scholarly journals Cytotoxic Effect of Ethanol Extract of Microalga,Chaetoceros calcitrans, and Its Mechanisms in Inducing Apoptosis in Human Breast Cancer Cell Line

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Siyamak Ebrahimi Nigjeh ◽  
Fatimah Md Yusoff ◽  
Noorjahan Banu Mohamed Alitheen ◽  
Mehdi Rasoli ◽  
Yeap Swee Keong ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Cycle ◽  
Cytotoxic Effect ◽  
Cancer Cell Line ◽  
Ethanol Extract ◽  
Cancer Drug ◽  
Human Breast ◽  
Ic50 Value ◽  
Chaetoceros Calcitrans ◽  
Mcf 7

Marine microalgae have been prominently featured in cancer research. Here, we examined cytotoxic effect and apoptosis mechanism of crude ethanol extracts of an indigenous microalga,Chaetoceros calcitrans(UPMAAHU10) on human breast cell lines. MCF-7 was more sensitive than MCF-10A with IC50 value of3.00±0.65, whilst the IC50 value of Tamoxifen against MCF-7 was12.00±0.52 μg/mL after 24 hour incubation. Based on Annexin V/Propidium iodide and cell cycle flow cytometry analysis, it was found that inhibition of cell growth by EEC on MCF-7 cells was through the induction of apoptosis without cell cycle arrest. The apoptotic cells at subG0/G1 phase in treated MCF-7 cells at 48 and 72 hours showed 34 and 16 folds increased compared to extract treated MCF-10A cells which showed only 6 and 7 folds increased at the same time points, respectively. Based on GeXP study, EEC induced apoptosis on MCF-7 cells via modulation of CDK2, MDM2, p21Cip1, Cyclin A2, Bax and Bcl-2. The EEC treated MCF-7 cells also showed an increase in Bax/Bcl-2 ratio that in turn activated the caspase-dependent pathways by activating caspase 7. Thus, marine microalga,Chaetoceros calcitransmay be considered a good candidate to be developed as a new anti-breast cancer drug.

scholarly journals In Vitro Cytotoxicity of the Synthesized Gallic Acid Derivatives (N-Alkyl Gallamide) Against Breast MCF-7 Cancer Cells

2018 ◽  
Vol 34 (5) ◽  
pp. 2268-2272
Author(s):  
Maurin Marcelia ◽  
Ade Arsianti ◽  
Jilly Octaria Tagore Chan ◽  
Stevano Julio Wijoyo ◽  
Fadilah Fadilah ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Gallic Acid ◽  
Cytotoxic Effect ◽  
Linear Regression Analysis ◽  
Cancer Cell Line ◽  
In Vitro Cytotoxicity ◽  
Tert Butyl ◽  
Ic50 Value ◽  
Mcf 7

Gallic acid is a phenolic compound distributed in plants and fruits which has been reported to have cytotoxic effect on MCF-7 breast cancer cell line. In this research, we investigated in vitro cytotoxic effect of six synthesized compounds of gallic acid derivatives (N-alkyl gallamide), namely N-methyl gallamide (2); N-ethyl gallamide (3); N-butyl gallamide (4); N-sec-butyl gallamide (5); N-tert-butyl gallamide (6) and N-hexyl gallamide (7) against breast MCF-7 cells by MTT assay. Linear regression analysis is utilized to analyze data to regenerate IC50 value. The results will be compared with gallic acid as an original compound and doxorubicin as a positive control.Among six synthesized compounds, N-tert-butyl gallamide (6) with IC50 value of 2.1 µg/mL, and N-hexyl gallamide (7) with IC50 value of 3.5µg/mL,showed the stronger cytotoxicity against breast MCF-7 cells compared to gallic acid and doxorubicin. Thus,N-tert-butyl gallamide (6) and N-hexyl gallamide (7) are potential to be further developed as a promising anti-breast cancer agents.

scholarly journals Inhibitory effect of isoamericanol A from Jatropha curcas seeds on the growth of MCF-7 human breast cancer cell line by G2/M cell cycle arrest

Heliyon ◽  
2016 ◽  
Vol 2 (1) ◽  
pp. e00055 ◽  
Author(s):  
Ayako Katagi ◽  
Li Sui ◽  
Kazuyo Kamitori ◽  
Toshisada Suzuki ◽  
Takeshi Katayama ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Cycle ◽  
Human Breast Cancer ◽  
Cancer Cell Line ◽  
Inhibitory Effect ◽  
Human Breast Cancer Cell ◽  
Human Breast ◽  
M Cell ◽  
Cycle Arrest ◽  
Mcf 7
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