scholarly journals Synthesis of 6-(2-Methoxynaphthyl)-2,3-dihydro-1,2,4-triazine-3-thione as a New Reagent for Spectrophotometric Determination of Copper

2014 ◽  
Vol 2014 ◽  
pp. 1-6
Author(s):  
Maliheh Barazandeh Tehrani ◽  
Hutan Ghanbari ◽  
Effat Souri ◽  
Fazel Shamsa ◽  
Mohsen Amini
Keyword(s):  
Atomic Absorption ◽  
Spectrophotometric Method ◽  
Limit Of Detection ◽  
Dental Alloy ◽  
Absorption Method ◽  
Limit Of Quantification ◽  
Amyl Nitrite ◽  
Significant Difference ◽  
Determination Of Copper

A simple, sensitive, accurate, and green spectrophotometric method for the determination of Cu(II) using newly synthesized reagent, 6-(2-methoxynaphthyl)-2,3-dihydro-1,2,4-triazine-3-thione (MNDTT), has been developed. MNDTT was synthesized based on the acylation of methoxy naphthalene and reaction of the product with amyl nitrite, which upon reaction with thiosemicarbazide yielded 6-(2-meyhoxynaphthyl)-2,3-dihydro-1,2,4-triazine-3-thione. MNDTT produces a dark red complex with copper in methanol according to the 1 : 2 stoichiometry. Beer’s law was obeyed over the concentration range of 2.5–20 µg/mL withr2=0.992. The limit of detection and limit of quantification were 0.33 and 1.10 µg/mL, respectively. Within-day and between-day precision values were less than 3.68%. Finally, the method has been applied to a dental alloy (110-plus) successfully and the results were compared with atomic absorption method. The results showed that there was no significant difference between the two methods (P>0.05).

Collaborative Study Using Atomic Absorption Spectrophotometry for the Determination of Copper in Alcoholic Products

1967 ◽  
Vol 50 (2) ◽  
pp. 338-339
Author(s):  
Duane H Strunk ◽  
A A Andreasen
Keyword(s):  
Atomic Absorption ◽  
Spectrophotometric Method ◽  
Collaborative Study ◽  
Atomic Absorption Spectrophotometry ◽  
Good Precision ◽  
Absorption Method ◽  
Standard Curve ◽  
Absorption Spectrophotometry ◽  
Determination Of Copper

Abstract A collaborative study was conducted on the 'atomic absorption spectrophotometric method for measuring the concentration of copper in alcoholic products. In this method, the samples are aspirated directly into the burner of the instrument, and the absorhance values are converted to ppm copper by reference to a standard curve. Data show good precision and are comparable to those obtained by the ZDBT method. It is recommended that the atomic absorption method be adopted as official, first action.

scholarly journals DEVELOPMENT AND VALIDATION OF UV-SPECTROPHOTOMETRIC METHOD FOR ESTIMATION OF HYDROQUINONE IN BULK, MARKETED CREAM AND PREAPARED NLC FORMULATION

2017 ◽  
Vol 9 (5) ◽  
pp. 102
Author(s):  
Sukhjinder Kaur ◽  
Taranjit Kaur ◽  
Gurdeep Kaur ◽  
Shivani Verma
Keyword(s):  
Spectrophotometric Method ◽  
Limit Of Detection ◽  
Pharmaceutical Formulations ◽  
Pure Form ◽  
Nanostructured Lipid Carrier ◽  
Limit Of Quantification ◽  
Double Beam ◽  
Uv Visible ◽  
Development And Validation

Objective: The aim of the present work was to develop a simple, rapid, accurate and economical UV-visible spectrophotometric method for the determination of hydroquinone (HQ) in its pure form, marketed formulation as well as in the prepared nanostructured lipid carrier (NLC) systems and to validate the developed method.Methods: HQ was estimated at UV maxima of 289.6 nm in pH 5.5 phosphate buffer using UV-Visible double beam spectrophotometer. Following the guidelines of the International Conference on Harmonization (ICH), the method was validated for various analytical parameters like linearity, precision, and accuracy robustness, ruggedness, limit of detection, quantification limit, and formulation analysis.Results: The obtained results of the analysis were validated statistically. Recovery studies were performed to confirm the accuracy of the proposed method. In the developed method, linearity over the concentration range of 5-40 μg/ml of HQ was observed with the correlation coefficient of 0.998 and found in good agreement with Beer Lambert’s law. The precision (intra-day and inter-day) of the method was found within official RCD limits (RSD<2%).Conclusion: The sensitivity of the method was assessed by determining the limit of detection and limit of quantification. It could be concluded from the results obtained that the purposed method for estimation of HQ in pure form, in the marketed ointment and in the prepared NLC-formulation was simple, rapid, accurate, precise and economical. It can be used successfully in the quality control of pharmaceutical formulations and for the routine laboratory analysis.

