scholarly journals PCR-Based Detection and Genotyping ofHelicobacter pyloriin Endoscopic Biopsy Samples from Brazilian Patients

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Silvia M. Ferreira Menoni ◽  
Sandra Helena Alves Bonon ◽  
José Murilo Robilota Zeitune ◽  
Sandra Cecília Botelho Costa
Keyword(s):  
Gastrointestinal Symptoms ◽  
Restriction Enzymes ◽  
Endoscopic Biopsy ◽  
Future Research ◽  
Rapid Urease Test ◽  
Computational Program ◽  
Urease Test ◽  
Precise Diagnosis ◽  
H Pylori ◽  
Molecular Biology Method

Helicobacter pylori(H. pylori) is considered the second most prevalent infection in man. A precise diagnosis is important for treating patients with the indicative gastrointestinal symptoms. The present study analyzes the effectiveness of a molecular biology method (PCR) comparing the results obtained with the histology and with the rapid urease tests. PCR was used in the detection and genotyping of theH. pyloriurease-C gene and the patterns which were obtained from the patients studied. 141 biopsy samples from 131 patients were evaluated. 59 paraffin biopsies samples were positive forH. pyloriaccording to the histological examination. Of those, 59/12 (20.3%) were amplified using PCR. Of the 82 samples from the fresh biopsies, 64 were positive forH. pyloriaccording to the rapid urease test (78%); there was an agreement of 100% with PCR. Sixty positiveH. pylorisamples were genotyped (58 samples of fresh biopsies and 2 samples of paraffin biopsies) using two restriction enzymes. The patterns observed were analyzed with the computational program BIO 1D; 11 patterns with the enzymeHhaIand 12 patterns with the enzymeMboIwere found. However, it was not possible to find a statistically significant correlation between the specific genotypes and digestive pathologies. Accordingly, future research should be performed to confirm a statistically significant relationship between genotyping and gastrointestinal symptoms.

scholarly journals Urine-Based ELISA for the Detection of Helicobacter pylori IgG Antibody and Comparison with Other Invasive Methods.

1970 ◽  
Vol 4 (1) ◽  
pp. 14-17 ◽  
Author(s):  
Md Din-ul Islam ◽  
Sufi HZ Rahman ◽  
SM Shamsuzzaman ◽  
Naima Muazzaman ◽  
Nasim Ahmed ◽  
...  
Keyword(s):  
Endoscopic Biopsy ◽  
Rapid Urease Test ◽  
Igg Antibody ◽  
Medical College ◽  
Urease Test ◽  
Study Population ◽  
H Pylori ◽  
Sensitivity Specificity ◽  
Culture Positive ◽  
Upper Gastrointestinal

The present study was conducted in the department of Microbiology, Dhaka Medical College, Dhaka during the period of January, 2007 to December, 2007. Urine samples were collected from 86 dyspeptic patients undergoing upper Gastrointestinal Tract (GIT) endoscopy to determine anti-H. pylori IgG antibody by an ELISA method. Gastric biopsy tissues were tested for culture, rapid urease test and H&E/Giemsa stain. Out of 86 endoscopic biopsy specimens, 45 (52.33%) were culture positive, 63 (73.26%) were rapid urease test positive and 64 (74.42%) were H&E/Giemsa stained positive for H. pylori. According to operational standard definition, among the 86 study population, 66 (76.74%) were H. pylori infected, 16 (18.60%) were uninfected and 4 (4.65%) were indeterminate. Among 66 H. pylori infected cases, 63 (95.45%) were urine ELISA positive and among 16 uninfected cases 3 (18.75%) were urine ELISA positive. Out of 86 study population, 66 (76.74%) were urine ELISA positive. The sensitivity, specificity, PPV, NPP and accuracy of urine ELISA were 95.45%, 81.25%, 95.45%, 81.25% and 92.68% respectively. The result of the study shows that H. pylori infection can be rapidly and reliably diagnosed by detecting anti-H. pylori IgG from urine. Key words: H. pylori; ELISA; GIT; IgG. DOI: http://dx.doi.org/10.3329/bjmm.v4i1.8463 BJMM 2011; 4(1): 14-17

scholarly journals Prevalence and genotyping of Helicobacter pylori in endoscopic biopsy samples from a Chinese population

