scholarly journals Spectroscopic Investigations of Pentobarbital Interaction with Transthyretin

2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
Saqer M. Darwish ◽  
Jafar Ghithan ◽  
Musa M. Abuteir ◽  
Mariam Faroun ◽  
Mahmoud M. Abu-hadid
Keyword(s):  
Atomic Force Microscopy ◽  
Spectral Analysis ◽  
Fourier Transform ◽  
Secondary Structure ◽  
Thermal Denaturation ◽  
Force Microscopy ◽  
Atomic Force ◽  
Structure Changes ◽  
Intensity Changes ◽  
Amyloid Diseases

Transthyretin (TTR) aggregation has been characterized to be responsible for several amyloid diseases. Fourier transform infrared (FTIR) spectroscopy, fluorescence, and atomic force microscopy (AFM) are used to investigate secondary structure changes in transthyretin, induced upon thermal denaturation and interaction with pentobarbital. Spectral analysis revealed a strong static quenching of the intrinsic fluorescence of TTR by pentobarbital with a binding constant (K) estimated at2.092×103 M-1. Fourier self-deconvolution (FSD) technique is used to evaluates intensity changes in the spectra of the component bands in the amide I and amide II regions due to the changes in pentobarbital concentration in the protein complex. The increases of the relative intensities of the peaks at 1614 cm−1and 1507 cm−1are due to the increase of pentobarbital concentrations which is linked to the formation of oligomers in the protein.

Thermal denaturation of fibrinogen visualized by single-molecule atomic force microscopy

2018 ◽  
Vol 167 ◽  
pp. 370-376 ◽  
Author(s):  
Nikolay A. Barinov ◽  
Anna D. Protopopova ◽  
Evgeniy V. Dubrovin ◽  
Dmitry V. Klinov
Keyword(s):  
Atomic Force Microscopy ◽  
Single Molecule ◽  
Thermal Denaturation ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals Synthesis and design of laboratory device from proton conducting block copolymer membrane

BIBECHANA ◽  
2012 ◽  
Vol 9 ◽  
pp. 50-58
Author(s):  
Shankar P Khatiwada ◽  
Amar P Yadav ◽  
Werner Lebek ◽  
Jean Marc Saiter ◽  
Rameshwar Adhikari
Keyword(s):  
Electron Microscopy ◽  
Atomic Force Microscopy ◽  
Fourier Transform ◽  
Block Copolymer ◽  
Triblock Copolymer ◽  
Proton Conductance ◽  
Proton Conducting ◽  
Force Microscopy ◽  
Atomic Force ◽  
Laboratory Device

The butadiene units of polystyrene-block-polybutadiene-block-polystyrene (SBS) triblock copolymer were subjected to epoxidation and subsequent sulphonation in order to prepare proton conducting ionomer membrane. The products were characterized by different techniques such as atomic force microscopy, Fourier transform infrared (FTIR) spectroscopy and electron microscopy. A simple laboratory device was developed to carry out the tests on proton conductance ability of the prepared ionomer membrane. The experiments demonstrated that the prepared membrane excellently performed as proton conducting membrane. DOI: http://dx.doi.org/10.3126/bibechana.v9i0.7174 BIBECHANA 9 (2013) 50-58

scholarly journals Salmon Muscle Adherence to Polymer Coatings and Determination of Antibiotic Residues by Reversed-Phase High-Performance Liquid Chromatography Coupled to Selected Reaction Monitoring Mass Spectrometry, Atomic Force Microscopy, and Fourier Transform Infrared Spectroscopy

2015 ◽  
Vol 2015 ◽  
pp. 1-12 ◽  
Author(s):  
E. Zumelzu ◽  
M. J. Wehrhahn ◽  
O. Muñoz ◽  
F. Rull
Keyword(s):  
Mass Spectrometry ◽  
Atomic Force Microscopy ◽  
Fourier Transform ◽  
Infrared Spectroscopy ◽  
Fourier Transform Infrared Spectroscopy ◽  
Fourier Transform Infrared ◽  
Urea Solution ◽  
Antibiotic Residues ◽  
Force Microscopy ◽  
Atomic Force

The persistent adhesion of salmon muscle to food container walls after treatment with urea solution was observed. This work evaluated the diffusion of antibiotics from the salmon muscle to the polyethylene terephthalate (PET) coating protecting the electrolytic chromium coated steel (ECCS) plates. New aquaculture production systems employ antibiotics such as florfenicol, florfenicol amine, oxytetracycline, and erythromycin to control diseases. The introduction of antibiotics is a matter of concern regarding the effects on human health and biodiversity. It is important to determine their impact on the adhesion of postmortem salmon muscle to can walls and the surface and structural changes affecting the functionality of multilayers. This work characterized the changes occurring in the multilayer PET polymer and steel of containers by electron microscopy, 3D atomic force microscopy (3D-AFM), X-ray photoelectron spectroscopy (XPS), and Fourier transform infrared spectroscopy (FT-IR) analyses. A robust mass spectrometry methodology was employed to determine the presence of antibiotic residues. No evidence of antibiotics was observed on the protective coating in the range between 0.001 and 2.0 ng/mL; however, the presence of proteins, cholesterol, and alpha-carotene was detected. This in-depth profiling of the matrix-level elements is relevant for the use of adequate materials in the canning export industry.

scholarly journals Detection of Peptide-Lipid Interactions in Mixed Monolayers, Using Isotherms, Atomic Force Microscopy, and Fourier Transform Infrared Analyses

2000 ◽  
Vol 78 (2) ◽  
pp. 846-856 ◽  
Author(s):  
Véronique Vié ◽  
Nicole Van Mau ◽  
Laurent Chaloin ◽  
Eric Lesniewska ◽  
Christian Le Grimellec ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Fourier Transform ◽  
Fourier Transform Infrared ◽  
Mixed Monolayers ◽  
Force Microscopy ◽  
Lipid Interactions ◽  
Atomic Force
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