scholarly journals Differential Influence of Components Resulting from Atmospheric-Pressure Plasma on Integrin Expression of Human HaCaT Keratinocytes

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Beate Haertel ◽  
Susanne Straßenburg ◽  
Katrin Oehmigen ◽  
Kristian Wende ◽  
Thomas von Woedtke ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Wound Healing ◽  
Atmospheric Pressure ◽  
Treatment Time ◽  
Atmospheric Pressure Plasma ◽  
Integrin Expression ◽  
Hacat Keratinocytes ◽  
Air Plasma ◽  
Pressure Plasma ◽  
Intracellular Ros

Adequate chronic wound healing is a major problem in medicine. A new solution might be non-thermal atmospheric-pressure plasma effectively inactivating microorganisms and influencing cells in wound healing. Plasma components as, for example, radicals can affect cells differently. HaCaT keratinocytes were treated with Dielectric Barrier Discharge plasma (DBD/air, DBD/argon), ozone or hydrogen peroxide to find the components responsible for changes in integrin expression, intracellular ROS formation or apoptosis induction. Dependent on plasma treatment time reduction of recovered cells was observed with no increase of apoptotic cells, but breakdown of mitochondrial membrane potential. DBD/air plasma increased integrins and intracellular ROS. DBD/argon caused minor changes. About 100 ppm ozone did not influence integrins. Hydrogen peroxide caused similar effects compared to DBD/air plasma. In conclusion, effects depended on working gas and exposure time to plasma. Short treatment cycles did neither change integrins nor induce apoptosis or ROS. Longer treatments changed integrins as important for influencing wound healing. Plasma effects on integrins are rather attributed to induction of other ROS than to generation of ozone. Changes of integrins by plasma may provide new solutions of improving wound healing, however, conditions are needed which allow initiating the relevant influence on integrins without being cytotoxic to cells.

Enhanced Long-Term Color Stability of Teeth Treated with Hydrogen Peroxide and Non-Thermal Atmospheric Pressure Plasma Jets

2014 ◽  
Vol 11 (11) ◽  
pp. 1010-1017 ◽  
Author(s):  
Seoul Hee Nam ◽  
Hyun Wook Lee ◽  
Jin Woo Hong ◽  
Hae June Lee ◽  
Gyoo Cheon Kim
Keyword(s):  
Hydrogen Peroxide ◽  
Atmospheric Pressure ◽  
Atmospheric Pressure Plasma ◽  
Color Stability ◽  
Plasma Jets ◽  
Pressure Plasma

scholarly journals Non-thermal atmospheric-pressure plasma can influence cell adhesion molecules on HaCaT-keratinocytes

2010 ◽  
Vol 20 (3) ◽  
pp. 282-284 ◽  
Author(s):  
Beate Haertel ◽  
Kristian Wende ◽  
Thomas von Woedtke ◽  
Klaus Dieter Weltmann ◽  
Ulrike Lindequist
Keyword(s):  
Cell Adhesion ◽  
Adhesion Molecules ◽  
Atmospheric Pressure ◽  
Cell Adhesion Molecules ◽  
Atmospheric Pressure Plasma ◽  
Hacat Keratinocytes ◽  
Pressure Plasma

scholarly journals Analysis of Plasma-activated Medium (PAM) in aqueous solution by an Atmospheric Pressure Plasma Jet (APPJ)

2018 ◽  
Vol 197 ◽  
pp. 02013 ◽  
Author(s):  
Andi Wibowo Kinandana ◽  
Sumariyah Sumariyah ◽  
Muhammad Nur
Keyword(s):  
Hydrogen Peroxide ◽  
Species Composition ◽  
Exposure Time ◽  
Atmospheric Pressure ◽  
Atmospheric Pressure Plasma ◽  
Plasma Jet ◽  
Reactive Species ◽  
Atmospheric Pressure Plasma Jet ◽  
Liquid Media ◽  
Pressure Plasma

Plasma-activated medium (PAM) has been produced by exposing a liquid media to Argon plasma jet. The jet plasma exposure to liquid media has produced reactive Oxygen species (ROS) in liquid phase. This study aims to determine the number of reactive species in plasma-activated medium. An atmospheric pressure plasma jet (APPJ) was generated with a dielectric barrier discharge (DBD) column by AC high voltage. Some parameters varied including exposure time; i.e. 5, 10, 15, 20, 25, and 30 min; and the distance between reactor and active media; i.e. 1, 2 and 3 cm. Some analysis conducted including variation of exposure times, the distances of reactor to PAM which affect produced concentration, and the reactive species composition in plasma-activated medium. In addition, temperature characteristics, pH levels, dissolved ozone and dissolved hydrogen peroxide concentrations were also observed in this study. The results showed that increased exposure time resulted in decreased pH, increased temperature and increased concentrations of ozone and hydrogen peroxide. The maximum reactive species composition was obtained at the distance between reactor and plasma-activated medium of 2 cm. Maximum reactive species composition obtained in this study has temperature of 29-30 Celsius degrees; pH 3.5; dissolved ozone 2.97 ppm; and Hydrogen Peroxide 215 ppm.

scholarly journals Cold Atmospheric Pressure Plasma in Wound Healing and Cancer Treatment

2020 ◽  
Vol 10 (19) ◽  
pp. 6898
Author(s):  
Lars Boeckmann ◽  
Mirijam Schäfer ◽  
Thoralf Bernhardt ◽  
Marie Luise Semmler ◽  
Ole Jung ◽  
...  
Keyword(s):  
Wound Healing ◽  
Cancer Therapy ◽  
Cancer Treatment ◽  
Atmospheric Pressure ◽  
Atmospheric Pressure Plasma ◽  
Reactive Species ◽  
Therapeutic Effects ◽  
Preclinical Research ◽  
Pressure Plasma ◽  
Cold Atmospheric Pressure Plasma