2019 ◽  
Vol 43 (1) ◽  
pp. 21-28
Author(s):  
Hao Yu ◽  
Yingjia Mao ◽  
Lijie Cong ◽  
Zhiyong Wang ◽  
Hua Zhang ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Gastric Mucosa ◽  
Real Time ◽  
Chinese Population ◽  
Rflp Analysis ◽  
Endoscopic Biopsy ◽  
Gastric Biopsy ◽  
Rapid Urease Test ◽  
Urease Test ◽  
H Pylori

Abstract Background: Helicobacter pylori inhabit the gastric mucosa of humans and are associated with several gastrointestinal diseases which include gastric cancer, peptic ulcer, chronic gastritis and gastric mucosa-associated lymphoid tissue lymphoma. Helicobacter pylori exhibit a high degree of genetic variability and are associated with its epidemiological, pathological characteristics and dynamics of transmission. The objective of the study was to determine the prevalence and genetic heterogeneity of H. pylori isolated from endoscopic biopsy samples from a Chinese population. Methods: Gastric biopsy samples from 86 patients (males, 55; females, 35) who presented to the endoscopic section for various gastrointestinal abnormalities were collected. The samples were subjected to a real-time polymerase chain reaction (PCR) and microbial culture for the isolation of H. pylori. Further, the isolates were subjected to randomly amplified polymorphic DNA (RAPD) and restriction fragment length polymorphism (RFLP) analysis. Results: Of the 86 gastric biopsy samples, 61 (70.9%) samples were positive for rapid urease test and 37 (43%) samples (28 from male and nine from female) grew H. pylori. Among the biopsy samples subjected to real-time PCR, 39 (45.3%) samples were found to be positive for H. pylori. The RAPD analysis yielded 15 different patterns (four to 17 different sized fragments per strain). The phylogenetic analysis of RAPD yielded 22 clusters at a similarity level ranging from 63% to 100%. RFLP analysis yielded nine different patterns (two to six different sized fragments per strain). Two major restriction patterns were identified, of which 14 (37.8%) strains forms the most common pattern (genotype I) followed by five (13.5%, genotype II) strains with an intra-strain similarity of 100%. Conclusions: The overall prevalence of H. pylori was 45.3%. Despite reports on the declining trend in the prevalence of H. pylori infections, our prevalence rate was still higher than those reported from other developed countries. However, further studies involving a large sample size and covering more regions of China is highly warranted.

scholarly journals A Comparative Study between Helicobacter Pylori Antigen in Stool with Histopathology and Rapid Urease Test of Endoscopic Biopsy in PUD Patient

2021 ◽  
Vol 9 (2) ◽  
pp. 19-25
Author(s):  
Md Nazrul Islam Chowdhury ◽  
Syeed Mehbub Ul Kadir ◽  
Qamrun Naher ◽  
Lokesh Chakraborty ◽  
Mohammad Shahadat Hossain ◽  
...  
Keyword(s):  
Diagnostic Tool ◽  
Endoscopic Biopsy ◽  
Rapid Urease Test ◽  
Antigen Test ◽  
Cross Sectional ◽  
Stool Antigen Test ◽  
Ulcer Disease ◽  
Urease Test ◽  
Stool Antigen ◽  
H Pylori

Purpose: To assess stool antigen test as an early effective diagnostic tool like other methods for the diagnosis of H. pylori infection in PUD patient. Patients and Methods: This cross-sectional study was carried in a clinical pathology department of a tertiary level hospital. We included all patients with peptic ulcer disease those who were diagnosed by upper GIT endoscopy. Demographic variables and value of laboratory test including Stool antigen test, rapid urease test was studied in this study. Result: A total of eighty-six patients were undergone for endoscopy after tested for Helicobactor pylori antigen in stool. Among 86 study subjects, Male was 56 (65%) and female was 30 (35%). The Mean (±SD) age was found 38.53(±10.40) years with range from 21 to above 60 years. Stool antigen test positive in 66 (66/86, 76.74%), negative in 20 (20/86, 23.3%). Rapid Urease Test positive in 76 (76/86, 88.4%), negative in 20 (20/86, 11.6%). Histopathology positive in 65 (65/86, 75.58%), negative in 21 (21/86, 24.42%). The result was statistically highly significant (P<0.001). Conclusion: Stool antigen test (SAT) is an early effective diagnostic tool like other methods for the diagnosis of H. pylori infection in our country. CBMJ 2020 July: Vol. 09 No. 02 P: 19-25

