scholarly journals Expression and Clinical Significance of Livin Protein in Hepatocellular Carcinoma

2013 ◽  
Vol 35 ◽  
pp. 489-496 ◽  
Author(s):  
Hua Guo ◽  
Ying-tang Gao ◽  
Qin Zhang ◽  
Li Jing ◽  
Tong Liu ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Survival Rate ◽  
Protein Expression ◽  
Blood Plasma ◽  
Clinical Significance ◽  
Blood Donors ◽  
Hepatic Cirrhosis ◽  
Significant Difference ◽  
Before And After

In this study, the two-step PV method of immunohistochemistry was used to determine livin protein expression in HCC tissues, pericarcinoma tissues, hepatitis/hepatic cirrhosis tissues, and normal hepatic tissues, and livin protein expression was detected in the blood plasma of patients with HCC before and after surgery, subjects with hepatic cirrhosis and hepatitis, and healthy blood donors using ELISA. Livin protein expression was significantly higher in HCC tissues than that in normal hepatic tissues and hepatitis/hepatic cirrhosis tissues, with no significant difference between HCC tissues and pericarcinoma tissues. The HCC patients with positive livin protein expression had a significantly higher survival rate than those with negative livin protein expression. Livin protein expression was significantly higher in the blood plasma of patients with HCC before and after surgery and in patients with hepatic cirrhosis and hepatitis than that in healthy blood donors, whereas livin protein expression in the blood plasma of patients with HCC was not significantly different from that of patients with hepatic cirrhosis and hepatitis. Livin protein expression in HCC tissues did not correlate with that in the blood plasma of the same HCC patients. Livin protein expression may be a potential, effective indicator for assessing prognosis in patients with HCC.

Therapeutic Efficacy of Transcatheter Arterial Chemoembolization as Compared With Hepatic Resection in Hepatocellular Carcinoma Patients With Compensated Liver Function in a Hepatitis B Virus–Endemic Area: A Prospective Cohort Study

2002 ◽  
Vol 20 (22) ◽  
pp. 4459-4465 ◽  
Author(s):  
Hyo-Suk Lee ◽  
Kang Mo Kim ◽  
Jung-Hwan Yoon ◽  
Tae-Rim Lee ◽  
Kyung Suk Suh ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Survival Rate ◽  
Liver Function ◽  
Hepatic Resection ◽  
Transcatheter Arterial Chemoembolization ◽  
Survival Rates ◽  
Tumor Stage ◽  
Significant Difference ◽  
Tace Group ◽  
Arterial Chemoembolization

PURPOSE: Identifying a special subgroup of hepatocellular carcinoma (HCC) patients who may benefit from transcatheter arterial chemoembolization (TACE) when compared with the standard treatment of hepatic resection (HR) warrants research in Asian countries. PATIENTS AND METHODS: From January 1993 to December 1994, 182 patients with operable HCC (Child-Pugh class A and International Union Against Cancer [UICC] stage T1-3N0M0) were enrolled. After initial TACE and lipiodol computed tomography, 91 received HR and 91, who refused the operation, received repeated sessions of TACE. After stratification according to the tumor stage (UICC and Cancer of the Liver Italian Program [CLIP]) and lipiodol retention pattern, the survival rates of the two treatment groups were compared. The median follow-up period was 83 months. RESULTS: As of December 31, 2000, 48 patients who underwent HR and 68 patients who underwent TACE had died. In a subgroup analysis according to tumor stage, the HR group survival rate was significantly higher than the TACE group in both UICC T1-2N0M0 (P = .0058) and CLIP 0 (P = .0027) subgroups. However, there was no significant difference in either UICC T3N0M0 (P = .7512) or CLIP 1-2 (P = .5366) subgroups. Even in patients with UICC T1-2N0M0 HCC, when lipiodol was compactly retained, the survival rate of the HR group was comparable to that of the TACE group (P = .0596). CONCLUSION: TACE proved to be as effective as HR in the subpopulations with UICC T3N0M0 or CLIP 1-2 HCC and adequate liver function, and even with UICC T1-2N0M0 HCC when lipiodol was compactly retained in the tumor. In such cases, the choice of treatment modality between TACE and HR may be left to the patient’s preference.

