scholarly journals Effect of Glu-B3 Allelic Variation on Sodium Dodecyl Sulfate Sedimentation Volume in Common Wheat (Triticum aestivumL.)

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Hongqi Si ◽  
Manli Zhao ◽  
Fuxia He ◽  
Chuanxi Ma
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Allelic Variation ◽  
Sodium Dodecyl ◽  
Bread Making ◽  
Specific Pcr ◽  
Dodecyl Sulfate ◽  
Allele Specific ◽  
Sedimentation Volume ◽  
Improved Cultivars ◽  
Allele Specific Pcr

Sodium dodecyl sulfate (SDS) sedimentation volume has long been used to characterize wheat flours and meals with the aim of predicting processing and end-product qualities. In order to survey the influence of low-molecular-weight glutenin subunits (LMW-GSs) at Glu-B3 locus on wheat SDS sedimentation volume, a total of 283 wheat (Triticum aestivumL.) varieties including landraces and improved and introduced cultivars were analyzed using 10 allele-specific PCR markers at the Glu-B3 locus. The highest allele frequency observed in the tested varieties was Glu-B3i with 21.9% in all varieties, 21.1% in landraces, 25.5% in improved cultivars, and 12% in introduced cultivars. Glu-B3 locus represented 8.6% of the variance in wheat SDS sedimentation volume, and Glu-B3b, Glu-B3g, and Glu-B3h significantly heightened the SDS sedimentation volume, but Glu-B3a, Glu-B3c, and Glu-B3j significantly lowered the SDS sedimentation volume. For the bread-making quality, the most desirable alleles Glu-B3b and Glu-B3g become more and more popular and the least desirable alleles Glu-B3a and Glu-B3c got less and less in modern improved cultivars, suggesting that wheat grain quality in China has been significantly improved through breeding effort.

D-genome suppression of gliadin electrophoretic band 45 in hexaploid wheat and the effect upon quality

Genome ◽  
1990 ◽  
Vol 33 (3) ◽  
pp. 395-399 ◽  
Author(s):  
N. K. Howes ◽  
M. I. Kovacs ◽  
E. R. Kerber
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Hexaploid Wheat ◽  
Protein Gene ◽  
Sodium Dodecyl ◽  
D Genome ◽  
Electrophoretic Band ◽  
Endosperm Protein ◽  
Dodecyl Sulfate ◽  
Sedimentation Volume ◽  
The Cross

The hexaploid wheat 'Prelude' (2n = 42 = AABBDD) contains the endosperm protein electrophoretic band 40, but not band 45, while the extracted tetraploid 'Tetraprelude' (2n = 28 = AABB) contains electrophoretic band 45 but not band 40. Moreover, F3 progeny from the cross between backcross lines of 'Prelude' and 'Marquis' segregated as if both of these proteins were controlled by alleles of one gene, or by two very closely linked genes. Examination of the F3 seed from the cross 'Prelude' × 'Canthatch' 7D nullisomic showed that band 45 was not expressed in any of the 334 F2 families, including nine nullisomics. Together, these results indicate that band 45 endosperm protein is not suppressed by a gene on chromosome 7D. Furthermore, removal of the D genome from 'Prelude' resulted in increased kernel hardness and increased gluten elastic strength but a decrease in sodium dodecyl sulfate sedimentation volume. However, the sodium dodecyl sulfate sedimentation volume of 'Tetraprelude', was the highest among the tetraploid cultivars evaluated.Key words: wheat, sodium dodecyl sulfate electrophoresis, endosperm protein, gene expression.

