scholarly journals Genotypic Diversity and Virulence Traits ofStreptococcus mutansIsolated from Carious Dentin after Partial Caries Removal and Sealing

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Nailê Damé-Teixeira ◽  
Rodrigo Alex Arthur ◽  
Clarissa Cavalcanti Fatturi Parolo ◽  
Marisa Maltz
Keyword(s):  
Streptococcus Mutans ◽  
Genotypic Diversity ◽  
Morphological Type ◽  
Wilcoxon Test ◽  
Arbitrary Primer ◽  
Dentin Caries ◽  
Virulence Traits ◽  
Before And After ◽  
Caries Removal ◽  
Partial Caries Removal

The aim of this study was to compare the genotypic diversity and virulence traits ofStreptococcus mutansisolated from carious dentin before and after partial dentin caries removal (PDR) and sealing. Carious dentin samples were obtained three months before and after the PDR and cavity sealing. Up to seven isolates of each morphological type ofS. mutanswere selected and strain identity was confirmed using gtfB primer. Genotyping was performed by arbitrary primer-PCR (AP-PCR). Acidogenesis and acidurance of the genotypes were evaluated as virulence traits. A pairedt-test and a Wilcoxon test were used to compare the virulence of genotypes. A total of 48 representativeS. mutansisolates were genotyped (31 before and 17 after the sealing). At least one of the genotypes found before the sealing was also found on dentin after the sealing. The number of genotypes found before the sealing ranged from 2 to 3 and after the sealing from 1 to 2 genotypes. No difference was observed in the acidogenesis and acidurance between genotypes isolated before and after the sealing. In conclusion, genotypic diversity ofS. mutansdecreased after the PDR and sealing, but the virulence traits ofS. mutansremained unchangeable.

Genotypic diversity and virulence traits of Streptococcus mutans in caries-free and caries-active individuals

2004 ◽  
Vol 53 (11) ◽  
pp. 1175-1175 ◽  
Author(s):  
M H Napimoga ◽  
R U Kamiya ◽  
R T Rosa ◽  
E AR Rosa ◽  
J F Höfling ◽  
...  
Keyword(s):  
Streptococcus Mutans ◽  
Genotypic Diversity ◽  
Virulence Traits

scholarly journals The effect of polymer burs on microbiological reduction of carious dentin in deciduous teeth: a systematic review

2016 ◽  
Vol 4 (1) ◽  
pp. 17-24 ◽  
Author(s):  
Daniela Silva Barroso de Oliviera ◽  
Driely Barreiros ◽  
Léa Assed Bezerra da Silva ◽  
Raquel Assed Bezerra da Silva ◽  
Paulo Nelson-Filho ◽  
...  
Keyword(s):  
Systematic Review ◽  
Streptococcus Mutans ◽  
New Technology ◽  
Permanent Teeth ◽  
Deciduous Teeth ◽  
Before And After ◽  
Quantitative Systematic Review ◽  
Caries Removal ◽  
Microbiological Reduction ◽  
Microbiological Aspects

Background. Polymer bur is a new technology that proposes to conserve the dentin that is capable of remineralization. Aim: To conduct a quantitative systematic review to evaluate the effect of polymer burs on the reduction of Streptococcus mutans and Lactobacillus after dentin carious excavation in deciduous teeth. Methods and Material: Two reviewers performed the database to identify the relevant clinical papers. Only papers in English that compared polymer burs with other caries removal techniques with the microbiological aspects as an outcome were included. Papers that evaluated only permanent teeth were excluded. Risk of bias was also assessed. Individual study effect sizes were calculated using Cohen’s d formula for the comparisons of microorganism reduction before and after carious excavation. Results: The search resulted in 12 non-duplicated papers. After the revision, only 2 were selected. The quantitative evaluation demonstrated that polymer bur reduces microorganism levels in carious dentin. The larger effect size observed was for Streptococcus mutans in the polymer bur technique (r=0.84; d=3.12), followed by Lactobacillus in the carbide bur technique (r=0.83; d=3.03). Conclusion: Polymer burs promoted a significant reduction of microorganism levels, mainly Streptococcus mutans in carious dentin. 

