scholarly journals Validation of a Real Time PCR for Classical Swine Fever Diagnosis

2014 ◽  
Vol 2014 ◽  
pp. 1-4 ◽  
Author(s):  
Natanael Lamas Dias ◽  
Antônio Augusto Fonseca Júnior ◽  
Anapolino Macedo Oliveira ◽  
Érica Bravo Sales ◽  
Bruna Rios Coelho Alves ◽  
...  
Keyword(s):  
Diagnostic Tests ◽  
Animal Health ◽  
Classical Swine Fever ◽  
Viral Disease ◽  
Economic Losses ◽  
Pcr Assay ◽  
Free Zone ◽  
The World ◽  
World Organization ◽  
Different Tissues

The viral disease classical swine fever (CSF), caused by aPestivirus, is one of the major causes of economic losses for pig farming. The aim of this work was to validate a RT-qPCR using Taqman for detection of CSF in swine tissues. The parameters for the validation followed the specifications of the Manual of Diagnostic Tests and Vaccines for Terrestrial Animals of the World Organization for Animal Health (OIE) and the guide ABNT NBR ISO/IEC 17025:2005. The analysis of the 5′NTR region of CSF virus was performed in 145 samples from 29 infected pigs and in 240 samples from 80 pigs originated in the Brazilian CSF-free zone. The tissues tested were spleen, kidney, blood, tonsils, and lymph nodes. Sequencing of the positive samples for 5′NTR region was performed to evaluate the specificity of the RT-qPCR. Tests performed for the RT-qPCR validation demonstrated that the PCR assay was efficient in detecting RNA from CSF virus in all materials from different tissues of infected animals. Furthermore, RNA from CSF virus was not detected in samples of swine originated from the Brazilian CSF-free zone. Hence, it is concluded that RT-qPCR can be used as a complementary diagnostic for CSF.

scholarly journals Current State of Global African Swine Fever Vaccine Development under the Prevalence and Transmission of ASF in China

Vaccines ◽  
2020 ◽  
Vol 8 (3) ◽  
pp. 531
Author(s):  
Keke Wu ◽  
Jiameng Liu ◽  
Lianxiang Wang ◽  
Shuangqi Fan ◽  
Zhaoyao Li ◽  
...  
Keyword(s):  
Vaccine Development ◽  
African Swine Fever Virus ◽  
Animal Health ◽  
African Swine Fever ◽  
Economic Losses ◽  
Future Research ◽  
Chinese Government ◽  
Current State ◽  
The World ◽  
World Organization

African swine fever (ASF) is a highly lethal contagious disease of swine caused by African swine fever virus (ASFV). At present, it is listed as a notifiable disease reported to the World Organization for Animal Health (OIE) and a class one animal disease ruled by Chinese government. ASF has brought significant economic losses to the pig industry since its outbreak in China in August 2018. In this review, we recapitulated the epidemic situation of ASF in China as of July 2020 and analyzed the influencing factors during its transmission. Since the situation facing the prevention, control, and eradication of ASF in China is not optimistic, safe and effective vaccines are urgently needed. In light of the continuous development of ASF vaccines in the world, the current scenarios and evolving trends of ASF vaccines are emphatically analyzed in the latter part of the review. The latest research outcomes showed that attempts on ASF gene-deleted vaccines and virus-vectored vaccines have proven to provide complete homologous protection with promising efficacy. Moreover, gaps and future research directions of ASF vaccine are also discussed.

scholarly journals The epidemiological background of small ruminant lentivirus infection in goats from Romania

2020 ◽  
Vol 13 (7) ◽  
pp. 1344-1350
Author(s):  
Adrian-Valentin Potarniche ◽  
Constantin Gheorghe Cerbu ◽  
Michal Czopowicz ◽  
Olga Szalus-Jordanow ◽  
Jaroslaw Kaba ◽  
...  
Keyword(s):  
Statistical Data ◽  
Small Ruminant ◽  
Animal Health ◽  
Viral Disease ◽  
International Literature ◽  
Main Obstacle ◽  
Individual Level ◽  
The World ◽  
World Organization ◽  
Control And Eradication

