scholarly journals Circulating miR-583 and miR-663 Refer to ZHENG Differentiation in Chronic Hepatitis B

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Hui Zhang ◽  
Yan Guan ◽  
Yi-Yu Lu ◽  
Yi-Yang Hu ◽  
Shuang Huang ◽  
...  
Keyword(s):  
Chronic Hepatitis ◽  
Hepatitis B ◽  
Chronic Hepatitis B ◽  
Target Genes ◽  
Enrichment Analysis ◽  
Deficiency Syndrome ◽  
Mapk Signaling ◽  
Pathway Enrichment Analysis ◽  
Roc Curve Analysis ◽  
Zheng Differentiation

Traditional Chinese medicine (TCM) ZHENG as the key pathological principle is to understand the human homeostasis and guide TCM treatment. Here, circulating microRNAs (miRNAs) were utilized to differentiate between ZHENGs including liver-gallbladder dampness-heat syndrome (LGDHS) and liver-kidney yin deficiency syndrome (LKYDS) in chronic hepatitis B (CHB). Sera samples of CHB patients with LGDHS (n=35), LKYDS (n=24), and healthy controls (Ctrls,n=21) were analyzed by microarray and real-time RT-PCR. Receiver-operator characteristic (ROC) curves were established to evaluate the levels of serum miRNA for discriminating LGDHS and LKYDS. The target genes of miRNAs were predicted by TargetScan. Gene Ontology (GO) and pathways were analyzed using DAVID tool. The results showed that 22 miRNAs were differentially expressed between LGDHS and LKYDS (fold change>2.0 andP<0.01). Circulating miR-583 and miR-663 were significantly higher (P<0.001) in CHB patients with LGDHS than those with LKYDS and Ctrls. ROC curve analysis revealed that miR-583 and miR-663 were sensitive and specific enough to distinguish LGDHS from LKYDS. Pathway enrichment analysis indicated that 354 putative targets for miR-583 and 68 putative targets for miR-663 were mainly involved in Axon guidance, Neurotrophin, and MAPK signaling pathway. miR-583 and miR-663 may be potential markers for ZHENG differentiation in CHB.

scholarly journals Expression Profiling of Cellular MicroRNA in Asymptomatic HBsAg Carriers and Chronic Hepatitis B Patients

2017 ◽  
Vol 2017 ◽  
pp. 1-17 ◽  
Author(s):  
Xianliang Hou ◽  
Yan Liang ◽  
Jianing Chen ◽  
Yingfeng Wei ◽  
Ping Zeng ◽  
...  
Keyword(s):  
Chronic Hepatitis ◽  
Hepatitis B ◽  
Signaling Pathway ◽  
Chronic Hepatitis B ◽  
Target Genes ◽  
Mononuclear Cells ◽  
Expression Profiles ◽  
Differentially Expressed ◽  
Pathway Enrichment Analysis ◽  
Peripheral Blood Mononuclear

Background. MicroRNAs (miRNAs) may serve as potential molecular markers to predict liver injury resulting from chronic hepatitis B (CHB). In the present study, we want to study the expression profile and clinical significance of miRNAs at different stages of CHB virus infection. Methods. Using miRNA microarray, we investigated the global expression profiles of cellular miRNA in asymptomatic hepatitis B antigen carriers (ASCs) and CHB patients, compared with healthy controls (HCs). Results. We identified 79 and 203 differentially expressed miRNAs in the peripheral blood mononuclear cells of ASCs and CHB patients compared to HCs, respectively. Some of these miRNAs were common to ASCs and CHB patients, but another set of miRNAs that showed differential expression between ASCs and CHB patients was also identified. Gene ontology and pathway enrichment analysis showed that the target genes of the identified miRNAs played a role in important biological functions, such as learning or memory, cell-cell adherens junction, ion channel inhibitor activity, TGF-beta signaling pathway, and p53 signaling pathway. Conclusion. We identified some significant differentially expressed miRNA in different phases of HBV infection, which might serve as biomarkers or therapeutic targets in the future.

