scholarly journals Tobacco Exposure by Various Modes May Alter Proinflammatory (IL-12) and Anti-Inflammatory (IL-10) Levels and Affects the Survival of Prostate Carcinoma Patients: An Explorative Study in North Indian Population

2014 ◽  
Vol 2014 ◽  
pp. 1-11 ◽  
Author(s):  
Shailendra Dwivedi ◽  
Apul Goel ◽  
Sanjay Khattri ◽  
Anil Mandhani ◽  
Praveen Sharma ◽  
...  
Keyword(s):  
Cancer Patients ◽  
Prostate Carcinoma ◽  
Interleukin 10 ◽  
Interleukin 12 ◽  
Tobacco Exposure ◽  
Statistical Tool ◽  
Cytokine Levels ◽  
Test Graph ◽  
Anti Inflammatory ◽  
Alcohol Users

Objective. Inflammation is an important hallmark of all cancers and net inflammatory response is determined by a delicate balance between pro- and anti-inflammatory cytokines, which may be affected by tobacco exposure, so the present study was designed to explore the effect of various modes of tobacco exposure on interleukin-12 (IL-12) and interleukin-10 (IL-10) inflammatory cytokine levels and survival in prostate carcinoma (PCa) patients.Methods. 285 cancer patients and equal controls with 94 BPH (benign prostatic hyperplasia) were recruited; baseline levels of serum IL-12 and IL-10 were measured and analyzed in various tobacco exposed groups by appropriate statistical tool. Five-year survivals of patients were analyzed by Log-rank (Mantel-Cox) test (graph pad version 5).Results. The expression of serum proinflammatory (IL-12) and anti-inflammatory (IL-10) cytokines was correlated with tobacco exposed group as smokers, chewers, and alcohol users have shown significantly higher levels (P<0.001) with significantly lower median survivals (27.1 months, standard error = 2.86, and 95% CI: 21.4–32.62); than nonusers. Stages III and IV of tobacco addicted patients have also shown significantly increased levels of IL-12 and IL-10.Conclusions. IL-12 and IL-10 seem to be affected by various modes of tobacco exposure and inflammation also affects median survival of cancer patients.

scholarly journals Controlling Gut Inflammation by Restoring Anti-Inflammatory Pathways in Inflammatory Bowel Disease

Cells ◽  
2019 ◽  
Vol 8 (5) ◽  
pp. 397 ◽  
Author(s):  
Paolo Giuffrida ◽  
Sara Cococcia ◽  
Mariangela Delliponti ◽  
Marco Vincenzo Lenti ◽  
Antonio Di Sabatino
Keyword(s):  
Inflammatory Bowel Disease ◽  
Side Effects ◽  
Bowel Disease ◽  
Transforming Growth Factor ◽  
Transforming Growth Factor Β ◽  
Interleukin 10 ◽  
Interleukin 12 ◽  
Inflammatory Pathways ◽  
Inflammatory Bowel ◽  
Anti Inflammatory

Inflammatory bowel disease (IBD) is caused by a dysregulated immune response against normal components of the intestinal microflora combined with defective functioning of anti-inflammatory pathways. Currently, all therapies approved for IBD manipulate the immune system by inhibiting pro-inflammatory mechanisms, such as tumor necrosis factor-α, gut-homing α4β7 integrin, interleukin-12/interleukin-23, and Janus kinases. However, some IBD patients are non-responders to these drugs, which are also associated with serious side effects. Thus, it has been hypothesized that therapies aimed at restoring anti-inflammatory signals, by exploiting the tolerogenic potential of cytokines (interleukin-10, transforming growth factor-β, granulocyte macrophage colony-stimulating factor), immune cells (regulatory T cells, tolerogenic dendritic cells), or mesenchymal stem cells, might offer promising results in terms of clinical efficacy with fewer side effects. In this review, we provide new insights into putative novel treatments aimed at restoring anti-inflammatory signaling pathways in IBD.

scholarly journals Involvement of Interleukin-10 in the Anti-Inflammatory Effect of Sanyinjiao (SP6) Acupuncture in a Mouse Model of Peritonitis

2011 ◽  
Vol 2011 ◽  
pp. 1-9 ◽  
Author(s):  
Morgana Duarte da Silva ◽  
Giselle Guginski ◽  
Maria Fernanda de Paula Werner ◽  
Cristiane Hatsuko Baggio ◽  
Rodrigo Marcon ◽  
...  
Keyword(s):  
Vascular Permeability ◽  
Interleukin 10 ◽  
Sham Acupuncture ◽  
Therapeutic Effects ◽  
Plasma Extravasation ◽  
Sterile Saline ◽  
Cell Counts ◽  
Cytokine Levels ◽  
Anti Inflammatory ◽  
Inflammatory Effect

