scholarly journals Copper Enhanced Monooxygenase Activity and FT-IR Spectroscopic Characterisation of Biotransformation Products in Trichloroethylene Degrading Bacterium:Stenotrophomonas maltophiliaPM102

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Piyali Mukherjee ◽  
Pranab Roy
Keyword(s):  
Maximum Activity ◽  
Crude Enzyme ◽  
Monooxygenase Activity ◽  
Ammonium Sulphate Precipitation ◽  
Oxygenase Activity ◽  
Biotransformation Products ◽  
Ft Ir ◽  
Tce Degradation ◽  
Ir Spectroscopic ◽  
Ft Ir Spectroscopy

Stenotrophomonas maltophiliaPM102 (NCBI GenBank Acc. no. JQ797560) is capable of growth on trichloroethylene as the sole carbon source. In this paper, we report the purification and characterisation of oxygenase present in the PM102 isolate. Enzyme activity was found to be induced 10.3-fold in presence of 0.7 mM copper with a further increment to 14.96-fold in presence of 0.05 mM NADH. Optimum temperature for oxygenase activity was recorded at36∘C. The reported enzyme was found to have enhanced activity at pH 5 and pH 8, indicating presence of two isoforms. Maximum activity was seen on incubation with benzene compared to other substrates like TCE, chloroform, toluene, hexane, and petroleum benzene.KmandVmaxfor benzene were 3.8 mM and 340 U/mg/min and those for TCE were 2.1 mM and 170 U/mg/min. The crude enzyme was partially purified by ammonium sulphate precipitation followed by dialysis. Zymogram analysis revealed two isoforms in the 70% purified enzyme fraction. The activity stain was more prominent when the native gel was incubated in benzene as substrate in comparison to TCE. Crude enzyme and purified enzyme fractions were assayed for TCE degradation by the Fujiwara test. TCE biotransformation products were analysed by FT-IR spectroscopy.

scholarly journals Researching carbon varieties from season-caused shell for use of H2O2 properties to treat lead

2020 ◽  
Vol 4 (2) ◽  
pp. first
Author(s):  
Dao Minh Trung ◽  
Tram Thi Thanh Nguyen
Keyword(s):  
Heavy Metals ◽  
Functional Groups ◽  
Research Results ◽  
Chemical Mechanisms ◽  
Sem Image ◽  
Study Results ◽  
Ft Ir ◽  
Ir Spectroscopic ◽  
Physical And Chemical ◽  
Ft Ir Spectroscopy

The study assumes lead wastewater treatment with initial concentration fixed at 30 ppm and research material, denatured coal prepared from macadamia seed shell activated by heating, and finally variable Coal calculation with H2O2 agent. The coal was modified by soaking coal in 25% H2O2 solution for 48 hours. Coal after denatured for adsorption results reached 266.26 mg/g, the research results show similarities with some previous studies. On the other hand, when analyzing the infrared spectrum of coal, the FT-IR spectroscopic results showed that after denatured coal, there were some functional groups such as OH-, C-O, C=O, C-N, functional groups capable of participating in the adsorption of heavy metals in wastewater. When observing the SEM image, the photo results of the material show that the surface of the material is rough, with many pores and different sizes evenly distributed on the surface. From SEM image results and FT-IR spectroscopy results show that the material has the ability to adsorb heavy metals by many physical and chemical mechanisms. Indeed, when researching the application of materials to the treatment of lead heavy metals in presumptive wastewater, the research results show that lead treatment efficiency reaches 94.05% when surveyed at the same optimal conditions at pH = 6 with dose of 0.4 g/L and duration of 60 minutes. The control shows that the study results are similar to some previous research results, from which it can be confirmed that the modified coal material is capable of Pb (II) treatment in wastewater.

Crosslinking of Gelatin in the Reaction with Formaldehyde: An FT-IR Spectroscopic Study

1996 ◽  
Vol 50 (10) ◽  
pp. 1314-1318 ◽  
Author(s):  
T. Salsa ◽  
M. E. Pina ◽  
J. J. C. Teixeira-Dias
Keyword(s):  
Principal Component Regression ◽  
Principal Component ◽  
Potassium Bromide ◽  
Crosslinking Reaction ◽  
Spectral Changes ◽  
Principal Component Regression Analysis ◽  
Ft Ir ◽  
Ir Spectroscopic ◽  
Kinetics Of ◽  
Ft Ir Spectroscopy

