scholarly journals Effectiveness of Interstitial Laser Acupuncture Depends upon Dosage: Experimental Results from Electrocardiographic and Electrocorticographic Recordings

2013 ◽  
Vol 2013 ◽  
pp. 1-4 ◽  
Author(s):  
Wei He ◽  
Gerhard Litscher ◽  
Xiang-Hong Jing ◽  
Hong Shi ◽  
Xiao-Yu Wang ◽  
...  
Keyword(s):  
Heart Rate ◽  
Heart Rate Variability ◽  
Acupuncture Therapy ◽  
Experimental Results ◽  
Time Dependent ◽  
Laser Acupuncture ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Interstitial Laser ◽  
Electroencephalogram Eeg

The purpose of this study was to evaluate the influence of the duration of interstitial laser acupuncture therapy effects on neurovegetative and neurobioelectrical parameters like heart rate (HR), heart rate variability (HRV), and electroencephalogram (EEG). We investigated 6 male Sprague-Dawley rats. They underwent 10 min, 20 min, and 30 min interstitial laser acupuncture (in randomized order, with a break of at least 30 min between the different measurement conditions) at the acupoint Neiguan. HR changed significantly only during 20 min red laser stimulation, whereas 10 and 30 min stimulation did not induce significant changes. HRV did not change significantly during any of the different durations; however, an increase was found during 20 min irradiation. Neither the LF/HF ratio of HRV nor the integrated EEG showed significant changes. In this study, it could be experimentally proved that some effects of laser acupuncture are time dependent, and therefore the dosage, as well known from theory, also depends on the time factor. We could especially demonstrate that different treatment times lead to different effects on neurovegetative and neurobioelectrical parameters. Further studies are needed to verify or refute these results.

scholarly journals Intravenous Laser Blood Irradiation, Interstitial Laser Acupuncture, and Electroacupuncture in an Animal Experimental Setting: Preliminary Results from Heart Rate Variability and Electrocorticographic Recordings

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Wei He ◽  
Gerhard Litscher ◽  
Xiaoyu Wang ◽  
Xianghong Jing ◽  
Hong Shi ◽  
...  
Keyword(s):  
Heart Rate ◽  
Heart Rate Variability ◽  
Continuous Wave ◽  
Femoral Vein ◽  
Wave Mode ◽  
Laser Acupuncture ◽  
Sprague Dawley ◽  
Laser Stimulation ◽  
Blood Irradiation ◽  
Laser Blood Irradiation

This is the first study to investigate intravenous (i.v.) laser blood irradiation, interstitial (i.st.) laser acupuncture, and electroacupuncture (EA) in combination with heart rate variability (HRV) and electrocorticogram. We investigated 10 male anesthetized Sprague-Dawley rats under the three conditions mentioned previously in Beijing, China, and data analysis was performed in Graz, Europe. For i.v. laser stimulation in the femoral vein and i.st. laser acupuncture at Neiguan (PC6), we used a European system (Modulas needle, Schwa-Medico, Germany; 658 nm, 50 mW, continuous wave mode), and for EA at Neiguan, a Chinese system (Hanshi-100A; Nanjing Jisheng Medical Technology Company, China; 15 Hz, 1 mA). HR, HRV, and electrocorticogram were recorded using a biophysical amplifier AVB-10 (Nihon-Kohden, Japan). HR changed significantly during i.st. laser acupuncture stimulation of Neiguan in anesthetized rats. Total HRV increased insignificantly during i.v. and i.st. laser stimulation. The LF/HF ratio showed significant changes only during i.v. laser blood irradiation. Integrated cortical EEG (electrocorticogram) decreased insignificantly during EA and i.v. laser blood irradiation. Further studies concerning dosage-dependent alterations are in progress.

scholarly journals The Effect of Subdiaphragmatic Vagotomy on Heart Rate Variability and Lung Inflammation in Rats with Severe Hemorrhagic Shock

2021 ◽  
Author(s):  
Fateme Khodadadi ◽  
Farzaneh Ketabchi ◽  
Zahra Khodabandeh ◽  
Alireza Tavassoli ◽  
Gregory F. Lewis ◽  
...  
Keyword(s):  
Heart Rate ◽  
Heart Rate Variability ◽  
Hemorrhagic Shock ◽  
Hemodynamic Parameters ◽  
Sprague Dawley ◽  
Inflammatory Reactions ◽  
Sprague Dawley Rats ◽  
Tnf Α