In this study, we determined the anti-inflammatory effect of manual acupuncture at the Sanyinjiao or Spleen 6 (SP6) point on carrageenan-induced peritonitis in mice and investigated mechanisms that may underlie this effect. In the first set of experiments, male Swiss mice were allocated into five groups: the control (sterile saline), dexamethasone (DEXA), invasive sham-acupuncture (non-acupoint), SP6 acupuncture and carrageenan-treated groups. Ten minutes after needle retention or 30 min after DEXA treatment, mice received an intraperitoneal injection of carrageenan (750 μg/mouse). After 4 h, total leukocyte and differential cell counts (neutrophils and mononuclear), myeloperoxidase (MPO) activity, vascular permeability and cytokine levels were evaluated. In another set of experiments, adrenalectomized (ADX) mice were used to study the involvement of the adrenal gland on the therapeutic effects of acupuncture. Mice were allocated into two groups: the ADX and sham-operated animals (Sham ADX) that were subdivided into four subgroups each: the control (sterile saline), DEXA, SP6 acupuncture and carrageenan-treated groups. The SP6 and DEXA treatments inhibited the inflammatory cell infiltration, vascular permeability and MPO activity in carrageenan-injected mice. In addition, the SP6 treatment also increased interleukin (IL)-10 levels. In contrast, when the animals were adrenalectomized, the SP6 treatment failed to reduce total leukocyte and the plasma extravasation. In conclusion, this study clearly demonstrates the anti-inflammatory effect of SP6 acupuncture in a model of carrageenan-induced peritonitis. Our results demonstrated that SP6 acupuncture depends of the adrenal glands and increased IL-10 levels to produce its anti-inflammatory action.

Time course of pro- and anti-inflammatory cytokine levels in patients with burns—Prognostic value of interleukin-10

Burns ◽  
2010 ◽  
Vol 36 (4) ◽  
pp. 483-494 ◽  
Author(s):  
C. Csontos ◽  
V. Foldi ◽  
L. Pálinkas ◽  
L. Bogar ◽  
E. Röth ◽  
...  
Keyword(s):  
Prognostic Value ◽  
Inflammatory Cytokine ◽  
Time Course ◽  
Interleukin 10 ◽  
Cytokine Levels ◽  
Anti Inflammatory ◽  
Anti Inflammatory Cytokine

Influence of Polymorphisms of Pro-Inflammatory (IL-12, TNFa and LTa) and Anti-Inflammatory Factors (IL-10 and CTLA4) in Autoimmune Hemolytic Anemia.

Blood ◽  
2007 ◽  
Vol 110 (11) ◽  
pp. 3751-3751
Author(s):  
Maria S. Figueiredo ◽  
Leandro S. D’Abronzo ◽  
Melca M. Oliveira ◽  
Juliana L. Dreyfuss ◽  
Jose Orlando Bordin
Keyword(s):  
Hemolytic Anemia ◽  
Patient Group ◽  
Autoimmune Hemolytic Anemia ◽  
Interleukin 10 ◽  
Inflammatory Factors ◽  
Interleukin 12 ◽  
Control Group ◽  
Nucleotide Polymorphisms ◽  
Necrosis Factor Alpha ◽  
Anti Inflammatory

Abstract The Autoimmune Hemolytic Anemia (AIHA) is caused by the destruction of antibody-coated red blood cells, but mechanisms that initiate the production of autoantibodies remains unclear. It had been suggest that decreased production of Th1-type cytokines and production of autoantibodies in AIHA can be secondary to the imbalance between anti- and pro-inflammatory factors. Moreover, the presence of single nucleotide polymorphisms (SNPs) showed association with different production of immunoregulatory factors which may modulate the disease expression in AIHA. OBJECTIVES: To determine the importance of SNPs of pro- and anti-inflammatory factors in the development of AIHA. PATIENTS: We studied 17 patients with AIHA who has been followed in the Hematology and Blood Transfusion Unit at the Federal University of Sao Paulo (UNIFESP/EPM), Brazil. The control group was composed by 40 healthy volunteer blood donors. METHODS: After DNA extraction from peripheral blood samples, the frequency of the SNPs was determinate by PCR-RFLP in patients and healthy individuals. The following SNPs were analyzed: Interleukin 12: IL-12 1188 (A/C), Tumor Necrosis Factor alpha: TNFa-308 (G/A), and Lymphotoxin alpha: Lta +252 (A/G); Interleukin 10: IL-10-592 (C/A), and Cytotoxic T-lymphocyte associated protein 4: CTLA4 exon 1 49 (A/G). RESULTS: The patient group was composed predominantly by female individuals (14 or 82%) and the median of age was 56 years old (18 to 76 years). The frequency observed for each allele studied in the patient group was: allele A of IL-12 = 0.82; allele G of FNTa = 0.85; allele A of Lta = 0.68; allele C of IL-10 = 0.82; allele G of CTLA4 = 0.59. No differences in allele frequency were found between patient and control groups. CONCLUSIONS: Our results suggest that these polymorphisms appear not to contribute for the development of the AIHA.