Mothers and Hepatitis B

PEDIATRICS ◽  
1983 ◽  
Vol 72 (2) ◽  
pp. 265-265
Author(s):  
Philip Rosenthal
Keyword(s):  
Chronic Hepatitis ◽  
Hepatitis B ◽  
Chronic Hepatitis B ◽  
Immune Deficiency Syndrome ◽  
Deficiency Syndrome ◽  
Hepatitis B Vaccine ◽  
Hepatitis B Virus Infection ◽  
Chronic Hepatitis B Virus ◽  
B Virus ◽  
Acquired Immune Deficiency

I read with interest the commentary on prevention of chronic hepatitis B virus infection.1 An important issue that was not discussed concerns the use of the hepatitis B vaccine and transmission of the newly recognized acquired immune deficiency syndrome (AIDS). The etiology of AIDS is unknown. AIDS occurs among the populations that donate plasma for hepatitis B vaccine manufacture.2,3 The safety of plasma donated for hepatitis B vaccine by persons with chronic hepatitis B who may have unrecognized or early AIDS is not known.

scholarly journals Serum cytokine profiling analysis for zheng differentiation in chronic hepatitis B

2015 ◽  
Vol 10 (1) ◽  
Author(s):  
Yi-Yu Lu ◽  
Yu Zhao ◽  
Ya-Nan Song ◽  
Shu Dong ◽  
Bin Wei ◽  
...  
Keyword(s):  
Chronic Hepatitis ◽  
Hepatitis B ◽  
Chronic Hepatitis B ◽  
Serum Cytokine ◽  
Zheng Differentiation ◽  
Profiling Analysis ◽  
Cytokine Profiling

scholarly journals Diagnostic Efficacy of FibroScan for Liver Inflammation in Patients with Chronic Hepatitis B --- A Single-Center Study with 1,185 Liver Biopsies as Controls

2021 ◽  
Author(s):  
Kaiping Jiang ◽  
Lei Zhang ◽  
Jianhong Li ◽  
Hongtao Hu ◽  
Qinghua Huang ◽  
...  
Keyword(s):  
Chronic Hepatitis ◽  
Hepatitis B ◽  
Liver Biopsy ◽  
Chronic Hepatitis B ◽  
Liver Stiffness ◽  
Roc Curves ◽  
Diagnostic Value ◽  
Liver Inflammation ◽  
Roc Curve Analysis ◽  
Diagnostic Efficacy

Abstract Background: Noninvasive diagnostic technologies that can dynamically monitor changes in liver inflammation are highly important for the management of chronic hepatitis B (CHB) patients and thus warrant further exploration. This study assessed the diagnostic efficacy of FibroScan for liver inflammation in CHB patients.Methods: A total of 1185 patients were selected, and ultrasound-guided liver biopsy was performed within one month after the FibroScan test. The liver stiffness measurement (LSM), the reliability criteria (IQR/M) of LSM, the quality of liver biopsy (complete portal area, PA), and the liver inflammation grades were the main observation items of this study. With liver biopsy as the control, the diagnostic efficacy of FibroScan for liver inflammation in CHB patients was evaluated by receiver operating characteristic (ROC) curve analysis.Results: Significant differences in the LSM of FibroScan were observed among different grades of liver inflammation (P<0.0001). Liver biopsy (PA>10) served as the control, and the cutoff point and the area under ROC curves (AUCs) of the LSMs for different inflammation grades were as follows: G2, 8.6 kPa, 0.775; G3 9.8 kPa, 0.818; and G4, 11.0 kPa; 0.832. With LSM cutoff values of 8.6 kPa, 9.8 kPa and 11.0 kPa, FibroScan showed certain diagnostic value for CHB patients with G2, G3 and G4 liver inflammation, especially those with G4 inflammation. Conclusions: The results of this study preliminarily showed that, in addition to liver fibrosis, FibroScan could evaluate liver inflammation in CHB patients in a noninvasive manner.

