scholarly journals Effects of the Combined PDL/Nd:YAG Laser on Surgical Scars: Vascularity and Collagen Changes Evaluated byIn VivoConfocal Microscopy

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Krisztina Vas ◽  
Magdolna Gaál ◽  
Erika Varga ◽  
Réka Kovács ◽  
Balázs Bende ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Laser Treatment ◽  
Clinical Assessment ◽  
Nd:Yag Laser ◽  
The Other ◽  
Long Term Effects ◽  
Short Term ◽  
Short Term Effects

The aim of this study was to investigate the efficacy of the sequential combined 585 nm PDL and the 1064 nm neodymium:yttrium-aluminium-garnet laser (PDL/Nd:YAG) in the treatment of surgical scars and to evaluate the short-term effects byin vivoconfocal microscopy (RCM) and the long-term effects by clinical assessment of the scars. Twenty-five patients were enrolled with 39 postoperative linear scars; each scar was divided into two fields. One half was treated with the combined PDL/Nd:YAG laser, whereas the other half remained untreated. Each scar was treated three times at monthly intervals. Scars were evaluated by an independent examiner, using the Vancouver Scar Scale. The combined PDL/Nd:YAG laser significantly improved the appearance of the scars. In order to study the short-term effects of combined laser treatment, six additional patients were enrolled with 7 postoperative linear scars. One half of scars was treated once with the combined PDL/Nd:YAG laser. One week after this laser treatment, both the treated and the nontreated parts of the scars were examined by dermoscopy and RCM. The dermoscopic pictures revealed improvements even in treated areas. In conclusion, the combined PDL/Nd:YAG laser was found to be effective in improving the quality and appearance of the surgical scars.

scholarly journals Effects of Long-Term Antiglaucoma Eye Drops on Conjunctival Structures: An In Vivo Confocal Microscopy Study

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Wenqing Zhu ◽  
Xiangmei Kong ◽  
Jianjiang Xu ◽  
Xinghuai Sun
Keyword(s):  
Confocal Microscopy ◽  
Cell Density ◽  
Laser Scanning ◽  
Open Angle Glaucoma ◽  
The Other ◽  
Control Group ◽  
Eye Drops ◽  
Long Term Effects ◽  
Ocular Surface Disease Index

Purpose. The study was aimed at comparing the long-term effects of different antiglaucoma eye drops on conjunctival structures using laser scanning confocal microscopy.Methods. Eighty patients diagnosed with primary open-angle glaucoma and twenty healthy volunteers were included in this study. The participants were divided into 5 groups according to the different medications. The lachrymal film break-up time, Schirmer’s I test, and Ocular Surface Disease Index Questionnaire were performed in all subjects. The confocal microscopy was used to observe the basal epithelial cell density (ECD), goblet cell density (GCD), dendritic cell density (DCD), and subepithelial collagen fiber diameter (SFD).Results. Statistically significant differences were found among the control group and the antiglaucoma therapy groups in the values of three clinical data (P<0.05). The GCD, DCD, and SFD showed significant differences in all glaucoma groups when compared to the control (P<0.001). Moreover, the prostaglandin group differed from the other antiglaucoma therapy groups in the GCD and SFD (P<0.05).Conclusions. Our study confirmed the significant differences in the conjunctival structures based on the effects of antiglaucoma medications. Less pronounced changes were found in the patients treated with prostaglandin analogue than in the other kinds of antiglaucoma therapies.

scholarly journals Short- and Long-Term Effects of Nonconsciously Processed Ambient Scents in a Servicescape: Findings From Two Field Experiments

2019 ◽  
Vol 22 (4) ◽  
pp. 440-455 ◽  
Author(s):  
Anna Girard ◽  
Marcel Lichters ◽  
Marko Sarstedt ◽  
Dipayan Biswas
Keyword(s):  
Large Scale ◽  
Field Experiments ◽  
Field Studies ◽  
Long Term Effects ◽  
Short Term ◽  
Railway Company ◽  
Short And Long Term ◽  
Ambient Scents ◽  
Short Term Effects

