scholarly journals Diversity, Biocontrol, and Plant Growth Promoting Abilities of Xylem Residing Bacteria from Solanaceous Crops

2014 ◽  
Vol 2014 ◽  
pp. 1-14 ◽  
Author(s):  
Gauri A. Achari ◽  
Raman Ramesh
Keyword(s):  
Plant Growth ◽  
Restriction Analysis ◽  
Solanum Melongena ◽  
Solanum Torvum ◽  
Plant Growth Promoting Activities ◽  
Plant Growth Promoting ◽  
Xylem Tissue ◽  
Growth Promoting ◽  
Amplified Rdna Restriction Analysis

Eggplant (Solanum melongenaL.) is one of the solanaceous crops of economic and cultural importance and is widely cultivated in the state of Goa, India. Eggplant cultivation is severely affected by bacterial wilt caused byRalstonia solanacearumthat colonizes the xylem tissue. In this study, 167 bacteria were isolated from the xylem of healthy eggplant, chilli, andSolanum torvumSw. by vacuum infiltration and maceration. Amplified rDNA restriction analysis (ARDRA) grouped these xylem residing bacteria (XRB) into 38 haplotypes. Twenty-eight strains inhibited growth ofR. solanacearumand produced volatile and diffusible antagonistic compounds and plant growth promoting substancesin vitro. Antagonistic strains XB86, XB169, XB177, and XB200 recorded a biocontrol efficacy greater than 85% against BW and exhibited 12%–22 % increase in shoot length in eggplant in the greenhouse screening. 16S rRNA based identification revealed the presence of 23 different bacterial genera. XRB with high biocontrol and plant growth promoting activities were identified as strains ofStaphylococcussp.,Bacillussp.,Streptomycessp.,Enterobactersp., andAgrobacteriumsp. This study is the first report on identity of bacteria from the xylem of solanaceous crops having traits useful in cultivation of eggplant.

scholarly journals In Vitro Plant Growth Promoting Activity of Phyllocladane Diterpenoids Isolated from Callicarpa macrophylla Vahl. in Shoot Cultures of Rauwolfia serpentina

2007 ◽  
Vol 2 (8) ◽  
pp. 1934578X0700200 ◽  
Author(s):  
Manoj K Goel ◽  
Arun K Kukreja ◽  
Anil K Singh ◽  
Suman Preet S Khanuja
Keyword(s):  
Plant Growth ◽  
Shoot Cultures ◽  
Rauwolfia Serpentina ◽  
Plant Growth Promoting Activities ◽  
Plant Growth Promoting ◽  
Plant Tissue Cultures ◽  
Plant Growth Promoting Activity ◽  
Growth Promoting ◽  
In Vitro Shoot Cultures

Phyllocladane diterpenoids, particularly calliterpenone (1) and calliterpenone monoacetate (2), isolated from leaves of Callicarpa macrophylla, produced significantly higher growth and multiplication of in vitro shoot cultures of Rauwolfia serpentina at 0.25 and 0.5 mg/L concentrations, respectively, compared to certain other plant growth regulators (0.1-5.0 mg/L) tested under in vitro conditions. This is the first report of the plant growth promoting activities of 1 and 2 in plant tissue cultures.

scholarly journals Phenetic and Molecular Diversity of Nitrogen Fixating Plant Growth Promoting Azotobacter Isolated from Semiarid Regions of India

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Devendra Jain ◽  
Jyoti Sharma ◽  
Gunnjeet Kaur ◽  
Ali Asger Bhojiya ◽  
Surya Chauhan ◽  
...  
Keyword(s):  
Plant Growth ◽  
Growth Parameters ◽  
Restriction Analysis ◽  
Qualitative And Quantitative ◽  
Rrna Sequencing ◽  
Plant Growth Promoting Activities ◽  
Plant Growth Promoting ◽  
Dna Restriction ◽  
Pgp Activities ◽  
Growth Promoting

In the present study, 24 Azotobacter strains were isolated from soils of different areas of southern Rajasthan and characterized at biochemical, functional, and molecular levels. The isolated Azotobacter strains were gram negative and cyst forming when viewed under the microscope. These strains were also screened for their plant growth promoting activities and the ability of these isolates to survive under abiotic stress conditions viz. salt, pH, temperature, and drought stress. All the isolates showed IAA, siderophore, HCN, and ammonia production, whereas seven Azotobacter strains showed phosphate solubilization. Amplified Ribosomal DNA Restriction Analysis (ARDRA) revealed significant diversity among Azotobacter strains and the dendrogram obtained differentiated twenty-four of the strains into two major clusters at a similarity coefficient of 0.64. Qualitative and quantitative N2 fixation abilities of these strains were also detrained, and the amounts of acetylene reduced by Azotobacter strains were in the range of 1.31 to 846.56 nmol C2H4 mg protein−1 h−1. The strains showing high nitrogen fixation ability with multiple PGP activities were selected for further pot studies, and these Azotobacter strains significantly increased the various plant growth parameters of maize plantlets. Furthermore, the best Azotobacter isolates were subjected to 16S rRNA sequencing and confirmed their identities as Azotobacter sp. The indigenous Azotobacter strains with multiple PGP activities could be further used for commercial production.