Abstract Background The role of the sub-diaphragmatic branch of the vagus nerve in mediating heart rate variability (HRV) and inflammatory reaction to long term hemorrhagic shock has not been determined prior to this study. Methods Male Sprague-Dawley rats were divided into four groups of Sham, sub-diaphragmatic vagotomized (Vag), long term (130±2 minutes) hemorrhagic shock (LHS), and sub-diaphragmatic vagotomized with LHS (Vag+LHS). Hemodynamic parameters were recorded and HRV calculated during multiple phases of hemorrhagic shock. The expressions of TNF-α and iNOS were measured in the spleen and lung tissues at the conclusion of the protocol. Results Decreases in blood pressure during blood withdrawal were identical in the LHS and Vag+LHS groups. However, heart rate only decreased in the Nadir-1 phase of the LHS group. HRV indicated increased power in the very-low, low, and high (VLF, LF, and HF) frequency bands during the Nadir-1 phase of the LHS group and decreased power in the Vag+LHS group. There was metabolic acidosis partially compensated with respiratory system in the LHS and Vag+LHS groups. Increases of TNF-α and iNOS expression in the spleen and lung of the LHS group were reversed in the Vag+LHS group. Conclusion This study indicates that sub-diapragmatic vagotomy increases lung inflammatory reactions and blunts the cardiac vagal tone surge in response to severe hemorrhagic shock.

Heart rate variability and electrocardiogram waveform as predictors of morbidity during hypothermia and rewarming in rats

2002 ◽  
Vol 80 (9) ◽  
pp. 925-933 ◽  
Author(s):  
C B Matthew ◽  
A M Bastille ◽  
R R Gonzalez ◽  
I V Sils
Keyword(s):  
Blood Pressure ◽  
Heart Rate ◽  
Heart Rate Variability ◽  
Wave Amplitude ◽  
Sodium Pentobarbital ◽  
Sprague Dawley ◽  
T Wave ◽  
Sprague Dawley Rats ◽  
Copper Tubing ◽  
Electrocardiogram Ecg

This study examined electrocardiogram (ECG) waveform, heart rate (HR), mean blood pressure (BP), and HR variability as potential autonomic signatures of hypothermia and rewarming. Adult male Sprague–Dawley rats had telemetry transmitters surgically implanted, and 2 weeks were allowed for recovery prior to induction of hypothermia. Rats were lightly anesthetized (sodium pentobarbital, 35 mg/kg i.p.) and placed in a coil of copper tubing through which temperature-controlled water was circulated. Animals were cooled to a core temperature (Tc) of 20°C, maintained there for 30 min, and then rewarmed. Data (Tc, BP, HR from ECG, and 10-s strips of ECG waveforms) were collected every 5 min throughout hypothermia and rewarming. Both HR and BP declined after initial increases with the drop in HR starting at a higher Tc than the drop in BP (29.6 ± 2.4°C vs. 27.1 ± 3.3°C, p < 0.05). Animals that were not successfully rewarmed exhibited a significant (p < 0.05) increase in the normalized standard deviation of interbeat intervals (IBI) throughout cooling compared with animals that were successfully rewarmed. The T wave of the ECG increased in amplitude and area with decreasing Tc. T-wave amplitude and IBI variability show potential as predictors of survival in hypothermic victims.Key words: hypothermia, rewarming, heart rate variability, ECG, blood pressure.

scholarly journals Development of a Rat Model of Sick Sinus Syndrome Using Pinpoint Press Permeation

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Hong-bin Zhong ◽  
Ting-jun Wang ◽  
Gui-li Lian ◽  
Chang-sheng Xu ◽  
Hua-jun Wang ◽  
...  
Keyword(s):  
Heart Rate ◽  
Sinoatrial Node ◽  
Sick Sinus Syndrome ◽  
Pacemaker Implantation ◽  
Time Dependent ◽  
Severely Injured ◽  
Sprague Dawley ◽  
Limited Success ◽  
Sprague Dawley Rats ◽  
Common Causes

