scholarly journals Biodegradable Polymersomes for the Delivery of Gemcitabine to Panc-1 Cells

2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
Nimil Sood ◽  
Walter T. Jenkins ◽  
Xiang-Yang Yang ◽  
Nikesh N. Shah ◽  
Joshua S. Katz ◽  
...  
Keyword(s):  
Controlled Release ◽  
Side Effects ◽  
Chemotherapeutic Agent ◽  
Therapeutic Index ◽  
Drug Bioavailability ◽  
Anticancer Chemotherapy ◽  
Poly Ethylene Oxide ◽  
Poly Ethylene ◽  
Poly Caprolactone

Traditional anticancer chemotherapy often displays toxic side effects, poor bioavailability, and a low therapeutic index. Targeting and controlled release of a chemotherapeutic agent can increase drug bioavailability, mitigate undesirable side effects, and increase the therapeutic index. Here we report a polymersome-based system to deliver gemcitabine to Panc-1 cells in vitro. The polymersomes were self-assembled from a biocompatible and completely biodegradable polymer, poly(ethylene oxide)-poly(caprolactone), PEO-PCL. We showed that we can encapsulate gemcitabine within stable 200 nm vesicles with a 10% loading efficiency. These vesicles displayed a controlled release of gemcitabine with 60% release after 2 days at physiological pH. Upon treatment of Panc-1 cells in vitro, vesicles were internalized as verified with fluorescently labeled polymersomes. Clonogenic assays to determine cell survival were performed by treating Panc-1 cells with varying concentrations of unencapsulated gemcitabine (FreeGem) and polymersome-encapsulated gemcitabine (PolyGem) for 48 hours. 1 μM PolyGem was equivalent in tumor cell toxicity to 1 μM FreeGem, with a one log cell kill observed. These studies suggest that further investigation on polymersome-based drug formulations is warranted for chemotherapy of pancreatic cancer.

Surface Modification of PEOT/PBT Membrane with Silk Fibroin Anchoring and its Potential Application in Artificial Salivary Gland Construct

2012 ◽  
Vol 538-541 ◽  
pp. 52-59
Author(s):  
Jie Zhu ◽  
Ming Shi Li ◽  
Li Qun Wang ◽  
Xiao Lin Zhu
Keyword(s):  
Salivary Gland ◽  
Silk Fibroin ◽  
Potential Application ◽  
Contact Angles ◽  
Water Contact ◽  
Polar Groups ◽  
Poly Ethylene Oxide ◽  
Poly Ethylene ◽  
Surface Modified

We reported the preparation of surface modified poly (ethylene oxide terephthalate) - poly (butylene terephthalate) membrane by the method of silk fibroin anchoring, namely SF/(PEOT/PBT). Its surface properties were characterized by contact angles and XPS and the biocompatibility of the composite membrane was further evaluated by human salivary epithelial cells (HSG cells) growth in vitro. Results revealed that SF/(PEOT/PBT) possessed the low water contact angle (48.0±3.0°) and immobilized a great amount of fibroin (fibroin surface coverage: 26.39 wt%), which attributed to the formation of polar groups such as hydrosulfide group, sulfonic group, carboxyl and carbonyl ones in the process of SO2 plasma treatment. HSG cells growth in vitro indicated that the silk fibroin anchoring could significantly enhance the biocompatibility of PEOT/PBT membrane, which suggested the potential application of fibroin anchoring PEOT/PBT for clinical HSG cells transplantation in the artificial salivary gland construct.

Encapsulation and In Vitro Controlled Release of Doxycycline in Temperature-Sensitive Hydrogel Composed of Polyethyleneglycol–polypeptide (L-Alanine-co-L-Aspartate)

2021 ◽  
Vol 49 ◽  
pp. 119-129
Author(s):  
Shamo Zokhrab Tapdiqov
Keyword(s):  
Controlled Release ◽  
Correlation Coefficients ◽  
Zero Order ◽  
Temperature Sensitive ◽  
Poly Ethylene Glycol ◽  
Drug Delivery Carrier ◽  
Ft Ir ◽  
Poly Ethylene ◽  
Potential Use

Doxycycline was loaded with synthesized micelles composed of methyl Poly (ethylene glycol-block-poly (L-alanine–co–L-aspartate), or mPEG–Ala–Asp, and then characterized as a drug delivery carrier. The synthesis of the temperature-sensitive mPEG–Ala–Asp block copolymer was carried out by two-step ring-opening polymerization: firstly, the mPEG reacts with L-alanine N-carboxylic anhydride, and secondly the resulting mPEG–Ala reacts with benzyl aspartate N-carboxylic anhydride. The molecular structure of the copolymers obtained was determined by FT-IR and NMR spectroscopy methods and the micelles were characterized by SEM, TEM and DLS, respectively. The controlled release of Dox from hydrogel in the presence of PBS (8 to 9% by weight) lasts 6 to 7 days exhibiting stable release rates. The drug release mechanisms were studied: Higuchi and zero order models. The results and correlation coefficients applied to the Higuchi and zero-order models. The findings show the potential use of mPEG–Ala–Asp as an effective depot matrix to deliver anthracycline class drugs.

