scholarly journals The Inhibitory Effect of 3β-Hydroxy-12-oleanen-27-oic Acid on Growth and Motility of Human Hepatoma HepG2 Cells through JNK and Akt Signaling Pathway

2013 ◽  
Vol 2013 ◽  
pp. 1-17 ◽  
Author(s):  
Juanjuan Wang ◽  
Xiangfeng Chen ◽  
Zhihua Zhou ◽  
Jinhui Li ◽  
Hongxiang Sun
Keyword(s):  
Hepg2 Cells ◽  
Molecular Mechanisms ◽  
Inhibitory Effect ◽  
Pcr Array ◽  
Human Hepatoma ◽  
Antitumor Effects ◽  
Human Hepatoma Hepg2 Cells ◽  
Hepatoma Hepg2

3β-Hydroxy-12-oleanen-27-oic acid (ATA) was a main antitumor active triterpene from the rhizomes ofAstilbe chinensis. In this study, we investigated its effects on growth, apoptosis, cell cycle, motility/invasion, and metatasis in human hepatoma HepG2 cellsin vitroand antimetastasis of B16-F10 melanoma in micein vivo, as well as its molecular mechanisms of action using a high-throughput Cancer Pathway Finder PCR Array. ATA could not only induce tumor cells into apoptosis through the activation of both extrinsic and intrinsic pathways, arrest HepG2 cells in G2/M phase, but also suppress the invasion and metastasis abilities of HepG2 cells and the lung metastasis of B16-F10 melanoma in mice. PCR array assay revealed that ATA upregulated 9 genes including CDKN1A, MDM2, CFLAR (CASPER), TNFRSF10B (DR5), c-Jun, IL-8, THBS1, SERPINB5 (maspin), and TNF and downregulated 8 genes such as CCNE1, AKT, ANGPT1, TEK, TGFBR1, MMP9, U-PA, and S100A4. These results indicate that ATA could exert antitumor effects through activating JNK/MAPK and suppressing AKT signal transduction pathways and that ATA might be a potent anticancer agent.

In vitro study of ultrasound on multidrug resistance in MDR human hepatoma HepG2 cells

2008 ◽  
Vol 5 (3) ◽  
pp. 165-171
Author(s):  
Qiujun Qi ◽  
Baojin Zhai ◽  
Yumian Guo ◽  
Zhihong Wang ◽  
Feng Wu
Keyword(s):  
Multidrug Resistance ◽  
Hepg2 Cells ◽  
In Vitro Study ◽  
Vitro Study ◽  
Human Hepatoma ◽  
Human Hepatoma Hepg2 Cells ◽  
Hepatoma Hepg2

scholarly journals In vitro infection of human hepatoma (HepG2) cells with hepatitis B virus.

1990 ◽  
Vol 64 (6) ◽  
pp. 3025-3032 ◽  
Author(s):  
R Bchini ◽  
F Capel ◽  
C Dauguet ◽  
S Dubanchet ◽  
M A Petit
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Hepg2 Cells ◽  
Human Hepatoma ◽  
B Virus ◽  
Human Hepatoma Hepg2 Cells ◽  
Hepatoma Hepg2

scholarly journals Xanthomicrol Exerts Antiangiogenic and Antitumor Effects in a Mouse Melanoma (B16F10) Allograft Model

2020 ◽  
Vol 2020 ◽  
pp. 1-11
Author(s):  
Foad Ghazizadeh ◽  
Massoumeh Shafiei ◽  
Reza Falak ◽  
Mahshid Panahi ◽  
Naser Rakhshani ◽  
...  
Keyword(s):  
Molecular Mechanisms ◽  
Inhibitory Effect ◽  
Vehicle Group ◽  
B16f10 Melanoma ◽  
Antitumor Effects ◽  
Mouse Melanoma ◽  
Phosphorylated Akt ◽  
Melanoma B16f10