The reaction of an aqueous solution of formaldehyde with gelatin dispersed in a potassium bromide pellet is monitored in real time by FT-IR spectroscopy. Principal component regression analysis of the spectra recorded at different times is carried out. On the whole, the latter results and the observed spectral changes are in agreement with a previously reported interpretation for the kinetics of the crosslinking reaction of gelatin with formaldehyde, according to which the reaction is initialized by the lysine–methylol formation and is subsequently followed by arginine–methylol, which, in turn, reacts with lysine–methylol to originate arginine–lysine crosslinks.

scholarly journals Fourier transform infrared (FT-IR) spectroscopic investigations of four agarophytes from northern Arabian sea

2019 ◽  
Vol 48 (4) ◽  
pp. 925-932
Author(s):  
Laila Shahnaz ◽  
Hina Shehnaz ◽  
Amir Haider
Keyword(s):  
Fourier Transform ◽  
Arabian Sea ◽  
Gel Strength ◽  
Hypnea Musciformis ◽  
High Quality ◽  
Northern Arabian Sea ◽  
Ft Ir ◽  
Ir Spectroscopic ◽  
First Time ◽  
Ft Ir Spectroscopy

The fresh specimens of Champia compressa Harvey, Gelidium usmanghanii Afaq-Husain and M. Shameel, Gracilaria foliifera (Forsskål) Børgesen and Hypnea musciformis (Wulfen) J. V. Lamouroux, were collected from the coastal areas of Karachi (Pakistan) and their yield of agar was observed. With preextraction technique the percentage of algal yield was found to range from 13 to 28%. High quality gel strength was determined by modulus of elasticity i.e. (550 – 612g/cm2) and 1090 kPa and extraction time was 2 hrs. The structure of agar and agar contents were investigated by FT-IR spectroscopy for the first time from Pakistan. The results revealed some interesting characters (non sulphated β-D galacto pyranose residues, 3,6 anhydro galactose vibration), which were not reported earlier.

FT-IR spectroscopic and thermal study of waterborne polyurethane-acrylate leather coatings using tartaric acid as an ionomer

e-Polymers ◽  
2016 ◽  
Vol 16 (6) ◽  
pp. 463-474 ◽  
Author(s):  
Ali Bahadur ◽  
Muhammad Shoaib ◽  
Aamer Saeed ◽  
Shahid Iqbal
Keyword(s):  
Tartaric Acid ◽  
Waterborne Polyurethane ◽  
Hexamethylene Diisocyanate ◽  
Polyurethane Acrylate ◽  
Chain Extenders ◽  
Ft Ir ◽  
Waterborne Polyurethane Acrylate ◽  
End Capping ◽  
Ir Spectroscopic ◽  
Ft Ir Spectroscopy

AbstractWaterborne polyurethane (WDPU) comprising polycaprolactone diol and hexamethylene diisocyanate was prepared by using tartaric acid (TA) as an ionomer. WDPU was further modified with polymethyl methacrylate (PMMA). Hybrid WDPU-PMMA coatings were formed by using unsaturate end capping agents such as 2-hydroxyethylmethacrylate. WDPU-PMMA blend coatings were formed by using chain extenders such as ethylene glycol, ethylene diamine and mixed with PMMA. Thermal behavior and structural characterizations were investigated by means of thermogravimetric analysis and Fourier transformm infrared (FT-IR) spectroscopy, respectively. Chemical and solvent resistance was checked against various chemicals and solvents. Bio-stability was evaluated in CoCl2/H2O2 solution. Gloss, film flexibility, tackiness, abrasion resistance and adhesive prosperities of WDPU-PMMA coatings were also checked by applying these coatings on a leather sheet.

scholarly journals FT-IR Spectroscopic Characteristics of Ganoderma lucidum Secondary Metabolites

2020 ◽  
pp. 1-8
Author(s):  
B. Sangeetha ◽  
A. S. Krishnamoorthy ◽  
D. Amirtham ◽  
D. Jeya Sundara Sharmila ◽  
P. Renukadevi ◽  
...  
Keyword(s):  
Ir Spectroscopy ◽  
Ganoderma Lucidum ◽  
Antibacterial Agent ◽  
Chemical Nature ◽  
Ganoderic Acid ◽  
Spectroscopic Characteristics ◽  
Ft Ir ◽  
Ir Spectroscopic ◽  
Stretching Intensity ◽  
Ft Ir Spectroscopy

Ganoderma lucidum is an important medicinal mushroom widely used in pharmaceuticals for their antiviral, antibacterial, antifungal, anticancer and immunoregulatory properties and also used in agriculture as an antiviral and antibacterial agent. Fourier Transform Infrared (FT-IR) spectroscopy is a tool widely used in the researches for the identification of organic compounds in the organism. In our study, we used FT-IR Spectroscopy for determination the chemical nature of Ganoderma lucidum, and their standards Squalene and Ganoderic acid A. In the FT-IR spectrum for the G. lucidum obtained in the region of 3782.69 cm-1 to 568.898 cm-1 the absorptions peak represents the alcohols, halogens, silicon and phosphorus groups present in the sample. In the squalene standard, ketones, amides, and guanidine, nitrile and azine stretches were found in the wavelength 1655-1550 cm-1. In the Ganoderic acid A sulfur compounds with weak stretching intensity were obtained in the wavelength 500-400 cm-1. We have concluded that FT-IR spectroscopy is an effective method to analyze the chemical nature of the organic groups present in the samples.

