scholarly journals The Possible Role of TLR2 in Chronic Hepatitis B Patients with Precore Mutation

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Malihe Moradzadeh ◽  
Sirous Tayebi ◽  
Hossein Poustchi ◽  
Kourosh Sayehmiri ◽  
Parisa Shahnazari ◽  
...  
Keyword(s):  
Chronic Hepatitis ◽  
Hepatitis B ◽  
Chronic Hepatitis B ◽  
Direct Sequencing ◽  
Innate Immune ◽  
Enzyme Linked Immunosorbent Assay ◽  
B Virus ◽  
Toll Like Receptor 2 ◽  
Negative Chronic Hepatitis

Recognition mechanisms of innate immune response help to improve immunotherapeutic strategies in HBeAg-negative chronic hepatitis B (CHB). Toll-like receptor 2 (TLR2) is an important component of innate immunity. In this study, the frequency of precore mutations of the hepatitis B virus (HBV) and serum TLR2 were evaluated in CHB patients. Fifty-one patients with chronic hepatitis B, negative for HBeAg and detectable HBV DNA, were examined for the presence of mutations in pre-core region of HBV genome by direct sequencing. Serum TLR2 was measured by enzyme-linked immunosorbent assay. Interactions of truncated HBeAg and TLR2 proteins were evaluated with molecular docking software. The G1896A pre-core mutation were detected in 29 (57%) which was significantly associated with higher concentration of serum TLR2 in comparison with patients without this mutation (4.8±2.9 versus 3.4±2.2 ng/mL,P=0.03). There was also a significant correlation between serum ALT and TLR-2 (r=0.46;P=0.01). Docking results illustrated residues within the N-terminus of truncated HBeAg and TLR2, which might facilitate the interaction of these proteins. These findings showed the dominance of G1896A pre-core mutation of HBV variants in this community which was correlated with serum TLR2. Moreover TLR2 is critical for induction of inflammatory cytokines and therefore ALT elevation.

Detection of Hepatitis B Genotypes in HBsAg & HBeAg Carriers

2018 ◽  
Vol 7 (5) ◽  
Author(s):  
Salman Khan ◽  
Molly Madan
Keyword(s):  
Chronic Hepatitis ◽  
Viral Load ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Chronic Hepatitis B ◽  
Central Research ◽  
Elisa Kit ◽  
B Virus ◽  
Negative Chronic Hepatitis ◽  
Genotype A

Objective:- Hepatitis B is noteworthy medical issues that may include the late continuation of liver cirrhosis and hepatocellular carcinoma. The present study aimed for the detection and diffrentiation of Hepatitis B virus HBsAg inactive non-replicative carriers, HBeAg-positive inactive replicative carriers, active carriers & HBeAg-negative chronic hepatitis B by Real Time PCR and their genotyping Methods: This research conducted on 245 positive for HBsAg, 118 (48.16 %) were male and 127 (51.84%) were female patients, which was performed in central research station labortory of Microbiology at netaji subhash Chandra Bose subharti Medical College and Hospital, Meerut Between march 2016 to November 2017 The sera were separated and screened for HBsAg by ELISA kit. Positive samples for HBsAg were tested for HBeAg ELISA kit and DNA Viral load then sequenced for genotying Results:. Of the 245 HBsAg Positive case 55 (1.12%) were HBeAg positive. In 16 PCR positive and HBV genotyping, In HBsAg inactive Non-Replicative 37.5% (n=6) genotype-B and 6.25% (n=1) genotype-A, In HBeAg inactive Replicative 12.5% (n=2) genotype-B and 12.5% (n=2) genotype-A and In HBeAg Active Chronic Hepatitis B 18.75% (n=3) genotype-B and 12.5% (n=2) genotype-A were detected Conclusions: Management strategy, using HBsAg, HBeAg and HBV DNA viral load, seems adequate for the confirmation and diffrentiation of Hepatitis B virus inactive, active carriers & HBeAg-negative chronic hepatitis B patients and genotype B was more prevalent in comparission to genotype A. Distribution of carriers & genotypes, help physicians to prescribe proper antiviral/interferon therapy according to current genotyping pattern in this region Keywords: Hepatitis B virus, Carrier State, HBsAg, HBeAg, RT-PCR

Hepatitis B virus-specific T cell responses after stopping nucleos(t)ide analogue therapy in HBeAg-negative chronic hepatitis B

2018 ◽  
Vol 69 (3) ◽  
pp. 584-593 ◽  
Author(s):  
Franziska Rinker ◽  
Christine L. Zimmer ◽  
Christoph Höner zu Siederdissen ◽  
Michael P. Manns ◽  
Anke R.M. Kraft ◽  
...  
Keyword(s):  
Chronic Hepatitis ◽  
Hepatitis B Virus ◽  
T Cell ◽  
Hepatitis B ◽  
Chronic Hepatitis B ◽  
T Cell Responses ◽  
Cell Responses ◽  
B Virus ◽  
Negative Chronic Hepatitis

scholarly journals Hepatitis B virus surface antigen levels: A guide to sustained response to peginterferon alfa-2a in HBeAg-negative chronic hepatitis B

Hepatology ◽  
2008 ◽  
Vol 49 (4) ◽  
pp. 1141-1150 ◽  
Author(s):  
Maurizia Rossana Brunetto ◽  
Francesco Moriconi ◽  
Ferruccio Bonino ◽  
George K. K. Lau ◽  
Patrizia Farci ◽  
...  
Keyword(s):  
Chronic Hepatitis ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Chronic Hepatitis B ◽  
Surface Antigen ◽  
Sustained Response ◽  
Virus Surface ◽  
B Virus ◽  
Peginterferon Alfa ◽  
Negative Chronic Hepatitis

scholarly journals Role of Hepatitis B Virus Genotypes in Chronic Hepatitis B Exacerbation

2003 ◽  
Vol 37 (4) ◽  
pp. 593-597 ◽  
Author(s):  
Man-Fung Yuen ◽  
Erwin Sablon ◽  
Danny Ka-Ho Wong ◽  
He-Jun Yuan ◽  
Benjamin Chun-Yu Wong ◽  
...  
Keyword(s):  
Chronic Hepatitis ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Chronic Hepatitis B ◽  
B Virus ◽  
Virus Genotypes
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