scholarly journals Composition, Repellent, and Insecticidal Activities of Two South American Plants against the Stored Grain Pests Tribolium castaneum and Tribolium confusum (Coleoptera: Tenebrionidae)

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Verónica Benzi ◽  
Natalia Stefanazzi ◽  
Ana Paula Murray ◽  
Jorge O. Werdin González ◽  
Adriana Ferrero

As part of a screening program to evaluate the biological activity of indigenous plants, we report the composition and the bioactivity of essential oils (EOs) extracted from Té de Burro Aloysia polystachya [(Griseb.) Moldenke] and Lemon Verbena Aloysia citriodora [Palau] against two of the most widespread secondary pests of stored products, the red flour beetle Tribolium castaneum [Herbst] and the confused flour beetle Tribolium confusum [Jacqueline du Val]. Analysis by gas chromatography-mass spectrometry of the EOs led the identification of their major constituents and their relative proportions. EO of A. citriodora showed the highest repellent activity against both beetles (>70%). On the other hand, both plants showed fumigant toxicity only against T. confusum, without significant differences between them (LC50 values of 5.92 and 5.53 mg/L air for A. polystachya and A. citriodora, resp.). For contact toxicity (topical applications) the EO of A. polystachya was more effective (LD50 = 7.35 μg/insect) than the EO of A. citriodora (LD50 = 13.8 μg/insect) only against T. castaneum. On the other hand, T. confusum was not susceptible by contact to any of these EOs. These results provide important tools for the development of an Integrated Pest Management (IPM) program.

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Abbas Khani ◽  
Tahere Rahdari

The biological activity of essential oil extracted from coriander, Coriandrum sativum L. (Apiaceae), seeds against adults of Tribolium confusum Duval (Coleoptera: Tenebrionidae) and Callosobruchus maculatus F. (Coleoptera: Bruchidae) was investigated in a series of laboratory experiments. Fumigant toxicity was assessed at 27±1°C and 65±5% R.H., in dark condition. Dry seeds of the plant were subject to hydrodistillation using a Clevenger-type apparatus. The composition of essential oil was analyzed by gas chromatography mass spectrometry. The predominant components in the oil were linalool (57.57%) and geranyl acetate (15.09%). The mortality of 1–7-day-old adults of the insect pests increased with concentration from 43 to 357 μL/L air and with exposure time from 3 to 24 h. In the probit analysis, LC50 values (lethal concentration for 50% mortality) showed that C. maculatus (LC50 = 1.34 μL/L air) was more susceptible than T. confusum (LC50 = 318.02 μL/L air) to seed essential oil of this plant. The essential oil of C. sativum can play an important role in stored grain protection and reduce the risks associated with the use of synthetic insecticides.


2013 ◽  
Vol 68 (1-2) ◽  
pp. 13-18 ◽  
Author(s):  
Zhi Long Liu ◽  
Guo Hua Jiang ◽  
Ligang Zhou ◽  
Qi Zhi Liu

Water-distilled essential oil from the aerial parts of Dipsacus japonicus Miq. (Dipsacaceae) at the flowering stage was analysed by gas chromatography-mass spectrometry (GC-MS). Forty-six compounds, accounting for 96.76% of the total oil, were identified and the main compounds of the essential oil were linalool (11.78%), trans-geraniol (8.58%), 1,8-cineole (7.91%), β-caryophyllene (5.58%), α-terpineol (5.32%), β-selinene (5.15%), and spathulenol (5.04%). The essential oil of D. japonicus possessed contact toxicity against two grain storage insects, Sitophilus zeamais and Tribolium castaneum adults, with LD50 values of 18.32 μg/ adult and 13.45 μg/adult, respectively. The essential oil of D. japonicus also exhibited pronounced fumigant toxicity against S. zeamais (LC50 = 10.11 mg/l air) and T. castaneum adults (LC50 = 5.26 mg/l air). Of the three major compounds, 1,8-cineole exhibited stronger fumigant toxicity than the crude essential oil against S. zeamais and T. castaneum adults with LC50 values of 2.96 mg/l air and 4.86 mg/l air, respectively