HPTLC Separation of a Hepatoprotective Combination in Pharmaceutical Formulation and Human Plasma

2020 ◽  
Vol 58 (5) ◽  
pp. 411-417
Author(s):  
Maimana A Magdy ◽  
Rehab M Abdelfatah
Keyword(s):  
Human Plasma ◽  
High Performance ◽  
Limit Of Detection ◽  
Pharmaceutical Formulation ◽  
Limit Of Quantification ◽  
Ich Guidelines ◽  
Spiked Plasma ◽  
Significant Difference ◽  
Developing System

Abstract A binary mixture of Silymarin (SR) and Vitamin E (VE) acetate, of an antioxidant and a hepatoprotective effect, has been analyzed using a sensitive, selective and economic high performance thin layer chromatographic (HPTLC) method in their pure forms, pharmaceutical formulation and spiked human plasma. SR and VE were separated on 60F254 silica gel plates using hexane:acetone:formic acid (7:3:0.15, v/v/v) as a developing system with UV detection at 215 nm. The method was evaluated for linearity, accuracy, precision, selectivity, limit of detection (LOD) and limit of quantification (LOQ). SR and VE were detected in the linear range of 0.2–2.5 and 0.2–4.5 μg/band, respectively. Method validation was done as per ICH guidelines and acceptable results of accuracy of 99.86 ± 1.190 and 100.22 ± 1.609 for SR and VE, respectively were obtained. The method has been successfully applied for determination of the studied drugs in their pharmaceutical formulation without any interference from excipients, and in spiked plasma samples. Results obtained by the developed HPTLC-densitometric method were statistically compared to those obtained by the reported HPLC methods and no significant difference was found between them.

scholarly journals A simple spectrophotometric method for the determination of methyldopa using p-chloranil in the presence of hydrogen peroxide

2008 ◽  
Vol 33 (3) ◽  
pp. 7-12 ◽  
Author(s):  
M. A. Gotardo ◽  
L. S. Lima ◽  
R. Sequinel ◽  
J. L. Rufino ◽  
L. Pezza ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Spectrophotometric Method ◽  
Limit Of Detection ◽  
Pharmaceutical Formulations ◽  
Limit Of Quantification ◽  
Coefficients Of Variation ◽  
The Common ◽  
Interday Precision ◽  
Sensitive Spectrophotometric Method

A simple, rapid and sensitive spectrophotometric method has been developed for the determination of methyldopa in pharmaceutical formulations. The method is based on the reaction between tetrachloro-p-benzoquinone (p-chloranil) and methyldopa, accelerated by hydrogen peroxide (H2O2), producing a violet-red compound (λmax = 535 nm) at ambient temperature (25.0 ± 0.2 ºC). Experimental design methodologies were used to optimize the measurement conditions. Beer's law is obeyed in a concentration range from 2.10 x 10-4 to 2.48 x 10-3 mol L-1 (r = 0.9997). The limit of detection was 7.55 x 10-6 mol L-1 and the limit of quantification was 2.52 x 10-5 mol L-1. The intraday precision and interday precision were studied for 10 replicate analyses of 1.59 x 10-3 mol L-1 methyldopa solution and the respective coefficients of variation were 0.7 and 1.1 %. The proposed method was successfully applied to the determination of methyldopa in commercial brands of pharmaceuticals. No interferences were observed from the common excipients in the formulations. The results obtained by the proposed method were favorably compared with those given by the Brazilian Pharmacopoeia procedure at 95 % confidence level.

scholarly journals Third Derivative Spectrophotometric Method for Simultaneous Determination of Copper and Nickel Using 6-(2-Naphthyl)-2, 3-dihydro-1,2,4- triazine-3-thione