Objective. Sick sinus syndrome (SSS) is one of the most common causes of cardiac impairment necessitating pacemaker implantation. However, studies of SSS pathogenesis are neither comprehensive nor conclusive due to limited success in achieving a stable rat SSS model. Here, we modified pinpoint press permeation to establish a stable rat SSS model. Methods. We randomly assigned 138 male Sprague-Dawley rats into three groups: normal control (n = 8), sham (n = 10), and SSS (n = 120). Postoperatively, the SSS group was further divided into SSSA (n = 40), SSSB (n = 40), and SSSC (n = 40), based on reduction in heart rates by 20–30%, 31–40%, and 41–50%, respectively. We also assessed histomorphological characteristics and hyperpolarization-activated cyclic nucleotide-gated cation channel 4 (HCN4) expression in the sinoatrial node (SAN) at 1, 2, 3, and 4 weeks after surgery. Results. Mortality was statistically higher in SSSC compared to SSSA and SSSB (7.5% versus 90.0% and 87.5%; P < 0.05). Heart rate in SSSA was gradually restored to preoperative levels by week 4 after surgery. In contrast, heart rate in SSSB was stable at 2–3 weeks after surgery. However, we observed that the tissues and cells in SAN were severely injured and also found a time-dependent increase in collagen content and atrium myocardium in SSSB. HCN4 expression was significantly reduced at all 4 time points in SSSB, with statistically significant differences among the groups (P < 0.01). Conclusion. We successfully developed a rat SSS model that was sustainable for up to 4 weeks.

Estimation of Heart Rate Variability Fluctuations by Wavelet Transform

2011 ◽  
Vol 57 (3) ◽  
pp. 395-400 ◽  
Author(s):  
Anton Popov ◽  
Yevgeniy Karplyuk ◽  
Volodymyr Fesechko
Keyword(s):  
Heart Rate ◽  
Heart Rate Variability ◽  
Wavelet Transform ◽  
Experimental Results ◽  
Dyadic Wavelet ◽  
Heart Rate Fluctuations ◽  
Slow Heart Rate ◽  
Dyadic Wavelet Transform

Estimation of Heart Rate Variability Fluctuations by Wavelet TransformTechnique for separate estimation of fast and slow fluctuations in the heart rate signal is developed. The orthogonal dyadic wavelet transform is used to separate the slow heart rate changes in approximation part of decomposition and fast changes in detail parts. Experimental results using the recordings from persons practicing Chi meditation demonstrated the applicability of estimation heart rate fluctuations with the proposed approach.

scholarly journals Experimental Results on a New Method for Analysis of Heart Rate Variability

2014 ◽  
Vol 04 (08) ◽  
pp. 385-389 ◽  
Author(s):  
Elio Conte ◽  
William Giroldini ◽  
Vincenza Laterza ◽  
Sergio Conte ◽  
Maria Pieralice ◽  
...  
Keyword(s):  
Heart Rate ◽  
Heart Rate Variability ◽  
Experimental Results ◽  
New Method

A STANDARDIZED METHOD FOR THE PRODUCTION OF HEMORRHAGIC SHOCK IN THE RAT

1955 ◽  
Vol 33 (3) ◽  
pp. 436-447 ◽  
Author(s):  
H. G. Downie ◽  
J. A. F. Stevenson
Keyword(s):  
Blood Pressure ◽  
Heart Rate ◽  
Mortality Rate ◽  
Hemorrhagic Shock ◽  
Respiratory Rate ◽  
Rectal Temperature ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Standardized Method

Although the blood pressure is one of the important criteria in the standardization of hemorrhagic shock in the dog, it has rarely been used for this purpose in the rat. A method resembling the reservoir technique developed by Wiggers and Werle (1942) for the dog using blood pressure as the criterion has been modified for use with the rat. Male Sprague-Dawley rats weighing approximately 400 gm. were used. In the standardization of this technique the blood pressure was reduced to 30 mm. Hg in a 10-min. period of hemorrhage and then maintained at this level by subsequent small hemorrhages into the reservoir until reinfusion indicated the beginning of vascular collapse, at which time all the blood in the reservoir was returned. Considering that those animals which lived longer than 48 hr. were survivors, in a series of 27 animals, 21 died and 6 survived—a mortality rate of 78%.During the hypotensive period there was a consistent and steady drop in the respiratory rate and rectal temperature. The heart rate declined initially and tended to recover as the hypotensive period progressed. After reinfusion the blood pressure rose but. did not reach prehemorrhage levels. Hemorrhage into the bowel and convulsions were significant postreinfusion findings.

