scholarly journals Biofilm Formation byMycobacterium bovis: Influence of Surface Kind and Temperatures of Sanitizer Treatments on Biofilm Control

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Victoria O. Adetunji ◽  
Aderemi O. Kehinde ◽  
Olayemi K. Bolatito ◽  
Jinru Chen
Keyword(s):  
Stainless Steel ◽  
Ammonium Chloride ◽  
Mycobacterium Bovis ◽  
Biofilm Formation ◽  
Binding Assay ◽  
Liver Extract ◽  
Hot Water ◽  
Solid Surfaces ◽  
Pine Oil ◽  
Steel Surfaces

Mycobacterium boviscauses classic bovine tuberculosis, a zoonosis which is still a concern in Africa. Biofilm forming ability of twoMycobacterium bovisstrains was assessed on coupons of cement, ceramic, or stainless steel in three different microbiological media at 37°C with agitation for 2, 3, or 4 weeks to determine the medium that promotes biofilm. Biofilm mass accumulated on coupons was treated with 2 sanitizers (sanitizer A (5.5 mg L−1active iodine) and sanitizer B (170.6 g1alkyl dimethylbenzyl ammonium chloride, 78 g−1didecyldimethyl ammonium chloride, 107.25 g L−1glutaraldehyde, 146.25 g L−1isopropanol, and 20 g L−1pine oil) at 28 and 45°C and in hot water at 85°C for 5 min. Residual biofilms on treated coupons were quantified using crystal violet binding assay. The two strains had a similar ability to form biofilms on the three surfaces. More biofilms were developed in media containing 5% liver extract. Biofilm mass increased as incubation time increased till the 3rd week. More biofilms were formed on cement than on ceramic and stainless steel surfaces. Treatment with hot water at 85°C reduced biofilm mass, however, sanitizing treatments at 45°C removed more biofilms than at 28°C. However, neither treatment completely eliminated the biofilms. The choice of processing surface and temperatures used for sanitizing treatments had an impact on biofilm formation and its removal from solid surfaces.

Biofilms Formed by Mycobacterium tuberculosis on Cement, Ceramic, and Stainless Steel Surfaces and Their Controls

2014 ◽  
Vol 77 (4) ◽  
pp. 599-604 ◽  
Author(s):  
VICTORIA ADETUNJI ◽  
ADEREMI KEHINDE ◽  
OLAYEMI BOLATITO ◽  
JINRU CHEN
Keyword(s):  
Stainless Steel ◽  
Mycobacterium Tuberculosis ◽  
Incubation Time ◽  
Liver Extract ◽  
Tween 80 ◽  
Hot Water ◽  
The Third ◽  
Steel Surfaces ◽  
Biofilm Development

This study assessed the biofilms formed by selected strains of Mycobacterium tuberculosis and investigated the efficacy of three different treatments to control the biofilms. Two M. tuberculosis strains were inoculated separately in 150 ml of Middlebrook 7H9–Tween 80 (0.1%) broth with 5% liver extract and 10% oleic albumin dextrose catalase (OADC) supplement, 5% liver extract alone, or 10% OADC alone in sterile jars, each containing a 2-cm2 coupon of cement, ceramic, or stainless steel for biofilm development at 37°C, with agitation for 2, 3, or 4 weeks. Biofilms on the coupons were exposed to 10 ml of 2% sanitizer A or 0.5% sanitizer B at 28 and 45°C and to hot water at 85°C for 5 min. Residual biofilms on treated and untreated coupons were assessed. Both strains of M. tuberculosis formed biofilms on the three surfaces; however, one strain formed more biofilms. More biofilms were formed when media containing 5% liver extract was used. Biofilm mass increased as incubation time increased until the third week. More biofilms were formed on cement than on ceramic and stainless steel coupons. Sanitizing treatments at 45°C removed more biofilms than those at 28°C. However, neither treatment completely eliminated the biofilms.

scholarly journals Salmonella Enteritidis forms biofilm under low temperatures on different food industry surfaces

2019 ◽  
Vol 49 (7) ◽  
Author(s):  
Bruna Webber ◽  
Amauri Picollo de Oliveira ◽  
Emanuele Serro Pottker ◽  
Luciane Daroit ◽  
Rafael Levandowski ◽  
...  
Keyword(s):  
Stainless Steel ◽  
Peracetic Acid ◽  
Low Temperatures ◽  
Biofilm Formation ◽  
Food Industry ◽  
Salmonella Enteritidis ◽  
Food Preservation ◽  
Hot Water ◽  
Influence Of Temperature On ◽  
Steel Surfaces

