scholarly journals Hypocholesterolemic Activity ofMonascusFermented Product in the Absence of Monacolins with Partial Purification for Functional Food Applications

2014 ◽  
Vol 2014 ◽  
pp. 1-12 ◽  
Author(s):  
Zahra Ajdari ◽  
Maaruf Abd Ghani ◽  
Mohd Khan Ayob ◽  
Saadi Bayat ◽  
Mazlin Mokhtar ◽  
...  
Keyword(s):  
Functional Food ◽  
Solid Phase ◽  
Ethanolic Extract ◽  
Polar Fraction ◽  
Partial Purification ◽  
Active Fraction ◽  
Coronary Vasodilator ◽  
Fermented Product ◽  
Food Applications ◽  
Bioactive Fraction

Hypercholesterolemia is one of the most common chronic diseases in human. Along with chemical therapy traditional medication is used as hypocholesterolemic remedy, however, with unfavorable side effects. Recently,Monascusfermented product (MFP) has become a popular hypocholesterolemic natural supplement. In the present study, the hypocholesterolemic activity ofMonascus purpureusFTC5391 fermented product ethanolic extract (MFPe) was investigated in hypercholesterolemic rats. Results showed that MFPe not only reduced the serum total cholesterol (TC), LDL-C, TG concentration, and TC/HDL-C ratio but also increased the HDL-C. Further, solid phase extraction (SPE) was carried out to obtain the hypocholesterolemic bioactive fraction. The high polar fraction of SPE increased the HDL-C (42%) and decreased the TC (53.3%), LDL-C (47%), and TG (50.7%) levels as well as TC/HDL-C ratio (69.1%) in serum. The GC-MS results of the active fraction revealed two main compounds, isosorbide and erythritol, which act as coronary vasodilator compounds.

Multilayer co-encapsulation of probiotics and γ-amino butyric acid (GABA) using ultrasound for functional food applications

LWT ◽  
2021 ◽  
pp. 111432
Author(s):  
Pooja Pandey ◽  
Srinivas Mettu ◽  
Hari Niwas Mishra ◽  
Muthupandian Ashokkumar ◽  
Gregory J.O. Martin
Keyword(s):  
Butyric Acid ◽  
Functional Food ◽  
Amino Butyric Acid ◽  
Food Applications

scholarly journals Apolar Bioactive Fraction ofMelipona scutellarisGeopropolis onStreptococcus mutansBiofilm

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Marcos Guilherme da Cunha ◽  
Marcelo Franchin ◽  
Lívia Câmara de Carvalho Galvão ◽  
Bruno Bueno-Silva ◽  
Masaharu Ikegaki ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Biofilm Formation ◽  
Microbial Population ◽  
Ethanolic Extract ◽  
Vehicle Control ◽  
Sem Images ◽  
Killing Assay ◽  
Bioactive Fraction ◽  
Biofilm Matrix ◽  
Scanning Electron

The aim of this study was to evaluate the influence of the bioactive nonpolar fraction of geopropolis onStreptococcus mutansbiofilm. The ethanolic extract ofMelipona scutellarisgeopropolis was subjected to a liquid-liquid partition, thus obtaining the bioactive hexane fraction (HF) possessing antimicrobial activity. The effects of HF onS. mutansUA159 biofilms generated on saliva-coated hydroxyapatite discs were analyzed by inhibition of formation, killing assay, and glycolytic pH-drop assays. Furthermore, biofilms treated with vehicle control and HF were analyzed by scanning electron microscopy (SEM). HF at 250 μg/mL and 400 μg/mL caused 38% and 53% reduction in the biomass of biofilm, respectively, when compared to vehicle control (P<0.05) subsequently observed at SEM images, and this reduction was noticed in the amounts of extracellular alkali-soluble glucans, intracellular iodophilic polysaccharides, and proteins. In addition, theS. mutansviability (killing assay) and acid production by glycolytic pH drop were not affected (P>0.05). In conclusion, the bioactive HF of geopropolis was promising to control theS. mutansbiofilm formation, without affecting the microbial population but interfering with its structure by reducing the biochemical content of biofilm matrix.