Background and Aim: Caprine arthritis-encephalitis (CAE) is an economically significant viral disease of goats caused by a small ruminant lentivirus (SRLV) belonging to Retroviridae family. This study aimed to summarize current information on the epidemiological status of SRLVs infection in the population of goats from Romania and to point out the CAE incidence throughout the 2008-2018 periods. Materials and Methods: An exhaustive review of the papers published in the international literature concerning the epidemiological status of CAE in Romania was carried out using electronic databases, and available statistical data from the World Organization for Animal Health (OIE) regarding the incidence of the disease between 2008 and 2018 were analyzed. Results: The true individual-level seroprevalence of CAE was estimated in 13 of 42 counties (31%) and ranged from 0.4% to roughly 40%. One hundred eighty-two outbreaks from 14 counties (33%) were reported, with a peak in 2010. Conclusion: The findings sourcing in the literature are very scarce and show disagreement with the situation reported by the national veterinary authorities. Lack of SRLVs screening policies represents the main obstacle in limiting the spread of the disease. Romania's National Sanitary Veterinary and Food Safety Authority should implement a program for diagnosis and surveillance of the disease to build a straightforward epidemiological picture that represents a prerequisite of any control and eradication program.

scholarly journals Classical Swine Fever Virus Biology, Clinicopathology, Diagnosis, Vaccines and a Meta-Analysis of Prevalence: A Review from the Indian Perspective

Pathogens ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 500 ◽  
Author(s):  
Yashpal Singh Malik ◽  
Sudipta Bhat ◽  
O. R. Vinodh Kumar ◽  
Ajay Kumar Yadav ◽  
Shubhankar Sircar ◽  
...  
Keyword(s):  
Disease Incidence ◽  
Meta Analysis ◽  
Animal Health ◽  
Group Analysis ◽  
Central Zone ◽  
Classical Swine Fever ◽  
Viral Disease ◽  
Control Measures ◽  
Phylogenetic Groups ◽  
The World

Classical swine fever (CSF) is an economically significant, multi-systemic, highly contagious viral disease of swine world over. The disease is notifiable to the World Organization for Animal Health (OIE) due to its enormous consequences on porcine health and the pig industry. In India, the pig population is 9.06 million and contributes around 1.7% of the total livestock population. The pig industry is not well organized and is mostly concentrated in the eastern and northeastern states of the country (~40% of the country’s population). Since the first suspected CSF outbreak in India during 1944, a large number of outbreaks have been reported across the country, and CSF has acquired an endemic status. As of date, there is a scarcity of comprehensive information on CSF from India. Therefore, in this review, we undertook a systematic review to compile and evaluate the prevalence and genetic diversity of the CSF virus situation in the porcine population from India, targeting particular virus genes sequence analysis, published reports on prevalence, pathology, and updates on indigenous diagnostics and vaccines. The CSF virus (CSFV) is genetically diverse, and at least three phylogenetic groups are circulating throughout the world. In India, though genotype 1.1 predominates, recently published reports point toward increasing evidence of co-circulation of sub-genotype 2.2 followed by 2.1. Sequence identities and phylogenetic analysis of Indian CSFV reveal high genetic divergence among circulating strains. In the meta-analysis random-effects model, the estimated overall CSF prevalence was 35.4%, encompassing data from both antigen and antibody tests, and region-wise sub-group analysis indicated variable incidence from 25% in the southern to nearly 40% in the central zone, eastern, and northeastern regions. A country-wide immunization approach, along with other control measures, has been implemented to reduce the disease incidence and eliminate the virus in time to come.

scholarly journals Achievements and Challenges of Classical Swine Fever Eradication in Brazil

Viruses ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 1327
Author(s):  
Luís Guilherme de Oliveira ◽  
Igor Renan Honorato Gatto ◽  
Marina Lopes Mechler-Dreibi ◽  
Henrique M. S. Almeida ◽  
Karina Sonálio ◽  
...  
Keyword(s):  
Industrial Production ◽  
Surveillance System ◽  
Animal Health ◽  
Classical Swine Fever ◽  
Federal District ◽  
Surveillance Systems ◽  
Free Zone ◽  
The North ◽  
Passive Surveillance System ◽  
Recent Occurrence

Classical swine fever virus (CSFV) causes one of the most critical diseases in the porcine industry worldwide. In Brazil, the first description of the infection was reported in 1888, and the national recognition of the first free zone (FZ) occurred in 2001. Brazil has been recently recognized (2015–2016) by the World Organisation for Animal Health (OIE) with an FZ involving 15 states and the Federal District, corresponding to 95% of the industrial production of pigs in the country, and a non-free zone (NFZ), comprised by the North and Northeast regions of the country, with approximately 18% of the national pig herd and 5% of industrial production. This review aims to describe the history, the control and eradication actions, the recent occurrence of outbreaks in the NFZ, and the results obtained by the surveillance systems’ action in the FZ for CSF in Brazil since its creation. In the passive surveillance system, the notification of the suspect cases of classical swine fever (CSF) is mandatory while in the active surveillance system adopted in the FZ consists of serological monitoring of certified swine breeding farms (CSBFs), intensive pig farming (IPF), non-technified pig herds (NTPig), surveillance in slaughterhouses and monitoring the populations of wild pigs. In this region, the last outbreaks of the disease occurred in 1998, while in the NFZ, 28 outbreaks were detected from 2005 to 2017, with an apparent lethality rate of 93.96% (840/894). However, in 2018 and 2019, 68 new outbreaks were registered with an apparent lethality rate of 75.05% (1095/1459). Therefore, in 2019, the Brazil CSF-Free Strategic Plan was created to eradicate the infection from the country’s NFZ, since outbreaks in this region present a risk of reintroducing the disease FZ. Finally, differences in characteristics between the regions show factors that still need to be considered for the construction of a robust surveillance system in the NFZ and some improvements in the FZ. Thus, the control of CSF throughout the Brazilian territory requires strict sanitary guidelines, promoting animal health and, consequently, the national production chain’s competitiveness.

