similar activities are very common. Based on our own studies in recent years, we estimate that it is possible to use natural compounds to both inhibit coagulation pathway enzymes and to reduce blood platelets’ activation. As such, in our current study we wanted to verify the anti-platelet and anticoagulant properties of grape seed extract (GSE) using in vitro models. During our analysis, the following parameters were analyzed: Coagulation times, thromboelastometry assays (coagulation time, clot formation time and maximum clot firmness), aggregation of platelets and phosphorylation of vasodilator-stimulated phosphoprotein (VASP). Adenosine diphosphate (ADP)-induced aggregation was lower in GSE 7.5 µg/mL as well as in GSE 15.0 µg/mL. A similar dependence was observed in VASP assays for GSE 7.5 µg/mL and GSE 15 µg/mL. The effect on plasma coagulation tests was distinct only with GSE 15 µg/mL. All of the thromboelastometry variables were statistically significant with 15.0 µg/mL GSE concentration. Our results show, for the first time, the multi-potential effect of grape seed extract on coagulation systems, and clearly suggest that grape seed extract could be considered a promising nutraceutical in the prevention of cardiovascular thrombotic events caused by different mechanisms.

Sign in / Sign up

Export Citation Format

Share Document