scholarly journals The molecular mechanism of TiaoGanYiPi formula for treatment of chronic hepatitis B by network pharmacology and molecular docking verification

2020 ◽  
Author(s):  
Xu Cao ◽  
Xiaobin Zao ◽  
Baiquan Xue ◽  
Hening Chen ◽  
Jiaxin Zhang ◽  
...  
Keyword(s):  
Chronic Hepatitis ◽  
Molecular Docking ◽  
Hepatitis B ◽  
Chronic Hepatitis B ◽  
Target Genes ◽  
Regulation Of Gene Expression ◽  
Interaction Network ◽  
Network Pharmacology ◽  
Biological Processes ◽  
The Relationship

Abstract The Chinese herbal formula Tiao-Gan-Yi-Pi (TGYP) showed effective against Chronic Hepatitis B (CHB). In this study, we aimed to clarify the mechanisms and potential targets between TGYP and CHB through network pharmacology and molecular docking verification. The compounds of TGYP were identified in the TCMSP and CNKI databases, and their putative targets were predicted through SwissTargetPrediction and STITCH databases. The targets of CHB were obtained from the GeneCards, NCBI Gene, and DisGeNET databases. The above mentioned data were visualized using Cytoscape, and molecular docking showed the relationship between them. The expression of key targets was verified in GEO databases. Hence, we screened out 11 TGYP-related key targets for CHB included ABL1, CASP8, CCNA2, CCNB1, CDK4, CDKN1A, EP300, HIF1A, IGF1R, MAP2K1 and PGR. The key targets were predominantly enriched in the cancer, cell cycle and hepatitis B pathways and involved in the positive regulation of fibroblast proliferation, signal transduction, and negative regulation of gene expression biological processes, and expression of key target genes was related to HBV infection and liver inflammation. Through this newly constructed interaction network between TGYP and CHB, we identified active compounds and targets which could be further used for providing clinical guidance.

scholarly journals Comparative Analysis of Clinical and Medication Information between Chronic Hepatitis B Patients with Damp Heat Syndrome and Spleen Deficiency Syndrome

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Qiao-Hong Liu ◽  
Bin-Bin Zhang ◽  
Lin Xu ◽  
Xiao-Ping Shen ◽  
Ya-Mei Hai ◽  
...  
Keyword(s):  
Chronic Hepatitis ◽  
Hepatitis B ◽  
Chronic Hepatitis B ◽  
Deficiency Syndrome ◽  
Individual Level ◽  
Spleen Deficiency ◽  
Medication Information ◽  
Level Data ◽  
Tcm Syndrome ◽  
Heat Syndrome

Traditional Chinese medicine (TCM) has a long history in the treatment of chronic hepatitis B (CHB) based on the syndrome identification. Previous studies reported CHB patients with damp-heat (DH) syndrome accompanied with a severe liver function damage, but lacked the medication analysis. In this study, we analyzed 999 CHB patients with unidentified individual-level data from database to explore clinical features of two common syndromes of CHB patients based on the real world. Compared with the spleen deficiency (SD) syndrome, the CHB patients with DH syndrome had a significantly higher level of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) ( P < 0.05 ) but took more immunomodulators and hepatoprotective drugs ( P < 0.1 ). Similarly, in the follow-up of 207 patients after 3 months, the improvement trend of ALT and AST of patients with sustained SD syndrome was significantly better than those whose TCM syndrome changed from SD to DH ( P < 0.05 ). The logistic model indicated DH syndrome was a significant negative factor for reducing ALT level in CHB patients (OR = 4.854, P = 0.032 ). This study suggests that CHB patients with DH syndrome have potentially more serious and sustained liver damage than the SD syndrome, which provides a reference for the personalized management of CHB patients from the perspective of TCM syndromes.

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