Biocontrol of Ear Rot Fungi By Plant Growth Promoting Fluorescent Pseudomonads

2016 ◽  
Vol 1 (2) ◽  
Author(s):  
Jitendra Mishra ◽  
Malvika Rajnandani ◽  
Naveen Kumar Arora
Keyword(s):  
Plant Growth ◽  
Growth Promotion ◽  
Crop Productivity ◽  
Fluorescent Pseudomonads ◽  
Ear Rot ◽  
Plant Growth Promoting Activities ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Biocontrol Potential

In India, maize is the third most important food crop after rice and wheat but the productivity is severely affected as the plant is also prone to a number of diseases caused by fungi and bacteria. Ear rot caused by <italic>Fusarium moniliforme</italic> is one of the economically important soil and seed borne disease of maize and not easily controlled by chemical methods. Antagonistic bacteria may constitute an alternative for improving the crop productivity. In this study 13 fluorescent pseudomonads were isolated from rhizosphere of different plants and screened for plant growth promoting activities and biocontrol potential against <italic>F. moniliforme</italic> ITCC No. 2193. Among them a potential isolate, W3Gr-6(b) not only inhibited growth of <italic>F. moniliforme</italic> under in-vitro conditions but was also capable of maize growth promotion in presence of pathogen.

scholarly journals In Vitro and In Vivo Plant Growth Promoting Activities and DNA Fingerprinting of Antagonistic Endophytic Actinomycetes Associates with Medicinal Plants

PLoS ONE ◽  
2015 ◽  
Vol 10 (9) ◽  
pp. e0139468 ◽  
Author(s):  
Ajit Kumar Passari ◽  
Vineet Kumar Mishra ◽  
Vijai Kumar Gupta ◽  
Mukesh Kumar Yadav ◽  
Ratul Saikia ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Plant Growth ◽  
Dna Fingerprinting ◽  
Endophytic Actinomycetes ◽  
Plant Growth Promoting Activities ◽  
Plant Growth Promoting ◽  
Growth Promoting

scholarly journals BIO-EFFICACY OF FUNGAL BIOAGENTS AND ITS CRUDE SECONDARY ME-TABOLITES AGAINST ROOT KNOT NEMATODE MELOIDOGYNE INCOGNITA [(KOFOID & WHITE,1919) CHITWOOD, 1949]INFESTING TOMATO (SOLANUMLYCOPERSICUM L.)

Redia ◽  
2021 ◽  
Vol 104 ◽  
pp. 37-43
Author(s):  
LAKSHMAN PRASAD ◽  
RASHID PERVEZ ◽  
SWATI GABA
Keyword(s):  
Plant Growth ◽  
In Vitro Study ◽  
Good Alternative ◽  
Root Knot Nematode ◽  
Egg Hatching ◽  
Antagonistic Microorganisms ◽  
Plant Growth Promoting Activities ◽  
Plant Growth Promoting ◽  
Growth Promoting

Tomato (Solanumlycopersicum L.) is one of the most important vegetable cropin the world. Various obstacles are existingwith this cropcultivation, among them root knot nematode,Meloidogyne incognitarecognized as a major path-ogen of tomato. The complete elimination of M. incognitafromthe soil is very difficult because of its polyphagous nature. Although,different methods are employed to manage this nematode, however, biological control has become a good alternative to the chemical nematicides. Antagonistic microorganisms are appropriate for controlling nematodes still require progressive investigation. Hence,present study on the biologicalactivity of the different concentrations of mixer of crude secondary metabolitesof the various fungal bioagents againston egg hatching, M. incognita(J2) mortal-ity in vitro as well asin planta were evaluated. Plant growth promoting activities were also recorded. Among the tested fungal bioagentsV. lecanii, F. chlamydosporum andA. niger were found promising in vitroas well asin planta studies while M. anisopliaeand A. nigerwas found promising to inhibit the egg hatchingunder in vitro study. It is concluded thatV. lecanii, F. chlamydosporum andA. nigerhave high antagonistic/nematicidalas well as good plant growth pro-motion activities. These promising fungal biocontrol agents can be considering for evaluation on a larger scale in field.

scholarly journals Isolation and characterization of plant growth promoting endophytic bacteria isolated from Vigna radiata

2018 ◽  
Author(s):  
Pooja Suneja ◽  
Rajat Maheshwari ◽  
Namita Bhutani
Keyword(s):  
Plant Growth ◽  
Vigna Radiata ◽  
Endophytic Bacteria ◽  
Fungal Pathogens ◽  
Growth Promotion ◽  
Restriction Analysis ◽  
Isolation And Characterization ◽  
Plant Growth Promoting ◽  
Growth Promoting

A total of 22 endophytic bacteria were isolated from roots and nodules of Vigna radiata (mungbean) obtained from Jind district, Haryana. These were characterized on the basis of plant growth promoting traits. Almost all the endophytic bacteria produced IAA with maximum production of 81.63µg/ml by isolate MJiR8. Among these, 100% root isolates and 84.6% nodule isolates resulted in in vitro root growth promotion of mungbean seedlings. All the isolates produced ammonia; eighteen (all root and nine nodule isolates) produced organic acid while only four root isolates were positive for siderophore production. The four isolates produced hydrogen cyanide and out of these only MJiR9 inhibited the growth of fungal pathogens Fusarium oxysporium and Aspergillus niger. All the endophytes were used to determine molecular diversity by ARDRA (Amplified Ribosomal DNA Restriction Analysis) Results revealed that the nodule isolates were more diverse, being present in separate clusters, in comparison to root isolates which were grouped together in cluster III.

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