1970 ◽  
Vol 14 ◽  
pp. 43-48 ◽  
Author(s):  
Mina Mondal

Contact and fumigant toxicity of the three essential oils, viz., cardamom (Elletaria cardamomum Maton), Cinnamon (Cinnamomum aromaticum Nees), and Clove (Syzygium aromaticum (L.) Merr. and Petry) were tested against the red flour beetle, Tribolium castaneum (Herbst) larvae and adults. Residual film bioassay was employed in Petri dish (5 cm dia.) for contact toxicity studies and 6cm × 1.8cm glass vials were used for testing fumigation actions. Three day old adults and 10- day old larvae were equally susceptible to the contact toxicity of cinnamon oil, with LD50 values of 0.074 and 0.196 mg cm-2 respectively. Cardamom oil provided higher toxicity to 14-day and 18- day old larvae having LD50 value of 0.10mg cm-2. In fumigation bioassay cinnamon oil provided the highest toxicity to adult and 10-, 14-, and 18-day old larvae, with LD50 values of 0.03, 0.05, 0.088 and 0.09 mg cm-3 respectively. Furthermore, 10-day old larvae were more tolerant than the adults to the contact toxicity of the essential oils, but 14- day old larvae had the same susceptibility as the adults. In contact and fumigation toxicity adults and all stages of larvae were more resistant to clove oil. Key words: Bioassay; cardamom; clove; toxicity; essential oil J. bio-sci. 14: 1-9, 2006


2011 ◽  
Vol 6 (6) ◽  
pp. 1934578X1100600 ◽  
Author(s):  
Lilian R. Descamps ◽  
Carolina Sánchez Chopa ◽  
Adriana A. Ferrero

Essential oils extracted from leaves and fruits of Schinus areira (Anacardiaceae) were tested for their repellent, toxic and feeding deterrent properties against Tribolium castaneum (Coleoptera: Tenebrionidae) larvae and adults. A topical application assay was employed for the contact toxicity study and filter paper impregnation for the fumigant assay. A treated diet was also used to evaluate the repellent activity and a flour disk bioassay for the feeding deterrent action and nutritional index alteration. The essential oil of the leaves contained mainly monoterpenoids, with α-phellandrene, 3-carene and camphene predominant, whereas that from the fruits contained mainly α-phellandrene, 3-carene and β-myrcene. The leaf essential oil showed repellent effects, whereas that from the fruit was an attractant. Both oils produced mortality against larvae in topical and fumigant bioassays, but fumigant toxicity was not found against adults. Moreover, both essential oils produced some alterations in nutritional index. These results show that the essential oils from S. areira could be applicable to the management of populations of Tribolium castaneum.


1964 ◽  
Vol 42 (6) ◽  
pp. 757-762
Author(s):  
J. Leblanc ◽  
J. L. Villeneuve ◽  
A. Lemonde

Aminoguanidine, a histaminase inhibitor, was found to increase fecundity of Tribolium confusum (Coleoptera) when added as a supplement to the basal diet in concentrations of 0.01 and 0.5%. On the other hand alpha-methyl dopa, a decarboxylase inhibitor, had no effect on larval development except at a concentration of 1% where it was shown to be highly toxic. No catechol amines were found in adults of this species, but histamine was always detected and reached higher levels in insects fed higher concentrations of aminoguanidine. These results suggest a possible role for histamine in insect reproduction.


2015 ◽  
Vol 78 (4) ◽  
pp. 772-777 ◽  
Author(s):  
XIN CHAO LIU ◽  
ZHI LONG LIU

Water-distilled essential oil from Illicium henryi (Illiciaceae) root bark was analyzed by gas chromatography–mass spectrometry. Thirty-four compounds, accounting for 97.86% of the total oil, were identified. The main components of the essential oil of I. henryi root bark were safrole (46.12%), myristicin (20.39%), and 1,8-cineole (6.17%), followed by α-cadinol (3.784%) and linalool (3.22%). The essential oil had higher levels of phenylpropanoids (66.89%) than of monoterpenoids (14.83%) and sesquiternoids (16.14%). Three constituents were isolated from the oil based on bioactivity fractionation. The essential oil possessed fumigant toxicity against booklice (Liposcelis bostrychophila), with a 50% lethal concentration (LC50) of 380.39 μg/liter of air, while the two isolated constituents myristicin and safrole had LC50s of 121.95 and 322.54 μg/liter, respectively. Another constituent, 1,8-cineole, showed weaker toxicity, with an LC50 of 1,120.43 μg/liter. The essential oil also exhibited contact toxicity against L. bostrychophila, with an LC50 of 96.83 μg/cm2. Myristicin (LC50, 18.74 μg/cm2) and safrole (LC50, 69.28 μg/cm2) exhibited stronger acute toxicity than 1,8-cineole (LC50, 1,049.41 μg/cm2) against the booklice. The results indicated that the essential oil and its constituent compounds have potential for development into natural insecticides for control of psocids in stored grains.


