scholarly journals Enhanced Photosynthesis and Carbon Metabolism Favor Arsenic Tolerance in Artemisia annua, a Medicinal Plant as Revealed by Homology-Based Proteomics

2014 ◽  
Vol 2014 ◽  
pp. 1-21 ◽  
Author(s):  
Rashmi Rai ◽  
Sarita Pandey ◽  
Alok Kumar Shrivastava ◽  
Shashi Pandey Rai
Keyword(s):  
Medicinal Plant ◽  
Artemisia Annua ◽  
Arsenic Tolerance ◽  
Transporter Protein ◽  
Differentially Expressed Protein ◽  
Rieske Proteins ◽  
Primary And Secondary Metabolism ◽  
As Stress ◽  
Novel Protein

This paper provides the first proteomic evidence of arsenic (As) tolerance and interactive regulatory network between primary and secondary metabolism in the medicinal plant, Artemisia annua. While chlorophyll fluorescence and photosynthetic rate depicted mild inhibition, there was a significant enhancement in PSI activity, whole chain, ATP, and NADPH contents in 100 μM As treatments compared to the control plants. However, a decrease in the above variables was recorded under 150 μM treatments. Proteomic decoding of the survival strategy of A. annua under As stress using 2-DE followed by MALDI-MS/MS revealed a total of 46 differentially expressed protein spots. In contrast to other plants where As inhibits photosynthesis, A. annua showed appreciable photosynthetic CO2 assimilation and allocation of carbon resources at 100 μM As concentration. While an increased accumulation of ATP synthase, ferredoxin-NADP(H) oxidoreductase, and FeS-rieske proteins supported the operation of cyclic electron transport, mdr ABC transporter protein and pcs gene might be involved in As detoxification. The most interesting observation was an increased accumulation of LEAFY like novel protein conceivably responsible for an early onset of flowering in A. annua under As stress. This study not only affirmed the role of energy metabolism proteins but also identified potential candidates responsible for As tolerance in plants.

Role of impurities in purification of artemisinin from Artemisia annua extracts

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
C Malwade ◽  
H Qu ◽  
BG Rong ◽  
LP Christensen
Keyword(s):  
Artemisia Annua

scholarly journals Early Consumption of Cannabinoids: From Adult Neurogenesis to Behavior

2021 ◽  
Vol 22 (14) ◽  
pp. 7450
Author(s):  
Citlalli Netzahualcoyotzi ◽  
Luis Miguel Rodríguez-Serrano ◽  
María Elena Chávez-Hernández ◽  
Mario Humberto Buenrostro-Jáuregui
Keyword(s):  
Young Adulthood ◽  
Adult Neurogenesis ◽  
Critical Period ◽  
Consumption Rate ◽  
Behavioral Outcomes ◽  
Endocannabinoid System ◽  
Illegal Drug ◽  
Adult Brain ◽  
As Stress

The endocannabinoid system (ECS) is a crucial modulatory system in which interest has been increasing, particularly regarding the regulation of behavior and neuroplasticity. The adolescent–young adulthood phase of development comprises a critical period in the maturation of the nervous system and the ECS. Neurogenesis occurs in discrete regions of the adult brain, and this process is linked to the modulation of some behaviors. Since marijuana (cannabis) is the most consumed illegal drug globally and the highest consumption rate is observed during adolescence, it is of particular importance to understand the effects of ECS modulation in these early stages of adulthood. Thus, in this article, we sought to summarize recent evidence demonstrating the role of the ECS and exogenous cannabinoid consumption in the adolescent–young adulthood period; elucidate the effects of exogenous cannabinoid consumption on adult neurogenesis; and describe some essential and adaptive behaviors, such as stress, anxiety, learning, and memory. The data summarized in this work highlight the relevance of maintaining balance in the endocannabinoid modulatory system in the early and adult stages of life. Any ECS disturbance may induce significant modifications in the genesis of new neurons and may consequently modify behavioral outcomes.

SATP (YaaH), a succinate–acetate transporter protein in Escherichia coli

2013 ◽  
Vol 454 (3) ◽  
pp. 585-595 ◽  
Author(s):  
Joana Sá-Pessoa ◽  
Sandra Paiva ◽  
David Ribas ◽  
Inês Jesus Silva ◽  
Sandra Cristina Viegas ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Acetic Acid ◽  
Succinic Acid ◽  
Critical Role ◽  
Amino Acid Residues ◽  
E Coli ◽  
Transporter Protein ◽  
Affinity Constants ◽  
In Trans