2011 ◽  
Vol 8 (2) ◽  
pp. 587-593 ◽  
Author(s):  
Maliheh Barazandeh Tehrani ◽  
Effat Souri
Keyword(s):  
Simultaneous Determination ◽  
Spectrophotometric Method ◽  
Limit Of Detection ◽  
Alloy Sample ◽  
Zero Crossing ◽  
Determination Of Nickel ◽  
Analytical Reagent ◽  
Third Derivative ◽  
Determination Of Copper

A simple and sensitive derivative spectrophotometric method for simultaneous determination of nickel and copper using 6-(2- naphthyl)-2,3-dihydro-1,2,4-triazine-3-thione (NDTT) as a selective analytical reagent was developed. The complexes of metal ions with NDTT were formed immediately in basic media and extracted with chloroform. The zero-crossing measurement technique was found suitable for the direct measurement of the third-derivative value of Ni-NDTT and Cu-NDTT at 501 and 472 nm respectively. Beer’s law is obeyed in the concentration range 1-30 μg/mL for both cations with different ratios. The limit of detection was 0.26 μg/mL and 0.13 μg/mL for copper and nickel respectively. The within day and between day variations showed coefficient of variation (CV%) values less than 2.80 and 2.97 for copper and nickel respectively. The error of the determination method did not exceed 6.50%. Analysis of alloy sample showed that this method can be successfully used for simultaneous determination of Cu and Ni in real samples.

scholarly journals Ultraviolet Spectrophotometric Method for Determination of Glipizide in Presence of Liposomal/Proliposomal Turbidity

2013 ◽  
Vol 2013 ◽  
pp. 1-5
Author(s):  
Neelkant Prasad ◽  
Roshan Issarani ◽  
Badri Prakash Nagori
Keyword(s):  
Spectrophotometric Method ◽  
Calibration Curve ◽  
Limit Of Detection ◽  
Quantitative Estimation ◽  
Uv Detection ◽  
Limit Of Quantification ◽  
Relative Standard ◽  
Precision And Accuracy ◽  
Clear Solution

A simple and sensitive ultraviolet spectrophotometric method for quantitative estimation of glipizide in presence of lipid turbidity is described to avoid false estimation due to diffraction by turbidity. UV detection was performed at 230 nm, 225 nm, and 235 nm, and the calibration curve was plotted between resultant of absorbance of [230 nm − (225 nm + 235 nm)/2] and concentration of analyte. The calibration curve was linear over the concentration range tested (1–20 μg/mL) with limit of detection of 0.27 μg/mL and limit of quantification of 0.82 μg/mL. Percent relative standard deviations and percent relative mean error, representing precision and accuracy, respectively, for clear as well as turbid solutions, were found to be within acceptable limits, that is, always less than 0.69 and 0.41, respectively, for clear solution and 0.65 and 0.47, respectively, for turbid solution. Conclusively, our method was successfully applied for the determination of glipizide in clear as well as turbid solutions, and it was found that the drug analyte in both types of solutions can be detected from the same calibration curve accurately and precisely and glipizide entrapped in the liposomes or in proliposomal matrix was not detected.

scholarly journals Anodic Stripping Voltammetric Determination Of Copper In Multivitamin-Mineral Formulations Using Iodine-Coated Platinum Electrode

2021 ◽  
Vol 16 (1) ◽  
pp. 48-56
Author(s):  
Mohammad Amayreh ◽  
Wafa Hourani ◽  
Mohammed Khair Hourani
Keyword(s):  
Platinum Electrode ◽  
Limit Of Detection ◽  
Statistical Tests ◽  
Supporting Electrolyte ◽  
Copper Oxidation ◽  
Copper Determination ◽  
Limit Of Quantitation ◽  
Significant Difference ◽  
Determination Of Copper

In this work, the utilization of the modified iodine-coated polycrystalline platinum electrode as a voltammetric sensor for copper determination in pharmaceutical formulations was reported. The optimized experimental parameters for the determination of copper were using 0.1M KCl as a supporting electrolyte with a scan rate of 50 mV/s, deposition potential of - 0.2 V, and a deposition time of 2 minutes. The anodic peak related to Copper oxidation is centered at about + 0.05 V. The extended detected linear range for the developed method was between 1 ppm and 100 ppm. The anodic current showed excellent linearity with R2 = 0.9986. The limit of detection (LOD) and limit of quantitation (LOQ) were 0.115 ppm and 0.346 ppm, respectively, which attests to the sensitivity of the method. The investigation for the effect of potential interferences from multivitamins tablet ingredients indicated a specific selectivity toward copper and the absence of any electrochemical response toward these components. The developed method was successfully applied to analyze copper and the obtained results were in good agreement with the labeled values, besides that, the statistical tests indicated no significant difference at p = 0.05 with 95 % confidence level.