scholarly journals Antitumour and Anti-Inflammatory Effects of Palladium(II) Complexes on Ehrlich Tumour

2013 ◽  
Vol 68 (7-8) ◽  
pp. 293-301
Author(s):  
Marcela B. Quilles ◽  
Camila B. A. Carli ◽  
Sandra R. Ananias ◽  
Lucas S. Ferreira ◽  
Livia C. A. Ribeiro ◽  
...  
Keyword(s):  
Mutagenic Activity ◽  
Interleukin 10 ◽  
Interleukin 12 ◽  
Tnf Α ◽  
Ehrlich Ascites Tumour ◽  
Eat Cells ◽  
Anti Inflammatory ◽  
Diphenyl Tetrazolium Bromide ◽  
Factor Α

Palladium(II) complexes are an important class of cyclopalladated compounds that play a pivotal role in various pharmaceutical applications. Here, we investigated the antitumour, anti-inflammatory, and mutagenic effects of two complexes: [Pd(dmba)(Cl)tu] (1) and [Pd(dmba)(N3)tu] (2) (dmba = N,N-dimethylbenzylamine and tu = thiourea), on Ehrlich ascites tumour (EAT) cells and peritoneal exudate cells (PECs) from mice bearing solid Ehrlich tumour. The cytotoxic effects of the complexes on EAT cells and PECs were assessed using the 3-(4,5-dimethylthiazol-3-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay. The effects of the complexes on the immune system were assessed based on the production of nitric oxide (NO) (Griess assay) and tumour necrosis factor-α (TNF-α), interleukin-12 (IL-12), and interleukin-10 (IL-10) (ELISA). Finally the mutagenic activity was assessed by the Ames test using the Salmonella typhimurium strain TA 98. Cisplatin was used as a standard. The IC50 ranges for the growth inhibition of EAT cells and PECs were found to be (72.8 ± 3.23) μM and (137.65 ± 0.22) μM for 1 and (39.7 ± 0.30) μM and (146.51 ± 2.67) μM for 2, respectively. The production of NO, IL-12, and TNF-α, but not IL-10, was induced by both complexes and cisplatin. The complexes showed no mutagenicity in vitro, unlike cisplatin, which was mutagenic in the strain. These results indicate that the complexes are not mutagenic and have potential immunological and antitumour activities. These properties make them promising alternatives to cisplatin

Interleukin-12 and interleukin-10 production by mononuclear phagocytic cells from breast cancer patients

1999 ◽  
Vol 68 (2-3) ◽  
pp. 355-358 ◽  
Author(s):  
R.A. Merendino ◽  
S. Gangemi ◽  
A. Misefari ◽  
A. Arena ◽  
A.B. Capozza ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Patients ◽  
Interleukin 10 ◽  
Interleukin 12 ◽  
Breast Cancer Patients ◽  
Phagocytic Cells

scholarly journals Cytokine control in human endometrium

Reproduction ◽  
2001 ◽  
pp. 3-19 ◽  
Author(s):  
RW Kelly ◽  
AE King ◽  
HO Critchley
Keyword(s):  
Direct Action ◽  
Progesterone Receptors ◽  
Interleukin 10 ◽  
Prostaglandin Synthesis ◽  
Leukaemia Inhibitory Factor ◽  
Interleukin 12 ◽  
Prostaglandin E ◽  
Inflammatory Drugs ◽  
Cytokine Synthesis ◽  
Anti Inflammatory