scholarly journals FMD EPIDEMIC PROCESS CHARACTERISTICS IN RUSSIA IN 2010 – FIRST QUARTER OF 2019

2019 ◽  
pp. 39-44
Author(s):  
V. P. Semakina ◽  
T. P. Akimova ◽  
A. K. Karaulov
Keyword(s):  
Russian Federation ◽  
Animal Health ◽  
Foot And Mouth Disease ◽  
Viral Disease ◽  
Morbidity Rate ◽  
Primorsky Krai ◽  
African Countries ◽  
Epidemic Process ◽  
The World ◽  
The Russian Federation

Foot-and-mouth disease is a highly contagious viral disease of cloven-hoofed livestock and wild animals. Europe, North America and Oceania have long been FMD free; at the same time, the virus is widely spread in Asian and African countries. In the period from 2010 to March 2019, FMD was notified to the World Organisation for Animal Health (OIE) by 89 countries of the world. Local FMD outbreaks were also reported in several regions of the Russian Federation throughout this period. The research was aimed at studying some characteristics of the FMD epidemic process in the Russian Federation. The undertaken epidemiological analysis covers the Russian Federation regions where FMD outbreaks were reported between 2010 and March 2019. During the period under consideration, FMD cases were reported in 9 regions of the Russian Federation, mainly in the settlements located in close proximity to the Russia-China and Russia-Mongolia international borders. Most of the outbreaks were caused by serotype O and A FMD viruses. In most cases, FMD was reported in cattle and pigs and, less frequently, in sheep and goats. The analysis of the FMD epidemic situation in the Russian Federation Subjects was performed through epidemic process assessment based on the following estimates: the proportion of infected settlements, epidemic, contagiousness and morbidity rates. The Zabaykalsky and Primorsky Krais have a lead in the number of infected settlements. The highest morbidity rate in pigs was recorded in the Primorsky Krai, in cattle – in the Amur Oblast. The epidemic rate was the highest in the Primorsky and Zabaykalsky Krais. The Primorsky Krai also accounted for the highest contagiousness rates in 2014 and 2019 when FMD occurred on several large pig farms.

scholarly journals Does the infection with endoparasites influence the effect of oral vaccination against classical swine fever in wild boar?

2015 ◽  
Vol 84 (3) ◽  
pp. 225-230
Author(s):  
Anna Ondrejková ◽  
Ondrej Kiš ◽  
Juraj Ciberej ◽  
Katarína Oberhauserová ◽  
Róbert Ondrejka ◽  
...  
Keyword(s):  
Wild Boar ◽  
Classical Swine Fever ◽  
Viral Disease ◽  
Economic Losses ◽  
Parasitic Infections ◽  
Oral Vaccination ◽  
Specific Antibodies ◽  
Antibody Titres ◽  
In The Wild ◽  
Direct Elisa

Classical swine fever is a highly contagious viral disease that affects domestic and wild suids and could cause important economic losses. It is the most dangerous infectious disease of the wild boar that can cause severe death in densely populated areas. The aim of the study was to determine the effect of endoparasites on the oral vaccination against classical swine fever in wild boar. The study compared classical swine fever antibody titres in wild boar treated and untreated with antiparasitics. Fourteen six-month-old wild boar piglets were tested via direct ELISA to detect specific antibodies in blood serum after vaccination. Before the vaccination, one group of piglets was administered antiparasitic therapy; the other group of animals remained untreated. Twenty-eight days post vaccination, piglets from the first group (free of parasites) showed significantly (P = 0.0015) higher concentrations of specific antibodies than the infected animals. Obtained results proved that parasitic infections substantially influence the efficacy of oral vaccination against classical swine fever and may support the ability of the virus to produce infectious diseases and its transmission in the wild boar population. For that reason, antiparasitic therapy of wild boar populations before their vaccination is highly recommended in order to increase the vaccine’s efficacy.

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