In the present paper we describe a new carboxylic acid transporter in Escherichia coli encoded by the gene yaaH. In contrast to what had been described for other YaaH family members, the E. coli transporter is highly specific for acetic acid (a monocarboxylate) and for succinic acid (a dicarboxylate), with affinity constants at pH 6.0 of 1.24±0.13 mM for acetic acid and 1.18±0.10 mM for succinic acid. In glucose-grown cells the ΔyaaH mutant is compromised for the uptake of both labelled acetic and succinic acids. YaaH, together with ActP, described previously as an acetate transporter, affect the use of acetic acid as sole carbon and energy source. Both genes have to be deleted simultaneously to abolish acetate transport. The uptake of acetate and succinate was restored when yaaH was expressed in trans in ΔyaaH ΔactP cells. We also demonstrate the critical role of YaaH amino acid residues Leu131 and Ala164 on the enhanced ability to transport lactate. Owing to its functional role in acetate and succinate uptake we propose its assignment as SatP: the Succinate–Acetate Transporter Protein.

scholarly journals The Transcription Factor Con7-1 Is a Master Regulator of Morphogenesis and Virulence in Fusarium oxysporum

2015 ◽  
Vol 28 (1) ◽  
pp. 55-68 ◽  
Author(s):  
Carmen Ruiz-Roldán ◽  
Yolanda Pareja-Jaime ◽  
José Antonio González-Reyes ◽  
M. Isabel G. Roncero
Keyword(s):  
Cell Wall ◽  
Fusarium Oxysporum ◽  
Gene Expression Analysis ◽  
Wall Structure ◽  
Targeted Deletion ◽  
Signal Perception ◽  
Cell Wall Biogenesis ◽  
Primary And Secondary Metabolism ◽  
Genes Encoding

Previous studies have demonstrated the essential role of morphogenetic regulation in Fusarium oxysporum pathogenesis, including processes such as cell-wall biogenesis, cell division, and differentiation of infection-like structures. We identified three F. oxysporum genes encoding predicted transcription factors showing significant identities to Magnaporthe oryzae Con7p, Con7-1, plus two identical copies of Con7-2. Targeted deletion of con7-1 produced nonpathogenic mutants with altered morphogenesis, including defects in cell wall structure, polar growth, hyphal branching, and conidiation. By contrast, simultaneous inactivation of both con7-2 copies caused no detectable defects in the resulting mutants. Comparative microarray-based gene expression analysis indicated that Con7-1 modulates the expression of a large number of genes involved in different biological functions, including host–pathogen interactions, morphogenesis and development, signal perception and transduction, transcriptional regulation, and primary and secondary metabolism. Taken together, our results point to Con7-1 as general regulator of morphogenesis and virulence in F. oxysporum.

TRiC-P5, a novel TCP1-related protein, is localized in the cytoplasm and in the nuclear matrix

1994 ◽  
Vol 107 (10) ◽  
pp. 2851-2859
Author(s):  
E.C. Joly ◽  
E. Tremblay ◽  
R.M. Tanguay ◽  
Y. Wu ◽  
V. Bibor-Hardy
Keyword(s):  
Nuclear Matrix ◽  
Cellular Localization ◽  
Cell Fractionation ◽  
Cellular Functions ◽  
Raji Cells ◽  
T Complex ◽  
Ring Complex ◽  
Filament Network ◽  
Novel Protein

We have recently reported the cloning of a novel protein, TRiC-P5, with significant homology with protein 1 of the t-complex (TCP1). In the present study, the cellular localization of TRiC-P5 in Raji cells has been determined using an antiserum raised against a 18.5 kDa fusion protein. Results from cell fractionation and immunoblot studies indicate that TRiC-P5 is mainly localized in the cytoplasm. In addition, a significant part of TRiC-P5 is also found in the nucleus where it is attached to the nuclear matrix, a complex filament network involved in essential cellular functions such as DNA replication, and RNA transcription and maturation. Immunofluorescence experiments using the anti-TRiC-P5 antibodies confirm these results. We also provide evidence that, in the cytoplasm, TRiC-P5 is part of a large protein complex, most probably the TCP1-ring complex (TRiC), a hetero-oligomeric ring complex that plays a role of molecular chaperone in the folding of actin and tubulin.

scholarly journals Effects of Happiness on Mental Health

2015 ◽  
Vol 2 (3) ◽  
Author(s):  
Dr. Gopal Chandra Mahakud ◽  
Ritika Yadav
Keyword(s):  
Mental Health ◽  
Mental Disorders ◽  
Social Situation ◽  
Psychological Problem ◽  
Health Conditions ◽  
Positive Attitudes ◽  
Psychic Disorders ◽  
As Stress ◽  
The Individual

The concept of mental health comprised to the health conditions of people without suffering any mental or psychological problem such as stress, depression, anxiety and other form of psychic disorders. In this regard it can be said that no one is free from and psychological, psycho-physical and psycho-social disorders from which we can derive that no one mentally healthy. But the concept of mental health defined free from the disorders those are prolonged and panic in nature. As the concept of mental health is subjective in nature, it varies from person to person. Besides free from the disorders, a person should pose some of the other positive characteristics to deal with the society effectively. Marry (1958) stated that, a person can be considered mentally healthy with the following characteristics such as (a) Positive attitudes toward himself/herself; (b) Realization of own potentialities through action; (c); Unification of in personality; (d) Degree of independence of social influences; (e) observations of the world around; and (f) Positive adapts to everyday life. Briefly, it can be said that positive mental health of the person make able to an individual to stand on his own two feet without making undue demands or impositions of others. In this regard the role of happiness in day to day life can make the individual more skilled to fight with different mental disorders. The present article is intended to find out the effects of happiness in day to day life in a social situation to deal with different mental disorders to make the individual mentally healthy and prosperous in life.