Comparison of Gravity Collection Versus Suction Collection for Transfusion Purposes in Dogs

2013 ◽  
Vol 49 (5) ◽  
pp. 301-307 ◽  
Author(s):  
Bérénice Conversy ◽  
Marie-Claude Blais ◽  
Lisa Carioto ◽  
Julie Beaudoin
Keyword(s):  
Blood Donors ◽  
Blood Product ◽  
Blood Donation ◽  
Cost Effective ◽  
Clinical Parameters ◽  
Cost Effective Method ◽  
Significant Difference ◽  
Before And After ◽  
Collection Methods ◽  
Time Point

Blood donation is an essential step in transfusion medicine that must take into account the donor’s welfare, collection effectiveness, and blood product quality. This prospective study enrolled 13 canine blood donors, each subjected to both gravity and suction collection methods, in a randomized order. Clinical parameters, including heart rate (HR), respiratory rate (RR), systolic blood pressure (SBP), and rectal temperature (RT), were evaluated at four time points, including when the donor was on the floor and on the collection table, and before and after blood donation. The number of times the donor and needle required repositioning, the duration of the donation, the noise created by the apparatus, and the presence of a hematoma were evaluated. The weight, index of hemolysis, and hematocrit of each unit of blood were recorded. There was no significant difference between collection methods for either the clinical parameters at each time point or the prevalence of hematoma formation, the frequency of needle repositioning, the hemolysis index, or hematocrit. Collection by suction was noisier (P < 0.0001), faster (P = 0.004), and associated with significantly less donor repositioning (P = 0.007). Suction appears to be a safe and cost-effective method that should be considered to optimize blood donation.

Chondroitin Sulfate Iron Colloid as MR Contrast Agent in Differentiation between Hepatocellular Carcinoma and Adenomatous Hyperplasia

1993 ◽  
Vol 34 (3) ◽  
pp. 226-229 ◽  
Author(s):  
Y. Suto ◽  
T. Kato ◽  
T. Matsuo ◽  
M. Kamba ◽  
Y. Shimatani ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Chondroitin Sulfate ◽  
The Other ◽  
Adenomatous Hyperplasia ◽  
Cellular Functions ◽  
Visual Evaluation ◽  
Significant Difference ◽  
Before And After ◽  
T1 And T2 ◽  
T2 Weighted Images

Using a 1.5 T MR imaging unit, T1- and T2-weighted images were obtained before and after i.v. administration of chondroitin sulfate iron colloid (CSIC) in order to differentiate hepatocellular carcinoma (n = 20) from adenomatous hyperplasia without atypia (n = 16). Differentiation was made from the tumor-liver contrast to noise ratio (CNR) and visual evaluation of the nodule, with reference to signal intensity relative to that of the surrounding liver. The CNR of adenomatous hyperplasia was on T1-weighted images significantly decreased after CSIC administration (p < 0.01). On T2-weighted images, there was no significant difference in CNR after CSIC administration. On the other hand, the CNR of hepatocellular carcinoma was significantly increased after CSIC administration on both T1- and T2-weighted images (p < 0.01). CSIC reflects intratumor reticuloendothelial cellular functions, and is therefore useful in differentiating hepatocellular carcinoma from adenomatous hyperplasia without atypia.

scholarly journals Overview and Clinical Significance of Multiple Mutations in Individual Genes in Hepatocellular Carcinoma

2021 ◽  
Author(s):  
Taisuke Imamura ◽  
Yukiyasu Okamura ◽  
Keiichi Ohshima ◽  
Katsuhiko Uesaka ◽  
Teiichi Sugiura ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Clinical Significance ◽  
Independent Predictor ◽  
Single Mutation ◽  
Recurrence Free Survival ◽  
Wild Type ◽  
Free Survival ◽  
Significant Difference ◽  
And Function ◽  
Worse Prognosis

