scholarly journals Effect of High-Fat Diet on Peripheral Neuropathy in C57BL/6 Mice

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Lingling Xu ◽  
Dou Tang ◽  
Meiping Guan ◽  
Cuihua Xie ◽  
Yaoming Xue
Keyword(s):  
Oxidative Stress ◽  
Peripheral Neuropathy ◽  
Nerve Fiber ◽  
High Fat Diet ◽  
Sensory Nerve ◽  
Thermal Hyperalgesia ◽  
High Fat ◽  
Fiber Density ◽  
Dendrite Length ◽  
Intraepidermal Nerve Fiber Density

Objective. Dyslipidemia may contribute to the development of peripheral neuropathy, even in prediabetics; however, few studies have evaluated vascular dysfunction and oxidative stress in patients with peripheral neuropathy.Methods. Using high-fat diet- (HFD-) induced prediabetic C57BL/6 mice, we assessed motor and sensory nerve conduction velocity (NCV) using a BIOPAC System and thermal algesia with a Plantar Test (Hargreaves’ method) Analgesia Meter. Intraepidermal nerve fiber density and mean dendrite length were tested following standard protocols. Vascular endothelial growth factor-A (VEGF-A) and 12/15-lipoxygenase (12/15-LOX) were evaluated by immunohistochemistry and Western blot, respectively.Results. HFD-fed mice showed deficits in motor and sensory NCV, thermal hyperalgesia, reduced mean dendrite length, and VEGF-A expression in the plantar skin and increased 12/15-LOX in the sciatic nerve (P<0.05compared with controls).Conclusion. HFD may cause large myelinated nerve and small sensory nerve fiber damage, thus leading to neuropathy. The mean dendrite length may be a more sensitive marker for early detection of peripheral neuropathy. Reduced blood supply to the nerves and increased oxidative stress may contribute to the development and severity of peripheral neuropathy.

scholarly journals Electroacupuncture Alleviates Diabetic Peripheral Neuropathy by Regulating Glycolipid-Related GLO/AGEs/RAGE Axis

2021 ◽  
Vol 12 ◽  
Author(s):  
Xuan Wang ◽  
Qian Li ◽  
Xu Han ◽  
Meirong Gong ◽  
Zhi Yu ◽  
...  
Keyword(s):  
Peripheral Neuropathy ◽  
Inflammatory Cytokines ◽  
Diabetic Peripheral Neuropathy ◽  
Thermal Hyperalgesia ◽  
Fiber Density ◽  
Density Reduction ◽  
Intraepidermal Nerve Fiber Density ◽  
Regulation Of Metabolism ◽  
Glycation End Products ◽  
Pro Inflammatory Cytokines

Diabetic peripheral neuropathy (DPN) is one of the most common complications of diabetes mellitus (DM) and affects over one-third of all patients. Neuropathic pain and nerve dysfunction induced by DM is related to the increase of advanced glycation end products (AGEs) produced by reactive dicarbonyl compounds in a hyperglycemia environment. AGEs induce the expression of pro-inflammatory cytokines via the main receptor (RAGE), which has been documented to play a crucial role in the pathogenesis of diabetic peripheral neuropathy. Electroacupuncture (EA) has been reported to have a positive effect on paralgesia caused by various diseases, but the mechanism is unclear. In this study, we used high-fat-fed low-dose streptozotocin-induced rats as a model of type 2 diabetes (T2DM). Persistent metabolic disorder led to mechanical and thermal hyperalgesia, as well as intraepidermal nerve fiber density reduction and nerve demyelination. EA improved neurological hyperalgesia, decreased the pro-inflammatory cytokines, reduced the generation of AGEs and RAGE, and regulated the glyoxalase system in the EA group. Taken together, our study suggested that EA plays a role in the treatment of T2DM-induced DPN, and is probably related to the regulation of metabolism and the secondary influence on the GLO/AGE/RAGE axis.

scholarly journals Effect of Dietary Content of Menhaden Oil with or without Salsalate on Neuropathic Endpoints in High-Fat-Fed/Low-Dose Streptozotocin-Treated Sprague Dawley Rats

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Eric P. Davidson ◽  
Lawrence J. Coppey ◽  
Hanna Shevalye ◽  
Alexander Obrosov ◽  
Mark A. Yorek
Keyword(s):  
Vascular Reactivity ◽  
Sensory Nerve ◽  
Diabetic Rats ◽  
Diabetic Rat ◽  
Neural Function ◽  
Menhaden Oil ◽  
High Fat ◽  
Fiber Density ◽  
Thermal Nociception ◽  
Intraepidermal Nerve Fiber Density

In this study, we wanted to extend our investigation of the efficacy of fish oil with or without salsalate on vascular and neural complications using a type 2 diabetic rat model. Four weeks after the onset of hyperglycemia, diabetic rats were treated via the diet with 3 different amounts of menhaden oil with or without salsalate for 12 weeks. Afterwards, vascular reactivity of epineurial arterioles and neuropathy-related endpoints were examined. The addition of salsalate to high-fat diets enriched with 10% or 25% kcal of menhaden oil protected vascular reactivity to acetylcholine and calcium gene-related peptide, motor and sensory nerve conduction velocity, thermal nociception, intraepidermal nerve fiber density, and cornea sensitivity to a greater extent than 10% or 25% menhaden oil alone. Vascular and neural function was maximally protected with diet containing 45% kcal as menhaden oil, and adding salsalate did not provide any additional benefit. Salsalate alone in the high-fat diet of diabetic rats provided minimal protection/improvement of vascular and neural dysfunction. These studies imply that dietary salsalate in combination with lower amounts of menhaden oil can provide greater benefit toward diabetes-induced vascular and neural impairment than menhaden oil alone.

The role of oxidative stress and inflammatory response in high-fat diet induced peripheral neuropathy

2014 ◽  
Vol 55 ◽  
pp. 51-57 ◽  
Author(s):  
Rafet Ozay ◽  
Ertugrul Uzar ◽  
Abit Aktas ◽  
Mehtap Erkmen Uyar ◽  
Bora Gürer ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Peripheral Neuropathy ◽  
Inflammatory Response ◽  
High Fat Diet ◽  
High Fat

Hesperetin ameliorates hepatic oxidative stress and inflammation via the PI3K/AKT-Nrf2-ARE pathway in oleic acid-induced HepG2 cells and a rat model of high-fat diet-induced NAFLD

2021 ◽  
Author(s):  
Jingda Li ◽  
Tianqi Wang ◽  
Panpan Liu ◽  
Fuyuan Yang ◽  
Xudong Wang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Oleic Acid ◽  
Rat Model ◽  
High Fat Diet ◽  
Hepg2 Cells ◽  
Citrus Fruits ◽  
High Fat ◽  
Hepatic Oxidative Stress

Hesperetin as a major bioflavonoid in citrus fruits improves NAFLD by suppressing hepatic oxidative stress and inflammation.

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