Dietary obesity and weight cycling in rats: a model of stress-induced hypertension?

1991 ◽  
Vol 261 (4) ◽  
pp. R848-R857 ◽  
Author(s):  
R. J. Contreras ◽  
S. King ◽  
L. Rives ◽  
A. Williams ◽  
T. Wattleton
Keyword(s):  
Heart Rate ◽  
Sodium Excretion ◽  
Mild Hypertension ◽  
Weight Cycling ◽  
Ang Ii ◽  
Sprague Dawley ◽  
Sweetened Condensed Milk ◽  
Condensed Milk ◽  
Sprague Dawley Rats ◽  
Dietary Obesity

The present study was designed to reproduce the mild hypertension seen in dietary obese weight-cycled rats [P. Ernsberger and D. O. Nelson. Am. J. Physiol. 254 (Regulatory Integrative Comp. Physiol. 23): R47-R55, 1988] and determine whether this mild hypertension was associated with changes in sodium excretion and pressor responsiveness to angiotensin II (ANG II). Male Sprague-Dawley rats were fed pelleted chow (Pellet group) or chow plus sweetened condensed milk (Milk group) or were exposed to four cycles of a 4-day fast alternated with 2 wk of refeeding of pelleted chow and sweetened condensed milk (Cycled group). Blood pressure and heart rate were measured by tail cuff at the onset and last day of each fast and after 3 days of refeeding. During fasting, urine sodium excretion was measured. Mean arterial pressure and heart rate responses to intravenous administration of ANG II (40, 80, and 120 ng/kg), metoprolol (1 mg/kg), and methyl scopolamine (2 mg/kg) were obtained from the femoral artery in awake unrestrained rats. Weight cycling did not lead to mild hypertension or increased bradycardic response to sympathetic blockade with metoprolol. ANG II-elicited pressor responses were similar for Pellet, Milk, and Cycled groups. Sodium excretion did not change with fasting. Mild hypertension developed when obese weight-cycled rats were housed together in groups and not when housed individually. Our preliminary data are consistent with the notion that stress associated with group housing may be a factor in the mild hypertension of obese weight-cycled rats.

Identification of spontaneous cardiac baroreflex episodes at different timescales in rats

1998 ◽  
Vol 274 (2) ◽  
pp. H488-H493 ◽  
Author(s):  
Marie-Paule Gustin ◽  
Catherine Cerutti ◽  
Robert Unterreiner ◽  
Christian Paultre
Keyword(s):  
Heart Rate ◽  
Sampling Interval ◽  
Statistical Relationship ◽  
Sprague Dawley ◽  
Time Intervals ◽  
Sprague Dawley Rats ◽  
Low Pass ◽  
Freely Moving ◽  
Spontaneous Baroreflex ◽  
Long Timescales

To study spontaneous cardiac baroreflex at different timescales, a new method has been developed that identifies such episodes. Mean arterial pressure (MAP) and heart rate (HR) were recorded beat to beat over 1 h in freely moving control ( n = 10) and acutely (1 day before study, n = 7) and chronically (2 wk before study, n = 10) sinoaortic-denervated (SAD) 12- to 14-wk-old male Sprague-Dawley rats. These beat-to-beat time series were successively low-pass filtered seven times and resampled at different time intervals from 0.1 to 6.4 s, allowing different timescales to be scanned. With the use of the Z coefficient, the statistical relationship was estimated for the associations of inverse MAP and HR variations when these inverse MAP and HR variations occurred simultaneously or were time shifted. In control rats and for timescales ≥0.4 s, the highest Zcoefficient(0.38) was obtained when MAP variations preceded inverse HR variations by one sampling interval. The baroreflex origin of this link was demonstrated by its disappearance after acute SAD. In conclusion, this method enabled spontaneous baroreflex episodes to be identified for unusually long timescales without limiting the study to fast, linear, stationary, or oscillating phenomena.

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