scholarly journals In VitroBiocompatibility of Electrospun Chitosan/Collagen Scaffold

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Peiwei Wang ◽  
Junli Liu ◽  
Teng Zhang
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Endothelial Cells ◽  
Cell Adhesion Molecules ◽  
Vascular Tissue ◽  
Collagen Scaffold ◽  
Nanofibrous Scaffold ◽  
Vascular Tissue Engineering ◽  
Rt Pcr ◽  
Composite Nanofiber

Chitosan/collagen composite nanofibrous scaffold has been greatly concerned in recent years for its favorable physicochemical properties which mimic the native extracellular matrix (ECM) both morphologically and chemically. In a previous study, we had successfully fabricated nanofibrous chitosan/collagen composite by electrospinning. In the present study, we further investigate the biocompatibility of such chitosan/collagen composite nanofiber to be used as scaffolds in vascular tissue engineering. The porcine iliac artery endothelial cells (PIECs) were employed for morphogenesis, attachment, proliferation, and phenotypic studies. Four characteristic EC markers, including two types of cell adhesion molecules, one proliferation molecule (PCNA), and one function molecule (p53), were studied by semiquantitative RT-PCR. Results showed that the chitosan/collagen composite nanofibrous scaffold could enhance the attachment, spreading, and proliferation of PIECs and preserve the EC phenotype. Our work provides profound proofs for the applicable potency of scaffolds made from chitosan/collagen composite nanofiber to be used in vascular tissue engineering.

Vessel graft fabricated by the on-site differentiation of human mesenchymal stem cells towards vascular cells on vascular extracellular matrix scaffold under mechanical stimulation in a rotary bioreactor

2019 ◽  
Vol 7 (16) ◽  
pp. 2703-2713 ◽  
Author(s):  
Na Li ◽  
Alex P. Rickel ◽  
Hanna J. Sanyour ◽  
Zhongkui Hong
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Extracellular Matrix ◽  
Mesenchymal Stem Cells ◽  
Mechanical Stimulation ◽  
Vascular Tissue ◽  
Human Mesenchymal Stem Cells ◽  
Stem Cell Differentiation ◽  
Vascular Tissue Engineering ◽  
Extracellular Matrix Scaffold

Stem cell differentiation on a decellularized native blood vessel scaffold under mechanical stimulation for vascular tissue engineering.

Endothelial cells (ECs) for vascular tissue engineering: venous ECs are less thrombogenic than arterial ECs

2012 ◽  
Vol 9 (5) ◽  
pp. 564-576 ◽  
Author(s):  
I. L. A. Geenen ◽  
D. G. M. Molin ◽  
N. M. S. van den Akker ◽  
F. Jeukens ◽  
H. M. Spronk ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Endothelial Cells ◽  
Vascular Tissue ◽  
Vascular Tissue Engineering

Tubular Poly(ε-caprolactone)/Chitosan Nanofibrous Scaffold Prepared by Electrospinning for Vascular Tissue Engineering Applications

2017 ◽  
Vol 7 (6) ◽  
pp. 427-436 ◽  
Author(s):  
Mohammed FayezAL Rez ◽  
Abdullah Binobaid ◽  
Abdulmajeed Alghosen ◽  
Eraj Humayun Mirza ◽  
Javed Alam ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Vascular Tissue ◽  
Nanofibrous Scaffold ◽  
Vascular Tissue Engineering ◽  
Engineering Applications

scholarly journals Mechanocompatible Polymer-Extracellular-Matrix Composites for Vascular Tissue Engineering

2016 ◽  
Vol 5 (13) ◽  
pp. 1594-1605 ◽  
Author(s):  
Bin Jiang ◽  
Rachel Suen ◽  
Jiao-Jing Wang ◽  
Zheng J. Zhang ◽  
Jason A. Wertheim ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Vascular Tissue ◽  
Vascular Tissue Engineering ◽  
Matrix Composites

scholarly journals Mitigating the non-specific uptake of immunomagnetic microparticles enables the extraction of endothelium from human fat

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Jeremy A. Antonyshyn ◽  
Vienna Mazzoli ◽  
Meghan J. McFadden ◽  
Anthony O. Gramolini ◽  
Stefan O. P. Hofer ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Endothelial Cells ◽  
Adipose Tissue ◽  
Stromal Cells ◽  
Reliable Method ◽  
Vascular Tissue ◽  
Human Adipose Tissue ◽  
Vascular Tissue Engineering ◽  
Microvascular Endothelial Cells ◽  
Specific Uptake

AbstractEndothelial cells are among the fundamental building blocks for vascular tissue engineering. However, a clinically viable source of endothelium has continued to elude the field. Here, we demonstrate the feasibility of sourcing autologous endothelium from human fat – an abundant and uniquely dispensable tissue that can be readily harvested with minimally invasive procedures. We investigate the challenges underlying the overgrowth of human adipose tissue-derived microvascular endothelial cells by stromal cells to facilitate the development of a reliable method for their acquisition. Magnet-assisted cell sorting strategies are established to mitigate the non-specific uptake of immunomagnetic microparticles, enabling the enrichment of endothelial cells to purities that prevent their overgrowth by stromal cells. This work delineates a reliable method for acquiring human adipose tissue-derived microvascular endothelial cells in large quantities with high purities that can be readily applied in future vascular tissue engineering applications.

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