scholarly journals TheConyza trilobaExtracts with High Chlorophyll Content and Free Radical Scavenging Activity Had Anticancer Activity in Cell Lines

2013 ◽  
Vol 2013 ◽  
pp. 1-11 ◽  
Author(s):  
Wael M. El-Sayed ◽  
Warda A. Hussin ◽  
Ahmed A. Mahmoud ◽  
Mohamed A. AlFredan
Keyword(s):  
Free Radical ◽  
Chlorophyll Content ◽  
Cell Lines ◽  
Anticancer Agents ◽  
Methylene Chloride ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Antimutagenic Activity ◽  
Metal Chelating

The discovery of anticancer agents paradigm has been shifted to natural resources to overcome the toxicity of many synthetic agents at early clinical stages. In the present study, the antimutagenic, anticancer, phytochemistry, and free radical scavenging activities of five extracts ofConyza trilobawere investigated. Extracts II (water : methanol), III (methylene chloride), and IV (methylene chloride : methanol) had the highest chlorophyll content and the highest superoxide scavenging, and metal chelating activities comparable to that of trolox. They also showed DPPH•scavenging activities better than that ofα-tocopherol. Virtually all extracts exerted a strong (>40% reduction) antimutagenic activity against sodium azide and benzopyrene. Extracts II, III, and IV showed a remarkable growth inhibition profile with GI50of 0.07–0.87 μg for Hepa1c1c7 and H4IIE1, A549, HT29, and PC3 cell lines and totally abated the growth of all cell lines, except for the breast cells, at 0.3–7.0 μg. The present study found a strong correlation between the chlorophyll content ofConyzaextracts and their DDPH scavenging, metal chelating, and in vitro cytotoxic and cytostatic activities most probably through triggering apoptosis. This study could offer a platform for future studies and help selecting the vital features that identify the extract with potential anticancer activities.

scholarly journals PHYTOCHEMICAL ANALYSIS, ASSESSMENT OF ANTIPROLIFERATIVE AND FREE RADICAL SCAVENGING ACTIVITY OF MORUS ALBA AND MORUS RUBRA FRUITS

2017 ◽  
Vol 10 (6) ◽  
pp. 189
Author(s):  
Farouk K El-baz ◽  
Amal Z Hassan ◽  
Howaida I Abd-alla ◽  
Hanan F Aly ◽  
Khaled Mahmoud
Keyword(s):  
Fatty Acid ◽  
Free Radical ◽  
Chemical Composition ◽  
Cytotoxic Activity ◽  
Cell Lines ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Scavenging Activity ◽  
Acid Methyl

Objective: Mulberry is a nontoxic commonly eaten plant, belongs to the Morus and used in folk medicine in the remedy of dysentery, antiphlogistic, diuretic, expectorant, and antidiabetic. The purpose of this study is to evaluate the antiproliferative and radical scavenging activity of the total alcoholic and successive fractions thereof of Morus alba and Morus rubra fruits. In addition, the chemical composition of the bioactive fractions of each species was investigated.Methods: The antiproliferative potential of 8 extracts on 4 human cancer cell lines, hepatocellular carcinoma (HepG2), Caucasian breast adenocarcinoma (MCF7), prostate (PC3), and colon carcinoma (HCT116) in addition to one normal cell line namely human normal immortalized skin fibroblast cells (BJ1) were carried out. Cell viability was determined using MTT assay. The potency was compared with the reference drug doxorubicin. These extracts were also assayed for 1,1-diphenyl-2-hydrazyl free radical scavenging activities. After saponification of the n-hexane fraction, unsaponifiable matter and fatty acid methyl esters were analyzed by gas liquid chromatography (GLC). The chemical composition of the bioactive fractions was investigated using gas chromatography/mass spectrometry (GC/MS) analysis.Results: All the extracts showed significant free radical scavenging activity dose-dependently. The n-hexane and dichloromethane (DCM) fractions of M. rubra exhibited potent cytotoxic activity on almost cancer cell lines. In the same pattern, ethyl acetate (EtOAc) of M. rubra has moderate cytotoxic activity against all cell lines except HepG2. DCM fraction of M. alba possessed both radical scavenging and high potential antiproliferated activities against HCT116 and MCF7 with inhibitory concentration of 43.9 and 32.3 μg/ml, respectively, while it showed no cytotoxic effect on BJ1. GLC analysis showed the major hydrocarbons in M. alba and M. rubra were heptacosane and docosane, respectively. Sterols were similar in both species but with different ratios and cholesterol was the major one. Palmitic and margaric were the major saturated fatty acid while arachidonic was the major unsaturated fatty acid in both species. GC/MS analysis showed the main compound in DCM fraction of each Morus species was palmitic acid. Furthermore, 1,11-bis-(methoxycarbonyl-ethenyl)-10,2-dihydroxy-cycloeicosane and linolelaidic acid, methyl ester were the main compounds in the EtOAc fraction of each Morus species. Whereas, the main compounds in alcoholic extract of M. alba and M. rubra were methyl-14-methyl-pentadecanoate and 1,2-O-isopropylyidene-4-nonene-1,2,3-triol, respectively.Conclusions: The results observed remarkable biological activity of the successive fractions of M. rubra more than those of M. alba and confirmed its importance as a natural bioactive source. Morus species are good candidates to be promising as possible sources for future antitumor and antioxidants in food and pharmaceutical formulations. The strong activity partly explains the potential effects of Morus species for the treatment of cancer and degenerative diseases caused by free radicals.

scholarly journals Radical Scavenging Activity of Edible Fungal Sporocarp Extracts from the Niger Delta Region of Nigeria

2017 ◽  
Vol 9 (4) ◽  
Author(s):  
Ita B. N. ◽  
Eduok S. I.
Keyword(s):  
Free Radical ◽  
Niger Delta ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Scavenging Activity ◽  
Aqueous Extracts ◽  
Metal Chelating ◽  
Niger Delta Region

The radical scavenging activity of acetone, ethanol and aqueous extracts from Agaricus placomyces, Cantharellus cibarius and Pleurotus ostreatus from the Niger Delta region of Nigeria was determined with their metal chelating and reducing power. The extracts exhibited significant free radical scavenging activities in the DPPH assay (IC50 = 2.11 to 4.37 mg/mL) with the aqueous extract from P. ostreatus being the most potent. Also, the aqueous extracts exhibited better metal chelating abilities and reducing power than the ethanol and acetone extracts. In addition, higher content of total phenolics and flavonoids was found in the aqueous extract of A. placomyces (55.63mg GAE/g) and P. ostreatus (15.55 mgQE/g) respectively, whereas ascorbic acid ranged from 0.33 to 1.22 mgAAE/ in all the extracts. Overall, our results indicate that mushrooms particularly Agaricus placomyces, and Pleurotus ostreatus contain high amount of polyphenolic compounds with significant free radical scavenging abilities, notable metal chelating activities and promising reducing power. These properties indicate that the mushrooms are functional foods with the potential to attenuate oxidative stress associated with free radical damage in biological systems.

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