scholarly journals Biological Pathotyping of Newcastle Disease Viruses in Sudan 2008–2013

2014 ◽  
Vol 2014 ◽  
pp. 1-4 ◽  
Author(s):  
Egbal Sidahmed Abdelrahim Bilal ◽  
Iman Mohammed Elnasri ◽  
Aymen Mohamed Alhassan ◽  
Khalda Abdelaziz Khalifa ◽  
Jedddha Ibrahim Elhag ◽  
...  

The biological properties and pathogenicity of seven Newcastle disease virus field isolates were studied. These isolates were recovered from different outbreaks in Sudan (5 from chickens and 2 from pigeons) during 2008–2013. Based on intracerebral pathogenicity index, four NDV isolates were characterized as velogenic (their ICPI ranged 2.0–1.6) and three isolates were characterized as mesogenic (ICPI ranged 1.2–1.3). The mean death time for all isolates ranged from 54 to 76.8 hours. The elution time of the viruses from chicken erythrocytes and the ability to haemagglutinate mammalian red blood cells differed considerably in their reactions.

1948 ◽  
Vol 88 (2) ◽  
pp. 233-240 ◽  
Author(s):  
F. B. Bang

The application of the 50 per cent embryo mortality to a study of the virus of Newcastle is described. It has been evaluated by a series of duplicate titrations of the same sample of virus. In seven such titrations the largest difference between the two was 10–0.4. It is therefore believed that a difference of 0.6 log is probably significant and of 1.0 log almost certainly significant. This would mean that we can almost certainly detect a loss of 90 per cent of activity. Neither temperature of incubation nor route of inoculation in the test embryos had consistent effect on the measurement of virus activity. The effect of increasing age of the incubated embryo, from 10 days up to 16 days, is slight and inconsistent. The addition of chicken red blood cells to a dilution of virus may lower the titer of the preparation, but the change is not sufficient to be of importance in the routine handling of the virus.


1970 ◽  
Vol 2 (2) ◽  
pp. 113-116
Author(s):  
MA Amin ◽  
MM Amin ◽  
MSR Khan ◽  
KA Choudhury ◽  
MNA Siddiky ◽  
...  

Two isolates of Newcastle disease virus from two different caged birds, one from a parrot and another from a kakatoa were characterized during the period from July 2001 to October 2002. In HA tests both the isolates haemagglutinated chicken and guineapig erythrocytes but the parrot isolate was found refractory to bovine and equine erythrocytes and the kakatoa isolate to bovine erythrocytes. In elution tests, the parrot isolate was found to be a rapid eluter and the kakatoa isolate as slow eluter. Both the isolates were found to be heat-unstable in relation to haemagglutinating activity and embryo-infectivity. The mean death time (MDT) of the parrot and kakatoa isolates were 57.6 hours and 117.6 hours and the intracerebral pathogenicity indices (ICPl) were 1.58 and 0.45 respectively. So, the parrot isolate was found as the velogenic strain and the kakatoa isolate as the lentogenic strain of Newcastle disease virus.Key words: Caged bird; NDV; mean death time; ICPI; haemagglutination test. elution testdoi: 10.3329/bjvm.v2i2.2541Bangl. J. Vet. Med. (2004). 2 (2): 113-116


2014 ◽  
Vol 2014 ◽  
pp. 1-2
Author(s):  
Egbal Sidahmed Abdelrahim ◽  
Jedda Elhag

Two diseased red-headed lovebirds were presented for diagnosis to the Department of Avian Diseases and Diagnosis,Veterinary Research Institute, aged 37 days and 4 years. The symptoms were dyspnea, cyanosis of the comb, diarrhea, and fever. Postmortem lesions included pale liver and bloody enteritis. Newcastle disease virus was isolated from lungs, trachea, and intestines following inoculation in the allantoic cavity of 10-day-old fertile eggs; the NDV was identified by the means of HA&HI tests using specific NDV antisera (Lasota strain). The isolate agglutinated equine RBCs but failed to agglutinate sheep and bovine RBCs. The pathogenicity of the NDV isolate was studied, the mean death time was 96 hours, and the intracerebral pathogenicity index (ICPI) value was 0.9, indicating the isolate of lentogenic type.


1966 ◽  
Vol 21 (3) ◽  
pp. 254-260 ◽  
Author(s):  
R. Drzeniek ◽  
M. S. Saber ◽  
R. Rott

After erythrocytes had been treated with NDV, antigens of the Forssman and mononucleosis type could be demonstrated on their surface. The Forssman antigen was found to be either transfered by the virus to the surface of the erythrocytes or unmasked by splitting off sialic acid from chicken red cells. The mononucleosis antigen could only be transfered to the erythrocytes by NDV. Both antigens demonstrable in NDV, proved to be parts of the normal, host-specific component of the virus. The mononucleosis antigen of NDV was similar to that in bovine erythrocytes, but different from the one in sheep red blood cells.


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