Spectral Investigation on the Growth and Characterization of NLO Crystals of Thiourea Succinic Acid Single Crystal.

2015 ◽  
Vol 2 (2) ◽  
pp. 70-73
Author(s):  
Kannan.P ◽  
Thambidurai.S ◽  
Suresh.N
Keyword(s):  
Single Crystal ◽  
Succinic Acid ◽  
Slow Evaporation Technique ◽  
Absorption Studies ◽  
Dta Analysis ◽  
Evaporation Technique ◽  
Ft Ir ◽  
Ir Spectroscopic ◽  
Thermal Stability Of

Growth of optically transparent single crystals of thiourea succinic acid (TUSA) was grown successfully from aqueous solution by slow evaporation technique. The crystal structure was elucidated using the single crystal XRD. The various functional groups and the modes of vibrations were identified by FT-IR spectroscopic analysis. The optical absorption studies indicate that the optical transparency window is quite wide making its suitable for NLO applications. Thermal stability of the crown crystal carried out by TGA-DTA analysis.

scholarly journals The Preparation, Thermal Properties, and Fire Property of a Phosphorus-Containing Flame-Retardant Styrene Copolymer

Materials ◽  
2019 ◽  
Vol 13 (1) ◽  
pp. 127 ◽  
Author(s):  
Yu Sun ◽  
Yazhen Wang ◽  
Li Liu ◽  
Tianyuan Xiao
Keyword(s):  
Thermal Properties ◽  
Thermogravimetric Analysis ◽  
Flame Retardant ◽  
Oxygen Index ◽  
Limiting Oxygen Index ◽  
Chemical Structures ◽  
1H Nuclear Magnetic Resonance ◽  
Phosphorus Containing ◽  
Ft Ir ◽  
Ft Ir Spectroscopy

A 9,10-dihydro-9-oxa-10-phosphaphenanthrene 10-oxide (DOPO) acrylate, (6-oxidodibenzo [c,e][1,2] oxaphosphinin-6-yl) methyl acrylate (DOPOAA), has been prepared. Copolymers of styrene (St) and DOPOAA were prepared by emulsion polymerization. The chemical structures of copolymers containing levels of DOPOAA were verified using Fourier transform infrared (FT-IR) spectroscopy and 1H nuclear magnetic resonance (1H-NMR) spectroscopy. The thermal properties and flame-retardant behaviors of DOPO-containing monomers and copolymers were observed using thermogravimetric analysis and micro calorimetry tests. From thermogravimetric analysis (TGA), it was found out that the T5% for decomposition of the copolymer was lower than that of polystyrene (PS), but the residue at 700 °C was higher than that of PS. The results from micro calorimetry (MCC) tests indicated that the rate for the heat release of the copolymer combustion was lower than that for PS. The limiting oxygen index (LOI) for combustion of the copolymer rose with increasing levels of DOPOAA. These data indicate that copolymerization of the phosphorus-containing flame-retardant monomer, DOPOAA, into a PS segment can effectively improve the thermal stability and flame retardancy of the copolymer.

Multiscale exploration of hydrocarbons adsorption and hopping through the ZSM-5 channels - from Monte Carlo modelling to experiment

2021 ◽  
Author(s):  
Maciej Strzempek ◽  
Karolina A. Tarach ◽  
Kinga Góra-Marek ◽  
Fernando Rey ◽  
Miguel Palomino ◽  
...  
Keyword(s):  
Monte Carlo ◽  
Ir Spectroscopy ◽  
Aliphatic Hydrocarbons ◽  
Adsorption Studies ◽  
Ft Ir ◽  
Gravimetric Adsorption ◽  
Hydrocarbons Adsorption ◽  
Ft Ir Spectroscopy

Abstract In this article the results of the statistical MC modelling corroborated by the FT-IR spectroscopy and gravimetric adsorption studies of the low aliphatic hydrocarbons in ZSM-5 (Si/Al =28 or...

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