EVALUATION OF CONVENTIONAL AND PROLONGED-SWELLING SODIUM DODECYL SULFATE SEDIMENTATION TESTS FOR THE PREDICTION OF BREAD WHEAT QUALITY UNDER HEAT STRESS CONDITIONS

2009 ◽  
Vol 45 (2) ◽  
pp. 165-175
Author(s):  
IZZAT S. A. TAHIR ◽  
NOBORU NAKATA ◽  
ABDELBAGI M. ALI ◽  
ABU SEFYAN I. SAAD ◽  
WATARU TSUJI
Keyword(s):  
Heat Stress ◽  
Sodium Dodecyl Sulfate ◽  
Bread Wheat ◽  
Sodium Dodecyl ◽  
Quality Attributes ◽  
Stress Conditions ◽  
Peak Time ◽  
Bread Making ◽  
Dodecyl Sulfate ◽  
End Use

SUMMARYIn non-traditional, heat-stressed environments, wheat breeding programmes were mainly concerned with the introduction and adaptation of high-yielding, heat-tolerant cultivars regardless of the end-use quality. For the recently developed quality-oriented markets, new cultivars should combine the above-mentioned traits with good grain quality to improve economic feasibility of wheat production in these environments. This study aimed to examine the suitability of the conventional sodium dodecyl sulfate sedimentation (SDSS) test for predicting bread-making quality and to evaluate the effectiveness of a prolonged-swelling SDSS test in improving the predictability of end-use quality under heat stress conditions. Conventional and prolonged-swelling SDSS volumes were measured from whole meal of 15 bread wheat genotypes grown for two seasons under two sowing conditions at Gezira Research Farm, Wad Medani, Sudan. Results of correlations of SDSS volumes with total and insoluble protein contents, mixograph peak height and mixograph descending slope indicated the suitability of the SDSS test in predicting bread-making quality under heat-stress irrigated conditions. However, the absence of significant correlations with some quality attributes, such as mixograph peak time and mixograph curve width, demonstrated the non-exclusiveness of the SDSS test for predicting all bread-making quality attributes. The prolonged-swelling SDSS tests did not improve identification of differences among genotypes over the conventional test despite similarly predicting some quality attributes and showing relatively small increases in the correlation coefficient magnitudes with others. SDSS after 10 min from settlement (SDSS10) showed strong correlations with all other SDSS volumes at various times and with most of quality attributes. This suggested that SDSS10 could be used for evaluation of bread-making quality in early generations of the breeding programme in the hot irrigated conditions of Sudan and similar environments.

scholarly journals Identification and Validation of Stable Quantitative Trait Loci for SDS-Sedimentation Volume in Common Wheat (Triticum aestivum L.)

2021 ◽  
Vol 12 ◽  
Author(s):  
Shuai Tian ◽  
Minghu Zhang ◽  
Jinghui Li ◽  
Shaozhe Wen ◽  
Chan Bi ◽  
...  
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Common Wheat ◽  
Quantitative Trait ◽  
Positional Cloning ◽  
Sodium Dodecyl ◽  
Snp Markers ◽  
Reference Sequence ◽  
Phenotypic Variance ◽  
Dodecyl Sulfate ◽  
Sedimentation Volume

Sodium dodecyl sulfate-sedimentation volume is an important index to evaluate the gluten strength of common wheat and is closely related to baking quality. In this study, a total of 15 quantitative trait locus (QTL) for sodium dodecyl sulfate (SDS)-sedimentation volume (SSV) were identified by using a high-density genetic map including 2,474 single-nucleotide polymorphism (SNP) markers, which was constructed with a doubled haploid (DH) population derived from the cross between Non-gda3753 (ND3753) and Liangxing99 (LX99). Importantly, four environmentally stable QTLs were detected on chromosomes 1A, 2D, and 5D, respectively. Among them, the one with the largest effect was identified on chromosome 1A (designated as QSsv.cau-1A.1) explaining up to 39.67% of the phenotypic variance. Subsequently, QSsv.cau-1A.1 was dissected into two QTLs named as QSsv.cau-1A.1.1 and QSsv.cau-1A.1.2 by saturating the genetic linkage map of the chromosome 1A. Interestedly, favorable alleles of these two loci were from different parents. Due to the favorable allele of QSsv.cau-1A.1.1 was from the high-value parents ND3753 and revealed higher genetic effect, which explained 25.07% of the phenotypic variation, mapping of this locus was conducted by using BC3F1 and BC3F2 populations. By comparing the CS reference sequence, the physical interval of QSsv.cau-1A.1.1 was delimited into 14.9 Mb, with 89 putative high-confidence annotated genes. SSVs of different recombinants between QSsv.cau-1A.1.1 and QSsv.cau-1A.1 detected from DH and BC3F2 populations showed that these two loci had an obvious additive effect, of which the combination of two favorable loci had the high SSV, whereas recombinants with unfavorable loci had the lowest. These results provide further insight into the genetic basis of SSV and QSsv.cau-1A.1.1 will be an ideal target for positional cloning and wheat breeding programs.

