scholarly journals A Simple Microfluidic Chip Design for Fundamental Bioseparation

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Alan S. Chan ◽  
Michael K. Danquah ◽  
Dominic Agyei ◽  
Patrick G. Hartley ◽  
Yonggang Zhu
Keyword(s):  
Rhodamine B ◽  
Silica Surface ◽  
Packed Column ◽  
Retention Factor ◽  
Chromatographic System ◽  
Group Separation ◽  
Good Separation ◽  
Chip Design ◽  
Liquid Chromatographic ◽  
Fluorescein Molecule

A microchip pressure-driven liquid chromatographic system with a packed column has been designed and fabricated by using poly(dimethylsiloxane) (PDMS). The liquid chromatographic column was packed with mesoporous silica beads of Ia3d space group. Separation of dyes and biopolymers was carried out to verify the performance of the chip. A mixture of dyes (fluorescein and rhodamine B) and a biopolymer mixture (10 kDa Dextran and 66 kDa BSA) were separated and the fluorescence technique was employed to detect the movement of the molecules. Fluorescein molecule was a nonretained species and rhodamine B was attached onto silica surface when dye mixture in deionized water was injected into the microchannel. The retention times for dextran molecule and BSA molecule in biopolymer separation experiment were 45 s and 120 s, respectively. Retention factor was estimated to be 3.3 for dextran and 10.4 for BSA. The selectivity was 3.2 and resolution was 10.7. Good separation of dyes and biopolymers was achieved and the chip design was verified.

Liquid Chromatographic Method for Determination of Piperine in Piper nigrum (Black and White Pepper)

1988 ◽  
Vol 71 (1) ◽  
pp. 53-55 ◽  
Author(s):  
Kenneth M Weaver ◽  
Michael E Neale ◽  
Ann Laneville
Keyword(s):  
Chromatographic Method ◽  
Piper Nigrum ◽  
Chromatographic System ◽  
Reverse Phase ◽  
White Pepper ◽  
Black And White ◽  
Liquid Chromatographic Method ◽  
Phase Liquid ◽  
Liquid Chromatographic

Abstract A method was developed for the detection and quantitation of piperine in Piper nigrum. A reverse-phase liquid chromatographic system equipped with a C18 column with detection at 340 nm was used. A rapid 1 h acetone extraction followed by solvent dilution was used to avoid sample cleanup. The detection limit is 3 ng injected piperine, with 97.5-100.5% recovery of added piperine.

Extraction of Organic Acids by Ion-Pair Formation with Tri-n-Octylamine. Part V. Simultaneous Determination of Synthetic Dyes, Benzoic Acid, Sorbic Acid, and Saccharin in Soft Drinks and Lemonade Syrups

1984 ◽  
Vol 67 (5) ◽  
pp. 880-885 ◽  
Author(s):  
Marc L Puttemans ◽  
Louis Dryon ◽  
Désiré L Massart
Keyword(s):  
Benzoic Acid ◽  
Sorbic Acid ◽  
Sodium Perchlorate ◽  
Soft Drinks ◽  
Synthetic Dyes ◽  
Chromatographic System ◽  
Reverse Phase ◽  
Phase Liquid ◽  
Liquid Chromatographic

Abstract Synthetic dyes, benzoic acid, sorbic acid, and saccharin are extracted simultaneously from soft drinks with 0.01M tri-n-octylamine at pH = 5.5 and are back-extracted to an aqueous phase with 0.1M sodium perchlorate. The perchlorate solution is injected directly into the reverse phase liquid chromatographic system which permits the separation of all the substances investigated. Forty-six commercial samples of soft drinks and 11 lemonade syrups were analyzed. All samples conformed to the legal prescriptions.

Determination of Ardacin in Various Silage Feed Diets by a Rapid Liquid Chromatographic Assay

1995 ◽  
Vol 78 (1) ◽  
pp. 16-21
Author(s):  
Sylvia V B Fagan ◽  
Connie Gombatz ◽  
Hafez Abdel-Kader ◽  
Govind Menon
Keyword(s):  
Mobile Phase ◽  
Solid Phase ◽  
Chromatographic System ◽  
Method Of Calculation ◽  
Feed Formulation ◽  
Sample Extract ◽  
Liquid Chromatographic ◽  
Solid Phase Extract ◽  
Cyano Column

Abstract A method is presented for the detection and quantitation of Ardacin in silage feed diets by liquid chromatography, using a cyano column and an acetonitrile–methanol water mobile phase modified with trifluoroacetic acid. This method includes comprehensive procedures for extracting Ardacin from various silage feed formulations, cleaning up the extracted sample by using solid-phase extractions, and analyzing the eluted solid-phase extract with a suitable liquid chromatographic system. Ardacin was extracted from the silage feed formulations with 50% acetonitrile and 50% 0.1 M KOH. The extract was cleaned up with a wide-pore butyl solidphase extraction cartridge. The sample extract was chromatographed and quantified at 220 nm, using an external method of calculation. Recoveries of the medicated silage feed formulation ranged from 72.1 ± 1.7% to 109.1 ± 2.4%, depending on the sites and types of formulation analyzed.

scholarly journals Determination of Phenylalanine and Tyrosine by Liquid Chromatography/Mass Spectrometry

2003 ◽  
Vol 86 (4) ◽  
pp. 753-758 ◽  
Author(s):  
Samo Andrensek ◽  
Alenka Golc-Wondra ◽  
Mirko Prosek
Keyword(s):  
Food Samples ◽  
Psychomotor Retardation ◽  
Blood Levels ◽  
Liquid Chromatography Mass Spectrometry ◽  
Good Separation ◽  
Ionization Mode ◽  
Reliable Quantification ◽  
Liquid Chromatographic ◽  
Chromatographic Mass

Abstract Phenylketonuria is a common metabolic disorder disease. Those affected appear normal at birth, but without treatment they develop severe psychomotor retardation. Throughout life, they must control their blood levels of phenylalanine (Phe) and consume a diet containing adequate amounts of Phe and tyrosine (Tyr). We have developed a liquid chromatographic/mass spectrometric (LC/MS) method for the quantitative evaluation of Phe and Tyr in food samples. This method takes advantage of the good separation of LC and the selective and reliable quantification provided by MS in the electrospray ionization mode. The LC/MS method is very suitable for the determination of selected amino acids in various matrixes. It is sensitive to levels as low as about 0.30 ppm for Tyr and 0.70 ppm for Phe and robust. Nearly 100 nondietary food samples were analyzed by the developed method.

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