Plasma medicine is gaining increasing attention and is moving from basic research into clinical practice. While areas of application are diverse, much research has been conducted assessing the use of cold atmospheric pressure plasma (CAP) in wound healing and cancer treatment—two applications with entirely different goals. In wound healing, a tissue-stimulating effect is intended, whereas cancer therapy aims at killing malignant cells. In this review, we provide an overview of the latest clinical and some preclinical research on the efficacy of CAP in wound healing and cancer therapy. Furthermore, we discuss the current understanding of molecular signaling mechanisms triggered by CAP that grant CAP its antiseptic and tissue regenerating or anti-proliferative and cell death-inducing properties. For the efficacy of CAP in wound healing, already substantial evidence from clinical studies is available, while evidence for therapeutic effects of CAP in oncology is mainly from in vitro and in vivo animal studies. Efforts to elucidate the mode of action of CAP suggest that different components, such as ultraviolet (UV) radiation, electromagnetic fields, and reactive species, may act synergistically, with reactive species being regarded as the major effector by modulating complex and concentration-dependent redox signaling pathways.

scholarly journals Adhesion between Epoxy Resin-Based Fiber Post and Dental Core Resin Improved by Non-Thermal Atmospheric Pressure Plasma

2020 ◽  
Vol 10 (7) ◽  
pp. 2535
Author(s):  
Hyoung-Sik Kim ◽  
Song-Yi Yang ◽  
Eun Ha Choi ◽  
Kwang-Mahn Kim ◽  
Jae-Sung Kwon
Keyword(s):  
Contact Angle ◽  
Bond Strength ◽  
Epoxy Resin ◽  
Atmospheric Pressure ◽  
Shear Bond Strength ◽  
Treatment Time ◽  
Atmospheric Pressure Plasma ◽  
Fiber Post ◽  
Pressure Plasma ◽  
Push Out

The purpose of the study was to evaluate the adhesion between dental core resin and epoxy resin-based fiber post after treatment with non-thermal atmospheric pressure plasma (NTAPP) and compare with conventional methods of epoxy resin-based fiber post treatments. Contact angle was measured on the surface of epoxy resin before and after NTAPP treatment and X-ray photoelectron spectroscopy was used to analyze the surface chemistry. Finally, two shear bond strength tests were carried out; shear bond strength between core resin and epoxy resin for comparison between NTAPP treated and untreated sample, and push-out shear bond strength between core resin and NTAPP treated commercially available epoxy resin-based fiber post for comparison between NTAPP treated samples with conventionally treated samples. Contact angle on the surface of epoxy resin generally decreased with increasing NTAPP treatment time with presence of surface chemical changes. Also, there was significantly higher shear bond strength and push-out shear bond strength between epoxy resin and core resin for NTAPP treated epoxy resin, even to the conventionally treated epoxy resin-based fiber post with hydrofluoric acid or silane. In conclusion, new technology of NTAPP has potential for application on the epoxy resin-based fiber post to improve endodontic restoration success rate.

Modeling the Inactivation of Bacillus cereus in Tiger Nut Milk Treated with Cold Atmospheric Pressure Plasma

2019 ◽  
Vol 82 (11) ◽  
pp. 1828-1836 ◽  
Author(s):  
ALIYU IDRIS MUHAMMAD ◽  
RUILING LV ◽  
XINYU LIAO ◽  
WEIJUN CHEN ◽  
DONGHONG LIU ◽  
...  
Keyword(s):  
Bacillus Cereus ◽  
Atmospheric Pressure ◽  
Treatment Time ◽  
Atmospheric Pressure Plasma ◽  
Titratable Acidity ◽  
Weibull Model ◽  
Input Power ◽  
Inactivation Kinetics ◽  
Pressure Plasma ◽  
Cold Atmospheric Pressure Plasma

ABSTRACT The impact of cold atmospheric pressure plasma treatment on the inactivation kinetics of Bacillus cereus ATCC 14579 and the resulting quality changes was investigated in tiger nut (Cyperus esculentus L.) milk (TNM). The effect of input power (39, 43, and 46 W) and treatment time (0 to 270 s) was fitted using the Weibull model to represent the microbial kinetic inactivation in the treated TNM. Inactivation efficacy increased with an increase in treatment time and input power. A 5.28-log reduction was achieved at 39 to 46 W without significant changes in titratable acidity, whereas no reduction in titratable acidity was observed in the pasteurized sample. The inactivation kinetics was adequately described by the Weibull model. Higher input power of 43 and 46 W and 120 s of treatment resulted in marked decreases in pH, flavonoid concentration, and antioxidant activity compared with those parameters in pasteurized TNM. Increases in total color difference and phenolic concentrations also were observed. The results indicate that these changes were caused by the immanent plasma reactive species. This study provides valuable inactivation kinetics information for food safety assessment studies of B. cereus vegetative cells in TNM.

Hydrogen peroxide in lactate solutions irradiated by non-equilibrium atmospheric pressure plasma

2020 ◽  
Author(s):  
Yang Liu ◽  
Kenji Ishikawa ◽  
Camelia Miron ◽  
Hiroshi Hashizume ◽  
Hiromasa Tanaka ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Atmospheric Pressure ◽  
Atmospheric Pressure Plasma ◽  
Pressure Plasma ◽  
Non Equilibrium
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