Genomic Evaluation of Sunflower Broomrape (Orobanche Cumana) Germplasm by KASP Assay

Helia ◽  
2018 ◽  
Vol 41 (68) ◽  
pp. 57-72
Author(s):  
Nilay Yonet ◽  
Yıldız Aydin ◽  
Goksel Evci ◽  
Ahu Altinkut Uncuoglu
Keyword(s):  
Agricultural Research ◽  
Allelic Variation ◽  
Allelic Discrimination ◽  
Orobanche Cumana ◽  
Specific Pcr ◽  
Kasp Assay ◽  
Allele Specific ◽  
Sunflower Broomrape ◽  
Allele Specific Pcr ◽  
Thrace Region

AbstractOrobanche cumana Wallr. is a holoparasitic plant for only sunflower, hence it is called as sunflower broomrape. Yield loss created by O. cumana which is generally 50 % can reach to 100 %. In this study, it was planned to perform molecular characterization of O. cumana germplasm as nine locations of Thrace region obtained from Trakya Agricultural Research Institute by using Single Nucleotide Polymorphism (SNP) markers, widely used in plant breeding programs, in Competitive Allele Specific PCR (KASP) assay which is a fluorescent tagged allele specific PCR method based, economic, reliable and easily repeatable genotyping technology. Databases and literature were scanned to spot variations on O. cumana genome which is not known clearly. So far, four SSR (Simple Sequence Repeat) marker (Ocum-197, Ocum-006, Ocum-023 and Ocum-151) regions showing polymorphic pattern were used for searching possible SNPs. Primer pairs were designed for amplification of the regions possibly having SNPs and PCR amplifications with these primer pairs were performed and 1 candidate deletion was detected on the amplicon which was amplified by Ocum-197 SSR marker. Following, the deletion was converted to KASP primers and KASP assay was performed. The deletion marker, Del-197, has grouped the samples from nine locations in the resulting allelic discrimination plot and infestation was performed according to this grouping, As a conclusion, Del-197 is considered as a selective marker for the ability to rapidly assay allelic variation at DNA markers for O. cumana populations that have effects on infestation results were evaluated as races, F, G, H and I in Thrace region.

Simple and Rapid Detection of Factor V Leiden by Allele-specific PCR Amplification

1996 ◽  
Vol 75 (05) ◽  
pp. 757-759 ◽  
Author(s):  
Rainer Blasczyk ◽  
Markus Ritter ◽  
Christian Thiede ◽  
Jenny Wehling ◽  
Günter Hintz ◽  
...  
Keyword(s):  
Direct Sequencing ◽  
Activated Protein C ◽  
Factor V Leiden ◽  
Pcr Amplification ◽  
Factor V ◽  
Specific Pcr ◽  
Pcr Rflp ◽  
Allele Specific ◽  
Specific Amplification ◽  
Allele Specific Pcr

SummaryResistance to activated protein C is the most common hereditary cause for thrombosis and significantly linked to factor V Leiden. In this study, primers were designed to identify the factor V mutation by allele-specific PCR amplification. 126 patients with thromboembolic events were analysed using this technique, PCR-RFLP and direct sequencing. The concordance between these techniques was 100%. In 27 patients a heterozygous factor VGln506 mutation was detected, whereas one patient with recurrent thromboembolism was homozygous for the point mutation. Due to its time- and cost-saving features allele-specific amplification should be considered for screening of factor VGln506.

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