Prevalence and genotyping of Helicobacter pylori in endoscopic biopsy samples from a Chinese population

2018 ◽  
Vol 0 (0) ◽  
Author(s):  
Hao Yu ◽  
Yingjia Mao ◽  
Lijie Cong ◽  
Zhiyong Wang ◽  
Hua Zhang ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Gastric Mucosa ◽  
Real Time ◽  
Chinese Population ◽  
Rflp Analysis ◽  
Endoscopic Biopsy ◽  
Gastric Biopsy ◽  
Rapid Urease Test ◽  
Urease Test ◽  
H Pylori

AbstractBackground:Helicobacter pylori inhabit the gastric mucosa of humans and are associated with several gastrointestinal diseases which include gastric cancer, peptic ulcer, chronic gastritis and gastric mucosa-associated lymphoid tissue lymphoma. Helicobacter pylori exhibit a high degree of genetic variability and are associated with its epidemiological, pathological characteristics and dynamics of transmission. The objective of the study was to determine the prevalence and genetic heterogeneity of H. pylori isolated from endoscopic biopsy samples from a Chinese population.Methods:Gastric biopsy samples from 86 patients (males, 55; females, 35) who presented to the endoscopic section for various gastrointestinal abnormalities were collected. The samples were subjected to a real-time polymerase chain reaction (PCR) and microbial culture for the isolation of H. pylori. Further, the isolates were subjected to randomly amplified polymorphic DNA (RAPD) and restriction fragment length polymorphism (RFLP) analysis.Results:Of the 86 gastric biopsy samples, 61 (70.9%) samples were positive for rapid urease test and 37 (43%) samples (28 from male and nine from female) grew H. pylori. Among the biopsy samples subjected to real-time PCR, 39 (45.3%) samples were found to be positive for H. pylori. The RAPD analysis yielded 15 different patterns (four to 17 different sized fragments per strain). The phylogenetic analysis of RAPD yielded 22 clusters at a similarity level ranging from 63% to 100%. RFLP analysis yielded nine different patterns (two to six different sized fragments per strain). Two major restriction patterns were identified, of which 14 (37.8%) strains forms the most common pattern (genotype I) followed by five (13.5%, genotype II) strains with an intra-strain similarity of 100%.Conclusions:The overall prevalence of H. pylori was 45.3%. Despite reports on the declining trend in the prevalence of H. pylori infections, our prevalence rate was still higher than those reported from other developed countries. However, further studies involving a large sample size and covering more regions of China is highly warranted.

Rapid urease test for H. pylori diagnosis: pros and cons

2010 ◽  
Vol 5 (3) ◽  
pp. 257-258 ◽  
Author(s):  
Angelo Zullo ◽  
Cesare Hassan ◽  
Silvia Trapani ◽  
Gianfranco Tammaro
Keyword(s):  
Rapid Urease Test ◽  
Urease Test ◽  
Pros And Cons ◽  
H Pylori

Frequency of rdx A Deletion Mutation Conferring Metronidazole Resistance in Helicobacter pylori

2020 ◽  
Vol 29 (3) ◽  
pp. 59-64
Author(s):  
Hanaa M. El Maghraby ◽  
Samar Mohaseb
Keyword(s):  
Helicobacter Pylori ◽  
Epigastric Pain ◽  
Deletion Mutation ◽  
Rapid Urease Test ◽  
Rrna Gene ◽  
University Hospitals ◽  
Metronidazole Resistance ◽  
Urease Test ◽  
Gastric Biopsies ◽  
H Pylori