scholarly journals Crianças com incontinência urinária: impacto na convivência dos familiares

2018 ◽  
Vol 12 (7) ◽  
pp. 2061
Author(s):  
Ivanda Araújo Matias Issa de Oliveira ◽  
Cristiane Feitosa Salviano ◽  
Gisele Martins
Keyword(s):  
Urinary Incontinence ◽  
Family Members ◽  
Health Science ◽  
Related Family ◽  
Calidad De Vida ◽  
Family Routine ◽  
As Stress ◽  
The Impact

RESUMOObjetivo: Identificar fatores que impactam na convivência dos familiares de crianças com incontinência urinária. Método: Estudo bibliográfico, descritivo, tipo revisão integrativa, com busca de artigos no mês de setembro de 2017, nas bases de dados LILACS, BDENF, MEDLINE e CINAHL. Considerou-se o recorte temporal de janeiro de 2012 a dezembro de 2017, utilizando os Descritores em Ciência da Saúde (DeCS) controlados e não controlados no idioma inglês e português. Resultados: Foram incluídos 11 artigos, publicados entre 2012 e 2016, destacando-se três categorias: 1) O nível educacional dos pais como um fator de impacto; 2) O impacto na qualidade de vida dos familiares; e 3) As mudanças que impactam no cotidiano familiar. Conclusões: A incontinência urinária afeta a rotina familiar e pode provocar transtornos psicológicos como estresse, ansiedade e depressão nas crianças e em seus familiares. Houve escassez de produções que relacionassem a percepção do familiar ante a incontinência urinária diurna e fecal com o nível escolar dos pais. Evidencia-se o papel do enfermeiro que atua em uropediatria sobre a importância da compreensão da convivência familiar, a fim de contribuir com o delinear de orientações voltadas para a educação e compreensão das experiências vividas pelos cuidadores. Descritores: Incontinência Urinária; Família; Cuidadores; Crianças; Enurese; Incontinência Urinária por Estresse.ABSTRACTObjective: To identify factors that have an impact on the coexistence of family members of children with urinary incontinence. Method: Bibliographic, descriptive, integrative review type study with search of articles in September 2017, in LILACS, BDENF, MEDLIN, and CINAHL databases. We considered the temporal cut from January 2012 to December 2017, using controlled and uncontrolled Health Science Descriptors (DeCS) in English and Portuguese. Results: We included 11 articles, published between 2012 and 2016, highlighting three categories: 1) The educational level of parents as an impact factor; 2) The impact on the quality of life of family members; and 3) The changes that impact on daily family life. Conclusions: Urinary incontinence affects the family routine and can cause psychological disorders, such as stress, anxiety, and depression in children and their family members. There was a shortage of productions that related family members' perceptions of diurnal urinary incontinence and fecal incontinence to the parents' education level. The role of nurses working in pediatric urology was evident with respect to the importance of understanding family coexistence in order to contribute to the delineation of guidelines aimed at the education and understanding of caregivers' experiences. Descriptors: Urinary Incontinence; Family; Caregivers; Children; Enuresis; Urinary Incontinence Due to Stress.RESUMENObjetivo: Identificar factores que impactan en la convivencia de los familiares de niños con incontinencia urinaria. Método: Estudio bibliográfico, descriptivo, tipo revisión integradora, con búsqueda de artículos en el mes de septiembre de 2017, en las bases de datos LILACS, BDENF, MEDLINE y CINAHL. Fue considerado el recorte temporal de enero de 2012 a diciembre de 2017, utilizando los Descriptores en Ciencias de la Salud (DeCS) controlados y no controlados en idioma Inglés y portugués. Resultados: Se incluyeron 11 artículos publicados entre 2012 y 2016, destacándose tres categorías: 1) El nivel educativo de los padres como un factor de impacto; 2) El impacto en la calidad de vida de los familiares; y 3) Los cambios que impactan en el cotidiano familiar. Conclusiones: La incontinencia urinaria afecta la rutina familiar y puede provocar trastornos psicológicos como estrés, ansiedad y depresión en los niños y en sus familiares. Hubo escasez de producciones que relacionaran la percepción de los familiares ante la incontinencia urinaria diurna e incontinencia fecal con el nivel escolar de los padres. Se evidencia el papel del enfermero que actúa en urología pediátrica con respecto a la importancia de la comprensión de la convivencia familiar, a fin de contribuir con el delinear de orientaciones sobre la educación y comprensión de las experiencias vividas por los cuidadores. Descriptores: Incontinencia Urinaria; Familia; Cuidadores; Niños; Enuresis; Incontinencia Urinaria por Estrés.

Sign in / Sign up

Export Citation Format

Share Document