Author(s):  
Tahany G. M. Mohammed ◽  
M. E. H. Nasr

Nanotechnology has enormous potential for developing alternative pest control strategies and reducing the risk of insecticide molecules. The present study aimed to develop a stable nanoemulsion (NE) of eucalyptus oil (EO) by the spontaneous emulsification method and evaluate its insecticidal and repellent effect against Sitophilus oryzae (L.), Rhizopertha dominica (F.) and Tribolium-castaneum (Herbst.). The prepared nanoemulsion formulation having a small particle size 8.57 nm with polydispersity index (PDI) 0.28. The study of the stability and physicochemical properties showed that the prepared formulation had good physical stability without any change in the macroscopic parameters. In addition, results showed that theinsecticidal activity of the prepared NE was higher than the original EO against the three tested insect speciesand the mortality increased with increasing concentrations and extending of exposure time. The contact toxicity of NE film revealed that, adults of R. dominica was more susceptible to all treatments followed by S. oryzae adults, while adults of T. castaneum was the least susceptible one, however, adults of S. oryzae was more susceptible followed by T. castaneum adults while, adults of                    R. dominica was the least susceptible one according to the fumigant toxicity and repellency. Our results suggested that the prepared formulation may be used in an integrated pest managementprogramfor controlling stored grain insects.


2019 ◽  
Vol 47 (2) ◽  
pp. 333-342
Author(s):  
Abu Faiz Md Aslam ◽  
Sharmin Sultana ◽  
Sumita Rani Das ◽  
Abdul Jabber Howlader

Tribolium confusum and Tribolium castaneum (Coleoptera: Tenebrionidae) are two very confusing pest species while identification is done on the basis of morphology only. Such pests are discovered in stored grain as immature stages, which further complicates the identification process. Accurate identification of these pests is urgently required for integrated pest management. In this research, DNA barcoding was used to identify these pests accurately at any life stage. A 658 bp fragment of the mitochondrial cytochrome c oxidase subunit I (COI) gene was analyzed. DNA barcode dataset of T. confusum (GeneBank Acc. no. MK120453.1) and T. castaneum (Acc. no. MK411585.1) were constructed. The nucleotide composition reveals that average AT contents (59.9%) were higher than the GC contents (38.6%). Phylogenetic analysis by maximum likelihood method showed that both the species were originated from a common major clade. About 17.13% nucleotide differences were noted between the CO1 sequences by multiple sequence alignment. The interspecies nucleotide genetic distance (0.200) was calculated using Kimura 2 parameter. Haplotype analysis showed high genetic diversity (112 mutaional steps) among them. Bangladesh J. Zool. 47(2): 333-342, 2019


1985 ◽  
Vol 6 (2) ◽  
pp. 217-220 ◽  
Author(s):  
Harish Chander ◽  
S. M. Ahmed

AbstractThe efficacy of natural embelin isolated from berries of Embelia ribes Burm. against Tribolium castaneum Herbst is discussed. Embelin mixed with wheat samples at concentrations of 0.1875% and above exhibited high efficacy by bringing about adult mortality even after 8 months of storage. Productivity (progeny/adult-day) of the test insect was also reduced significantly in treatments as compared to controls at different intervals of storage. After 8 months of storage, significant reduction in F1, progeny at the lowest concentration was mainly due to larvicidal action of embelin. In the present study, embelin did not exhibit any chemosterilant action or contact toxicity to adult beetles. Germination of wheat treated with embelin even at the highest concentration of 0.750% was not impaired to any significant extent.


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