Background: Metronidazole is one of the antimicrobial drugs that can be used in combination with other drugs for eradication of Helicobacter pylori (H. pylori).Unfortunately, metronidazole resistance in H. plori is an increasing health problem which may be attributed to inactivation of many genes as rdx A gene. Objective: To determine the frequency of rdx A deletion mutation in H. pylori detected in infected patients attending at the Gastroenterology Unit, Zagazig University Hospitals. Methodology: Two gastric biopsies were taken from each enrolled patient by endoscopy. H.pylori detection was done by rapid urease test and polymerase chain reaction (PCR) amplification of 16S rRNA gene. Deletion mutation in rdx A gene was detected by conventional PCR. Results: Out of 134 doubled gastric biopsies obtained from 134 patients, 52.2% were positive for H. pylori. Epigastric pain, vomiting and gastritis were significantly associated with detection of H. pylori infection (p˂ 0.05). Deletion mutation of rdx A gene was detected in 28.6% of H. pylori positive specimens obtained from infected patients. Conclusion: Deletion mutation of rdx A gene is a frequent determinant of rdx A inactivation conferring metronidazole resistance among H. pylori.

scholarly journals Incidence of Gastric Metaplasia and Helicobacter pylori Infection in Duodenal Bulb – With Specific Reference to Patients With Duodenal Ulcers

1999 ◽  
Vol 6 (1) ◽  
pp. 17-23 ◽  
Author(s):  
Minoru Kawaguchi ◽  
Toshihiko Saito
Keyword(s):  
Helicobacter Pylori ◽  
Duodenal Bulb ◽  
Mucosal Injury ◽  
Transitional Zone ◽  
Specific Reference ◽  
Rapid Urease Test ◽  
Duodenal Ulcers ◽  
Gastric Metaplasia ◽  
Urease Test ◽  
H Pylori

We determined the incidence of gastric metaplasia in the duodenal bulb of duodenal ulcer patients and the Helicobacter pylori (H. pylori) infection rate at sites with gastric metaplasia. Biopsy of the duodenal bulb showed the presence of gastric metaplasia in 61 of 86 patients (71%) overall and in 18 of 47 patients (38.3%) who had gastrectomy at an early gastric cancer. The histological diagnosis of H. pylori infection showed good agreement (83.3%) with the result of the rapid urease test, indicating that H. pylori occurs in regions with gastric metaplasia. This finding suggests that H. pylori infects gastric metaplasia in the duodenal bulb, causing mucosal injury, which is then transformed into duodenal ulcers. The exact mechanism by which gastric metaplasia is caused is unknown, but it is believed to occur in the transitional zone in the duodenal mucosa.

scholarly journals Standard Triple Therapy versus Sequential Therapy inHelicobacter pyloriEradication: A Double-Blind, Randomized, and Controlled Trial

2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Jaime Natan Eisig ◽  
Tomás Navarro-Rodriguez ◽  
Ana Cristina Sá Teixeira ◽  
Fernando Marcuz Silva ◽  
Rejane Mattar ◽  
...  
Keyword(s):  
Triple Therapy ◽  
Controlled Trial ◽  
Sequential Therapy ◽  
Rapid Urease Test ◽  
Double Blind ◽  
Intention To Treat ◽  
Standard Triple Therapy ◽  
Urease Test ◽  
H Pylori ◽  
Triple Treatment

Aim. To compare 10-day standard triple therapy versus sequential therapy as first-line treatment in patients infected withH. pylori.Methods. One hundredH. pyloripositive patients (diagnosed by rapid urease test and histology), with average age of 47.2, M/F = 28/72, were randomized to receive either standard triple treatment (TT) as follows: lansoprazole 30 mg, clarithromycin 500 mg, and amoxicillin 1 g, b.i.d. for ten days, or sequential treatment (ST) as follows: lansoprazole 30 mg, amoxicillin and placebo 1.0 g b.i.d for the first five days, followed by lansoprazole 30 mg, clarithromycin 500 mg, and tinidazole 500 mg b.i.d, for the remaining five days. Eradication rates were determined 60 days after treatment by urease, histology, or13C-urea breath test.Results. In intention to treat (ITT) analysis, the rate ofH. pylorieradication in the TT and ST groups was the same for both regimens as follows: 86% (43/50), 95% CI 93,3 to 73.4%. In Per protocol (PP) analysis, the rate ofH. pylorieradication in the TT and ST groups was 87.8% (43/49), 95% CI 94,5 to 75.3% and 89.6% (43/48), 95% CI 95,8 to 77.3%, respectively.Conclusions. In Brazil, standard triple therapy is as equally effective as sequential therapy in eradicatingHelicobacter pyloripatients. This study was registered under Clinical Trials with numberISRCTN62400496.