Ambient scents are being increasingly used in different service environments. While there is emerging research on the effects of scents, almost nothing is known about the long-term effects of consumers’ repeated exposure to ambient scents in a service environment as prior studies on ambient scents have been lab or field studies examining short-term effects of scent exposure only. Addressing this limitation, we examine the short- and long-term effects of ambient scents. Specifically, we present a conceptual framework for the short- and long-term effects of nonconsciously processed ambient scent in olfactory-rich servicescapes. We empirically test this framework with the help of two large-scale field experiments, conducted in collaboration with a major German railway company, in which consumers were exposed to a pleasant, nonconsciously processed scent. The first experiment demonstrates ambient scent’s positive short-term effects on consumers’ service perceptions. The second experiment—a longitudinal study conducted over a 4-month period—examines scent’s long-term effects on consumers’ reactions and demonstrates that the effects persist even when the scent has been removed from the servicescape.

Early Contact and Maternal-Infant Bonding: One Decade Later

PEDIATRICS ◽  
1982 ◽  
Vol 70 (5) ◽  
pp. 763-768
Author(s):  
Michael E. Lamb
Keyword(s):  
Child Development ◽  
Maternal Behavior ◽  
Present Review ◽  
Long Term Effects ◽  
Short Term ◽  
Maternal Bonding ◽  
Skin Contact ◽  
Skin To Skin ◽  
Short Term Effects

In the last decade, there has been considerable speculation concerning the importance of early skin-to-skin contact between parturient mothers and neonates. This contact is viewed as crucial to the occurrence of maternal bonding, which is seen as a precursor of optimal maternal behavior and thus as a necessary antecedent of optimal child development. In the present review, these conclusions are shown to have been based on equivocal findings obtained in methodologically impoverished studies. Although early contact may have modest but beneficial short-term effects in some circumstances, no positive long-term effects have been demonstrated.

Short-term effects of dopamine on photoreceptors, luminosity- and chromaticity-horizontal cells in the turtle retina

1995 ◽  
Vol 12 (3) ◽  
pp. 403-412 ◽  
Author(s):  
Josef Ammermüller ◽  
Reto Weiler ◽  
Ido Perlman
Keyword(s):  
Receptive Field ◽  
Time Course ◽  
Direct Action ◽  
Horizontal Cells ◽  
Field Size ◽  
Long Term Effects ◽  
Short Term ◽  
Short Term Effects

AbstractThe effects of dopamine on luminosity-type horizontal cells have been documented in different vertebrate retinas, both in vivo and in vitro. Some of these effects may reflect direct action of dopamine onto these cells, but indirect effects mediated by presynaptic neurons cannot be ruled out. Furthermore, direct effects of dopamine on horizontal cells may affect other, postsynaptic neurons in the outer plexiform layer. To test these possibilities, we studied the effects of dopamine on photoreceptors and all types of horizontal cells in the turtle (Pseudemys scripta elegans) retina. Receptive-field properties, responsiveness to light, and time course of light responses were monitored with intracellular recordings. Dopamine at a concentration of 40 μM exerted effects with two different time courses. “Short-term” effects were fully developed after 3 min of dopamine application and reversed within 30 min of washout of the drug. “Long-term” effects were fully developed after about 7–10 min and could not be washed out during the course of our experiments. Only the “short-term” effects were studied in detail in this paper. These were expressed in a reduction of the receptive-field size of all types of horizontal cells studied; L1 and L2 luminosity types as well as Red/Green and Yellow/Blue chromaticity types. The L1 horizontal cells did not exhibit signs of reduced responsiveness to light under dopamine, while in the L2 cells and the two types of chromaticity cells responsiveness decreased. None of the rods, long-wavelength-sensitive, or medium-wavelength-sensitive cones exhibited any apparent reduction in their receptive-field sizes or responsiveness to light. The present results suggest that the “short-term” effects of dopamine are not mediated by photoreceptors and are probably due to direct action of dopamine on horizontal cells.

scholarly journals Experiment in vivo: How COVID-19 Lifestyle Modifications Affect Migraine

