scholarly journals Simultaneous Determination of Prasugrel and Aspirin by Second Order and Ratio First Order Derivative Ultraviolet Spectrophotometry

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Shahabuddin N. Alvi ◽  
Mehul N. Patel ◽  
Prakash B. Kathiriya ◽  
Bhavna A. Patel ◽  
Shraddha J. Parmar
Keyword(s):  
Simultaneous Determination ◽  
Second Order ◽  
Order Derivative ◽  
Zero Crossing ◽  
First Order ◽  
Spectrophotometric Methods ◽  
Relative Standard ◽  
Derivative Method ◽  
Derivatives Of

Two simple, accurate, and precise UV derivative spectrophotometric methods for the simultaneous determination of Prasugrel and Aspirin in synthetic mixture form have been developed. The first method involves measurement of second order derivative spectra of Prasugrel and Aspirin. The zero crossing wavelengths 267.62 nm and 252.40 nm were selected for estimation of Prasugrel and Aspirin, respectively. In the second method, the first order derivatives of ratio spectra were calculated and used for the determination of Prasugrel and Aspirin by measuring the peak intensity at 268 nm and 290 nm, respectively. The methods were validated as per the ICH guideline Q2 (R1). Beer’s law is followed in the range of 5–45 μg/mL for Prasugrel and 25–150 μg/mL for Aspirin by second order derivative method and 6–22 μg/mL for Prasugrel and 45–165 μg/mL for Aspirin by ratio first order derivative method. The recovery studies confirmed the accuracy of the methods. Relative standard deviations for repeatability and inter- and intraday assays were less than 2%. Hence, the described derivative spectrophotometric methods are simple, accurate, precise, and excellent alternatives to sophisticated chromatographic techniques and can be potentially used for the simultaneous determination of Prasugrel and Aspirin in combined dosage form.

scholarly journals Derivative spectrophotometric determination of acetamiprid in the presence of 6-chloronicotinic acid

2012 ◽  
Vol 77 (7) ◽  
pp. 911-917 ◽  
Author(s):  
Valéria Guzsvány ◽  
Sanja Lazic ◽  
Natasa Vidakovic ◽  
Zsigmond Papp
Keyword(s):  
Simultaneous Determination ◽  
Spectrophotometric Determination ◽  
Spectrophotometric Method ◽  
Detection Limits ◽  
Model Systems ◽  
Order Derivative ◽  
Zero Crossing ◽  
First Order ◽  
Relative Standard

A simple first-order derivative spectrophotometric method was developed for the simultaneous determination of acetamiprid and 6-chloronicotinic acid (6-CNA) at pH 7.0. By using the zero-crossing approach, acetamiprid was determined at 269.0 nm and 6-CNA at 216.0 nm with the detection limits of 7.19x10-7 and 8.25x10-7 mol dm-3, respectively and relative standard deviations not exceeding 1.2% in the case of model systems.

scholarly journals Three Different Spectrophotometric Methods for Simultaneous Determination of Pyriproxyfen and Chlorothalonil Residues in Cucumber and Cabbage Samples

2019 ◽  
Vol 2019 ◽  
pp. 1-11
Author(s):  
Shilan A. Omer ◽  
Nabil A. Fakhre
Keyword(s):  
Simultaneous Determination ◽  
Simultaneous Estimation ◽  
Zero Crossing ◽  
Spectrophotometric Methods ◽  
First Derivative ◽  
The Third ◽  
Mean Centering ◽  
Relative Standard ◽  
One Way Anova

In this study, three simple and accurate spectrophotometric methods for simultaneous determination of pyriproxyfen and chlorothalonil residues in cucumbers and cabbages grown in experimental greenhouse were studied. The first method was based on the zero-crossing technique measurement for first and second derivative spectrophotometry. The second method was based on the first derivative of the ratio spectra. However, the third method was based on mean centering of ratio spectra. These procedures lack any previous separation steps. The calibration curves for three spectrophotometric methods are linear in the concentration range of 1–30 μg·mL−1 and 0.5–7 μg·mL−1 for pyriproxyfen and chlorothalonil successively. The recoveries ranged from 82.12–97.40% for pyriproxyfen and 81.51–97.04% for chlorothalonil with relative standard deviations less than 4.95% and 5.45% in all instances for pyriproxyfen and chlorothalonil, respectively. The results obtained from the proposed methods were compared statistically by using one-way ANOVA, and the results revealed there were no significant differences between ratio spectra and mean centering methods with the zero-crossing technique. The proposed methods are successfully applied for the simultaneous estimation of the residue of both pesticides in cucumber and cabbage samples.