Sustainable production of natural phenolics for functional food applications

2019 ◽  
Vol 57 ◽  
pp. 233-254 ◽  
Author(s):  
Rita Mark ◽  
Xiaomei Lyu ◽  
Jaslyn J.L. Lee ◽  
Roberto Parra-Saldívar ◽  
Wei Ning Chen
Keyword(s):  
Functional Food ◽  
Sustainable Production ◽  
Food Applications

scholarly journals Purification and Characterization of Bioactive Compounds Extracted from Suaeda Maritima Leaf and its Impact on Pathogenicity of Pseudomonas Aeruginosa in Catla Catla Fingerlings

2021 ◽  
Author(s):  
Beulah G ◽  
Divya D ◽  
Sampath Kumar N.S. ◽  
Sravya M.V.N. ◽  
Govinda Rao K ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
In Vivo Studies ◽  
Catla Catla ◽  
Active Fraction ◽  
Efficient Treatment ◽  
Suaeda Maritima

Abstract Incidence of various dreadful microbial infections and the development of antibiotic resistance by infection causative microbes are the main reasons for reducing aquaculture productivity. Hence, there is an immense need for the discovery of alternative and efficient treatment for quick recovery of diseased fishes. In the present study, Suaeda maritima leaf extracts (hexane, diethyl ether, ethanol, and water) were screened for in vitro and in vivo antibacterial and antioxidant activities. Out of all the four extracts, ethanolic extract showed highest antibacterial activity against S.aureus (4.9±1.3 mm), B.subtilis (1.6±0.3 mm), K.pneumonia (4.2±1.8 mm), and P.aeruginosa (4.1±1.2 mm). Similarly, antioxidant activity was also higher for ethanolic extract (500 µg/ml) based on DPPH radical scavenging ability (71.6±1.4 %) and reducing potential (149 μg/mL) assays. Further, ethanolic extract was purified consecutively via column chromatography and preparative TLC where an active fraction was selected based on highest antibacterial (10.1±1.4 mm) and antioxidant properties (82.3±2.8 %). Active fraction was loaded onto mass spectroscopy and identified the presence of four active constituents such as 1,2,9,10-tetramethoxy-6-methyl-5,6,6a,7-tetrahydro-4H-dibenzo[de,g]quinolin-3-yl) methanol; 3',7-Dimethoxy-3-hydroxyflavone; Saponin and (19R)9acetyl19hydroxy10,14dimethyl20oxopentacyclo[11.8.0.0<2,10>.0<4,9>.0<14,19>]henicos-17-yl-acetate. Besides, in vivo studies were conducted on Catla catla fingerlings infected with P. aeruginosa under laboratory conditions. The fingerlings were segregated into 5 groups, among which group 4 and 5 were treated with crude and purified extracts. Both the extracts were efficient in treating infected fingerlings and recorded 100% survival rate which is even better than group-3 treated with a synthetic antibiotic (77%). Hence, S. maritima leaf extract can be considered as a possible alternative medicine in aquaculture

Chemically-mediated sexual display postures in pre-ovulatory female topmouth gudgeon, Pseudorasbora parva

Behaviour ◽  
2012 ◽  
Vol 149 (9) ◽  
pp. 1003-1018 ◽  
Author(s):  
D. Burnard ◽  
P.C. Hubbard ◽  
C.T. Müller ◽  
S.W. Griffiths ◽  
D. Andreou ◽  
...  
Keyword(s):  
High Performance ◽  
Solid Phase ◽  
Full Range ◽  
Common Factor ◽  
Active Fraction ◽  
Specific Chemical ◽  
Body Postures ◽  
Topmouth Gudgeon ◽  
Sexual Display ◽  
Electrophysiological Responses

While mating systems vary considerably throughout the animal kingdom, smell is increasingly recognised as a common factor in successful copulation. However, the full range of olfactory- induced mating behaviours among animals is undoubtedly underestimated in comparison to visual or contact stimuli, and this underestimation suggests that our understanding of courtship rituals may be incomplete. Here, we use the highly invasive topmouth gudgeon, Pseudorasbora parva as a biological model to separate and validate the induction of headstands as courtship behaviour in many fish species. Conspecific odour was isolated using solid phase extraction (SPE) and fractionated using high-performance liquid chromatography (HPLC). Active fractions were characterised using nuclear magnetic resonance (NMR). Electrophysiological responses of pre-ovulatory females to conspecific odour were also tested via the electro-olfactogram (EOG). Results revealed that pre-ovulatory females adopted headstand body postures during exposure to nest guarding male odour (4/12) and SPE extract (5/12) but did not display during exposure to any other odour. Fractions from HPLC yielded 1 active fraction that induced headstand body postures in pre-ovulatory females (4/12). The active fraction eluted at 30–35 min and showed no response in the UV. NMR imaging revealed low intensity levels of aliphatic protons: methylene protons (CH2) at around 2 parts per million (ppm) and methin (CH) at around 3 ppm. EOG response amplitudes were approximately double in response to pre-ovulatory female odour than to odour derived from post-ovulatory females (Mann–Whitney U-test, , ). This study provides evidence of sex-specific chemical cues and responsive, adaptive sexual behaviour in P. parva and demonstrates that female display postures may be induced by chemical stimuli alone.