Genotypic diversity and virulence traits of Streptococcus mutans in caries-free and caries-active individuals

2004 ◽  
Vol 53 (7) ◽  
pp. 697-703 ◽  
Author(s):  
Marcelo Henrique Napimoga ◽  
Regianne Umeko Kamiya ◽  
Rosimeire Takaki Rosa ◽  
Edvaldo AntonioR. Rosa ◽  
José Francisco Höfling ◽  
...  
Keyword(s):  
Streptococcus Mutans ◽  
Glass Surface ◽  
Genetic Relatedness ◽  
Clonal Diversity ◽  
Genotypic Diversity ◽  
Pcr Analysis ◽  
Enzyme Electrophoresis ◽  
Bacterial Cells ◽  
Virulence Traits ◽  
Significant Difference

The present study evaluated the relationship between clonal diversity and some virulence traits of Streptococcus mutans isolated from eight caries-free and eight caries-active subjects. A total of 155 S. mutans isolates from caries-free subjects and 144 isolates from caries-active subjects were obtained from samples of saliva, dental plaque and tongue surface and identified by PCR. The isolates were submitted to arbitrarily primed (AP)-PCR (OPA-2 and OPA-13) and multilocus enzyme electrophoresis (MLEE) to establish the genotypic diversity. Production of water-insoluble glucan (WIG) (monitored by SDS-PAGE), final pH of cultures and the ability of bacterial cells to adhere to smooth glass in the presence of sucrose were measured. High and comparable abilities of MLEE and AP-PCR were found to distinguish S. mutans genotypes, using Simpson's index of discrimination (0.971 and 0.968, respectively). The results showed a significant difference (P < 0.01) in the number of genotypes when caries-free and caries-active groups were compared by both fingerprinting methods used. Final pH (P = 0.32) and the percentage of adherence to a glass surface (P = 0.62) did not show differences between the two groups; however, the intensities of WIG bands from the caries-active group were greater than those from the caries-free group (P < 0.01). In addition, WIG was positively correlated with the ability of S. mutans to adhere to a glass surface (r = 0.34, P = 0.02) from caries-active subjects. These data showed that AP-PCR analysis and MLEE are both effective methods for assessing the genetic relatedness of S. mutans. Using these techniques, it was found that there is a larger number of genotypes of S. mutans with increased ability to synthesize WIG in caries-active individuals.

EFEKTIVITAS BOOKLET TERHADAP PENGETAHUAN DAN SIKAP PEMELIHARAAN KESEHATAN GIGI MULUT PADA IBU HAMIL

2019 ◽  
Vol 4 (1) ◽  
pp. 66
Author(s):  
Rahmaini Fitri
Keyword(s):  
Oral Health ◽  
Pregnant Women ◽  
The Body ◽  
Wilcoxon Test ◽  
Percentage Increase ◽  
Oral Diseases ◽  
Health Maintenance ◽  
Knowledge And Attitudes ◽  
Before And After ◽  
Knowledge And Attitude

Pregnancy causes physiological changes in the body and as well as in the oral cavity. Dental and oral diseases associated with pregnancy that is, gingivitis, periodontitis and pregnancy granuloma. Mouth dental disease during pregnancy is not only influenced by the pregnancy itself but rather the lack of knowledge about dental and oral health maintenance. Efforts to improve the knowledge of pregnant women about oral health is done by providing information, information necessary for health education media. In this case the media is created and used to improve the knowledge of pregnant women is the booklet. This study aimed to analyze the differences in knowledge and attitudes before and after maintenance booklet oral health in pregnant women. This study is a quasi experiment with one group pre  and post test design. The sample is the first trimester pregnant women who come to the health center in Sentosa Baru Medan as many as 34 people. Analysis of the data  forcompare the average difference in scores of knowledge and attitudes before and after the intervention used the Wilcoxon test. The results showed there is an increased knowledge and attitude maintenance of oral health in pregnant women after being given a booklet with a value of p < 0.001, a percentage increase of 30% knowledge and attitudes percentage of 37%. The conclusions of this study is increased knowledge and attitude maintenance of oral health in pregnant women after being given a booklet.

Pengaruh Self Help Group Terhadap Kemampuan Keluarga Dalam Merawat Klien Skizofrenia Di Poli Jiwa Puskesmas Kalitidu

2020 ◽  
Vol 10 (2) ◽  
Author(s):  
Yusuf Efendi ◽  
Errix Kristian Julianto
Keyword(s):  
Mental Disorders ◽  
Group Intervention ◽  
Family Members ◽  
Wilcoxon Test ◽  
Sign Test ◽  
Significance Level ◽  
Self Help ◽  
Before And After ◽  
Self Help Groups ◽  
Self Help Group