scholarly journals UV Spectrophotometric Simultaneous Determination of Paracetamol and Ibuprofen in Combined Tablets by Derivative and Wavelet Transforms

2014 ◽  
Vol 2014 ◽  
pp. 1-13 ◽  
Author(s):  
Vu Dang Hoang ◽  
Dong Thi Ha Ly ◽  
Nguyen Huu Tho ◽  
Hue Minh Thi Nguyen
Keyword(s):  
Simultaneous Determination ◽  
Wavelet Transforms ◽  
Hybrid Approach ◽  
Uv Spectra ◽  
Order Derivative ◽  
First Order ◽  
Spectrophotometric Methods ◽  
Combined Use ◽  
Linear Concentration

The application of first-order derivative and wavelet transforms to UV spectra and ratio spectra was proposed for the simultaneous determination of ibuprofen and paracetamol in their combined tablets. A new hybrid approach on the combined use of first-order derivative and wavelet transforms to spectra was also discussed. In this application, DWT (sym6 and haar), CWT (mexh), and FWT were optimized to give the highest spectral recoveries. Calibration graphs in the linear concentration ranges of ibuprofen (12–32 mg/L) and paracetamol (20–40 mg/L) were obtained by measuring the amplitudes of the transformed signals. Our proposed spectrophotometric methods were statistically compared to HPLC in terms of precision and accuracy.

scholarly journals Application of Derivative Spectrophotometry for Simultaneous Determination of Quinapril and Hydrochlorothiazide in the Combination Tablets

2004 ◽  
Vol 87 (4) ◽  
pp. 847-851 ◽  
Author(s):  
Dorota Kowalczuk ◽  
Hanna Hopkała
Keyword(s):  
Standard Deviation ◽  
Simultaneous Determination ◽  
Relative Standard Deviation ◽  
Spectrophotometric Method ◽  
Derivative Spectrophotometry ◽  
Order Derivative ◽  
Zero Crossing ◽  
Relative Standard ◽  
Separation Of Components

Abstract A new second-order-derivative spectrophotometric method using zero-crossing technique measures quinapril (QUI) and hydrochlorothiazide (HYD) in 2-component mixtures. The procedure does not require prior separation of components from the sample. QUI was determined at a wavelength of 211.6 nm (zero-crossing wavelength point of HYD). Similarly, HYD was measured at 270.8 nm (zero-crossing wavelength point of QUI). Calibration graphs were constructed over the concentration range of 4.0 to 24.0 μ/mL for QUI and 2.5 to 15.0 μg/mL for HYD. Detection and quantitation limits were 0.85 and 2.5 μg/mL for QUI and 0.12 and 0.4 μg/mL for HYD, respectively. The accuracy (recovery 100.5–102%), precision (relative standard deviation less than 3.5% for QUI and 1.5% for HYD), selectivity, and sensitivity of the elaborated methods were satisfactory. The proposed method was applied successfully for the determination of both drugs in QUI-HYD tablets.

scholarly journals Simultaneous Determination of N-Butylscopolamine and Oxazepam in Pharmaceutical Formulations by First-Order Digital Derivative Spectrophotometry

2005 ◽  
Vol 88 (4) ◽  
pp. 1173-1178 ◽  
Author(s):  
Maria Inés Toral ◽  
Marcelo A Muñoz ◽  
Sandra L Orellana
Keyword(s):  
Standard Deviation ◽  
Simultaneous Determination ◽  
Relative Standard Deviation ◽  
Pharmaceutical Formulations ◽  
Derivative Spectrophotometry ◽  
Simple Method ◽  
Zero Crossing ◽  
First Order ◽  
Relative Standard