scholarly journals Degradable Poly(ethylene oxide)-Like Plasma Polymer Films Used for the Controlled Release of Nisin

Polymers ◽  
2020 ◽  
Vol 12 (6) ◽  
pp. 1263 ◽  
Author(s):  
Jaroslav Kousal ◽  
Jana Sedlaříková ◽  
Zuzana Kolářová-Rašková ◽  
Zdeněk Krtouš ◽  
Liliana Kučerová ◽  
...  
Keyword(s):  
Controlled Release ◽  
Ethylene Oxide ◽  
Biologically Active ◽  
Process Conditions ◽  
Plasma Power ◽  
Permeability Study ◽  
Poly Ethylene Oxide ◽  
Poly Ethylene ◽  
Atr Spectroscopy ◽  
Permeation Properties

Poly(ethylene oxide) (PEO)-like thin films were successfully prepared by plasma-assisted vapor thermal deposition (PAVTD). PEO powders with a molar weight (Mw) between 1500 g/mol and 600,000 g/mol were used as bulk precursors. The effect of Mw on the structural and surface properties was analyzed for PEO films prepared at a lower plasma power. Fourier transform (FTIR-ATR) spectroscopy showed that the molecular structure was well preserved regardless of the Mw of the precursors. The stronger impact of the process conditions (the presence/absence of plasma) was proved. Molecular weight polydispersity, as well as wettability, increased in the samples prepared at 5 W. The influence of deposition plasma power (0–30 W) on solubility and permeation properties was evaluated for a bulk precursor of Mw 1500 g/mol. The rate of thickness loss after immersion in water was found to be tunable in this way, with the films prepared at the highest plasma power showing higher stability. The effect of plasma power deposition conditions was also shown during the permeability study. Prepared PEO films were used as a cover, and permeation layers for biologically active nisin molecule and a controlled release of this bacteriocin into water was achieved.

Surface Modification of Copolyether-Urethane Catheters with Poly(Ethylene Oxide)

1989 ◽  
Vol 12 (6) ◽  
pp. 390-394 ◽  
Author(s):  
E. Brinkman ◽  
A. Poot ◽  
T. Beugeling ◽  
L. Van Der Does ◽  
A. Bantjes
Keyword(s):  
Surface Modification ◽  
Ethylene Oxide ◽  
Blood Compatibility ◽  
Angle Measurements ◽  
Fold Reduction ◽  
Contact Angle Measurements ◽  
Platelet Deposition ◽  
Poly Ethylene Oxide ◽  
Poly Ethylene

Pellethane 2363 80A catheters were modified with poly(ethylene oxide) in order to improve their blood compatibility. Contact angle measurements showed that Pellethane 2363 80A surfaces had increased wettability after this modification. The results of in vitro blood compatibility tests showed that surface modification with poly(ethylene oxide) resulted in a five-fold reduction of platelet deposition. Activation of coagulation was not affected.

scholarly journals Hydrogel Nanofibers from Carboxymethyl Sago Pulp and Its Controlled Release Studies as a Methylene Blue Drug Carrier

Fibers ◽  
2019 ◽  
Vol 7 (6) ◽  
pp. 56 ◽  
Author(s):  
Nafeesa Mohd Kanafi ◽  
Norizah Abdul Rahman ◽  
Nurul Husna Rosdi ◽  
Hasliza Bahruji ◽  
Hasmerya Maarof
Keyword(s):  
Methylene Blue ◽  
Citric Acid ◽  
Controlled Release ◽  
Drug Carrier ◽  
Curing Temperature ◽  
Buffer Solution ◽  
Release Studies ◽  
Electrospinning Method ◽  
Poly Ethylene Oxide ◽  
Poly Ethylene

The potential use of carboxymethyl sago pulp (CMSP) extracted from sago waste for producing hydrogel nanofibers was investigated as a methylene blue drug carrier. Sago pulp was chemically modified via carboxymethylation reaction to form carboxymethyl sago pulp (CMSP) and subsequently used to produce nanofibers using the electrospinning method with the addition of poly(ethylene oxide) (PEO). The CMSP nanofibers were further treated with citric acid to form cross-linked hydrogel. Studies on the percentage of swelling following the variation of citric acid concentrations and curing temperature showed that 89.20 ± 0.42% of methylene blue (MB) was loaded onto CMSP hydrogel nanofibers with the percentage of swelling 4366 ± 975%. Meanwhile, methylene blue controlled release studies revealed that the diffusion of methylene blue was influenced by the pH of buffer solution with 19.44% of MB released at pH 7.34 within 48 h indicating the potential of CMSP hydrogel nanofibers to be used as a drug carrier for MB.

Sign in / Sign up

Export Citation Format

Share Document