ABSTRAKDiera perkembangan jaman saat ini, beberapa keluarga dihadapkan dengan permasalahna tentang adanya angggota keluarga yeng mengaami gangguan jiwa, tak jarang keluarga tidak mengetahui bagaimana merawat angota keluarga dengan gangguan jiwa. Self help group pada keluarga dengan gangguan jiwa perlu dilakukan untuk membantu keluarga mengatasi permasalahannya yang diselesaikan bersama dalam kelompok. Manfaat yang didapatkan pada terapi ini adalah terdapatnya peningkatan pengetahuan keluarga tentang Skizofrenia. Peningkatan pengetahuan ini akan berdampak terhadap kemampuan keluarga dalam merawat klien Skizofrenia..Desain penelitian ini menggunakan desain pre eksperimental dengan rancangan one group pre-posttest design. Sampel pada penelitian ini adalah keluarga penderita Skizofrenia di PKU Jiwa Kalitidu yang berjumlah 32. . Data dikumpulkan menggunakan kuesioner kemudian dianalisis dengan menggunakan uji Wolcoxon sign dengan tingkat kemaknaan 0,05. Hasil penelitian menunjukkan bahwa kondisi responden sebelum dan sesudah dilakukan intervensi dengan self help group pada kemampuan merawat dengan  nilai uji wilcoxon sebesar 0,001 yang berarti ada pengaruh dari intervensi self help group dengan merawat keluarga dengan gangguan jiwa. Kata Kunci       : Self Help Group, Kemampuan Merawat, Skizofrenia   ABSTRACT. In the current era of development, some families are faced with problems about family members who suffer from mental disorders, often families do not know how to care for family members with mental disorders. Self help groups for families with mental disorders need to be done to help families overcome the problems that are solved together in a group. The benefit of this therapy is that there is an increase in family knowledge about Schizophrenia. This increase in knowledge will have an impact on the ability of families to care for Schizophrenia clients.The design of this study used a pre-experimental design with one group pre-posttest design. The sample in this study was the families of Schizophrenics in  Kalitidu public helath centre, amounting to 32.. Data were collected using a questionnaire and then analyzed using the Wolcoxon sign test with a significance level of 0.05.The results showed that the condition of the respondents before and after the intervention with self help group on the ability to care for Wilcoxon test value of 0.001, which means there is an influence of self help group intervention by caring for families with mental disorders. Keywords: Self Help Group, Caring Ability, Schizophrenia

scholarly journals An Evaluation on Deep Caries Removal Method and Management Performed by Undergraduate Dental Students: A Malaysia Experience

2020 ◽  
Author(s):  
Nurin Izyani Othman ◽  
Hanan Umaira Ismail ◽  
Norazlina Mohammad ◽  
Norzalina Ghazali ◽  
Muhammad Syafiq Alauddin
Keyword(s):  
Current Knowledge ◽  
Glass Ionomer Cement ◽  
Dental Students ◽  
Primary Dentition ◽  
Permanent Teeth ◽  
Management Approach ◽  
Removal Method ◽  
Significant Difference ◽  
Caries Removal ◽  
Partial Caries Removal

Abstract Objectives The aim of this study is to investigate the current knowledge and attitude of deep caries removal method among dental students in Malaysia. Materials and Methods A total of 303 students (n = 303) responded to the online questionnaire. The first part of questionnaire was to evaluate the demographic data of the respondents and focused on the technique and management approach used for deep caries lesion. The second part investigated the preferred treatment used for deep caries based on the designated clinical case, while the third part assessed the factors that affected the decision on deep caries management. Statistical Analysis Independent t-test was used to compare difference between the two groups. Results Seventy four percent of the students have the knowledge of the different methods of caries removal, while 25.8% were only familiar with complete caries removal. The preferred method for deep caries removal in permanent teeth was partial caries removal (53%). For primary dentition, 45.6% of the students prefer to perform pulpotomy as compared with other techniques. There was no significant difference in caries removal method for permanent teeth between undergraduate year of study (p > 0.05), which was partial caries removal at 52.7 and 53.5%, respectively. For primary dentition, the preferred caries removal method was pulpotomy for year 4 (39.8%) and year 5 (52%) students. The popular material to restore deep caries was resin composite (42%) followed by glass ionomer cement (23.3%). Conclusions This study showed that partial caries removal was the preferred method despite partial understanding on the identification of the clinical indicators of the technique.

Perbedaan Pengetahuan Remaja Putri Sebelum Dan Sesudah Diberikan Penyuluhan Tentang Fluor Albus Di SMP Negeri 3 Penebel

2019 ◽  
Vol 2 (1) ◽  
pp. 18-23
Author(s):  
Kadek Sri Ariyanti ◽  
Rini Winangsih ◽  
Desak Made Firsia Sastra Putri
Keyword(s):  
Reproductive Health ◽  
Adolescent Girls ◽  
Young Women ◽  
Random Sampling ◽  
Statistical Test ◽  
Wilcoxon Test ◽  
Average Score ◽  
Average Increase ◽  
Before And After ◽  
Test Result

Background and purpose: Adolescence is a period of transition from childhood to adulthood. The problems facing adolescents are often related to reproductive health, one of which is fluorine albus. Many teenagers do not care about the fluoride of albus despite the enormous impact on women's reproductive health. One reason is the lack of knowledge about fluorine albus. Based on KISARA data there are 90.91% of girls in Denpasar City do not know the cause of fluorine albus. The purpose of this study was to determine differences in knowledge of young women before and after being given counseling about fluor albus. Method: The research design used was pre-experimental with one group pretest posttest design using 70 respondents taken by stratified random sampling. The results showed that the average score of adolescent girls' knowledge about fluor albus before being given counseling was 11.56 and after counseling was 14.23. There was an average increase in knowledge of 2.67. Results: Statistical test result using wilcoxon test get value p = 0.000 so that hypothesis accepted. Conculusion: It can be concluded that there is difference of adolescent knowledge about fluorine albus before and after given counseling.