ABSTRACT: We evaluated the influence of temperature on the ability of Salmonella Enteritidis (SE) to form biofilms on stainless steel, polyethylene, and polyurethane surfaces under different hygiene procedures. These materials were placed on SE culture and incubated at 42±1 ºC, 36±1 ºC, 25±1 ºC, 9±1 ºC, and 3±1 ºC for 4, 8, 12, and 24 h. Hot water at 45 ºC and 85 ºC, 0.5% peracetic acid solution, and 1% quaternary ammonia were used for hygienization. Biofilm formation occurred at all temperatures evaluated, highlighting at 3 ºC which has not been reported as an ideal temperature for the adhesion of SE to these materials. The SE adhered more often to polyethylene surfaces than to polyurethane and stainless steel surfaces (P<0.05). Peracetic acid and water at 85 ºC had similar hygienization efficiency (P<0.05) followed by quaternary ammonia whereas water at 45 ºC was not effective. SE adhered to these materials under low temperatures which to date have been deemed safe for food preservation.

Nanoengineered Superhydrophobic Surfaces to Prevent Adhesion of Listeria monocytogenes for Improved Food Safety

2020 ◽  
Vol 63 (5) ◽  
pp. 1401-1407
Author(s):  
Bog Eum Lee ◽  
Youngsang You ◽  
Won Choi ◽  
Eun-mi Hong ◽  
Marisa M. Wall ◽  
...  
Keyword(s):  
Stainless Steel ◽  
Listeria Monocytogenes ◽  
Biofilm Formation ◽  
Electrochemical Etching ◽  
Contact Angles ◽  
Bacterial Attachment ◽  
Superhydrophobic Surfaces ◽  
List Type ◽  
Ptfe Film ◽  
Steel Surfaces

HighlightsNanoporous superhydrophobic surfaces were fabricated using electrochemical etching and Teflon coating.Adhesion of Listeria monocytogenes to the nanoengineered stainless steel surfaces was reduced.Self-cleanable food-contact surfaces prevent bacterial attachment and subsequent biofilm formation.Abstract. Bacterial attachment on solid surfaces and subsequent biofilm formation is a significant problem in the food industry. Superhydrophobic surfaces have potential to prevent bacterial adhesion by minimizing the contact area between bacterial cells and the surface. In this study, stainless steel-based superhydrophobic surfaces were fabricated by manipulating nanostructures with electrochemical etching and polytetrafluoroethylene (PTFE) film. The formation of nanostructures on stainless steel surfaces was characterized by field emission scanning electron microscopy (FESEM). The stainless steel surfaces etched at 10 V for 5 min and at 10 V for 10 min with PTFE deposition resulted in average water contact angles of 154° ±4° with pore diameters of 50 nm. In addition, adhesion of Listeria monocytogenes was decreased by up to 99% compared to the bare substrate. These findings demonstrate the potential for the development of antibacterial surfaces by combining nanoporous patterns with PTFE films. Keywords: Electrochemical etching, PTFE, Nanoengineered surface, L. monocytogenes, Superhydrophobic.

scholarly journals N-Acetyl-l-Cysteine Affects Growth, Extracellular Polysaccharide Production, and Bacterial Biofilm Formation on Solid Surfaces

2003 ◽  
Vol 69 (8) ◽  
pp. 4814-4822 ◽  
Author(s):  
Ann-Cathrin Olofsson ◽  
Malte Hermansson ◽  
Hans Elwing
Keyword(s):  
Stainless Steel ◽  
Bacterial Adhesion ◽  
Biofilm Formation ◽  
Paper Mill ◽  
Bacterial Biofilm ◽  
Extracellular Polysaccharides ◽  
Positive Effects ◽  
Initial Adhesion ◽  
Interesting Candidate ◽  
Steel Surfaces