scholarly journals Pilot Study: Nutritional and Preclinical Safety Investigation of Fermented Hispidin-Enriched Sanghuangporus sanghuang Mycelia: A Promising Functional Food Material to Improve Sleep

2022 ◽  
Vol 8 ◽  
Author(s):  
I-Chen Li ◽  
Fang-Chia Chang ◽  
Ching-Chuan Kuo ◽  
Hsin-Tung Chu ◽  
Tsung-Ju Li ◽  
...  
Keyword(s):  
Pilot Study ◽  
Rem Sleep ◽  
Functional Food ◽  
Ethanolic Extract ◽  
Male Rats ◽  
Feed Consumption ◽  
Sprague Dawley ◽  
Food Material ◽  
Nrf2 Signaling Pathway ◽  
Preclinical Safety

Sleep disturbances have been the hallmark of the recent coronavirus disease 2019 pandemic. Studies have shown that once sleep is disrupted, it can lead to psychological and physical health issues which can, in turn, disrupt circadian rhythm and induce further sleep disruption. As consumers are trying to establish healthy routines, nutritional and preclinical safety investigation of fermented hispidin-enriched Sanghuangporus sanghuang mycelia (GKSS) as a novel food material for spontaneous sleep in Sprague-Dawley rats is conducted for the first time. Results showed that the nutritional analysis of GKSS including moisture, ash, crude lipid, crude protein, carbohydrate, and energy were found to be 2.4 ± 0.3%, 8.0 ± 2.5%, 1.7 ± 0.3%, 22.9 ± 1.2%, 65.1 ± 3.1%, and 367.1 ± 10.2 kcal/100 g respectively. In the 28-day repeated-dose oral toxicity study, only Sprague-Dawley male rats receiving 5 g/kg showed a slight decrease in feed consumption at week 3, but no associated clinical signs of toxicity or significant weight loss were observed. Although a significant reduction of the platelet count was found in mid- and high-dose GKSS treated male groups, such changes were noted to be within the normal range and were not correlated with relative spleen weight changes. Hence, the no observed adverse effect level (NOAEL) of GKSS was identified to be higher than 5 g/kg in rats. After the safety of GKSS is confirmed, the sleep-promoting effect of GKSS ethanolic extract enriched with hispidin was further assessed. Despite 75 mg/kg of GKSS ethanolic extract does not affect wakefulness, rapid eye movement (REM) sleep and non-REM (NREM) sleep, GKSS ethanolic extract at 150 mg/kg significantly decreased wakefulness and enhanced NREM and REM sleep. Interestingly, such effects seem to be mediated through anti-inflammatory activities via NF-E2-related factor-2 (Nrf2) signaling pathway. Taken together, these findings provide the preliminary evidence to studies support the claims suggesting that GKSS contained useful phytochemical hispidin could be considered as and is safe to use as a functional food agent or nutraceutical for relieving sleep problems mediated by Nrf2 pathway, which the results are useful for future clinical pilot study.

scholarly journals Allelopathic Potential of Ethanolic Extract and Phytochemical Analysis of Paspalum maritimum Trind

2019 ◽  
Vol 37 ◽  
Author(s):  
J.C. PEREIRA ◽  
C.L.A. PAULINO ◽  
L. ENDRES ◽  
A.E.G. SANTANA ◽  
F.R.S. PEREIRA ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Condensed Tannins ◽  
Ethanolic Extract ◽  
Phytochemical Analysis ◽  
Allelopathic Effect ◽  
Active Fraction ◽  
Allelopathic Potential ◽  
Plant Parts ◽  
Allelopathic Effects ◽  
Donor Species

ABSTRACT: Allelopathy is defined as the ability of certain plants and microorganisms to interfere with the metabolism of other species through substances released into the environment, being an alternative to control weeds and diseases. In this context, this study aimed to evaluate the allelopathic potential and identify groups of secondary metabolites of Paspalum maritimum Trind. The species Lactuca sativa, Digitaria insularis, Emilia coccinea, and Portulaca oleracea were used as recipient plants. The ethanolic extract was obtained from shoot and root of donor species to evaluate the allelopathic potential. Potentially allelopathic effects were evaluated by means of germination tests, germination speed index, and initial seedling growth. A phytochemical analysis of the extract was also performed to identify the secondary metabolites. The ethanolic extract from both plant parts had an allelopathic effect on recipient species. The presence of condensed tannins, chalcones and aurones, flavonones, steroids, and saponins were detected in the most active fraction of the shoot of P. maritimum.

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