Abstract A simple method has been developed for the simultaneous determination of N-butylscopolamine bromide and oxazepam in pharmaceutical formulations using first-order digital derivative spectrophotometry. Acetonitrile was selected as the solvent in which both compounds showed well-defined bands. Both analytes showed good stability in this solvent when solutions of the analytes were exposed to light and temperatures between 20° and 80°C. The simultaneous determination of both drugs was performed by the zero-crossing method at 226.0 and 257.0 nm for N-butylscopolamine and oxazepam, respectively. The linear range of determination was found to be 2.5 × 10−7 to 8.0 × 10−5 mol/L for N-butylscopolamine and 7.1 × 10−8 to 8.0 × 10−5 mol/L for oxazepam. A very good level of repeatability (relative standard deviation) of 0.2% was observed for N-butylscopolamine and oxazepam. The ingredients commonly found in pharmaceutical formulations do not interfere. The proposed method was applied to the determination of these drugs in pharmaceutical formulations (capsules).

scholarly journals Simultaneous determination of amoxicillin and potassium clavulanate antibiotics in pharmacueutical sample using derivative spectrophotometric method

2011 ◽  
Vol 5 (3) ◽  
pp. 49-60
Author(s):  
Sarah S. Abdulameer ◽  
Khaleda H. Al-Saidi
Keyword(s):  
Simultaneous Determination ◽  
Second Order ◽  
Order Derivative ◽  
Zero Crossing ◽  
Accuracy And Precision ◽  
Amoxicillin Trihydrate ◽  
Potassium Clavulanate ◽  
2D And 3D ◽  
Very High

Derivatives spectrophotometric techniques were developed for the determination of Amoxicillin Trihydrate (Amox) with Potassium Clavulanate (PC) antibiotic binary mixtures. The simultaneous determination of these compounds was accomplished by derivative (1D, 2D and 3D) spectrophotometric technique and applying zero-crossing technique used for determination of (Amox) and (PC) in tablets. The second order derivative absorption spectra at valley λ=299 nm were used for (Amox) and also the second order derivative spectra at peak λ=239.5 nm were used for (PC). No interferences were found between both determined constituents and those of matrix. A good accuracy and precision of simultaneous determination of (Amox) and (PC) were confirmed by statistical analysis. The recovery of individual constituents under established conditions is very high and ranges for synthetic standards mixture and tablets from 100.11, 99.33 and 96.98, 96.84 respectively. Linearity is maintained within a wide concentration range from 2.0 to 90.0 μg.mL-1 and from 10.0 to 90.0 μg.mL-1 for (Amox) and (PC) and linearity percentage 99.98 and 99.99 respectively. The detection limit is 0.211 μg.mL-1 for (Amox) and 0.259 μg.mL-1 for (PC). The corresponding quantitation limits are 0.704 μg.mL-1 (Amox) and 0.864 μg.mL-1 for (PC).

scholarly journals UV-Spectrophotometric Estimation of Olopatadine hydrochloride in Bulk and Pharmaceutical Dosage Form by Zero, First and Second Order Derivative Methods

2019 ◽  
Vol 9 (4-s) ◽  
pp. 519-524
Author(s):  
Smita. S. Aher ◽  
Jayshree S. Gawali ◽  
Ravindranath B. Saudagar
Keyword(s):  
Dosage Form ◽  
Low Cost ◽  
Second Order ◽  
Order Derivative ◽  
Pharmaceutical Dosage Form ◽  
Pharmaceutical Dosage Forms ◽  
Spectrophotometric Methods ◽  
Relative Standard ◽  
Derivative Method ◽  
Olopatadine Hydrochloride

Simple and accurate UV spectrophotometric methods by Zero, First and Second order derivative method  have been developed and validated for the estimation of Olopatadine hydrochloride in bulk and its pharmaceutical dosage form. The standard and sample solutions of Olopatadine hydrochloride were prepared in methanol and water. Olopatadine hydrochloride was estimated at 299, 289 and 267 nm for the  derivative UV-spectrophotometric method. Beer’s law was obeyed in the concentration range of 20 to 120 μg / mL with coefficient of correlation value 0.9996, 0.999 and 0.999 for Zero, First and Second order derivative method. These methods were tested and validated for various parameters according to ICH and USP guidelines. The precision expressed as relative standard deviation were of less than 2 for the above three methods respectively. The proposed methods were successfully applied for the determination of Olopatadine hydrochloride in pharmaceutical dosage form. Results of the analysis were validated statistically and were found to be satisfactory. The proposed methods are simple, easy to apply, low-cost and require relatively inexpensive instruments. Keywords: Olopatadine HCl, UV-Visible spectrophotometry, Pharmaceutical Dosage forms, Derivative Spectroscopy, Method validation.

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