scholarly journals Simultaneous Determination of Glycyrrhizin and 15 Flavonoids in Licorice and Blood by High Performance Liquid Chromatography with Ultraviolet Detector

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Yongqian Zhang ◽  
Jin Cao ◽  
Yingping Wang ◽  
Shengyuan Xiao
Keyword(s):  
High Performance Liquid Chromatography ◽  
High Performance ◽  
Dynamic Range ◽  
High Dynamic Range ◽  
Rat Plasma ◽  
Simple Method ◽  
Ultraviolet Detector ◽  
Different Types ◽  
High Dynamic

Licorice is the most frequently used herb in traditional Chinese medicine (TCM) with versatile functions. It is also a popular natural dietary supplement. While the dosages is very important for there are some side effects caused by licorice. The composition of licorice, its products should be well determined thereof. A simple method for simultaneous determining sixteen compounds in vary high dynamic range of content has been established. This method based on the detection at the characteristic ultraviolet spectra of different types of compounds in licorice. Glycyrrhizin and fifteen flavonoids were well measured. All of these compounds can be precisely quantified at their characteristic wavelengths. This method has been successfully applied to the analyses of different licorices, Sini Tang decoction, and rat plasma after oral administration of Sini Tang decoction. These compounds were found to be over 3000 times in content (from 0.01 μg/g to 34.5 μg/g) in some samples.

Simultaneous separation and preconcentration of phosalone and chlorpyrifos in fresh vegetables using ultrasound-assisted dispersive liquid–liquid microextraction and high performance liquid chromatography

2016 ◽  
Vol 8 (18) ◽  
pp. 3795-3801 ◽  
Author(s):  
Monireh Majlesi ◽  
Mohamadreza Massoudinejad ◽  
Fateh Hosainzadeh ◽  
Nazir Fattahi
Keyword(s):  
High Performance Liquid Chromatography ◽  
High Performance ◽  
Simple Method ◽  
Fresh Vegetables ◽  
Separation And Preconcentration ◽  
Simultaneous Separation ◽  
Ultrasound Assisted ◽  
Dispersive Liquid Liquid Microextraction ◽  
Liquid Microextraction

UA-DLLME-SFO combined with HPLC-UV is a fast and simple method for the determination of pesticides and herbicides in fresh vegetables.

Establishment of an HPLC Method for Determination of Coumarin-3-Carboxylic Acid Analogues in Rat Plasma and a Preliminary Study on Their Pharmacokinetics

2021 ◽  
Author(s):  
Yan Xiong ◽  
Yong-Hong Liu ◽  
Jian-Sha Li ◽  
Yu-Ying Zhang ◽  
Jing Zhang ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Carboxylic Acid ◽  
High Performance ◽  
Rat Plasma ◽  
Hplc Method ◽  
Array Detector ◽  
Pharmacokinetic Parameters ◽  
Preliminary Study

Abstract A simple high performance liquid chromatography (HPLC) method was developed and validated for the determination of coumarin-3-carboxylic acid analogues (C3AA) in rat plasma and a preliminary study on pharmacokinetics. Ferulic acid (FA) was used as the internal standard substance, and coumarin-3-carboxylic acid (C3A) was used as a substitute for quantitative C3AA. After protein precipitation with methanol, the satisfactory separation was achieved on an ODS2 column when the temperature was maintained at 30 ± 2°C. The correlation coefficient r in the C3A linear equation is equal to 0.9990. Pharmacokinetic parameters for t1/2, Tmax, Cmax, area under the curve (AUC)0-t, average residence time (MRT), apparent volume of distribution (V z/F) and clearance (Cl/F) were 1.89 ± 0.03 h, 0.39 ± 0.14 h, 1.81 ± 0.10 g· mL−1 ·h, 7.88 ± 0.24 g·mL−1·h, 3.23 ± 0.14 h, 0.43 ± 0.03 (mg·kg−1)·(g·mL−1)−1·h−1, respectively. The high performance liquid chromatography-photo diode array detector (HPLC-PDA) method established in this study can be used to separate and determine the content of C3AA in plasma of rats after 60% ethanol extraction by gavage. The plasma concentration-time curve and pharmacokinetic parameters reflect the absorption of C3AA in rat blood after oral administration to some extent.

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