Abstract BackgroundMultiple mutations (MMs) within individual oncogenes have been newly characterized as a mechanism for promotion of carcinogenesis. We investigated the spectra of the MMs and the clinical significance in hepatocellular carcinoma (HCC). MethodsWhole-exome sequencing and gene expression profiling were performed in 223 surgically resected HCCs. ResultsMMs within individual genes was identified in 178 samples (79.8%, MMs tumors). All the remaining samples carried single mutation (20.2%, SM tumors). Mutations identified as MMs show different mutational patterns with higher functional impact compared with mutations identified as SM. Recurrence-free survival was significantly worse in the group with MMs tumors than the group with SM tumors (P = 0.012). MMs tumor was identified as an independent predictor for worse prognosis (hazard ratio, 1.72; 95%, P = 0.045). MMs were observed particularly in MUC16 (15% of samples with at least one mutation in the gene) and CTNNB1 (14%). Although there was no significant difference in MUC16 mRNA expression between MUC16 wild-type and MUC16 SM tumors, the expression in MUC16 MMs tumors was significantly enhanced compared with MUC16 SM tumors (P < 0.001). MMs in MUC16 were associated with viral hepatitis, higher tumor markers and vascular invasion. Recurrence-free survival was significantly worse in the MUC16 MMs group than the MUC16 SM group (P = 0.022); no significant difference was observed between the MUC16 SM group and MUC16 wild-type group (P = 0.324).ConclusionsMMs are relatively common driver events that selectively occur in specific oncogenes and function in tumor-promoting activity.

scholarly journals Effects of In-Hospital Physical Therapy on Activities of Daily Living in Patients with Hepatocellular Carcinoma

2020 ◽  
Vol 17 (23) ◽  
pp. 9098
Author(s):  
Hayato Narao ◽  
Keisuke Hirota ◽  
Shunji Koya ◽  
Manabu Tomita ◽  
Yuta Manako ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Physical Therapy ◽  
Activities Of Daily Living ◽  
Cancer Treatment ◽  
Daily Living ◽  
Functional Independence Measure ◽  
Functional Independence ◽  
Knee Extension ◽  
Significant Difference ◽  
Before And After

Activities of daily living (ADL) are frequently impaired in patients with hepatocellular carcinoma (HCC). In this retrospective study, we aimed to investigate the effects of physical therapy on ADLs in patients with HCC during hospitalization for cancer treatment. Nineteen patients with HCC were enrolled. During hospitalization, patients performed a combination of resistance training, stretching, and aerobic exercise (20–60 min/day). ADLs were assessed using the functional independence measure (FIM). Changes in FIM were evaluated by before–after analysis. No significant difference was seen in Child–Pugh class before and after physical therapy. The bilateral knee extension strength and chair stand test were significantly increased after physical therapy compared with before physical therapy (p = 0.001 and p = 0.008, respectively). The total FIM score was significantly increased after physical therapy compared with that before physical therapy (p = 0.0156). Among the 18 indexes of FIM, the stairs index was significantly improved after physical therapy compared with that before physical therapy (5.9 vs. 6.4 points, p = 0.0241). We demonstrated that physical therapy improved muscle strength without worsening liver function. Furthermore, physical therapy improved FIM, especially in the stairs index, in patients with HCC. Thus, physical therapy may be beneficial in patients with HCC during cancer treatment.

scholarly journals CLINICAL SIGNIFICANCE OF PLASMA MIR-21, MIR-141, MIR-29C, AND MIR-BART7 IN PATIENTS WITH LOCALLY ADVANCED NASOPHARYNGEAL CANCER AND THEIR ALTERATIONS AFTER CHEMORADIATION THERAPY

2019 ◽  
Vol 1 (02) ◽  
pp. 45-49
Author(s):  
Cita Herawati
Keyword(s):  
Treatment Response ◽  
Clinical Significance ◽  
Locally Advanced ◽  
Nasopharyngeal Cancer ◽  
Normal Subjects ◽  
Complete Response ◽  
Clinicopathological Characteristics ◽  
Significant Difference ◽  
Before And After ◽  
Polymerase Chain