scholarly journals Methodological aspects concerning sampling and determination of total selenium and selenium species in geothermal waters

2020 ◽  
Vol 18 (1) ◽  
pp. 5-16 ◽  
Author(s):  
Piotr Rusiniak ◽  
Anna Ruszczyńska ◽  
Katarzyna Wątor ◽  
Ewa Bulska ◽  
Ewa Kmiecik
Keyword(s):  
Limit Of Detection ◽  
Selenium Concentration ◽  
Geothermal Water ◽  
Limit Of Quantification ◽  
Geothermal Waters ◽  
Selenium Species ◽  
Icp Ms ◽  
Significant Difference ◽  
Total Selenium

AbstractThe work presents the results of geothermal water sample analysis with respect to the determination of total selenium concentration. For this purpose, geothermal water (GT) samples were collected from three different intakes (GT-1, GT-2, GT-3) with similar temperatures of about 85°C. Tests were carried out to see if the methodology of sample preparation influenced total selenium concentration during analysis by inductively coupled plasma mass spectrometry (ICP-MS). Samples (raw and filtered) were preserved with nitric acid (HNO3) and mineralised in the laboratory. From the data obtained it was found that there is no significant difference between total selenium concentration in raw and filtered samples. Following mineralisation, the concentrations in the samples were below the limit of detection or limit of quantification. While different analytical steps or procedures are applied, the results of total selenium concentration can vary. Furthermore, high-performance liquid chromatography coupled with ICP-MS was used for the identification of selenium species. The results revealed that hexavalent selenium – Se(VI) – in the geothermal water was found only in samples collected from the GT-2 and GT-3 intakes.

scholarly journals ULTRAVIOLET-SPECTROPHOTOMETRIC METHOD DEVELOPMENT AND VALIDATION FOR THE DETERMINATION OF NORFLOXACIN PRESENT IN TASTE MASKED DRUG RESIN COMPLEX

2018 ◽  
Vol 11 (4) ◽  
pp. 244
Author(s):  
Ayya Rajendra Prasad ◽  
Jayanthi Vijaya Ratna
Keyword(s):  
Spectrophotometric Method ◽  
Absorption Maximum ◽  
Method Development ◽  
Limit Of Detection ◽  
Statistical Parameters ◽  
Limit Of Quantification ◽  
Relative Standard ◽  
Validation Parameters ◽  
Development And Validation

 Objective: The objective of this study was developed and validated a novel, specific, precise, and simple ultraviolet (UV)-spectrophotometric method for the estimation of norfloxacin present in taste masked drug-resin complex.Methods: UV-spectrophotometric determination was performed with ELICO SL 1500 UV-visible spectrophotometer using 0.1 N HCl as a medium. The spectrum of the standard solution was run from 200 to 400 nm range for the determination of absorption maximum (λ max). λ max of norfloxacin was found at 278 nm. The absorbance of standard solutions of 1, 2, 3, 4, and 5 μg/ml of drug solution was measured at an absorption maximum at 278 nm against the blank. Then, a graph was plotted by taking concentration on X-axis and absorbance on Y-axis which gave a straight line. Validation parameters such as linearity and range, selectivity and specificity, limit of detection (LOD) and limit of quantification (LOQ), accuracy, precision, and robustness were evaluated as per the International Conference on Harmonization (ICH) guidelines.Results: Linearity for the UV-spectrophotometric method was noted over a concentration range of 1–5 μg/ml with a correlation coefficient of 0.9995. The LOD and LOQ for norfloxacin were found at 0.39 μg/ml and 1.19 μg/ml, respectively. Accuracy was in between 99.00% and 99.17%. % relative standard deviation for repeatability, intraday precision, and interday precision was found to be 0.600, in between 0.291 and 0.410, and in between 0.682 and 1.439, respectively. The proposed UV spectrophotometric method is found to be robust.Conclusion: The proposed UV-spectrophotometric method was validated according to the ICH guidelines, and results and statistical parameters demonstrated that the developed method is sensitive, precise, reliable, and simple for the estimation of norfloxacin present in taste masked drug-resin complex.

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