The Effect of Breastfeeding Calendar Training on Knowledge and Attitudes of Mothers in Exclusive Breastfeeding

2021 ◽  
Vol 10 (1) ◽  
pp. 80-87
Author(s):  
Ari Indra Susanti ◽  
Aminarista Aminarista ◽  
Neneng Martini ◽  
Nur Rahmah ◽  
Sri Astuti
Keyword(s):  
Exclusive Breastfeeding ◽  
Health Workers ◽  
Sampling Technique ◽  
Wilcoxon Test ◽  
Test Design ◽  
Knowledge And Attitudes ◽  
Lactating Mothers ◽  
Before And After ◽  
Post Test ◽  
Quasi Experimental

Background: Lactating mothers were successful in giving exclusive breastfeeding as much as 42% based on SDKI (Survei Demografi Kesehatan Indonesia) in 2012. This is depend on the support of husbands and families as well as health workers. Thus, the husband or family should remind and motivate mothers as well as monitor the breastfeeding activity every day for 6 months using breastfeeding calendar.Objectives: This study aimed to determine the increase in knowledge and attitudes of mothers in providing exclusive breastfeeding after the exclusive breastfeeding calendar training.Materials and Methods: The research design used a quasi-experimental one group pre-test post-test design. This research was conducted on mothers who had babies (age 0-12 months) in Pasawahan Village and Pasawahan Kidul Village, Pasawahan District, Purwakarta Regency in April to November 2018, with 96 respondents. The sampling technique was conducted by purposive sampling. Data were collected by giving questionnaires to respondents, before and after the breastfeeding calendar training were given. The Wilcoxon test was used for the analysis of the data in this study.Results: The results showed that there were differences in the knowledge of mothers before and after training on breastfeeding calendar (p <0,000) and there were differences in the attitudes of mothers before and after the training on breastfeeding calendar (p <0,000).Conclusions: There was an increase in knowledge and changes in the attitude of mothers towards exclusive breastfeeding after being given the breastfeeding Calendar training. Therefore, suggestions for health workers, especially midwives and nutrition workers, can use the breastfeeding calendar as an educational medium to increase husband and family support in exclusive breastfeeding.

HOLY QURAN RECITATION OF SURAH AL-MULK AND AL-HASYR ON DECREASING ANXIETY IN MEDICAL STUDENTS

2021 ◽  
Vol 2 (1) ◽  
pp. 7-14
Author(s):  
Kaima Ishmata ◽  
Indri Seta Septadina ◽  
Bintang Arryotantri
Keyword(s):  
Medical Students ◽  
Sampling Technique ◽  
Simple Random Sampling ◽  
Complementary Therapy ◽  
Wilcoxon Test ◽  
Primary Data ◽  
Beck Anxiety Inventory ◽  
Cross Sectional ◽  
Before And After ◽  
Quasi Experimental

Anxiety is a feeling of discomfort, worry about something unclear and accompanied by autonomic symptoms. Murottal Al-Quran therapy is a complementary therapy with the most widely used Al-Qur'an media. This study aims to analyze the effect of the murottal Al-Qur'an Surah Al-Mulk and Al-Hasyr on the anxiety of medical students of Universitas Sriwijaya. This research is a quasi-experimental study with a cross-sectional approach with one group pre-post test design which was conducted from July to December 2020 at homes through Zoom meeting application. Sampling using simple random sampling technique. Data in the form of primary data using the Beck Anxiety Inventory (BAI) questionnaire.Primary data were collected using the Beck Anxiety Inventory (BAI) questionnaire before and after receiving the Murottal Al-Qur'an Surah Al-Mulk and Al-Hasyr for 7 consecutive days. The Shapiro-Wilk normality test was performed on the data, then analyzed with Paired t-test or Wilcoxon test using SPSS device. The Wilcoxon test results showed significant results of murottal Al-Qur'an Surah Al-Mulk and Al-Hasyr on anxiety (p = 0.000) in medical students. There is an effect of murottal Al-Qur’an surah Al-Mulk and Al-Hasyr on anxiety among medical students of Universitas Sriwijaya.

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