Introduction: Plasma microRNAs (miRNAs) are biological markers that have been extensively studied in cancer, including nasopharyngeal cancer (NPC). The clinical significance of miRNA in NPC patients in Indonesia has never been studied. Objective: This study was aimed to know the expression of plasma miRNAs in NPC patients (miR-21, miR-29c, miR-141dan miR-BART7) and their relationship with clinicopathological characteristics and treatment response. Method: This was a cohort, longitudinal study among locally advanced NPC patients (stage IIB-IVB) in Dharmais Cancer Hospital, Jakarta. miRNA expression was assayed using quantitative real-time polymerase chain reaction (qRT-PCR) technique. Four miRNAs were evaluated, i.e., miR-21, miR-29c, miR-141, and EBV-miR-BART7. The results were normalized against a reference gene, miR-16. Result: A total of 52 patients and 10 normal subjects were enrolled in this study; 17 of them completed treatment. Patients’ mean age was 45.1+12.53 (14-68) years. The ratio between men and women was 3:1. MiR-21 and miR-29c could be detected in all subjects; miR-141 was detected in 22 (42.3%) and EBV-miR-BART7 in 26 (50%) subjects. There was no significant difference between miR-21 or miR-29c expression between before and after therapy. However, miR-21 expressions tend to decrease in a patient with complete response (CR) (4.13+3.65 vs. 2.74+3.23; p=0.650) and tend to increase in patients with partial response (PR) (3.00+5.86 vs. 8.77+8.43; p=0.465). There was no difference of miR-29c expression between CR and PR patients. Conclusion: Our study shows that not all miRNA can be detected in the plasma of NPC patients. Levels of miRNA expressions in these locally advanced patients are similar. Expression of miR-21 is potentially used as a biomarker of evaluating treatment response in NPC patients.

scholarly journals Comparative Analysis of Hepatitis B Virus Infections in Blood Donors Born Before and After the I Mplementation of Universal HBV Vaccination in Southern China

2020 ◽  
Author(s):  
Xianlin Ye ◽  
Tong Li ◽  
Yi Li ◽  
Jinfeng Zeng ◽  
Ran Li ◽  
...  
Keyword(s):  
Hepatitis B ◽  
Blood Donors ◽  
Vaccination Program ◽  
Escape Mutation ◽  
Hepatitis B Vaccination ◽  
Infected Blood ◽  
Hbv Vaccination ◽  
Significant Difference ◽  
Before And After ◽  
Genotype C

Abstract BackgroundNeonatal hepatitis B vaccination program at birth has been implemented nationwide since 1992 in China, which may impact HBV safety in blood donations actively. The true prevalence of HBV, HBsAg, and OBI between vaccinated blood donors and non-vaccinated blood donors should be explored. Study design and methodsThe samples of blood donors were collected and detected for serologic markers of HBV in the Shenzhen Blood Center between Feb 2016 and Jun 2016. The discrepant results were tested with commercial electrochemiluminescence immunoassay (ELCI), alternative MPX ID NAT, nested PCR, sequencing, and a quantitative real-time polymerase chain reaction (PCR) assay. HBsAg and anti-HBs were quantified. The serological and molecular characteristics of HBV infected blood donors were analyzed, and the effects on blood safety for donors born before and after the implementation of universal HBV vaccination were compared. ResultsTotal of 242 reactive by NAT and/or HBsAg ELISA samples from 26318 candidate donors, 192 (0.73%, [95%CI, 0.63-0.84]) HBV+, 131 (0.49%, [95%CI, 0.43-0.59]) HBsAg+, 58 (0.22%, [95%CI,0.17-0.28]) OBI were confirmed respectively. The HBV+ rate in vaccinated donors is lower than in non-vaccinated donors (P<0.05). The HBsAg titers of vaccinated infected blood donors are much higher than non vaccinated infected blood donors. The OBI yield rates in the vaccinated blood donors were 0.11% (7/6422), and significantly lower than 0.26% (51/19898) in the non vaccinated blood donors (P<0.05). 102/124 (82.3%) samples are genotype B, 22/124 (17.7%) are genotype C total. There is no significant difference in the distribution of genotype in the non vaccinated blood donors (B/C,86/17) and the vaccinated blood donors (B/C,23/6) (P>0.05). High frequency of vaccine escape mutation M133L (32.4%) and E164G in S region of genotype B strains and substitution L175S (40.9%) related to vaccine escape in S region of genotype C strains identified.ConclusionThe universal HBV vaccination program markedly reduces the risk of HBV infection in blood donors, and provides a significant guarantee for the safety of blood transfusion. Several important mutations detected related vaccine escape and notable mutations needed further investigated.