scholarly journals GASTROESOPHAGEAL REFLUX DISEASE

2011 ◽  
Vol 18 (04) ◽  
pp. 693-696
Author(s):  
ABDUL RAZZAQ WARRAICH ◽  
DR. RAFFAD ◽  
CHAUDHRY MUHAMMAD SIDDIQ ◽  
Zaheer Iqbal ◽  
Mohammad Saeed Khokhar
Keyword(s):  
Gastroesophageal Reflux Disease ◽  
Gastroesophageal Reflux ◽  
Reflux Disease ◽  
Rapid Urease Test ◽  
Common Symptom ◽  
Infection Frequency ◽  
Urease Test ◽  
H Pylori ◽  
Disease Study ◽  
Study Setting

Objective: To find the frequency of H pylori infection among the patients of gastroesophgeal reflex disease. Study design: Descriptive study. Setting: Department of Medicine, Unit-I, Services Hospital, Lahore. Subject & method: One hundred patients with gastroesophageal reflux disease (GERD) were included in this study. For detection and confirmation of helicobacter pylori, endoscopy with antral biopsy was done. Specimens were sent for histopathology, and rapid urease test was performed. Main outcome measures: Frequency of H pylori infection, frequency of regurgitation, dysphagia, nausea, vomiting and haemetemesis. Results: 100 patients who fulfilled the inclusion criteria were taken. There were 65 males and 35 females respectively. Their age ranged from 18 to 48 years. 39 (39%) patients were between 29-38 years while 23 (23%) were of 39-48 years. There were 38 (38%) patients between 18-28 years. The mean age of the patients was 31.79±7.56. 40 (40%) were found to be H. pylori positive on biopsy and rapid urease test, while 60 (60%) were H. pylori negative. The most common recorded symptom was chest pain/retrosternal burning (90%). Regurgitation (65%) and nausea (55%) were other common symptoms. Dysphagia and water-brash were present in 40% each. Vomiting (30%), abdominal pain (25%) and cough (20%) were the other symptoms. The least common symptom was haemetemesis (5%). Conclusions: A causal relationship between GERD and H. pylori infection could not be established. Therefore, routine H. pylori eradication in the treatment of patients with GERD is not recommended. 

scholarly journals Eradication of Helicobacter pylori in patients without gastric symptoms suffering from recurrent aphthous stomatitis: A pilot study

2017 ◽  
Vol 74 (7) ◽  
pp. 672-675 ◽  
Author(s):  
Marina Latkovic ◽  
Lazar Ranin ◽  
Nevenka Teodorovic ◽  
Marko Andjelkovic
Keyword(s):  
Eradication Therapy ◽  
Recurrent Aphthous Stomatitis ◽  
Aphthous Stomatitis ◽  
Diffusion Method ◽  
Gastric Disease ◽  
Warning Sign ◽  
Rapid Urease Test ◽  
Sensitivity Testing ◽  
Urease Test ◽  
H Pylori

Background/Aim. Helicobacter (H.) pylori is a widespread bacterium and its involvement in pathogenesis of gastric diseases is well-known. However, H. pylori role in etiology of other histologically similar conditions, especially recurrent aphthous stomatitis (RAS) is still controversial. Research regarding H. pylori and its association with RAS, as well as the treatment options were always conducted on patients with diagnosed gastric problems. The aim of this study was to determine whether H. pylori is present in the oral cavity of patients suffering from RAS but without any symptoms or medical history of gastric disease. Methods. A total of 15 patients with RAS participated in the study. None of the participants suffered from any gastrointestinal disorders. Two dental plaque samples from each participant were collected. The first was analyzed using rapid urease test and the second one was put in transport medium and sent for cultivation. The sensitivity of H. pylori to antibiotics was established using disk diffusion method of sensitivity testing for every patient individually and adequate therapy was prescribed. Results. Before the treatment the mean annual recurrence rate of RAS was 8.1 ? 2.1, with the average number of lesions being 3.9 ? 1.9. During the 12-month observation period after the eradication therapy, none of the patients reported recurrence of aphthous lesions. The treatment was successful in all cases. Conclusion. This study shows that RAS can be effectively treated by successful eradication of oral H. pylori, and that RAS could be possibly considered as an early warning sign of potential gastric infection by H. pilory.

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