Cytokines within endometrium participate in both menstruation and implantation but also contribute to the defence mechanisms of the mucosal epithelium. Endometrium is under the control of steroid hormones, particularly progesterone and, thus, control of cytokines by this steroid is important. Although appreciable numbers of progesterone receptors are not found in endometrial leucocytes, progesterone can modulate cytokines by acting on uterine cells expressing the receptor. The NFkappaB pathway is important in the control of cytokine synthesis and can modulate production of chemokines, matrix metalloproteinases and the inducible prostaglandin synthesis enzyme COX-2. NFkappaB activity can be inhibited by progesterone by either stimulating synthesis of IkappaB, the molecule that restrains NFkappaB in the cytosol, or after binding to the nuclear receptor, competing with NFkappaB for recognition sites on the relevant gene. In this way, progesterone can limit pro-inflammatory pathways. The major palliatives for endometrial dysfunctions such as menorrhagia and dysmenorrhoea have been the non-steroidal anti-inflammatory drugs that inhibit prostaglandin synthesis. Prostaglandins have major effects on cytokine production but the direct action of prostaglandin E on leucocytes is not a pro-inflammatory response but is to stimulate interleukin 10 and inhibit interleukin 12 synthesis. The likely effect of the non-steroidal anti-inflammatory drugs is on the cells surrounding the small blood vessels, where a synergistic action between prostaglandin and chemokine will induce leucocyte entry and activation leading to lysis of connective tissue and menstruation. At the time of implantation, tight control of cytokine synthesis is required. Although leukaemia inhibitory factor is essential to implantation, the mouse knockout models show that the prostaglandin system is also essential but that there are mutually supportive pathways that compensate for the knockout of many cytokines.

scholarly journals Effect of corvitin on changes in cytokin levels in the development of experimental bronchial asthma in combination with adrenaline miocardial damage

2021 ◽  
Vol 11 (2) ◽  
pp. 308-316
Author(s):  
Nazariy Nebelyuk
Keyword(s):  
Bronchial Asthma ◽  
Structural Changes ◽  
Solid Phase ◽  
Myocardial Damage ◽  
Interleukin 10 ◽  
Common Disease ◽  
Necrosis Factor Alpha ◽  
Tnf Α ◽  
Cytokine Levels ◽  
Anti Inflammatory

Bronchial asthma (BA) is a fairly common disease of the bronchopulmonary system. Its share is from 0.6 to 2% of all respiratory pathology, in different countries this pathology affects from 1 to 10% of the population, in general, the world has at least 2% of the total population.Cardiovascular diseases continue to be a serious problem in medicine. The reason for this is a large number of stresses of various origins, which cause disruption of the body's adaptive capacity, the development of functional and structural changes in it, which ultimately lead to necrotic myocardial damage.The problem of pathogenesis, early diagnosis and treatment of BA today is not fully understood, it is still one of the serious issues of modern medicine. Despite lengthy research, the serious costs of research, the participation of the world's leading experts, methods of prevention and treatment of cardiovascular diseases, and rehabilitation of patients remain imperfect, although some progress and serious results have already been achieved.The aim of our study was to find out how cytokine levels will change at different stages of the formation of experimental asthma (EA) and adrenaline myocardial damage (AMD), what will be the indicators in the combination of these pathologies and what will be the effect of Corvitin on disorders combination of EA and AMD.The study was conducted on 127 guinea pigs. Blood sampling was performed on the 1st, 4th, 18th and 25th days of EA development, on the same days in AMD, as well as in combination with EA with AMD, and on the 25th day of development of these diseases with treatment. Corvitin, the level of pro-inflammatory cytokines - tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) and anti-inflammatory - interleukin-10 (IL-10) was determined by solid-phase enzyme-linked immunosorbent assay.Studies of cytokine levels in different groups of animals (intact, with EA, with AMD, with EA and AMD and in animals after treatment) showed that in animals with diseases, the rate of pro-inflammatory IL-6 and TNF-α increased with decreasing IL-10. At a combination of pathologies changes were the greatest that testifies to a course of diseases more difficult for an organism.The use of Corvitin showed a positive effect on the violation of cytokine levels and their imbalance, which was due to its antioxidant, anti-inflammatory and immunomodulatory effects. Therefore, quercetin may be effective in the treatment of asthma or a combination of pathologies.

Balance between interleukin-10 and interleukin-12 in adult cancer patients with or without infections

2001 ◽  
Vol 37 (7) ◽  
pp. 857-861 ◽  
Author(s):  
R Kallio ◽  
H.-M Surcel ◽  
A Bloigu ◽  
H Syrjälä
Keyword(s):  
Cancer Patients ◽  
Interleukin 10 ◽  
Interleukin 12
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