scholarly journals Clinical Significance of AFP and PIVKA-II Responses for Monitoring Treatment Outcomes and Predicting Prognosis in Patients with Hepatocellular Carcinoma

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Hana Park ◽  
Jun Yong Park
Keyword(s):  
Hepatocellular Carcinoma ◽  
Treatment Outcomes ◽  
Clinical Significance ◽  
Vitamin K ◽  
Treatment Modality ◽  
Good Deal ◽  
Inclusion Criteria ◽  
Before And After ◽  
Prediction Of Prognosis ◽  
Monitoring Treatment

Aim. Recently, the utility of tumor markers in the hepatocellular carcinoma (HCC) field has received a good deal of attention. Here, we review and summarize the results of studies on the roles played by theα-fetoprotein (AFP) and prothrombin induced by the absence of vitamin K or antagonist-II (PIVKA-II) responses in terms of the monitoring of outcomes and prediction of prognosis after various HCC treatments.Methods. Studies lodged in PUBMED and that satisfied our inclusion criteria were reviewed.Results. We reviewed 12 studies measuring both AFP and PIVKA-II responses in HCC patients treated in various ways. The results are presented by treatment modality.Conclusion. Measurement of AFP and PIVKA II marker levels before and after HCC treatment is clinically useful in monitoring of treatment outcomes and prognosis and in predicting recurrence and survival.

Impact of c-erbB-2 Protein on 5-year Survival Rate of Gastric Cancer Patients after Surgery: A Cohort Study and Meta-analysis

2015 ◽  
Vol 103 (3) ◽  
pp. 249-254 ◽  
Author(s):  
Hao Wu ◽  
Zhenzhai Cai ◽  
Guangrong Lu ◽  
Shuguang Cao ◽  
He Huang ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Lymph Node ◽  
Survival Rate ◽  
Lymph Node Metastasis ◽  
Protein Expression ◽  
Meta Analysis ◽  
Clinicopathological Characteristics ◽  
Node Metastasis ◽  
Positive Expression ◽  
Significant Difference

Objective To explore the association of c-erbB-2 protein expression with clinicopathological characteristics and prognosis of gastric cancer (GC) after surgery. Methods A total of 133 patients undergoing surgical resection for GC between March 2006 and January 2009 in the Second Affiliated Hospital of Wenzhou Medical University were included in this study. c-erbB-2 protein expression was determined by immunohistochemistry. Afterwards, a meta-analysis was performed to further confirm the association between c-erbB-2 protein expression and GC by employing stringent inclusion and exclusion criteria. All data analyses were conducted with STATA 12.0 and SPSS 19.0. Results There was no significant difference in c-erbB-2 expression among patients with various parameters including age, gender and histological types (all p>0.05). Among 133 GC patients, 32 patients presented c-erbB-2-positive expression and 101 presented c-erbB-2-negative expression (24.1% vs. 75.9%). The c-erbB-2-positive expression rate was significantly higher in GC tissues of patients with lymph node metastasis than those without. Similarly, a significant increase in c-erbB-2 expression was observed in well/moderately differentiated GC tissues compared with poorly differentiated GC. Patients with negative c-erbB-2 expression had a higher 5-year survival rate than those with positive c-erbB-2 expression, which was consistent with the results of the meta-analysis (OR = 0.54, 95% CI 0.37-0.80, p = 0.002). Conclusions Our study demonstrated that high expression of c-erbB-2 protein was strongly associated with lymph node metastasis, histological differentiation and 5-year survival rate in GC patients after surgery.

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