ABSTRACT N-Acetyl-l-cysteine (NAC) is used in medical treatment of patients with chronic bronchitis. The positive effects of NAC treatment have primarily been attributed to the mucus-dissolving properties of NAC, as well as its ability to decrease biofilm formation, which reduces bacterial infections. Our results suggest that NAC also may be an interesting candidate for use as an agent to reduce and prevent biofilm formation on stainless steel surfaces in environments typical of paper mill plants. Using 10 different bacterial strains isolated from a paper mill, we found that the mode of action of NAC is chemical, as well as biological, in the case of bacterial adhesion to stainless steel surfaces. The initial adhesion of bacteria is dependent on the wettability of the substratum. NAC was shown to bind to stainless steel, increasing the wettability of the surface. Moreover, NAC decreased bacterial adhesion and even detached bacteria that were adhering to stainless steel surfaces. Growth of various bacteria, as monocultures or in a multispecies community, was inhibited at different concentrations of NAC. We also found that there was no detectable degradation of extracellular polysaccharides (EPS) by NAC, indicating that NAC reduced the production of EPS, in most bacteria tested, even at concentrations at which growth was not affected. Altogether, the presence of NAC changes the texture of the biofilm formed and makes NAC an interesting candidate for use as a general inhibitor of formation of bacterial biofilms on stainless steel surfaces.

Identification of lactobacilli with inhibitory effect on biofilm formation by pathogenic bacteria on stainless steel surfaces

2014 ◽  
Vol 191 ◽  
pp. 116-124 ◽  
Author(s):  
Fatma Ait Ouali ◽  
Imad Al Kassaa ◽  
Benoit Cudennec ◽  
Marwan Abdallah ◽  
Farida Bendali ◽  
...  
Keyword(s):  
Stainless Steel ◽  
Biofilm Formation ◽  
Pathogenic Bacteria ◽  
Inhibitory Effect ◽  
Steel Surfaces

scholarly journals Fire Ant Venom Alkaloids Inhibit Biofilm Formation

Toxins ◽  
2019 ◽  
Vol 11 (7) ◽  
pp. 420 ◽  
Author(s):  
Danielle Bruno de Carvalho ◽  
Eduardo Gonçalves Paterson Fox ◽  
Diogo Gama dos Santos ◽  
Joab Sampaio de Sousa ◽  
Denise Maria Guimarães Freire ◽  
...  
Keyword(s):  
Stainless Steel ◽  
Bacterial Adhesion ◽  
Solenopsis Invicta ◽  
Biofilm Formation ◽  
Food Industry ◽  
Fire Ant ◽  
Ant Venom ◽  
Steel Surfaces ◽  
Adhesion Inhibition ◽  
Medical Health

Biofilm formation on exposed surfaces is a serious issue for the food industry and medical health facilities. There are many proposed strategies to delay, reduce, or even eliminate biofilm formation on surfaces. The present study focuses on the applicability of fire ant venom alkaloids (aka ‘solenopsins’, from Solenopsis invicta) tested on polystyrene and stainless steel surfaces relative to the adhesion and biofilm-formation by the bacterium Pseudomonas fluorescens. Conditioning with solenopsins demonstrates significant reduction of bacterial adhesion. Inhibition rates were 62.7% on polystyrene and 59.0% on stainless steel surfaces. In addition, solenopsins drastically reduced cell populations already growing on conditioned surfaces. Contrary to assumptions by previous authors, solenopsins tested negative for amphipathic properties, thus understanding the mechanisms behind the observed effects still relies on further investigation.

Inhibitory Activity ofPaenibacillus polymyxaon the Biofilm Formation ofCronobacterspp. on Stainless Steel Surfaces

2013 ◽  
Vol 78 (7) ◽  
pp. M1036-M1040 ◽  
Author(s):  
Soonwook Yang ◽  
Seonhwa Kim ◽  
Jee-Hoon Ryu ◽  
Hoikyung Kim
Keyword(s):  
Stainless Steel ◽  
Inhibitory Activity ◽  
Biofilm Formation ◽  
Steel Surfaces

scholarly journals Evaluation of modified stainless steel surfaces targeted to reduce biofilm formation by common milk sporeformers

2016 ◽  
Vol 99 (12) ◽  
pp. 9502-9513 ◽  
Author(s):  
Shivali Jindal ◽  
Sanjeev Anand ◽  
Kang Huang ◽  
Julie Goddard ◽  
Lloyd Metzger ◽  
...  
Keyword(s):  
Stainless Steel ◽  
Biofilm Formation ◽  
Steel Surfaces
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