Xanthomicrol, a trimethoxylated hydroxyflavone, is the main active component of Dracocephalum kotschyi Boiss leaf extract. Preliminary in vitro studies identified this compound as a potential antiangiogenic and anticancer agent. This study aimed to evaluate in vivo anticancer effect of xanthomicrol and investigate its molecular mechanism of action in a mouse melanoma (B16F10) model. Effect of xanthomicrol on B16F10 melanoma cell viability was determined using the MTT assay. For in vivo experiments, C57BL/6 mice were inoculated subcutaneously with B16F10 cells. After five days, once daily administration of xanthomicrol, thalidomide, or vehicle was commenced and continued for 21 consecutive days. On the 26th day, blood samples and tumor biopsies were taken for subsequent molecular analysis. Xanthomicrol showed inhibitory effect on viability of B16F10 melanoma cells (IC50 value: 3.433 μg/ml). Initial tumor growth, tumor volume and weight, and angiogenesis were significantly decreased in xanthomicrol-treated animals compared with those in vehicle group. Protein expression of phosphorylated Akt, mRNA expressions of HIF-1α and VEGF in tumor tissues, and serum VEGF were significantly decreased in xanthomicrol-treated animals compared with vehicle-treated animals. Thus, xanthomicrol inhibited cancer cell growth both in vitro and in vivo. This effect, at least in part, was exerted by interfering with PI3K/Akt signaling pathway and inhibiting VEGF secretion by tumor cells. Further studies are required to elucidate the exact molecular mechanisms of antitumor activity of xanthomicrol.

scholarly journals Hepatoprotection and Phytochemistry of the Vietnamese Herbs Cleome chelidonii and Cleome viscosa Stems

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Nhat Minh Phan ◽  
Thi Hong Tuoi Do ◽  
Le Thanh Tuyen Nguyen ◽  
Trong Tuan Nguyen ◽  
Quoc Luan Ngo ◽  
...  
Keyword(s):  
Body Weight ◽  
Carbon Tetrachloride ◽  
Alanine Aminotransferase ◽  
Liver Toxicity ◽  
Human Hepatoma ◽  
Human Hepatoma Hepg2 Cells ◽  
Phytochemical Studies ◽  
Hepatoma Hepg2

The study aims to determine the hepatoprotective effect of n-hexane, ethyl acetate, and methanol extracts of the leaves and stems of two Cleome species against carbon tetrachloride- (CCl4-) induced liver toxicity both in vitro using human hepatoma (HepG2) cells and in vivo in rats as well as the hepatoprotective property of all isolated compounds on HepG2. After 72 h of treatment, at the concentrations of 25, 50, and 100 μg/mL, the methanol of C. chelidonii stems (CCSM) ranged from 18.6% to 20.8%, whereas the methanol of C. chelidonii stems (CVSM) increased from 12.3% to 17.2% cell viability. The results show that CCSM and CVSM significantly expressed in vitro hepaprotective activity on HepG2. Therefore, the animals were daily treated with these extracts at the doses of 15, 30, and 45 mg/kg body weight for 5 days, and CCl4 was injected (2 ml/kg body weight, i.p.) on the 2nd and 3rd days. Levels of aspartate aminotransferase (ALT) and alanine aminotransferase (AST) in the blood were measured and compared to the silymarin control. The treatments with CCSM and CVSM (30, and 45 mg/kg) possessed significant hepatoprotection and were comparable with the activity of silymarin. Further, phytochemical studies of these ones were conducted and led to the identification of eight flavonoids: visconoside A (1), visconoside B (2), quercetin 3-O-β-D-glucopyranoside 7-O-α-L-rhamnopyranoside (3), kaempferol 3-O-β-D-glucopyranoside 7-O-α-L-rhamnopyranoside (4), cleomeside A (5), cleomeside B (6), cleomeside C (7), and quercetin-3-O-[β-D-glucopyranosyl-(1⟶2)]-α-L-rhamnopyranoside 7-O-α-L-rhamnopyranoside (8). Two major flavonoids (1 and 4) displayed significant hepatoprotective property (at the concentration of 100 μM, the prevention percentage values were 66.5% and 74.2%, respectively, compared to the quercetin control, with value of 80.3%).

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