2021 ◽  
Vol 12 ◽  
Author(s):  
Vesselina Grozeva ◽  
Ane Mínguez-Olaondo ◽  
Marta Vila-Pueyo
Keyword(s):  
Dietary Habits ◽  
Lifestyle Modification ◽  
Lifestyle Changes ◽  
Long Term Effects ◽  
Lifestyle Modifications ◽  
Short Term ◽  
Beneficial Effects ◽  
Compliance To Treatment

Introduction: The coronavirus disease 2019 (COVID-19) pandemic represents a unified lifestyle modification model, which was developed by the globally applied measures. The lockdowns designed the perfect study settings for observing the interaction between migraine and the adopted changes in lifestyle. An experiment in vivo took place unexpectedly to determine how the lockdown lifestyle modifications can influence migraine.Subsection 1: Overall lifestyle modifications during the pandemic: People stay home, and outdoor activities and public contacts are restricted. Sleep is disturbed. Media exposure and prolonged screen use are increased. Working conditions change. In-person consultations and therapies are canceled. The beneficial effects of short-term stress, together with the harmful effects of chronic stress, were observed during the pandemic.Subsection 2: Short-term effects: Substantial lifestyle changes happened, and knowing how vulnerable migraine patients are, one could hypothesize that this would have resulted in severe worsening of headache. Surprisingly, even though the impacts of changing social conditions were significant, some patients (including children) experienced a reduction in their migraine during the first lockdown.Subsection 3: Long-term effects: Unfortunately, headache frequency returned to the basal state during the second pandemic wave. The risk factors that could have led to this worsening are the long-term disruption of sleep and dietary habits, stress, anxiety, depression, non-compliance to treatment, and working during the pandemic.Discussion: Sudden short-term lifestyle changes taking migraine patients out of their usual routine may be beneficial for headache management. It is not necessary to have a natural disaster in place for a drastic lifestyle modification with 6–8-week duration, if we know that this will improve migraine.

scholarly journals Long-Term Deleterious Effects of Short-term Hyperoxia on Cancer Progression—Is Brain-Derived Neurotrophic Factor an Important Mediator? An Experimental Study

Cancers ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 688
Author(s):  
Adrian Tiron ◽  
Irina Ristescu ◽  
Paula A. Postu ◽  
Crina E. Tiron ◽  
Florin Zugun-Eloae ◽  
...  
Keyword(s):  
Cancer Progression ◽  
Neurotrophic Factor ◽  
Brain Derived Neurotrophic Factor ◽  
Long Term Effects ◽  
Short Term ◽  
Bdnf Expression ◽  
In Vivo Models

Perioperative factors promoting cancer recurrence and metastasis are under scrutiny. While oxygen toxicity is documented in several acute circumstances, its implication in tumor evolution is poorly understood. We investigated hyperoxia long-term effects on cancer progression and some underlying mechanisms using both in vitro and in vivo models of triple negative breast cancer (TNBC). We hypothesized that high oxygen exposure, even of short duration, may have long-term effects on cancer growth. Considering that hyperoxic exposure results in reactive oxygen species (ROS) formation, increased oxidative stress and increased Brain-Derived Neurotrophic Factor (BDNF) expression, BDNF may mediate hyperoxia effects offering cancer cells a survival advantage by increased angiogenesis and epithelial mesenchymal transition (EMT). Human breast epithelial MCF10A, human MDA-MB-231 and murine 4T1 TNBC were investigated in 2D in vitro system. Cells were exposed to normoxia or hyperoxia (40%, 60%, 80% O2) for 6 h. We evaluated ROS levels, cell viability and the expression of BDNF, HIF-1α, VEGF-R2, Vimentin and E-Cadherin by immunofluorescence. The in vivo model consisted of 4T1 inoculation in Balb/c mice and tumor resection 2 weeks after and 6 h exposure to normoxia or hyperoxia (40%, 80% O2). We measured lung metastases and the same molecular markers, immediately and 4 weeks after surgery. The in vitro study showed that short-term hyperoxia exposure (80% O2) of TNBC cells increases ROS, increases BDNF expression and that promotes EMT and angiogenesis. The in vivo data indicates that perioperative hyperoxia enhances metastatic disease and this effect could be BDNF mediated.

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